{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:06:13Z","timestamp":1787022373522,"version":"build-2736575974"},"reference-count":47,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,4,4]],"date-time":"2023-04-04T00:00:00Z","timestamp":1680566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["F31 HL156431"],"award-info":[{"award-number":["F31 HL156431"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 GM073791"],"award-info":[{"award-number":["R01 GM073791"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["S10OD023592"],"award-info":[{"award-number":["S10OD023592"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["HSSN261200800001E"],"award-info":[{"award-number":["HSSN261200800001E"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012001","name":"Al Jalila Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020917","name":"Mohammed Bin Rashid University of Medicine and Health Sciences","doi-asserted-by":"publisher","award":["MBRU-CM-RG2020-14"],"award-info":[{"award-number":["MBRU-CM-RG2020-14"]}],"id":[{"id":"10.13039\/501100020917","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020917","name":"Mohammed Bin Rashid University of Medicine and Health Sciences","doi-asserted-by":"publisher","award":["MBRU-CM-RG2022-09"],"award-info":[{"award-number":["MBRU-CM-RG2022-09"]}],"id":[{"id":"10.13039\/501100020917","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Structural Biology"],"published-print":{"date-parts":[[2023,6]]},"DOI":"10.1016\/j.jsb.2023.107960","type":"journal-article","created":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T17:40:27Z","timestamp":1680716427000},"page":"107960","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["Single particle cryo-EM analysis of Rickettsia conorii Sca2 reveals a formin-like core"],"prefix":"10.1016","volume":"215","author":[{"given":"Peter J.","family":"Carman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grzegorz","family":"Rebowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Dominguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saif S.","family":"Alqassim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"19","key":"10.1016\/j.jsb.2023.107960_b0005","doi-asserted-by":"crossref","first-page":"11606","DOI":"10.3390\/ijms231911606","article-title":"Functional Mimicry of Eukaryotic Actin Assembly by Pathogen Effector Proteins","volume":"23","author":"Alqassim","year":"2022","journal-title":"Int J Mol Sci"},{"issue":"3","key":"10.1016\/j.jsb.2023.107960_b0010","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/j.bpj.2018.12.009","article-title":"Rickettsia Sca2 Recruits Two Actin Subunits for Nucleation but Lacks WH2 Domains","volume":"116","author":"Alqassim","year":"2019","journal-title":"Biophys J"},{"issue":"2","key":"10.1016\/j.jsb.2023.107960_b0015","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1111\/mmi.13031","article-title":"Looks can be deceiving: recent insights into the mechanism of protein secretion by the autotransporter pathway","volume":"97","author":"Bernstein","year":"2015","journal-title":"Mol. Microbiol."},{"issue":"7558","key":"10.1016\/j.jsb.2023.107960_b0020","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1038\/nature14356","article-title":"Structures of actin-like ParM filaments show architecture of plasmid-segregating spindles","volume":"523","author":"Bharat","year":"2015","journal-title":"Nature"},{"issue":"9","key":"10.1016\/j.jsb.2023.107960_b0025","doi-asserted-by":"crossref","first-page":"p. ftv078","DOI":"10.1093\/femspd\/ftv078","article-title":"Bacterial nucleators: actin' on actin","volume":"73","author":"Bugalhao","year":"2015","journal-title":"Pathog Dis"},{"issue":"12","key":"10.1016\/j.jsb.2023.107960_b0030","doi-asserted-by":"crossref","first-page":"5272","DOI":"10.1128\/IAI.00201-09","article-title":"The Sca2 autotransporter protein from Rickettsia conorii is sufficient to mediate adherence to and invasion of cultured mammalian cells","volume":"77","author":"Cardwell","year":"2009","journal-title":"Infect. Immun."},{"issue":"9","key":"10.1016\/j.jsb.2023.107960_b0035","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1111\/j.1462-5822.2012.01815.x","article-title":"Identification and characterization of the mammalian association and actin-nucleating domains in the Rickettsia conorii autotransporter protein, Sca2","volume":"14","author":"Cardwell","year":"2012","journal-title":"Cell. Microbiol."},{"issue":"8","key":"10.1016\/j.jsb.2023.107960_b0040","doi-asserted-by":"crossref","first-page":"p. ftw099","DOI":"10.1093\/femspd\/ftw099","article-title":"Actin-based motility of bacterial pathogens: mechanistic diversity and its impact on virulence","volume":"74","author":"Choe","year":"2016","journal-title":"FEMS Pathogens Dis."},{"key":"10.1016\/j.jsb.2023.107960_b0045","doi-asserted-by":"crossref","first-page":"107","DOI":"10.3389\/fcimb.2016.00107","article-title":"Hijacking Host Cell Highways: Manipulation of the Host Actin Cytoskeleton by Obligate Intracellular Bacterial Pathogens","volume":"6","author":"Colonne","year":"2016","journal-title":"Front Cell Infect Microbiol"},{"issue":"6","key":"10.1016\/j.jsb.2023.107960_b0050","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1007\/s12551-018-0468-6","article-title":"Mechanisms of formin-mediated actin assembly and dynamics","volume":"10","author":"Courtemanche","year":"2018","journal-title":"Biophys Rev"},{"issue":"2","key":"10.1016\/j.jsb.2023.107960_b0055","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1111\/cmi.12399","article-title":"Subversion of the cytoskeleton by intracellular bacteria: lessons from Listeria, Salmonella and Vibrio","volume":"17","author":"de Souza Santos","year":"2015","journal-title":"Cell Microbiol"},{"issue":"1","key":"10.1016\/j.jsb.2023.107960_b0060","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.VMBF-0009-2015","article-title":"Type V Secretion Systems in Bacteria","volume":"4","author":"Fan","year":"2016","journal-title":"Microbiol Spectr"},{"issue":"21","key":"10.1016\/j.jsb.2023.107960_b0065","doi-asserted-by":"crossref","first-page":"4597","DOI":"10.1038\/sj.emboj.7601874","article-title":"Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena\/VASP","volume":"26","author":"Ferron","year":"2007","journal-title":"EMBO J"},{"issue":"6112","key":"10.1016\/j.jsb.2023.107960_b0070","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1126\/science.1229091","article-title":"A bipolar spindle of antiparallel ParM filaments drives bacterial plasmid segregation","volume":"338","author":"Gayathri","year":"2012","journal-title":"Science"},{"issue":"1","key":"10.1016\/j.jsb.2023.107960_b0075","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.jsb.2013.02.010","article-title":"Structure of the ParM filament at 8.5A resolution","volume":"184","author":"Gayathri","year":"2013","journal-title":"J Struct Biol"},{"key":"10.1016\/j.jsb.2023.107960_b0080","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1146\/annurev.biochem.75.103004.142647","article-title":"Mechanism and function of formins in the control of actin assembly","volume":"76","author":"Goode","year":"2007","journal-title":"Annu Rev Biochem"},{"issue":"11","key":"10.1016\/j.jsb.2023.107960_b0085","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1038\/ncb2109","article-title":"Rickettsia Sca2 is a bacterial formin-like mediator of actin-based motility","volume":"12","author":"Haglund","year":"2010","journal-title":"Nat. Cell Biol."},{"issue":"8","key":"10.1016\/j.jsb.2023.107960_b0090","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1111\/j.1462-5822.2004.00402.x","article-title":"A Rickettsia WASP-like protein activates the Arp2\/3 complex and mediates actin-based motility","volume":"6","author":"Jeng","year":"2004","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.jsb.2023.107960_b0095","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1146\/annurev-cellbio-100814-125227","article-title":"How bacteria subvert animal cell structure and function","volume":"32","author":"Jimenez","year":"2016","journal-title":"Annu. Rev. Cell Dev. Biol."},{"issue":"7873","key":"10.1016\/j.jsb.2023.107960_b0100","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"issue":"5","key":"10.1016\/j.jsb.2023.107960_b0105","doi-asserted-by":"crossref","first-page":"2240","DOI":"10.1128\/IAI.00100-10","article-title":"Disruption of the Rickettsia rickettsii Sca2 autotransporter inhibits actin-based motility","volume":"78","author":"Kleba","year":"2010","journal-title":"Infect. Immun."},{"issue":"2","key":"10.1016\/j.jsb.2023.107960_b0110","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.cell.2005.11.038","article-title":"Control of the assembly of ATP-and ADP-actin by formins and profilin","volume":"124","author":"Kovar","year":"2006","journal-title":"Cell"},{"issue":"46","key":"10.1016\/j.jsb.2023.107960_b0115","doi-asserted-by":"crossref","first-page":"eadc9239","DOI":"10.1126\/sciadv.adc9239","article-title":"Pointed-end processive elongation of actin filaments by Vibrio effectors VopF and VopL","volume":"8","author":"Kudryashova","year":"2022","journal-title":"Sci Adv"},{"key":"10.1016\/j.jsb.2023.107960_b0120","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.mib.2016.11.007","article-title":"Actin-based motility and cell-to-cell spread of bacterial pathogens","volume":"35","author":"Lamason","year":"2017","journal-title":"Curr. Opin. Microbiol."},{"issue":"29","key":"10.1016\/j.jsb.2023.107960_b0125","doi-asserted-by":"crossref","first-page":"E2677","DOI":"10.1073\/pnas.1307235110","article-title":"Rickettsia Sca2 has evolved formin-like activity through a different molecular mechanism","volume":"110","author":"Madasu","year":"2013","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"10.1016\/j.jsb.2023.107960_b0130","doi-asserted-by":"crossref","DOI":"10.1093\/femspd\/ftab019","article-title":"The enigmatic biology of rickettsiae: recent advances, open questions and outlook","volume":"79","author":"McGinn","year":"2021","journal-title":"Pathog Dis"},{"key":"10.1016\/j.jsb.2023.107960_b0135","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.3389\/fmicb.2019.01163","article-title":"Type V Secretion Systems: An Overview of Passenger Domain Functions","volume":"10","author":"Meuskens","year":"2019","journal-title":"Front Microbiol"},{"issue":"6","key":"10.1016\/j.jsb.2023.107960_b0140","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1016\/S1097-2765(03)00451-9","article-title":"Bacterial mitosis: ParM of plasmid R1 moves plasmid DNA by an actin-like insertional polymerization mechanism","volume":"12","author":"Moller-Jensen","year":"2003","journal-title":"Mol Cell"},{"key":"10.1016\/j.jsb.2023.107960_b0145","doi-asserted-by":"crossref","first-page":"753","DOI":"10.2217\/fmb-2019-0266","article-title":"Recent research milestones in the pathogenesis of human rickettsioses and opportunities ahead","volume":"15","author":"Narra","year":"2020","journal-title":"Future Microbiol"},{"issue":"7025","key":"10.1016\/j.jsb.2023.107960_b0150","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1038\/nature03251","article-title":"Structural basis of actin filament nucleation and processive capping by a formin homology 2 domain","volume":"433","author":"Otomo","year":"2005","journal-title":"Nature"},{"issue":"5957","key":"10.1016\/j.jsb.2023.107960_b0155","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1126\/science.1175862","article-title":"Actin, a central player in cell shape and movement","volume":"326","author":"Pollard","year":"2009","journal-title":"Science"},{"issue":"3","key":"10.1016\/j.jsb.2023.107960_b0160","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nmeth.4169","article-title":"cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination","volume":"14","author":"Punjani","year":"2017","journal-title":"Nat Methods"},{"issue":"1","key":"10.1016\/j.jsb.2023.107960_b0165","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.cub.2013.11.025","article-title":"Rickettsia actin-based motility occurs in distinct phases mediated by different actin nucleators","volume":"24","author":"Reed","year":"2014","journal-title":"Curr Biol"},{"issue":"2","key":"10.1016\/j.jsb.2023.107960_b0170","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.jsb.2015.08.008","article-title":"CTFFIND4: Fast and accurate defocus estimation from electron micrographs","volume":"192","author":"Rohou","year":"2015","journal-title":"J Struct Biol"},{"issue":"5913","key":"10.1016\/j.jsb.2023.107960_b0175","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1126\/science.1164346","article-title":"Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation","volume":"323","author":"Salje","year":"2009","journal-title":"Science"},{"issue":"10","key":"10.1016\/j.jsb.2023.107960_b0180","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1038\/nrmicro2425","article-title":"The ParMRC system: molecular mechanisms of plasmid segregation by actin-like filaments","volume":"8","author":"Salje","year":"2010","journal-title":"Nat Rev Microbiol"},{"issue":"7173","key":"10.1016\/j.jsb.2023.107960_b0185","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1038\/nature06392","article-title":"Segrosome structure revealed by a complex of ParR with centromere DNA","volume":"450","author":"Schumacher","year":"2007","journal-title":"Nature"},{"key":"10.1016\/j.jsb.2023.107960_b0190","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0076-6879(10)81005-5","article-title":"GraFix: stabilization of fragile macromolecular complexes for single particle cryo-EM","volume":"481","author":"Stark","year":"2010","journal-title":"Methods Enzymol"},{"issue":"22","key":"10.1016\/j.jsb.2023.107960_b0195","doi-asserted-by":"crossref","first-page":"3658","DOI":"10.1002\/1873-3468.13173","article-title":"Actin dynamics in host-pathogen interaction","volume":"592","author":"Stradal","year":"2018","journal-title":"FEBS Lett"},{"issue":"1","key":"10.1016\/j.jsb.2023.107960_b0200","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1038\/nsmb.2462","article-title":"FMNL3 FH2-actin structure gives insight into formin-mediated actin nucleation and elongation","volume":"20","author":"Thompson","year":"2013","journal-title":"Nat Struct Mol Biol"},{"issue":"8","key":"10.1016\/j.jsb.2023.107960_b0205","doi-asserted-by":"crossref","first-page":"4706","DOI":"10.1128\/IAI.68.8.4706-4713.2000","article-title":"Ultrastructure of Rickettsia rickettsii actin tails and localization of cytoskeletal proteins","volume":"68","author":"Van Kirk","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.jsb.2023.107960_b0210","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1038\/s42003-019-0437-z","article-title":"SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM","volume":"2","author":"Wagner","year":"2019","journal-title":"Commun Biol"},{"issue":"5","key":"10.1016\/j.jsb.2023.107960_b0215","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/S0092-8674(04)00210-7","article-title":"Crystal structures of a Formin Homology-2 domain reveal a tethered dimer architecture","volume":"116","author":"Xu","year":"2004","journal-title":"Cell"},{"issue":"11","key":"10.1016\/j.jsb.2023.107960_b0220","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1111\/j.1365-2443.2007.01132.x","article-title":"Crystal structure of human DAAM1 formin homology 2 domain","volume":"12","author":"Yamashita","year":"2007","journal-title":"Genes Cells"},{"issue":"4","key":"10.1016\/j.jsb.2023.107960_b0225","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/nmeth.4193","article-title":"MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy","volume":"14","author":"Zheng","year":"2017","journal-title":"Nat Methods"},{"issue":"24","key":"10.1016\/j.jsb.2023.107960_b0230","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmb.2020.10.022","article-title":"Profilin's Affinity for Formin Regulates the Availability of Filament Ends for Actin Monomer Binding","volume":"432","author":"Zweifel","year":"2020","journal-title":"J Mol Biol"},{"issue":"14","key":"10.1016\/j.jsb.2023.107960_b0235","doi-asserted-by":"crossref","first-page":"4513","DOI":"10.1074\/jbc.RA119.012000","article-title":"Competition for delivery of profilin-actin to barbed ends limits the rate of formin-mediated actin filament elongation","volume":"295","author":"Zweifel","year":"2020","journal-title":"J Biol Chem"}],"container-title":["Journal of Structural Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1047847723000230?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1047847723000230?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T03:22:30Z","timestamp":1760930550000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1047847723000230"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":47,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["S1047847723000230"],"URL":"https:\/\/doi.org\/10.1016\/j.jsb.2023.107960","relation":{},"ISSN":["1047-8477"],"issn-type":[{"value":"1047-8477","type":"print"}],"subject":[],"published":{"date-parts":[[2023,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Single particle cryo-EM analysis of Rickettsia conorii Sca2 reveals a formin-like core","name":"articletitle","label":"Article Title"},{"value":"Journal of Structural Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jsb.2023.107960","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"107960"}}