{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:38:13Z","timestamp":1789951093504,"version":"4.0.1"},"reference-count":46,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Structural Biology"],"published-print":{"date-parts":[[2011,12]]},"DOI":"10.1016\/j.jsb.2011.09.013","type":"journal-article","created":{"date-parts":[[2011,10,15]],"date-time":"2011-10-15T14:08:34Z","timestamp":1318687714000},"page":"350-359","source":"Crossref","is-referenced-by-count":20,"title":["Cobblestone HUVECs: A human model system for studying primary ciliogenesis"],"prefix":"10.1016","volume":"176","author":[{"given":"Willie J.C.","family":"Geerts","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Vocking","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natasja","family":"Schoonen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsay","family":"Haarbosch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elly G.","family":"van Donselaar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsa","family":"Regan-Klapisz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan Andries","family":"Post","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jsb.2011.09.013_b0005","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.cellbi.2003.11.013","article-title":"Vertebrate primary cilia: a sensory part of centrosomal complex in tissue cells, but a \u201csleeping beauty\u201d in cultured cells?","volume":"28","author":"Alieva","year":"2004","journal-title":"Cell Biol. Int."},{"key":"10.1016\/j.jsb.2011.09.013_b0010","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1076\/ejom.39.5.277.7380","article-title":"The ultrastructure of primary cilia in the endocrine and excretory duct cells of the pancreas of mice and rats","volume":"39","author":"Aughsteen","year":"2001","journal-title":"Eur. J. Morphol."},{"key":"10.1016\/j.jsb.2011.09.013_b0015","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1146\/annurev.genom.7.080505.115610","article-title":"The ciliopathies: an emerging class of human genetic disorders","volume":"7","author":"Badano","year":"2006","journal-title":"Annu. Rev. Genomics Hum. Genet"},{"key":"10.1016\/j.jsb.2011.09.013_b0020","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.cellbi.2008.03.001","article-title":"The pipe and the pinwheel: is pressure an effective stimulus for the 9+0 primary cilium?","volume":"32","author":"Bell","year":"2008","journal-title":"Cell Biol. Int."},{"key":"10.1016\/j.jsb.2011.09.013_b0025","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/j.cub.2009.05.025","article-title":"The primary cilium as a complex signaling center","volume":"19","author":"Berbari","year":"2009","journal-title":"Curr. Biol."},{"key":"10.1016\/j.jsb.2011.09.013_b0035","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1111\/j.1600-0854.2006.00516.x","article-title":"Sensory cilia and integration of signal transduction in human health and disease","volume":"8","author":"Christensen","year":"2007","journal-title":"Traffic"},{"key":"10.1016\/j.jsb.2011.09.013_b0040","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/S0070-2153(08)00810-7","article-title":"The primary cilium coordinates signaling pathways in cell cycle control and migration during development and tissue repair","volume":"85","author":"Christensen","year":"2008","journal-title":"Curr. Top. Dev. Biol."},{"key":"10.1016\/j.jsb.2011.09.013_b0045","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1242\/jcs.03305","article-title":"Centriole\/basal body morphogenesis and migration during ciliogenesis in animal cells","volume":"120","author":"Dawe","year":"2007","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.jsb.2011.09.013_b0050","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1002\/elps.200500746","article-title":"Protein expression dynamics during replicative senescence of endothelial cells studied by 2-D difference in gel electrophoresis","volume":"27","author":"Eman","year":"2006","journal-title":"Electrophoresis"},{"key":"10.1016\/j.jsb.2011.09.013_b0055","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1038\/nrm2278","article-title":"When cilia go bad: cilia defects and ciliopathies","volume":"8","author":"Fliegauf","year":"2007","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.jsb.2011.09.013_b0060","doi-asserted-by":"crossref","first-page":"3781","DOI":"10.1091\/mbc.E06-02-0133","article-title":"The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly","volume":"17","author":"Follit","year":"2006","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.jsb.2011.09.013_b0065","series-title":"Handbook of Cryo-Preparation Methods for Electron Microscopy","first-page":"617","article-title":"3D electron tomography of cells and organelles","author":"Geerts","year":"2009"},{"key":"10.1016\/j.jsb.2011.09.013_b0070","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.cell.2009.03.023","article-title":"The vertebrate primary cilium in development, homeostasis, and disease","volume":"137","author":"Gerdes","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.jsb.2011.09.013_b0080","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s007950000009","article-title":"Ciliogenesis and ciliary abnormalities Med","volume":"33","author":"Hagiwara","year":"2000","journal-title":"Electron Micros."},{"key":"10.1016\/j.jsb.2011.09.013_b0075","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/s00795-008-0421-z","article-title":"The primary cilia of secretory cells in the human oviduct mucosa","volume":"41","author":"Hagiwara","year":"2008","journal-title":"Med. Mol. Morphol."},{"key":"10.1016\/j.jsb.2011.09.013_b0085","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1038\/nrg1727","article-title":"Cilia and centrosomes: a unifying pathogenic concept for cystic kidney disease?","volume":"6","author":"Hildebrandt","year":"2005","journal-title":"Nature Rev."},{"issue":"6","key":"10.1016\/j.jsb.2011.09.013_b0090","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1083\/jcb.200312133","article-title":"Primary cilia of human endothelial cells disassemble under laminar shear stress","volume":"164","author":"Iomini","year":"2004","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.jsb.2011.09.013_b0095","doi-asserted-by":"crossref","first-page":"2745","DOI":"10.1172\/JCI107470","article-title":"Culture of human endothelial cells derived from umbilical veins. Identification by morphological and immunologic criteria","volume":"52","author":"Jaffe","year":"1973","journal-title":"The J. Clin. Investig."},{"key":"10.1016\/j.jsb.2011.09.013_b0100","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1111\/j.1365-2818.2006.01558.x","article-title":"Aclar discs: a versatile substrate for routine high-pressure freezing of mammalian cell monolayers","volume":"221","author":"Jim\u00e9nez","year":"2006","journal-title":"J. Micros."},{"key":"10.1016\/j.jsb.2011.09.013_b0105","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.jsb.2008.12.009","article-title":"Tannic acid-mediated osmium impregnation after freeze-substitution: a strategy to enhance membrane contrast for electron tomography","volume":"166","author":"Jim\u00e9nez","year":"2009","journal-title":"J. Struct. Biol."},{"issue":"3","key":"10.1016\/j.jsb.2011.09.013_b0110","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1083\/jcb.201101005","article-title":"Regulating the transition from centriole to basal body","volume":"193","author":"Kobayashi","year":"2011","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.jsb.2011.09.013_b0115","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1006\/jsbi.1996.0013","article-title":"Computer visualization of three-dimensional image data using IMOD","volume":"116","author":"Kremer","year":"1996","journal-title":"J. Struct. Biol."},{"key":"10.1016\/j.jsb.2011.09.013_b0125","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1083\/jcb.200106141","article-title":"Intraflagellar transport balances continuous turnover of outer doublet microtubules: implications for flagellar length control","volume":"155","author":"Marshall","year":"2001","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.jsb.2011.09.013_b0130","doi-asserted-by":"crossref","first-page":"6463","DOI":"10.1158\/0008-5472.CAN-06-0462","article-title":"The primary cilium in cell signaling and cancer","volume":"66","author":"Michaud","year":"2006","journal-title":"Cancer Res."},{"key":"10.1016\/j.jsb.2011.09.013_b0135","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1242\/jcs.059519","article-title":"The ciliary pocket: an endocytic membrane domain at the base of primary and motile cilia","volume":"123","author":"Molla-Herman","year":"2010","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.jsb.2011.09.013_b0140","doi-asserted-by":"crossref","first-page":"2802","DOI":"10.1016\/j.yexcr.2009.06.028","article-title":"Adenylate cyclase regulates elongation of mammalian primary cilia","volume":"315","author":"Ou","year":"2009","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.jsb.2011.09.013_b0145","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0070-2153(08)00805-3","article-title":"Targeting proteins to the ciliary membrane","volume":"85","author":"Pazour","year":"2008","journal-title":"Curr. Top. Dev. Biol."},{"key":"10.1016\/j.jsb.2011.09.013_b0150","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1083\/jcb.151.3.709","article-title":"Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene Tg737, are required for assembly of cilia and flagella","volume":"151","author":"Pazour","year":"2000","journal-title":"The J. Cell Biol."},{"key":"10.1016\/j.jsb.2011.09.013_b0155","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0070-2153(08)00802-8","article-title":"Intraflagellar transport (IFT) role in ciliary assembly, resorption and signaling","volume":"85","author":"Pedersen","year":"2008","journal-title":"Curr. Top. Dev. Biol."},{"key":"10.1016\/j.jsb.2011.09.013_b0160","doi-asserted-by":"crossref","first-page":"4225","DOI":"10.1091\/mbc.E08-02-0210","article-title":"Golgi-associated cPLA2\u03b1 regulates endothelial cell-cell junction integrity by controlling the trafficking of transmembrane junction proteins","volume":"20","author":"Regan-Klapisz","year":"2009","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.jsb.2011.09.013_b0165","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1038\/nrm952","article-title":"Intraflagellar transport","volume":"3","author":"Rosenbaum","year":"2002","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.jsb.2011.09.013_b0170","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1146\/annurev.physiol.69.040705.141236","article-title":"Overview of structure and function of mammalian cilia","volume":"69","author":"Satir","year":"2007","journal-title":"Annu. Rev. Physiol."},{"key":"10.1016\/j.jsb.2011.09.013_b0175","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1083\/jcb.200709133","article-title":"Intraflagellar transport motors in cilia: moving along the cell\u2019s antenna","volume":"180","author":"Scholey","year":"2008","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.jsb.2011.09.013_b0180","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.cell.2006.04.013","article-title":"Intraflagellar transport and cilium-based signaling","volume":"125","author":"Scholey","year":"2006","journal-title":"Cell"},{"key":"10.1016\/j.jsb.2011.09.013_b0185","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/S0070-2153(08)00813-2","article-title":"Ciliary dysfunction in developmental abnormalities and diseases","volume":"85","author":"Sharma","year":"2008","journal-title":"Curr. Top. Dev. Biol."},{"key":"10.1016\/j.jsb.2011.09.013_b0190","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1098\/rspb.1976.0031","article-title":"Gametogenesis and fertilization in Plasmodium yoellii nigeriensis: a transmission electron microscope study","volume":"193","author":"Sinden","year":"1976","journal-title":"Proc. R. Soc Lond. B"},{"key":"10.1016\/j.jsb.2011.09.013_b0195","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1126\/science.1124534","article-title":"The primary cilium as the cell\u2019s antenna: signaling at a sensory organelle","volume":"313","author":"Singla","year":"2006","journal-title":"Science"},{"key":"10.1016\/j.jsb.2011.09.013_b0200","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/S0046-8177(86)80206-4","article-title":"Ultrastructural abnormalities of cilia in the human respiratory tract","volume":"17","author":"Smallman","year":"1986","journal-title":"Hum. Pathol."},{"key":"10.1016\/j.jsb.2011.09.013_b0205","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1083\/jcb.15.2.363","article-title":"Centrioles and the formation of rudimentary cilia by fibroblasts and smooth muscle cells","volume":"15","author":"Sorokin","year":"1962","journal-title":"The J. Cell Biol."},{"key":"10.1016\/j.jsb.2011.09.013_b0210","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1242\/jcs.3.2.207","article-title":"Reconstructions of centriole formation and ciliogenesis in mammalian lungs","volume":"3","author":"Sorokin","year":"1968","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.jsb.2011.09.013_b0215","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1002\/dvdy.20557","article-title":"Monocilia on chicken embryonic endocardium in low shear stress areas","volume":"235","author":"Van der Heiden","year":"2006","journal-title":"Dev. Dyn."},{"key":"10.1016\/j.jsb.2011.09.013_b0220","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/j.atherosclerosis.2007.05.030","article-title":"Endothelial primary ciia in areas of disturbed flow are at the base of atherosclerosis","volume":"196","author":"Van der Heiden","year":"2008","journal-title":"Atherosclerosis"},{"key":"10.1016\/j.jsb.2011.09.013_b0225","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/BF00215830","article-title":"Single modified cilia displayed by cells of human internal stratified epithelia (oral cavity, vagina)","volume":"251","author":"Warfvinge","year":"1988","journal-title":"Cell Tissue Res."},{"key":"10.1016\/j.jsb.2011.09.013_b0230","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1159\/000163955","article-title":"Primary cilia in normal and pathological tissues","volume":"63","author":"Wheatley","year":"1995","journal-title":"Pathobiology"},{"key":"10.1016\/j.jsb.2011.09.013_b0235","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/S0248-4900(00)01108-4","article-title":"Length control of primary cilia: analysis of monociliate and multiciliate PtK1 Cells","volume":"92","author":"Wheatley","year":"2000","journal-title":"Biol. Cell"},{"key":"10.1016\/j.jsb.2011.09.013_b0240","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1006\/cbir.1996.0011","article-title":"Expression of primary cilia in mammalian cells","volume":"20","author":"Wheatley","year":"1996","journal-title":"Cell Biol. Int."}],"container-title":["Journal of Structural Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S104784771100284X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S104784771100284X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,12,11]],"date-time":"2021-12-11T03:51:36Z","timestamp":1639194696000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S104784771100284X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["S104784771100284X"],"URL":"https:\/\/doi.org\/10.1016\/j.jsb.2011.09.013","relation":{},"ISSN":["1047-8477"],"issn-type":[{"value":"1047-8477","type":"print"}],"subject":[],"published":{"date-parts":[[2011,12]]}}}