{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:25:20Z","timestamp":1788963920065,"version":"build-2803163510"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100019562","name":"Synoptik-Fonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019562","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001734","name":"K\u00f8benhavns Universitet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001734","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007819","name":"Allergan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007819","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008397","name":"Velux Fonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008397","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Ocular Surface"],"published-print":{"date-parts":[[2021,1]]},"DOI":"10.1016\/j.jtos.2020.09.003","type":"journal-article","created":{"date-parts":[[2020,9,12]],"date-time":"2020-09-12T20:09:42Z","timestamp":1599941382000},"page":"210-217","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":75,"special_numbering":"C","title":["Ocular surface microbiota in patients with aqueous tear-deficient dry eye"],"prefix":"10.1016","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2481-1791","authenticated-orcid":false,"given":"Jasmine","family":"Andersson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef K.","family":"Vogt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlene D.","family":"Dalgaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oluf","family":"Pedersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim","family":"Holmgaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Heegaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jtos.2020.09.003_bib1","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.3390\/jcm8091439","article-title":"Dry eye disease: emerging approaches to disease analysis and therapy","volume":"8","author":"Heidari","year":"2019","journal-title":"J Clin Med"},{"key":"10.1016\/j.jtos.2020.09.003_bib3","first-page":"71","article-title":"Pathophysiology, diagnosis and treatment of dry eye","volume":"112","author":"Messmer","year":"2015","journal-title":"Dtsch Arztebl Int"},{"issue":"3","key":"10.1016\/j.jtos.2020.09.003_bib38","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.jtos.2017.05.008","article-title":"TFOS DEWS II definition and classification report","volume":"15","author":"Craig","year":"2017","journal-title":"Ocul Surf"},{"key":"10.1016\/j.jtos.2020.09.003_bib4","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1007\/s12275-019-9127-2","article-title":"Comparative portrayal of ocular surface microbe with and without dry eye","volume":"57","author":"Li","year":"2019","journal-title":"J Microbiol"},{"key":"10.1016\/j.jtos.2020.09.003_bib5","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.2147\/IDR.S162135","article-title":"Pathogens in the Meibomian gland and conjunctival sac: microbiome of normal subjects and patients with Meibomian gland dysfunction","volume":"11","author":"Jiang","year":"2018","journal-title":"Infect Drug Resist"},{"key":"10.1016\/j.jtos.2020.09.003_bib6","doi-asserted-by":"crossref","first-page":"5616","DOI":"10.1167\/iovs.07-0588","article-title":"Ocular pathogen or commensal: a PCR-based study of surface bacterial flora in normal and dry eyes","volume":"48","author":"Graham","year":"2007","journal-title":"Investig Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2020.09.003_bib7","doi-asserted-by":"crossref","first-page":"4774","DOI":"10.1167\/iovs.19-27719","article-title":"Composition and diversity of bacterial community on the ocular surface of patients with meibomian gland dysfunction","volume":"60","author":"Dong","year":"2019","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2020.09.003_bib8","doi-asserted-by":"crossref","DOI":"10.1038\/srep23561","article-title":"Altered mucosal microbiome diversity and disease severity in sj\u00f6gren syndrome","volume":"6","author":"De Paiva","year":"2016","journal-title":"Sci Rep"},{"key":"10.1016\/j.jtos.2020.09.003_bib9","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3109\/08830185.2012.748052","article-title":"Dry eye as a mucosal autoimmune disease","volume":"32","author":"Stern","year":"2013","journal-title":"Int Rev Immunol"},{"key":"10.1016\/j.jtos.2020.09.003_bib10","doi-asserted-by":"crossref","first-page":"2006","DOI":"10.1167\/iovs.10-6997f","article-title":"The international workshop on meibomian gland dysfunction: report of the diagnosis subcommittee","volume":"52","author":"Tomlinson","year":"2011","journal-title":"Investig Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2020.09.003_bib11","doi-asserted-by":"crossref","first-page":"3419","DOI":"10.1038\/srep03419","article-title":"International chronic ocular graft-vs-host-disease (GVHD) Consensus group: proposed diagnostic criteria for chronic GVHD (Part I)","volume":"3","author":"Ogawa","year":"2013","journal-title":"Sci Rep"},{"key":"10.1016\/j.jtos.2020.09.003_bib12","first-page":"1","article-title":"16S metagenomic sequencing library preparation - preparing 16S ribosomal RNA gene amplicons for the Illumina MiSeq system","year":"2013","journal-title":"16S Metagenomic Seq Libr Prep Man"},{"key":"10.1016\/j.jtos.2020.09.003_bib13","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: high-resolution sample inference from Illumina amplicon data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat Methods"},{"key":"10.1016\/j.jtos.2020.09.003_bib14","doi-asserted-by":"crossref","first-page":"352","DOI":"10.32614\/RJ-2016-025","article-title":"Using DECIPHER v2.0 to analyze big biological sequence data in R","volume":"8","author":"Wright","year":"2016","journal-title":"R J"},{"key":"10.1016\/j.jtos.2020.09.003_bib15","doi-asserted-by":"crossref","DOI":"10.1186\/s40168-018-0605-2","article-title":"Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data","author":"Davis","year":"2018","journal-title":"Microbiome"},{"key":"10.1016\/j.jtos.2020.09.003_bib16","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0061217","article-title":"Phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data","volume":"8","author":"McMurdie","year":"2013","journal-title":"PloS One"},{"key":"10.1016\/j.jtos.2020.09.003_bib17","first-page":"2","article-title":"vegan: community Ecology Package. R package version 2.5-2","volume":"1","author":"Oksanen","year":"2019","journal-title":"Crane Rev"},{"key":"10.1016\/j.jtos.2020.09.003_bib18","series-title":"Ggpubr: \u201cggplot2\u201d based publication ready plots","author":"Kassambara","year":"2019"},{"key":"10.1016\/j.jtos.2020.09.003_bib19","article-title":"pairwiseAdonis: pairwise multilevel comparison using Adonis","author":"Martinez Arbizu","year":"2017","journal-title":"R package version"},{"key":"10.1016\/j.jtos.2020.09.003_bib20","unstructured":"Guo K MB. VennDetail: a package for visualization and extract details. R package version 1.2.0 2019."},{"key":"10.1016\/j.jtos.2020.09.003_bib21","doi-asserted-by":"crossref","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol"},{"key":"10.1016\/j.jtos.2020.09.003_bib22","article-title":"Publication-ready volcano plots with enhanced colouring and labeling","volume":"1\u20138","author":"Blighe","year":"2019","journal-title":"R-Package"},{"key":"10.1016\/j.jtos.2020.09.003_bib23","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2011-12-6-r60","article-title":"Metagenomic biomarker discovery and explanation","volume":"12","author":"Segata","year":"2011","journal-title":"Genome Biol"},{"key":"10.1016\/j.jtos.2020.09.003_bib24","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00198-16","article-title":"Changes in the eye microbiota associated with contact lens wearing","volume":"7","author":"Shin","year":"2016","journal-title":"mBio"},{"key":"10.1016\/j.jtos.2020.09.003_bib26","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-10494-9","article-title":"Temporal stability and composition of the ocular surface microbiome","volume":"7","author":"Ozkan","year":"2017","journal-title":"Sci Rep"},{"key":"10.1016\/j.jtos.2020.09.003_bib27","article-title":"Biogeography of the human ocular microbiota","author":"Ozkan","year":"2018","journal-title":"Ocul Surf"},{"key":"10.1016\/j.jtos.2020.09.003_bib28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13073-014-0099-x","article-title":"The conjunctival microbiome in health and trachomatous disease: a case control study","volume":"6","author":"Zhou","year":"2014","journal-title":"Genome Med"},{"key":"10.1016\/j.jtos.2020.09.003_bib29","doi-asserted-by":"crossref","first-page":"5408","DOI":"10.1167\/iovs.10-6939","article-title":"Diversity of bacteria at healthy human conjunctiva","volume":"52","author":"Dong","year":"2011","journal-title":"Investig Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2020.09.003_bib30","doi-asserted-by":"crossref","first-page":"5116","DOI":"10.1167\/iovs.16-19803","article-title":"Paucibacterial microbiome and resident DNA virome of the healthy conjunctiva","volume":"57","author":"Doan","year":"2016","journal-title":"Investig Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2020.09.003_bib31","doi-asserted-by":"crossref","first-page":"6030","DOI":"10.1167\/iovs.17-22957","article-title":"The influence of age and sex on ocular surface microbiota in healthy adults","volume":"58","author":"Wen","year":"2017","journal-title":"Investig Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2020.09.003_bib32","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/ceo.12810","article-title":"Ocular surface microbiome in meibomian gland dysfunction","volume":"45","author":"Watters","year":"2017","journal-title":"Clin Exp Ophthalmol"},{"key":"10.1016\/j.jtos.2020.09.003_bib33","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.jtos.2016.12.003","article-title":"Bacteriological profile of ocular surface flora in meibomian gland dysfunction","volume":"15","author":"Zhang","year":"2017","journal-title":"Ocul Surf"},{"key":"10.1016\/j.jtos.2020.09.003_bib34","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1097\/01.ico.0000225716.85382.7b","article-title":"Effect of antibacterial honey on the ocular flora in tear deficiency and meibomian gland disease","volume":"25","author":"Albietz","year":"2006","journal-title":"Cornea"},{"key":"10.1016\/j.jtos.2020.09.003_bib35","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.jtos.2019.02.001","article-title":"Commensal microflora in human conjunctiva; characteristics of microflora in the patients with chronic ocular graft-versus-host disease","volume":"17","author":"Shimizu","year":"2019","journal-title":"Ocul Surf"},{"key":"10.1016\/j.jtos.2020.09.003_bib36","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1101\/gr.085464.108","article-title":"Microbial community profiling for human microbiome projects: tools, techniques, and challenges","volume":"19","author":"Hamady","year":"2009","journal-title":"Genome Res"},{"key":"10.1016\/j.jtos.2020.09.003_bib37","article-title":"The ocular microbiome: molecular characterisation of a unique and low microbial environment","volume":"1\u201310","author":"Ozkan","year":"2019","journal-title":"Curr Eye Res"}],"container-title":["The Ocular Surface"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1542012420301440?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1542012420301440?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:40:43Z","timestamp":1761540043000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1542012420301440"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":36,"alternative-id":["S1542012420301440"],"URL":"https:\/\/doi.org\/10.1016\/j.jtos.2020.09.003","relation":{},"ISSN":["1542-0124"],"issn-type":[{"value":"1542-0124","type":"print"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Ocular surface microbiota in patients with aqueous tear-deficient dry eye","name":"articletitle","label":"Article Title"},{"value":"The Ocular Surface","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jtos.2020.09.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}