{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:52:07Z","timestamp":1772121127878,"version":"3.50.1"},"reference-count":23,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Structural Biology"],"published-print":{"date-parts":[[2019,1]]},"DOI":"10.1016\/j.jsb.2018.11.006","type":"journal-article","created":{"date-parts":[[2018,11,17]],"date-time":"2018-11-17T11:44:33Z","timestamp":1542455073000},"page":"78-83","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"title":["Changing times: Fluorescence-lifetime analysis of amyloidogenic SF-IAPP fusion protein"],"prefix":"10.1016","volume":"205","author":[{"given":"Olga I.","family":"Antimonova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitry V.","family":"Lebedev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yana A.","family":"Zabrodskaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia A.","family":"Grudinina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrey L.","family":"Timkovsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward","family":"Ramsay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael M.","family":"Shavlovsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir V.","family":"Egorov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jsb.2018.11.006_b0005","doi-asserted-by":"crossref","DOI":"10.1088\/2050-6120\/1\/1\/015006","article-title":"Initial stages of beta-amyloid A\u03b2 1\u201340 and A\u03b2 1\u201342 oligomerization observed using fluorescence decay and molecular dynamics analyses of tyrosine","volume":"1","author":"Amaro","year":"2013","journal-title":"Methods Appl. Fluoresc."},{"key":"10.1016\/j.jsb.2018.11.006_b0010","doi-asserted-by":"crossref","first-page":"29096","DOI":"10.1038\/srep29096","article-title":"In situ characterization of protein aggregates in human tissues affected by light chain amyloidosis: a FTIR microspectroscopy study","volume":"6","author":"Ami","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jsb.2018.11.006_b0015","first-page":"15","article-title":"[Fusion protein of human amylin with green fluorescent protein \u201cSuperfolder\u201d]","volume":"41","author":"Antimonova","year":"2016","journal-title":"Estestv. i Mat. Nauk. v Sovrem. mire"},{"key":"10.1016\/j.jsb.2018.11.006_b0020","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1002\/elps.200305844","article-title":"Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis","volume":"25","author":"Candiano","year":"2004","journal-title":"Electrophoresis"},{"key":"10.1016\/j.jsb.2018.11.006_b0025","doi-asserted-by":"crossref","first-page":"2156","DOI":"10.1039\/c3an36798c","article-title":"Protein amyloids develop an intrinsic fluorescence signature during aggregation","volume":"138","author":"Chan","year":"2013","journal-title":"Analyst"},{"key":"10.1016\/j.jsb.2018.11.006_b0030","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1070\/PU1982v025n12ABEH005012","article-title":"Solitons in quasi-one-dimensional molecular structures","volume":"25","author":"Davydov","year":"1982","journal-title":"Sov. Phys. Uspekhi"},{"key":"10.1016\/j.jsb.2018.11.006_b0035","article-title":"The toxic nature of murine amylin and the immune responsivity of pancreatic islet to conformational antibody in mice","volume":"1\u20137","author":"Erthal","year":"2018","journal-title":"Mol. Cell. Biochem."},{"key":"10.1016\/j.jsb.2018.11.006_b0040","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1016\/S0002-9440(10)65360-6","article-title":"Intracellular amyloidogenesis by human islet amyloid polypeptide induces apoptosis in COS-1 cells","volume":"154","author":"Hiddinga","year":"1999","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.jsb.2018.11.006_b0045","series-title":"Bio-Nanoimaging. Protein Misfolding and Aggregation","first-page":"105","article-title":"Probing amyloid aggregation and morphology in situ by multiparameter imaging and super-resolution fluorescence microscopy","author":"Kaminski Schierle","year":"2014"},{"key":"10.1016\/j.jsb.2018.11.006_b0050","doi-asserted-by":"crossref","first-page":"477","DOI":"10.5012\/bkcs.2006.27.4.477","article-title":"Fluorescence lifetime imaging microscopy of amyloid aggregates","volume":"27","author":"Koh","year":"2006","journal-title":"Bull. Korean Chem. Soc."},{"key":"10.1016\/j.jsb.2018.11.006_b0055","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.1002\/pmic.200600853","article-title":"Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv","volume":"7","author":"M\u00e5len","year":"2007","journal-title":"Proteomics"},{"key":"10.1016\/j.jsb.2018.11.006_b0060","doi-asserted-by":"crossref","first-page":"181","DOI":"10.2478\/s11534-011-0096-2","article-title":"Gwyddion: an open-source software for SPM data analysis","volume":"10","author":"Ne\u010das","year":"2012","journal-title":"Open Phys."},{"key":"10.1016\/j.jsb.2018.11.006_b0065","doi-asserted-by":"crossref","first-page":"3808","DOI":"10.1021\/acs.biochem.7b00209","article-title":"Role of site-specific asparagine deamidation in islet amyloid polypeptide amyloidogenesis: key contributions of residues 14 and 21","volume":"56","author":"Nguyen","year":"2017","journal-title":"Biochemistry"},{"key":"10.1016\/j.jsb.2018.11.006_b0070","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/nbt1172","article-title":"Engineering and characterization of a superfolder green fluorescent protein","volume":"24","author":"P\u00e9delacq","year":"2006","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.jsb.2018.11.006_b0075","series-title":"Amyloid Proteins. Methods in Molecular Biology (Methods and Protocols)","first-page":"411","article-title":"Histological staining of amyloid and pre-amyloid peptides and proteins in mouse tissue","author":"Rajamohamedsait","year":"2012"},{"key":"10.1016\/j.jsb.2018.11.006_b0080","doi-asserted-by":"crossref","first-page":"221","DOI":"10.3109\/13506129.2014.964858","article-title":"Nomenclature 2014: amyloid fibril proteins and clinical classification of the amyloidosis","volume":"21","author":"Sipe","year":"2014","journal-title":"Amyloid"},{"key":"10.1016\/j.jsb.2018.11.006_b0085","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1080\/10826068.2010.548433","article-title":"Expression in E. coli and purification of the fibrillogenic fusion proteins TTR-sfGFP and \u03b22M-sfGFP","volume":"41","author":"Solovyov","year":"2011","journal-title":"Prep. Biochem. Biotechnol."},{"key":"10.1016\/j.jsb.2018.11.006_b0090","series-title":"Biomarkers and Biological Spectral Imaging","first-page":"92","article-title":"Influence of the refractive index on EGFP fluorescence lifetimes in mixtures of water and glycerol","author":"Suhling","year":"2001"},{"key":"10.1016\/j.jsb.2018.11.006_b0095","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1023\/A:1015323606154","article-title":"The influence of solvent viscosity on the fluorescence decay and time-resolved anisotropy of green fluorescent protein","volume":"12","author":"Suhling","year":"2002","journal-title":"J. Fluoresc."},{"key":"10.1016\/j.jsb.2018.11.006_b0100","doi-asserted-by":"crossref","first-page":"3589","DOI":"10.1016\/S0006-3495(02)75359-9","article-title":"Imaging the environment of green fluorescent protein","volume":"83","author":"Suhling","year":"2002","journal-title":"Biophys. J."},{"key":"10.1016\/j.jsb.2018.11.006_b0105","unstructured":"SymPhoTime 64: Fluorescence Lifetime Imaging and Correlation Software [WWW Document], n.d. Available from: https:\/\/picoquant.com\/products\/category\/software\/symphotime-64-fluorescence-lifetime-imaging-and-correlation-software."},{"key":"10.1016\/j.jsb.2018.11.006_b0110","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1517\/14728222.2012.674111","article-title":"Targeting intracellular and extracellular alpha-synuclein as a therapeutic strategy in Parkinson\u2019s disease and other synucleinopathies","volume":"16","author":"Vekrellis","year":"2012","journal-title":"Expert Opin. Ther. Targets"},{"key":"10.1016\/j.jsb.2018.11.006_b0115","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1039\/b107770h","article-title":"The emission spectrum and the radiative lifetime of Eu3+ in luminescent lanthanide complexes","volume":"4","author":"Werts","year":"2002","journal-title":"Phys. Chem. Chem. Phys."}],"container-title":["Journal of Structural Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1047847718302892?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1047847718302892?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,1,21]],"date-time":"2019-01-21T02:25:55Z","timestamp":1548037555000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1047847718302892"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["S1047847718302892"],"URL":"https:\/\/doi.org\/10.1016\/j.jsb.2018.11.006","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/317917","asserted-by":"object"}]},"ISSN":["1047-8477"],"issn-type":[{"value":"1047-8477","type":"print"}],"subject":[],"published":{"date-parts":[[2019,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Changing times: Fluorescence-lifetime analysis of amyloidogenic SF-IAPP fusion protein","name":"articletitle","label":"Article Title"},{"value":"Journal of Structural Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jsb.2018.11.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}