{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:01:08Z","timestamp":1790874068623,"version":"4.1.0"},"reference-count":35,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008454","name":"Boehringer Ingelheim Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008454","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GRK2887"],"award-info":[{"award-number":["GRK2887"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Projektnummer 497350882"],"award-info":[{"award-number":["Projektnummer 497350882"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Structural Biology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.jsb.2026.108378","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T22:52:38Z","timestamp":1790463158000},"page":"108378","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cryo-EM structure of human TMEM45A with a bound 2-Hydroxypalmitate"],"prefix":"10.1016","volume":"218","author":[{"given":"Mariana","family":"Grieben","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julica","family":"Inderhees","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niklas","family":"Ebersberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jsb.2026.108378_bb0005","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: a comprehensive Python-based system for macromolecular structure solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Crystallogr. Sect. D Biol. Crystallogr."},{"key":"10.1016\/j.jsb.2026.108378_bb0010","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1107\/S2059798318006551","article-title":"Real-space refinement in PHENIX for cryo-EM and crystallography","volume":"74","author":"Afonine","year":"2018","journal-title":"Acta Crystallogr. D Struct. Biol."},{"key":"10.1016\/j.jsb.2026.108378_bb0015","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-65601-0","article-title":"CYP17A1 deficient XY mice display susceptibility to atherosclerosis, altered lipidomic profile and atypical sex development","volume":"10","author":"Aherrahrou","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jsb.2026.108378_bb0020","doi-asserted-by":"crossref","first-page":"4587","DOI":"10.1093\/nar\/gkp425","article-title":"An integrative genomics approach identifies hypoxia inducible Factor-1 (HIF-1)-target genes that form the core response to hypoxia","volume":"37","author":"Benita","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jsb.2026.108378_bb0025","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","article-title":"Features and development of coot","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.jsb.2026.108378_bb0030","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2407-12-391","article-title":"TMEM45A is essential for hypoxia-induced chemoresistance in breast and liver cancer cells","volume":"12","author":"Flamant","year":"2012","journal-title":"BMC Cancer"},{"key":"10.1016\/j.jsb.2026.108378_bb0035","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0063949","article-title":"Systematic identification and characterization of novel human skin-associated genes encoding membrane and secreted proteins","volume":"8","author":"Gerber","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.jsb.2026.108378_bb0040","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1083\/jcb.97.4.1303","article-title":"Golgi membranes contain an electrogenic H+ pump in parallel to a chloride conductance","volume":"97","author":"Glickman","year":"1983","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.jsb.2026.108378_bb0045","doi-asserted-by":"crossref","first-page":"108376","DOI":"10.1016\/j.jsb.2026.108376","article-title":"Cryo-EM structure of human TMEM45B with a bound GM3 (18:1;O2\/24:1)","volume":"218","author":"Grieben","year":"2026","journal-title":"Journal of Structural Biology"},{"key":"10.1016\/j.jsb.2026.108378_bb0050","doi-asserted-by":"crossref","first-page":"3124","DOI":"10.3892\/or.2015.3902","article-title":"Inhibition of TMEM45A suppresses proliferation, induces cell cycle arrest and reduces cell invasion in human ovarian cancer cells","volume":"33","author":"Guo","year":"2015","journal-title":"Oncol. Rep."},{"key":"10.1016\/j.jsb.2026.108378_bb0055","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1046\/j.1523-1747.2002.01836.x","article-title":"Human epidermal glucosylceramides are major precursors of stratum corneum ceramides","volume":"119","author":"Hamanaka","year":"2002","journal-title":"J. Invest. Dermatol."},{"key":"10.1016\/j.jsb.2026.108378_bb0060","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1111\/exd.12403","article-title":"High TMEM45A expression is correlated to epidermal keratinization","volume":"23","author":"Hayez","year":"2014","journal-title":"Exp. Dermatol."},{"key":"10.1016\/j.jsb.2026.108378_bb0065","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.jsb.2026.108378_bb0070","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1172\/JCI124159","article-title":"DEGS1-associated aberrant sphingolipid metabolism impairs nervous system function in humans","volume":"129","author":"Karsai","year":"2019","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.jsb.2026.108378_bb0075","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1038\/nmeth.2551","article-title":"LipidBlast in silico tandem mass spectrometry database for lipid identification","volume":"10","author":"Kind","year":"2013","journal-title":"Nat. Methods"},{"key":"10.1016\/j.jsb.2026.108378_bb0080","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1007\/s10555-008-9144-9","article-title":"Clinical biomarkers for hypoxia targeting","volume":"27","author":"Le","year":"2008","journal-title":"Cancer Metastasis Rev."},{"key":"10.1016\/j.jsb.2026.108378_bb0085","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1038\/s41580-020-0227-y","article-title":"Cellular adaptation to hypoxia through hypoxia inducible factors and beyond","volume":"21","author":"Lee","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.jsb.2026.108378_bb0090","article-title":"Golgi metal ion homeostasis in human health and diseases","volume":"11","author":"Li","year":"2022","journal-title":"Cells"},{"key":"10.1016\/j.jsb.2026.108378_bb0095","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1007\/978-3-030-23173-6_19","article-title":"Golgi structure and function in health, stress, and diseases","volume":"67","author":"Li","year":"2019","journal-title":"Results Probl. Cell Differ."},{"key":"10.1016\/j.jsb.2026.108378_bb0100","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1107\/S2059798319011471","article-title":"Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix","volume":"75","author":"Liebschner","year":"2019","journal-title":"Acta Crystallogr. D Struct. Biol."},{"key":"10.1016\/j.jsb.2026.108378_bb0105","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.semcancer.2019.08.018","article-title":"Transmembrane (TMEM) protein family members: poorly characterized even if essential for the metastatic process","volume":"60","author":"Marx","year":"2020","journal-title":"Semin. Cancer Biol."},{"key":"10.1016\/j.jsb.2026.108378_bb0110","doi-asserted-by":"crossref","DOI":"10.1002\/pro.4792","article-title":"UCSF ChimeraX: tools for structure building and analysis","volume":"32","author":"Meng","year":"2023","journal-title":"Protein Sci."},{"key":"10.1016\/j.jsb.2026.108378_bb0115","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-019-44204-4","article-title":"Human skin equivalents cultured under hypoxia display enhanced epidermal morphogenesis and lipid barrier formation","volume":"9","author":"Mieremet","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jsb.2026.108378_bb0120","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1038\/s41592-022-01488-1","article-title":"ColabFold: making protein folding accessible to all","volume":"19","author":"Mirdita","year":"2022","journal-title":"Nat. Methods"},{"key":"10.1016\/j.jsb.2026.108378_bb0125","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1107\/S0907444909029436","article-title":"Electronic ligand builder and optimization workbench (eLBOW): a tool for ligand coordinate and restraint generation","volume":"65","author":"Moriarty","year":"2009","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.jsb.2026.108378_bb0130","doi-asserted-by":"crossref","first-page":"83","DOI":"10.2147\/HP.S93413","article-title":"The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy","volume":"3","author":"Muz","year":"2015","journal-title":"Hypoxia (Auckl)"},{"key":"10.1016\/j.jsb.2026.108378_bb0135","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF chimera--a visualization system for exploratory research and analysis","volume":"25","author":"Pettersen","year":"2004","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.jsb.2026.108378_bb0140","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nmeth.4169","article-title":"cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination","volume":"14","author":"Punjani","year":"2017","journal-title":"Nat. Methods"},{"key":"10.1016\/j.jsb.2026.108378_bb0145","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1038\/s41592-020-00990-8","article-title":"Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction","volume":"17","author":"Punjani","year":"2020","journal-title":"Nat. Methods"},{"key":"10.1016\/j.jsb.2026.108378_bb0150","doi-asserted-by":"crossref","DOI":"10.1038\/s41419-019-2088-x","article-title":"Characterization of the role of TMEM45A in cancer cell sensitivity to cisplatin","volume":"10","author":"Schmit","year":"2019","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.jsb.2026.108378_bb0155","first-page":"12657","article-title":"Knockdown of TMEM45A inhibits the proliferation, migration and invasion of glioma cells","volume":"8","author":"Sun","year":"2015","journal-title":"Int. J. Clin. Exp. Pathol."},{"key":"10.1016\/j.jsb.2026.108378_bb0160","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s10969-013-9157-7","article-title":"Finding non-crystallographic symmetry in density maps of macromolecular structures","volume":"14","author":"Terwilliger","year":"2013","journal-title":"J. Struct. Funct. Genom."},{"key":"10.1016\/j.jsb.2026.108378_bb0165","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1002\/pro.3330","article-title":"MolProbity: more and better reference data for improved all-atom structure validation","volume":"27","author":"Williams","year":"2018","journal-title":"Protein Sci."},{"key":"10.1016\/j.jsb.2026.108378_bb0170","article-title":"Dysregulated ceramides metabolism by fatty acid 2-hydroxylase exposes a metabolic vulnerability to target cancer metastasis","volume":"7","author":"Zhou","year":"2022","journal-title":"Signal Transduct. Target. Ther."},{"key":"10.1016\/j.jsb.2026.108378_bb0175","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1111\/1440-1681.13220","article-title":"Knockdown of TMEM45A overcomes multidrug resistance and epithelial-mesenchymal transition in human colorectal cancer cells through inhibition of TGF-\u03b2 signalling pathway","volume":"47","author":"Zhu","year":"2020","journal-title":"Clin. Exp. Pharmacol. Physiol."}],"container-title":["Journal of Structural Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1047847726000948?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1047847726000948?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:13:36Z","timestamp":1790871216000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1047847726000948"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":35,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["S1047847726000948"],"URL":"https:\/\/doi.org\/10.1016\/j.jsb.2026.108378","relation":{},"ISSN":["1047-8477"],"issn-type":[{"value":"1047-8477","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cryo-EM structure of human TMEM45A with a bound 2-Hydroxypalmitate","name":"articletitle","label":"Article Title"},{"value":"Journal of Structural Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jsb.2026.108378","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"108378"}}