{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:45:53Z","timestamp":1791431153293,"version":"4.3.3"},"reference-count":49,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"FEDER","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Structural Biology"],"published-print":{"date-parts":[[2015,12]]},"DOI":"10.1016\/j.jsb.2015.10.012","type":"journal-article","created":{"date-parts":[[2015,10,23]],"date-time":"2015-10-23T19:44:34Z","timestamp":1445629474000},"page":"500-509","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":40,"title":["Nacre extract restores the mineralization capacity of subchondral osteoarthritis osteoblasts"],"prefix":"10.1016","volume":"192","author":[{"given":"A.","family":"Brion","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Dossot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Moby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Dumas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Hupont","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.H.","family":"Piet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Bianchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Mainard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Galois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Gillet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Rousseau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jsb.2015.10.012_b0005","doi-asserted-by":"crossref","DOI":"10.1007\/BF02065189","article-title":"Recalcification of decalcified bone collagen in vitro as a model for biologic calcification","author":"Bachra","year":"1968","journal-title":"Calcif. Tissue Res."},{"issue":"4","key":"10.1016\/j.jsb.2015.10.012_b0010","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1007\/s10126-007-7120-y","article-title":"Heterogeneity of proteinase inhibitors in the water-soluble organic matrix from the oyster nacre","volume":"9","author":"B\u00e9douet","year":"2007","journal-title":"Mar. Biotechnol. (NY)"},{"issue":"1","key":"10.1016\/j.jsb.2015.10.012_b0015","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1007\/BF02058648","article-title":"An electron microscope study of the formation of the nacreous layer in the shell of certain bivalve molluscs","volume":"3","author":"Bevelander","year":"1969","journal-title":"Calcif. Tissue Res."},{"issue":"3","key":"10.1016\/j.jsb.2015.10.012_b0020","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S8756-3282(98)00092-1","article-title":"Fourier transform infrared microspectroscopic analysis of bones of osteocalcin-deficient mice provides insight into the function of osteocalcin","volume":"23","author":"Boskey","year":"1998","journal-title":"Bone"},{"issue":"12","key":"10.1016\/j.jsb.2015.10.012_b0025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0084584","article-title":"Water soluble bioactives of nacre mediate antioxidant activity and osteoblast differentiation","volume":"8","author":"Chaturvedi","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jsb.2015.10.012_b0030","article-title":"Mineralisation and osteoblast cells response of nanograde pearl powders","author":"Chen","year":"2013","journal-title":"J. Nanomater."},{"issue":"3","key":"10.1016\/j.jsb.2015.10.012_b0035","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.bcp.2011.11.024","article-title":"Heme oxygenase-1 mediates protective effects on inflammatory, catabolic and senescence responses induced by interleukin-1\u00e2 in osteoarthritic osteoblasts","volume":"83","author":"Cl\u00e9rigues","year":"2012","journal-title":"Biochem. Pharmacol."},{"issue":"5","key":"10.1016\/j.jsb.2015.10.012_b0040","doi-asserted-by":"crossref","first-page":"684","DOI":"10.3899\/jrheum.120845","article-title":"RANKL\/OPG ratio and DKK-1 expression in primary osteoblastic cultures from osteoarthritic and osteoporotic subjects","volume":"40","author":"Corrado","year":"2013","journal-title":"J. Rheumatol."},{"issue":"5","key":"10.1016\/j.jsb.2015.10.012_b0045","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.1002\/art.24489","article-title":"Altered mineralization of human osteoarthritic osteoblasts is attributable to abnormal type I collagen production","volume":"60","author":"Couchourel","year":"2009","journal-title":"Arthritis Rheum."},{"issue":"5","key":"10.1016\/j.jsb.2015.10.012_b0050","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1016\/j.bone.2012.01.024","article-title":"Decreased bone tissue mineralisation can partly explain subchondral sclerosis observed in osteoarthritis","volume":"50","author":"Cox","year":"2012","journal-title":"Bone"},{"key":"10.1016\/j.jsb.2015.10.012_b0055","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.bone.2006.02.059","article-title":"Raman spectroscopy evidence for octacalcium phosphate and other transient mineral species deposited during intramembranous mineralization","volume":"39","author":"Crane","year":"2006","journal-title":"Bone"},{"key":"10.1016\/j.jsb.2015.10.012_b0060","unstructured":"Dumas, D., Hupont, S., Improved biomedical imaging with multiphoton excitation. WO 2012160312 A3, Patent published in 2013."},{"key":"10.1016\/j.jsb.2015.10.012_b0065","doi-asserted-by":"crossref","first-page":"485","DOI":"10.4236\/eng.2014.68050","article-title":"Non-invasive Second Harmonic Generation (SHG) in Macroscopy (MacroSHG) as bio-diagnosis to image collagen network organization in extracellular matrix","volume":"6","author":"Dumas","year":"2014","journal-title":"Engineering"},{"key":"10.1016\/j.jsb.2015.10.012_b0070","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.exger.2014.03.010","article-title":"Influence of age on osteoarthritis progression after anterior cruciate ligament transaction in rats","volume":"55","author":"Ferr\u00e1ndiz","year":"2014","journal-title":"Exp. Gerontol."},{"key":"10.1016\/j.jsb.2015.10.012_b0075","article-title":"Chondroprotection and the prevention of osteoarthritis progression of the knee: a systematic review of treatment agents","author":"Gallagher","year":"2014","journal-title":"Am. J. Sports Med."},{"issue":"9","key":"10.1016\/j.jsb.2015.10.012_b0080","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1002\/jrs.1734","article-title":"Reference database of Raman spectra of biological molecules","volume":"38","author":"Gelder","year":"2007","journal-title":"J. Raman Spectrosc."},{"issue":"1","key":"10.1016\/j.jsb.2015.10.012_b0085","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1039\/C3AN01716H","article-title":"Monitoring the mineralisation of bone nodules in vitro by space- and time-resolved Raman micro-spectroscopy","volume":"139","author":"Ghita","year":"2014","journal-title":"Analyst"},{"key":"10.1016\/j.jsb.2015.10.012_b0090","series-title":"Metabolic Bone Disease and Clinically Related Disorders","first-page":"23","article-title":"The nature of mineral phase in bone, biological and clinical implications","author":"Glimcher","year":"1998"},{"key":"10.1016\/j.jsb.2015.10.012_b0095","doi-asserted-by":"crossref","DOI":"10.14348\/molcells.2015.2315","article-title":"Osteogenic potency of nacre on human mesenchymal stem cells","author":"Green","year":"2015","journal-title":"Mol. Cells"},{"issue":"1","key":"10.1016\/j.jsb.2015.10.012_b0100","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.ab.2004.02.002","article-title":"An Alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction","volume":"329","author":"Gregory","year":"2004","journal-title":"Anal. Biochem."},{"issue":"4","key":"10.1016\/j.jsb.2015.10.012_b0105","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1016\/S0002-9440(10)63208-7","article-title":"Concerted regulation ofinorganicpyrophosphateandosteopontinbyakp2, enpp1, and ank: an integrated model of the pathogenesis of mineralization disorders","volume":"164","author":"Harmey","year":"2004","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.jsb.2015.10.012_b0110","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1002\/1529-0131(199805)41:5<891::AID-ART17>3.0.CO;2-X","article-title":"Osteoblast-like cells from human subchondral osteoarthritic bone demonstrate an altered phenotype in vitro: possible role in subchondral bone sclerosis","volume":"41","author":"Hilal","year":"1998","journal-title":"Arthritis Rheum."},{"key":"10.1016\/j.jsb.2015.10.012_b0115","first-page":"125","article-title":"Osteopontin and Osteocalcin in Maxilla Tissue of Skeletal Class III Patients","volume":"11","author":"Jankovska","year":"2009","journal-title":"Stomatologija, Baltic Dental and Maxillofacial J."},{"issue":"30","key":"10.1016\/j.jsb.2015.10.012_b0120","doi-asserted-by":"crossref","first-page":"7489","DOI":"10.1016\/j.biomaterials.2012.06.098","article-title":"The role of nacreous factors in preventing osteoporotic bone loss through both osteoblast activation and osteoclast inactivation","volume":"33","author":"Kim","year":"2012","journal-title":"Biomaterials"},{"key":"10.1016\/j.jsb.2015.10.012_b0125","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s00441-009-0832-8","article-title":"Regulation of bone development and extracellular matrix protein genes by RUNX2","volume":"339","author":"Komori","year":"2010","journal-title":"Cell Tissue Res."},{"key":"10.1016\/j.jsb.2015.10.012_b0130","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1172\/JCI118984","article-title":"Demonstration of an osteoblast defect in two cases of human malignant osteopetrosis: correction of the phenotype after bone marrow transplant","volume":"98","author":"Lajeunesse","year":"1996","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.jsb.2015.10.012_b0135","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/0169-6009(91)90025-U","article-title":"Regulation of osteocalcin secretion by human primary bone cells and by the human osteosarcoma cell line MG-63","volume":"14","author":"Lajeunesse","year":"1991","journal-title":"Bone Miner."},{"key":"10.1016\/j.jsb.2015.10.012_b0140","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/S0142-9612(00)00213-1","article-title":"Bone reactions to nacre injected percutaneously into the vertebrae of sheep","volume":"22","author":"Lamghari","year":"2001","journal-title":"Biomaterials"},{"key":"10.1016\/j.jsb.2015.10.012_b0145","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using realtime quantitative PCR and the 2(\u2212Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"issue":"1","key":"10.1016\/j.jsb.2015.10.012_b0150","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1054\/tice.1999.0091","article-title":"Stimulation of rat cutaneous fibroblasts and their synthetic activity by implants of powdered nacre (mother of pearl)","volume":"32","author":"Lopez","year":"2000","journal-title":"Tissue Cell"},{"issue":"5","key":"10.1016\/j.jsb.2015.10.012_b0155","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1016\/0040-8166(92)90037-8","article-title":"Demonstration of the capacity of nacre to induce bone formation by human osteoblasts maintained in vitro","volume":"24","author":"Lopez","year":"1992","journal-title":"Tissue Cell"},{"issue":"7","key":"10.1016\/j.jsb.2015.10.012_b0160","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1359\/jbmr.2003.18.7.1186","article-title":"Bone morphogenetic protein-2 restores mineralization in glucocorticoid-inhibited MC3T3-E1 osteoblast cultures","volume":"18","author":"Luppen","year":"2003","journal-title":"J. Bone Miner. Res."},{"issue":"1893","key":"10.1016\/j.jsb.2015.10.012_b0165","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1098\/rsta.2009.0007","article-title":"Biomimetic design of materials and biomaterials inspired by the structure of nacre","volume":"367","author":"Luz","year":"2009","journal-title":"Philos. Trans. A Math. Phys. Eng. Sci."},{"issue":"9","key":"10.1016\/j.jsb.2015.10.012_b0170","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1101\/gad.1276205","article-title":"Unique coexpression in osteoblasts of broadly expressed genes accounts for the spatial restriction of ECM mineralization to bone","volume":"19","author":"Murshed","year":"2005","journal-title":"Genes Dev."},{"issue":"1","key":"10.1016\/j.jsb.2015.10.012_b0175","doi-asserted-by":"crossref","first-page":"99","DOI":"10.20344\/amp.5477","article-title":"Osteoarthritis","volume":"28","author":"Pereira","year":"2015","journal-title":"Acta Med. Port."},{"issue":"2","key":"10.1016\/j.jsb.2015.10.012_b0180","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1002\/jbm.a.31553","article-title":"Low molecular weight molecules of oyster nacre induce mineralization of the MC3T3-E1 cells","volume":"85","author":"Rousseau","year":"2008","journal-title":"J. Biomed. Mater. Res. A"},{"key":"10.1016\/j.jsb.2015.10.012_b0185","first-page":"1","article-title":"The water soluble matrix fraction from the nacre of Pinctada maxima produces earlier mineralization of MC3T3-E1 mouse pre-osteoblasts","volume":"135","author":"Rousseau","year":"2003","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.jsb.2015.10.012_b0190","doi-asserted-by":"crossref","first-page":"1731","DOI":"10.1021\/cm960429i","article-title":"Does abalone nacre form by heteroepitaxial nucleation or by growth through mineral bridges?","volume":"9","author":"Sch\u00e4ffer","year":"1997","journal-title":"Chem. Mater."},{"issue":"16","key":"10.1016\/j.jsb.2015.10.012_b0195","doi-asserted-by":"crossref","first-page":"9420","DOI":"10.1074\/jbc.270.16.9420","article-title":"Rapidly forming apatitic mineral in an osteoblastic cell line (UMR 106-01 BSP)","volume":"270","author":"Stanford","year":"1995","journal-title":"J. Biol. Chem."},{"issue":"1","key":"10.1016\/j.jsb.2015.10.012_b0200","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.bone.2013.05.003","article-title":"Age-related bone deterioration is diminished by disrupted collagen sensing in integrin \u03b12\u03b21 deficient mice","volume":"56","author":"Stange","year":"2013","journal-title":"Bone"},{"key":"10.1016\/j.jsb.2015.10.012_b0205","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1042\/bj2660015","article-title":"Isolation and characterization of proteoglycans synthesized by mouse osteoblastic cells in culture during the mineralization process","volume":"266","author":"Takeuchi","year":"1990","journal-title":"Biochem. J."},{"issue":"6","key":"10.1016\/j.jsb.2015.10.012_b0215","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1359\/jbmr.2002.17.6.1118","article-title":"Mineralization of developing mouse calvaria as revealed by Raman microspectroscopy","volume":"17","author":"Tarnowski","year":"2002","journal-title":"J. Bone Miner. Res."},{"issue":"1","key":"10.1016\/j.jsb.2015.10.012_b0220","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.berh.2009.08.004","article-title":"Targeting subchondral bone for treating osteoarthritis: what is the evidence?","volume":"24","author":"Tat","year":"2010","journal-title":"Best Pract. Res. Clin. Rheumatol."},{"issue":"3","key":"10.1016\/j.jsb.2015.10.012_b0225","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.bone.2011.06.005","article-title":"Strontium ranelate inhibits key factors affecting bone remodeling in human osteoarthritic subchondral bone osteoblasts","volume":"49","author":"Tat","year":"2011","journal-title":"Bone"},{"key":"10.1016\/j.jsb.2015.10.012_b0230","unstructured":"Tsai, M.T., Lin, Y.S., Chen, W.C., Ho, C.H., Huang, H.L., Hsu, J.T., 2011. Runx2 and Osterix gene expression in human bone marrow stromal cells are mediated by far-infrared radiation. In: Proceedings of the World Congress on Engineering 2011 Vol III WCE July 6\u20138, 2011, London, U.K."},{"key":"10.1016\/j.jsb.2015.10.012_b0235","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.cbpb.2010.11.004","article-title":"Pinctada fucata mantle gene 3 (PFMG3) promotes differentiation in mouse","volume":"158","author":"Wang","year":"2011","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.jsb.2015.10.012_b0240","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1006\/bbrc.1999.1907","article-title":"Purification and characterization of Perlucin and Perlustrin, two new proteins from the shell of the mollusk Haliotis laevigata","volume":"267","author":"Weiss","year":"2000","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"6679","key":"10.1016\/j.jsb.2015.10.012_b0245","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1038\/31798","article-title":"A marriage of bone and nacre","volume":"392","author":"Westbroek","year":"1998","journal-title":"Nature"},{"issue":"9","key":"10.1016\/j.jsb.2015.10.012_b0250","first-page":"646","article-title":"Burden of major musculoskeletal conditions","volume":"81","author":"Woolf","year":"2003","journal-title":"Bull. World Health Organ."}],"container-title":["Journal of Structural Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1047847715300903?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1047847715300903?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,9,18]],"date-time":"2018-09-18T07:51:07Z","timestamp":1537257067000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1047847715300903"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12]]},"references-count":49,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["S1047847715300903"],"URL":"https:\/\/doi.org\/10.1016\/j.jsb.2015.10.012","relation":{},"ISSN":["1047-8477"],"issn-type":[{"value":"1047-8477","type":"print"}],"subject":[],"published":{"date-parts":[[2015,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Nacre extract restores the mineralization capacity of subchondral osteoarthritis osteoblasts","name":"articletitle","label":"Article Title"},{"value":"Journal of Structural Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jsb.2015.10.012","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}