{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:23:47Z","timestamp":1789777427596,"version":"4.0.1"},"reference-count":37,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["jthjournal.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Thrombosis and Haemostasis"],"published-print":{"date-parts":[[2024,10]]},"DOI":"10.1016\/j.jtha.2024.05.037","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T03:15:32Z","timestamp":1719285332000},"page":"2910-2921","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"title":["Comparison of assays measuring extracellular vesicle tissue factor in plasma samples: communication from the ISTH SSC Subcommittee on Vascular Biology"],"prefix":"10.1016","volume":"22","author":[{"given":"Amandine","family":"Bonifay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigel","family":"Mackman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohei","family":"Hisada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Teresa Azevedo","family":"Sachetto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi","family":"Hau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine","family":"Gray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Hogwood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anat","family":"Aharon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Badimon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucio","family":"Barile","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justine","family":"Baudar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lennart","family":"Beckmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birke","family":"Benedikter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Bolis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarik","family":"Bouriche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Brambilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacopo","family":"Burrello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Camera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Campello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Ettelaie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doroth\u00e9e","family":"Faille","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Featherby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corentin","family":"Franco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maite","family":"Guldenpfennig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John-Bjarne","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coralie","family":"Judicone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohan","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soren Risom","family":"Kristensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Laakmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Langer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadezhda","family":"Latysheva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Lucien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika Marques","family":"de Menezes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Mullier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Norris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jette","family":"Nybo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josune","family":"Orbe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bjarne","family":"Osterud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose A.","family":"Paramo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia M.","family":"Radu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Roncal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazanin","family":"Samadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omri","family":"Snir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Suades","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Casper","family":"Wahlund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Chareyre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyne","family":"Abdili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1026-6107","authenticated-orcid":false,"given":"Kimberly","family":"Martinod","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Thaler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Dignat-George","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rienk","family":"Nieuwland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romaric","family":"Lacroix","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jtha.2024.05.037_bib1","doi-asserted-by":"crossref","first-page":"2309","DOI":"10.7150\/thno.39486","article-title":"Extracellular vesicles: the next generation of biomarkers for liquid biopsy-based prostate cancer diagnosis","volume":"10","author":"Pang","year":"2020","journal-title":"Theranostics"},{"key":"10.1016\/j.jtha.2024.05.037_bib2","doi-asserted-by":"crossref","first-page":"3172","DOI":"10.1182\/blood-2010-06-290460","article-title":"Cell-derived vesicles exposing coagulant tissue factor in saliva","volume":"117","author":"Berckmans","year":"2011","journal-title":"Blood"},{"key":"10.1016\/j.jtha.2024.05.037_bib3","doi-asserted-by":"crossref","first-page":"3357","DOI":"10.18632\/oncotarget.6581","article-title":"Detection of EpCAM-positive microparticles in pleural fluid: a new approach to mini-invasively identify patients with malignant pleural effusions","volume":"7","author":"Roca","year":"2016","journal-title":"Oncotarget"},{"key":"10.1016\/j.jtha.2024.05.037_bib4","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1161\/ATVBAHA.117.309846","article-title":"Tissue factor: an essential mediator of hemostasis and trigger of thrombosis","volume":"38","author":"Grover","year":"2018","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"10.1016\/j.jtha.2024.05.037_bib5","doi-asserted-by":"crossref","first-page":"e574","DOI":"10.1097\/CCM.0000000000001584","article-title":"Microvesicle tissue factor activity and interleukin-8 levels are associated with mortality in patients with influenza A\/H1N1 infection","volume":"44","author":"Rondina","year":"2016","journal-title":"Crit Care Med"},{"key":"10.1016\/j.jtha.2024.05.037_bib6","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1055\/s-0039-1693476","article-title":"Microvesicles and cancer associated thrombosis","volume":"45","author":"Lacroix","year":"2019","journal-title":"Semin Thromb Hemost"},{"key":"10.1016\/j.jtha.2024.05.037_bib7","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1182\/bloodadvances.2020003308","article-title":"Dissemination of extreme levels of extracellular vesicles: tissue factor activity in patients with severe COVID-19","volume":"5","author":"Guervilly","year":"2021","journal-title":"Blood Adv"},{"key":"10.1016\/j.jtha.2024.05.037_bib8","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1161\/ATVBAHA.120.315547","article-title":"Patients with COVID-19 have elevated levels of circulating extracellular vesicle tissue factor activity that is associated with severity and mortality\u2013brief report","volume":"41","author":"Rosell","year":"2021","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"10.1016\/j.jtha.2024.05.037_bib9","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1097\/MOH.0000000000000730","article-title":"Measurement of tissue factor-positive extracellular vesicles in plasma: strengths and weaknesses of current methods","volume":"29","author":"Mackman","year":"2022","journal-title":"Curr Opin Hematol"},{"key":"10.1016\/j.jtha.2024.05.037_bib10","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1111\/jth.15606","article-title":"A\u00a0rapid, sensitive, and specific assay to measure TF activity based on chromogenic determination of thrombin generation","volume":"20","author":"\u00d8sterud","year":"2022","journal-title":"J\u00a0Thromb Haemost"},{"key":"10.1016\/j.jtha.2024.05.037_bib11","doi-asserted-by":"crossref","first-page":"6274","DOI":"10.1182\/bloodadvances.2020003012","article-title":"Human milk triggers coagulation via tissue factor-exposing extracellular vesicles","volume":"4","author":"Hu","year":"2020","journal-title":"Blood Adv"},{"key":"10.1016\/j.jtha.2024.05.037_bib12","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1055\/a-2091-7006","article-title":"Monocyte tissue factor expression: lipopolysaccharide induction and roles in pathological activation of coagulation","volume":"123","author":"Sachetto","year":"2023","journal-title":"Thromb Haemost"},{"key":"10.1016\/j.jtha.2024.05.037_bib13","article-title":"Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines","volume":"7","author":"Th\u00e9ry","year":"2018","journal-title":"J\u00a0Extracell Vesicles"},{"key":"10.1016\/j.jtha.2024.05.037_bib14","article-title":"A\u00a0new strategy to count and sort neutrophil-derived extracellular vesicles: validation in infectious disorders","volume":"11","author":"Bonifay","year":"2022","journal-title":"J\u00a0Extracell Vesicles"},{"key":"10.1016\/j.jtha.2024.05.037_bib15","article-title":"Single-step isolation of extracellular vesicles by size-exclusion chromatography","volume":"3","author":"B\u00f6ing","year":"2014","journal-title":"J\u00a0Extracell Vesicles"},{"key":"10.1016\/j.jtha.2024.05.037_bib16","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1002\/rth2.12165","article-title":"Measurement of tissue factor activity in extracellular vesicles from human plasma samples","volume":"3","author":"Hisada","year":"2018","journal-title":"Res Pract Thromb Haemost"},{"key":"10.1016\/j.jtha.2024.05.037_bib17","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1111\/j.1538-7836.2011.04610.x","article-title":"Impact of pre-analytical parameters on the measurement of circulating microparticles: towards standardization of protocol","volume":"10","author":"Lacroix","year":"2012","journal-title":"J\u00a0Thromb Haemost"},{"key":"10.1016\/j.jtha.2024.05.037_bib18","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1111\/jth.14481","article-title":"Toward standardization of assays measuring extracellular vesicle-associated tissue factor activity","volume":"17","author":"Nieuwland","year":"2019","journal-title":"J\u00a0Thromb Haemost"},{"key":"10.1016\/j.jtha.2024.05.037_bib19","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1097\/MBC.0b013e328354a256","article-title":"Microparticle-associated tissue factor activity measured with the Zymuphen MP-TF kit and the calibrated automated thrombogram assay","volume":"23","author":"Hellum","year":"2012","journal-title":"Blood Coagul Fibrinolysis"},{"key":"10.1016\/j.jtha.2024.05.037_bib20","doi-asserted-by":"crossref","first-page":"1932","DOI":"10.1111\/jth.12718","volume":"12","author":"Tatsumi","year":"2014","journal-title":"J\u00a0Thromb Haemost"},{"key":"10.1016\/j.jtha.2024.05.037_bib21","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.thromres.2019.07.011","article-title":"Increasing the sensitivity of the human microvesicle tissue factor activity assay","volume":"182","author":"Vallier","year":"2019","journal-title":"Thromb Res"},{"key":"10.1016\/j.jtha.2024.05.037_bib22","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1016\/j.jtha.2022.12.026","article-title":"\u201cA rapid, sensitive, and specific assay to measure tissue factor activity based on chromogenic determination of thrombin generation\u201d: comment from Tanratana et\u00a0al","volume":"21","author":"Tanratana","year":"2023","journal-title":"J\u00a0Thromb Haemost"},{"key":"10.1016\/j.jtha.2024.05.037_bib23","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1111\/jth.13181","article-title":"Tissue factor-positive tumor microvesicles activate platelets and enhance thrombosis in mice","volume":"14","author":"Geddings","year":"2016","journal-title":"J\u00a0Thromb Haemost"},{"key":"10.1016\/j.jtha.2024.05.037_bib24","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1042\/bj2650327","article-title":"The contributions of Ca2+, phospholipids and tissue-factor apoprotein to the activation of human blood-coagulation factor X by activated factor VII","volume":"265","author":"Bom","year":"1990","journal-title":"Biochem J"},{"key":"10.1016\/j.jtha.2024.05.037_bib25","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.1249\/MSS.0000000000001985","article-title":"Microparticle responses to aerobic exercise and meal consumption in healthy men","volume":"51","author":"Highton","year":"2019","journal-title":"Med Sci Sports Exerc"},{"key":"10.1016\/j.jtha.2024.05.037_bib26","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1111\/dom.13548","article-title":"Effects of acute insulin-induced hypoglycaemia on endothelial microparticles in adults with\u00a0and without type 2 diabetes","volume":"21","author":"Al-Qaissi","year":"2019","journal-title":"Diabetes Obes Metab"},{"key":"10.1016\/j.jtha.2024.05.037_bib27","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1160\/TH16-02-0130","article-title":"CD142+\/CD61+, CD146+ and CD45+ microparticles predict cardiovascular events in high risk patients following a Mediterranean diet supplemented with nuts","volume":"116","author":"Chiva-Blanch","year":"2016","journal-title":"Thromb Haemost"},{"key":"10.1016\/j.jtha.2024.05.037_bib28","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.thromres.2017.04.019","article-title":"Circulating microvesicle number, function and small RNA content vary with age, gender, smoking status, lipid and hormone profiles","volume":"156","author":"Enjeti","year":"2017","journal-title":"Thromb Res"},{"key":"10.1016\/j.jtha.2024.05.037_bib29","doi-asserted-by":"crossref","first-page":"97394","DOI":"10.18632\/oncotarget.22128","article-title":"Fibrin-bearing microparticles: marker of thrombo-embolic events in pancreatic and colorectal cancers","volume":"8","author":"Mege","year":"2017","journal-title":"Oncotarget"},{"key":"10.1016\/j.jtha.2024.05.037_bib30","article-title":"MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments","volume":"9","author":"Welsh","year":"2020","journal-title":"J\u00a0Extracell Vesicles"},{"key":"10.1016\/j.jtha.2024.05.037_bib31","article-title":"A\u00a0compendium of single extracellular vesicle flow cytometry","volume":"12","author":"Welsh","year":"2023","journal-title":"J\u00a0Extracell Vesicles"},{"key":"10.1016\/j.jtha.2024.05.037_bib32","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.thromres.2011.01.002","article-title":"Endothelial, platelet, and tissue factor-bearing microparticles in cancer patients with and without venous thromboembolism","volume":"127","author":"Campello","year":"2011","journal-title":"Thromb Res"},{"key":"10.1016\/j.jtha.2024.05.037_bib33","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.cca.2022.09.011","article-title":"Annexin V- and tissue factor+ microparticles as biomarkers for predicting deep vein thrombosis in patients after joint arthroplasty","volume":"536","author":"Chen","year":"2022","journal-title":"Clin Chim Acta"},{"key":"10.1016\/j.jtha.2024.05.037_bib34","doi-asserted-by":"crossref","DOI":"10.1002\/rth2.12677","article-title":"Evaluation of a new bead-based assay to measure levels of human tissue factor antigen in extracellular vesicles in plasma","volume":"6","author":"Archibald","year":"2022","journal-title":"Res Pract Thromb Haemost"},{"key":"10.1016\/j.jtha.2024.05.037_bib35","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s12959-016-0123-z","article-title":"Pre-analytical issues in the haemostasis laboratory: guidance for the clinical laboratories","volume":"14","author":"Magnette","year":"2016","journal-title":"Thromb J"},{"key":"10.1016\/j.jtha.2024.05.037_bib36","article-title":"The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles","volume":"3","author":"Cvjetkovic","year":"2014","journal-title":"J\u00a0Extracell Vesicles"},{"key":"10.1016\/j.jtha.2024.05.037_bib37","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.thromres.2020.09.020","article-title":"A\u00a0new hybrid immunocapture bioassay with improved reproducibility to measure tissue factor-dependent procoagulant activity of microvesicles from body fluids","volume":"196","author":"Franco","year":"2020","journal-title":"Thromb Res"}],"container-title":["Journal of Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1538783624003672?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1538783624003672?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T20:46:33Z","timestamp":1759351593000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1538783624003672"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":37,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["S1538783624003672"],"URL":"https:\/\/doi.org\/10.1016\/j.jtha.2024.05.037","relation":{},"ISSN":["1538-7836"],"issn-type":[{"value":"1538-7836","type":"print"}],"subject":[],"published":{"date-parts":[[2024,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comparison of assays measuring extracellular vesicle tissue factor in plasma samples: communication from the ISTH SSC Subcommittee on Vascular Biology","name":"articletitle","label":"Article Title"},{"value":"Journal of Thrombosis and Haemostasis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jtha.2024.05.037","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Published by Elsevier Inc. on behalf of International Society on Thrombosis and Haemostasis.","name":"copyright","label":"Copyright"}]}}