{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T13:03:16Z","timestamp":1780578196875,"version":"3.54.1"},"reference-count":49,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Dutch Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jthjournal.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Thrombosis and Haemostasis"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.jtha.2026.02.015","type":"journal-article","created":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T16:26:07Z","timestamp":1772641567000},"page":"2071-2086","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Advancing precision diagnostics in coagulation: introduction and analytical validation of a multiplex mass spectrometry\u2013based test for 5 coagulation factors"],"prefix":"10.1016","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8819-3446","authenticated-orcid":false,"given":"Noa A.","family":"Linthorst","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard J.","family":"Dirven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred P.H.T.M.","family":"Romijn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rama","family":"Mutlaq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Cordfunke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natasja","family":"Dolezal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan W.","family":"Drijfhout","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mettine H.A.","family":"Bos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen C.J.","family":"Eikenboom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christa M.","family":"Cobbaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart J.M.","family":"van Vlijmen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. Renee","family":"Ruhaak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jtha.2026.02.015_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.thromres.2024.109090","article-title":"Quantitative protein mass spectrometry for multiplex measurement of coagulation and fibrinolytic proteins towards clinical application: what, why and how?","volume":"241","author":"Camilleri","year":"2024","journal-title":"Thromb Res"},{"key":"10.1016\/j.jtha.2026.02.015_bib2","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1055\/s-2001-16885","article-title":"Molecular biology of blood coagulation","volume":"27","author":"Oldenburg","year":"2001","journal-title":"Semin Thromb Hemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib3","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1111\/j.1538-7836.2008.02981.x","article-title":"D-dimer for venous thromboembolism diagnosis: 20 years later","volume":"6","author":"Righini","year":"2008","journal-title":"J Thromb Haemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib4","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.thromres.2024.04.018","article-title":"D-dimer and risk of venous thromboembolism recurrence: comparison of two studies with similar designs but different laboratory and clinical results","volume":"238","author":"Palareti","year":"2024","journal-title":"Thromb Res"},{"key":"10.1016\/j.jtha.2026.02.015_bib5","first-page":"41","article-title":"Perils, problems, and progress in laboratory diagnosis of von Willebrand disease","volume":"40","author":"Flood","year":"2014","journal-title":"Semin Thromb Hemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib6","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1111\/jth.14784","article-title":"Laboratory testing in hemophilia: impact of factor and non-factor replacement therapy on coagulation assays","volume":"18","author":"Peyvandi","year":"2020","journal-title":"J Thromb Haemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib7","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1055\/s-0042-1758870","article-title":"Factor VIII and factor IX activity measurements for hemophilia diagnosis and related treatments","volume":"49","author":"Bowyer","year":"2023","journal-title":"Semin Thromb Hemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib8","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1055\/s-0044-1792033","article-title":"Laboratory and molecular diagnosis of factor XI deficiency","volume":"51","author":"Davidson","year":"2025","journal-title":"Semin Thromb Hemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib9","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/978-1-4939-7196-1_2","article-title":"Preanalytical issues in hemostasis and thrombosis testing","volume":"1646","author":"Lippi","year":"2017","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.jtha.2026.02.015_bib10","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s12959-016-0123-z","article-title":"Pre-analytical issues in the haemostasis laboratory: guidance for the clinical laboratories","volume":"14","author":"Magnette","year":"2016","journal-title":"Thromb J"},{"key":"10.1016\/j.jtha.2026.02.015_bib11","doi-asserted-by":"crossref","first-page":"864","DOI":"10.4065\/82.7.864","article-title":"How to interpret and pursue an abnormal prothrombin time, activated partial thromboplastin time, and bleeding time in adults","volume":"82","author":"Kamal","year":"2007","journal-title":"Mayo Clin Proc"},{"key":"10.1016\/j.jtha.2026.02.015_bib12","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.1111\/jth.13368","article-title":"A diagnostic approach to mild bleeding disorders","volume":"14","author":"Boender","year":"2016","journal-title":"J Thromb Haemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib13","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/jth.14363","article-title":"Diagnostic challenges of inherited mild bleeding disorders: a bait for poorly explored clinical and basic research","volume":"17","author":"Mezzano","year":"2019","journal-title":"J Thromb Haemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib14","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/jth.14648","article-title":"Recommendations for clinical laboratory testing for antithrombin deficiency; Communication from the SSC of the ISTH","volume":"18","author":"Van Cott","year":"2020","journal-title":"J Thromb Haemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib15","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/jth.14667","article-title":"Recommendations for clinical laboratory testing for protein C deficiency, for the subcommittee on plasma coagulation inhibitors of the ISTH","volume":"18","author":"Cooper","year":"2020","journal-title":"J Thromb Haemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib16","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1182\/bloodadvances.2020003265","article-title":"ASH ISTH NHF WFH 2021 guidelines on the diagnosis of von Willebrand disease","volume":"5","author":"James","year":"2021","journal-title":"Blood Adv"},{"key":"10.1016\/j.jtha.2026.02.015_bib17","doi-asserted-by":"crossref","DOI":"10.1183\/13993003.01647-2019","volume":"54","author":"Konstantinides","year":"2019","journal-title":"Eur Respir J"},{"key":"10.1016\/j.jtha.2026.02.015_bib18","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1080\/14789450.2016.1253477","article-title":"Prospective applications of ultrahigh resolution proteomics in clinical mass spectrometry","volume":"13","author":"Ruhaak","year":"2016","journal-title":"Expert Rev Proteom"},{"key":"10.1016\/j.jtha.2026.02.015_bib19","doi-asserted-by":"crossref","DOI":"10.1002\/rth2.12706","article-title":"Proteomics in thrombosis research","volume":"6","author":"Edfors","year":"2022","journal-title":"Res Pract Thromb Haemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib20","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1021\/jasms.0c00379","article-title":"The time has come for quantitative protein mass spectrometry tests that target unmet clinical needs","volume":"32","author":"Smit","year":"2021","journal-title":"J Am Soc Mass Spectrom"},{"key":"10.1016\/j.jtha.2026.02.015_bib21","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/978-1-0716-1024-4_8","article-title":"Quantitative mass spectrometry-based proteomics: an overview","volume":"2228","author":"Rozanova","year":"2021","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.jtha.2026.02.015_bib22","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/978-1-62703-405-0_13","article-title":"Absolute quantitation of proteins in human blood by multiplexed multiple reaction monitoring mass spectrometry","volume":"1000","author":"Percy","year":"2013","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.jtha.2026.02.015_bib23","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1074\/mcp.M113.036095","article-title":"Targeted peptide measurements in biology and medicine: best practices for mass spectrometry-based assay development using a fit-for-purpose approach","volume":"13","author":"Carr","year":"2014","journal-title":"Mol Cell Proteomics"},{"key":"10.1016\/j.jtha.2026.02.015_bib24","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.jim.2009.06.003","article-title":"The fundamental flaws of immunoassays and potential solutions using tandem mass spectrometry","volume":"347","author":"Hoofnagle","year":"2009","journal-title":"J Immunol Methods"},{"key":"10.1016\/j.jtha.2026.02.015_bib25","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1182\/bloodadvances.2017007955","article-title":"Multiplexed targeted proteomic assay to assess coagulation factor concentrations and thrombosis-associated cancer","volume":"1","author":"Mohammed","year":"2017","journal-title":"Blood Adv"},{"key":"10.1016\/j.jtha.2026.02.015_bib26","unstructured":"International Organization for Standardization. ISO 15189:2022. Medical laboratories \u2013 requirements for quality and competence. 2022. https:\/\/www.iso.org\/standard\/76677.html. [accessed February 11, 2026]."},{"key":"10.1016\/j.jtha.2026.02.015_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.rpth.2023.100079","article-title":"Antithrombin diagnostics by mass spectrometry: development and analytical validation of a next-generation test","volume":"7","author":"Kruijt","year":"2023","journal-title":"Res Pract Thromb Haemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib28","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.jtha.2024.10.005","article-title":"Novel insights into antithrombin deficiency enabled by mass spectrometry-based precision diagnostics","volume":"23","author":"Kruijt","year":"2024","journal-title":"J Thromb Haemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib29","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1373\/clinchem.2015.246702","article-title":"Automated multiplex LC-MS\/MS assay for quantifying serum apolipoproteins A-I, B, C-I, C-II, C-III, and E with qualitative apolipoprotein E phenotyping","volume":"62","author":"van den Broek","year":"2016","journal-title":"Clin Chem"},{"key":"10.1016\/j.jtha.2026.02.015_bib30","doi-asserted-by":"crossref","first-page":"1704","DOI":"10.1515\/cclm-2017-1111","article-title":"Detecting molecular forms of antithrombin by LC-MRM-MS: defining the measurands","volume":"56","author":"Ruhaak","year":"2018","journal-title":"Clin Chem Lab Med"},{"key":"10.1016\/j.jtha.2026.02.015_bib31","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/jth.15553","article-title":"Unraveling a borderline antithrombin deficiency case with quantitative mass spectrometry","volume":"20","author":"Kruijt","year":"2022","journal-title":"J Thromb Haemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib32","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1111\/jth.15623","article-title":"Targeted proteomics for evaluating risk of venous thrombosis following traumatic lower-leg injury or knee arthroscopy","volume":"20","author":"Mohammed","year":"2022","journal-title":"J Thromb Haemost"},{"key":"10.1016\/j.jtha.2026.02.015_bib33","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.cll.2009.04.002","article-title":"The laboratory approach to inherited and acquired coagulation factor deficiencies","volume":"29","author":"Wagenman","year":"2009","journal-title":"Clin Lab Med"},{"key":"10.1016\/j.jtha.2026.02.015_bib34","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1046\/j.1365-2141.2003.04256.x","article-title":"Haemostasis and Thrombosis Task Force of the British Committee for Standards in Haematology. Guidelines on fibrinogen assays","volume":"121","author":"Mackie","year":"2003","journal-title":"Br J Haematol"},{"issue":"Suppl 1","key":"10.1016\/j.jtha.2026.02.015_bib35","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/ijlh.12345","article-title":"Laboratory diagnosis of von Willebrand disease","volume":"37","author":"Roberts","year":"2015","journal-title":"Int J Lab Hematol"},{"key":"10.1016\/j.jtha.2026.02.015_bib36","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.cca.2021.10.021","article-title":"Evaluation of thrombin generation assay in factor XI deficiency","volume":"523","author":"Kasonga","year":"2021","journal-title":"Clin Chim Acta"},{"key":"10.1016\/j.jtha.2026.02.015_bib37","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1056\/NEJMoa1613303","article-title":"Thromboprophylaxis after knee arthroscopy and lower-leg casting","volume":"376","author":"van Adrichem","year":"2017","journal-title":"N Engl J Med"},{"key":"10.1016\/j.jtha.2026.02.015_bib38","doi-asserted-by":"crossref","DOI":"10.1111\/ene.70063","article-title":"Coagulation factors and white matter hyperintensities in middle-aged women with and without migraine and ischemic stroke","volume":"32","author":"Ali","year":"2025","journal-title":"Eur J Neurol"},{"key":"10.1016\/j.jtha.2026.02.015_bib39","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1373\/clinchem.2015.250563","article-title":"Recommendations for the generation, quantification, storage, and handling of peptides used for mass spectrometry-based assays","volume":"62","author":"Hoofnagle","year":"2016","journal-title":"Clin Chem"},{"key":"10.1016\/j.jtha.2026.02.015_bib40","unstructured":"Clinical and Laboratory Standards Institute. EP15-A3: user verification of precision and estimation of bias; approved guideline\u2014third edition. 2014. https:\/\/clsi.org\/shop\/standards\/ep15\/. [accessed February 11, 2026]."},{"key":"10.1016\/j.jtha.2026.02.015_bib41","unstructured":"Clinical and Laboratory Standards Institute. Measurement procedure comparison and bias estimation using patient samples. CLSI guideline EP09c 2018. https:\/\/clsi.org\/shop\/standards\/ep09\/. [accessed February 11, 2026]."},{"key":"10.1016\/j.jtha.2026.02.015_bib42","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.jmsacl.2023.01.002","article-title":"Effects of sample matrix in the measurement of antithrombin by LC-MS: a role for immunocapture","volume":"27","author":"Kruijt","year":"2023","journal-title":"J Mass Spectrom Adv Clin Lab"},{"key":"10.1016\/j.jtha.2026.02.015_bib43","unstructured":"Clinical and Laboratory Standards Institute. CLSI EP10. Preliminary evaluation of quantitative medical laboratory measurement procedures. 2024. https:\/\/clsi.org\/shop\/standards\/ep10\/. [accessed February 11, 2026]."},{"key":"10.1016\/j.jtha.2026.02.015_bib44","unstructured":"Clinical and Laboratory Standards Institute. CLSI EP06: evaluation of linearity of quantitative measurement procedures. 2020. https:\/\/clsi.org\/shop\/standards\/ep06\/. [accessed February 11, 2026]."},{"key":"10.1016\/j.jtha.2026.02.015_bib45","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1515\/cclm-2022-0735","article-title":"Quantitative protein mass-spectrometry requires a standardized pre-analytical phase","volume":"61","author":"Smit","year":"2023","journal-title":"Clin Chem Lab Med"},{"key":"10.1016\/j.jtha.2026.02.015_bib46","doi-asserted-by":"crossref","first-page":"5304","DOI":"10.1021\/acs.jproteome.1c00532","article-title":"Development and provisional validation of a multiplex LC-MRM-MS test for timely kidney injury detection in urine","volume":"20","author":"van Duijl","year":"2021","journal-title":"J Proteome Res"},{"key":"10.1016\/j.jtha.2026.02.015_bib47","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1182\/bloodadvances.2024014601","article-title":"Amelioration of a von Willebrand disease type 2B phenotype in vivo upon treatment with allele-selective siRNAs","volume":"9","author":"Linthorst","year":"2025","journal-title":"Blood Adv"},{"issue":"Suppl 6","key":"10.1016\/j.jtha.2026.02.015_bib48","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/hae.14046","article-title":"WFH Guidelines for the Management of Hemophilia panelists and co-authors. WFH guidelines for the management of hemophilia, 3rd edition","volume":"26","author":"Srivastava","year":"2020","journal-title":"Haemophilia"},{"key":"10.1016\/j.jtha.2026.02.015_bib49","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.cca.2013.09.018","article-title":"From biomarkers to medical tests: the changing landscape of test evaluation","volume":"427","author":"Horvath","year":"2014","journal-title":"Clin Chim Acta"}],"container-title":["Journal of Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1538783626001327?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1538783626001327?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T12:05:32Z","timestamp":1780574732000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1538783626001327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["S1538783626001327"],"URL":"https:\/\/doi.org\/10.1016\/j.jtha.2026.02.015","relation":{},"ISSN":["1538-7836"],"issn-type":[{"value":"1538-7836","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Advancing precision diagnostics in coagulation: introduction and analytical validation of a multiplex mass spectrometry\u2013based test for 5 coagulation factors","name":"articletitle","label":"Article Title"},{"value":"Journal of Thrombosis and Haemostasis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jtha.2026.02.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc. on behalf of International Society on Thrombosis and Haemostasis.","name":"copyright","label":"Copyright"}]}}