{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T08:41:16Z","timestamp":1780648876152,"version":"3.54.1"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Ocular Surface"],"published-print":{"date-parts":[[2023,10]]},"DOI":"10.1016\/j.jtos.2023.07.010","type":"journal-article","created":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T11:38:26Z","timestamp":1690630706000},"page":"42-50","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Gene expression signatures in conjunctival fornix aspirates of patients with dry eye disease associated with Meibomian gland dysfunction. A proof-of-concept study"],"prefix":"10.1016","volume":"30","author":[{"given":"Carlos","family":"Verg\u00e9s","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2575-1225","authenticated-orcid":false,"given":"Ana","family":"Gim\u00e9nez-Capit\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f3nica","family":"Ribas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Salgado-Borges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesc","family":"March de Ribot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Mayo-de-las-Casas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noelia","family":"Armiger-Borras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Pedraz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel \u00c1ngel","family":"Molina-Vila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jtos.2023.07.010_bib1","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.exger.2012.03.020","article-title":"Aging and dry eye disease","volume":"47","author":"Ding","year":"2012","journal-title":"Exp Gerontol"},{"key":"10.1016\/j.jtos.2023.07.010_bib2","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1136\/bjophthalmol-2015-307415","article-title":"Revisiting the vicious circle of dry eye disease: a focus on the pathophysiology of meibomian gland dysfunction","volume":"100","author":"Baudouin","year":"2016","journal-title":"Br J Ophthalmol"},{"key":"10.1016\/j.jtos.2023.07.010_bib3","doi-asserted-by":"crossref","first-page":"1922","DOI":"10.1167\/iovs.10-6997a","article-title":"The international workshop on meibomian gland dysfunction: executive summary","volume":"52","author":"Nichols","year":"2011","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2023.07.010_bib4","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.jtos.2018.06.005","article-title":"Altered expression level of inflammation-related genes and long-term changes in ocular surface after trabeculectomy, a prospective cohort study","volume":"16","author":"Tong","year":"2018","journal-title":"Ocul Surf"},{"key":"10.1016\/j.jtos.2023.07.010_bib5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1167\/iovs.61.3.1","article-title":"The role of hypoxia-inducible factor 1 alpha in the regulation of human meibomian gland epithelial cells","volume":"61","author":"Liu","year":"2020","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2023.07.010_bib6","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.jtos.2018.12.001","article-title":"Hypoxia: a breath of fresh air for the meibomian gland","volume":"17","author":"Liu","year":"2019","journal-title":"Ocul Surf"},{"key":"10.1016\/j.jtos.2023.07.010_bib7","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1167\/iovs.11-8551","article-title":"Differences in human meibum lipid composition with meibomian gland dysfunction using NMR and principal component analysis","volume":"53","author":"Borchman","year":"2012","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2023.07.010_bib8","doi-asserted-by":"crossref","first-page":"5616","DOI":"10.1167\/iovs.07-0588","article-title":"Ocular pathogen or commensal: a PCR-based study of surface bacterial flora in normal and dry eyes","volume":"48","author":"Graham","year":"2007","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2023.07.010_bib9","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.preteyeres.2012.02.003","article-title":"Ocular surface immunity: homeostatic mechanisms and their disruption in dry eye disease","volume":"31","author":"Barabino","year":"2012","journal-title":"Prog Retin Eye Res"},{"key":"10.1016\/j.jtos.2023.07.010_bib10","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3109\/08830185.2012.748052","article-title":"Dry eye as a mucosal autoimmune disease","volume":"32","author":"Stern","year":"2013","journal-title":"Int Rev Immunol"},{"key":"10.1016\/j.jtos.2023.07.010_bib11","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.jtos.2013.07.003","article-title":"Role of hyperosmolarity in the pathogenesis and management of dry eye disease: proceedings of the OCEAN group meeting","volume":"11","author":"Baudouin","year":"2013","journal-title":"Ocul Surf"},{"key":"10.1016\/j.jtos.2023.07.010_bib12","doi-asserted-by":"crossref","first-page":"2271","DOI":"10.3389\/fimmu.2018.02271","article-title":"Conjunctival inflammatory gene expression profiling in dry eye disease: correlations with HLA-DRA and HLA-DRB1","volume":"9","author":"Kessal","year":"2018","journal-title":"Front Immunol"},{"key":"10.1016\/j.jtos.2023.07.010_bib13","first-page":"1458","article-title":"Flow cytometry in impression cytology specimens. A new method for evaluation of conjunctival inflammation","volume":"38","author":"Baudouin","year":"1997","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2023.07.010_bib14","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1007\/978-1-4615-0717-8_107","article-title":"Flow cytometric analysis of the inflammatory marker HLA DR in dry eye syndrome: results from 12 months of randomized treatment with topical cyclosporin A","volume":"506","author":"Baudouin","year":"2002","journal-title":"Adv Exp Med Biol"},{"key":"10.1016\/j.jtos.2023.07.010_bib15","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1076\/ceyr.19.3.201.5309","article-title":"Altered cytokine balance in the tear fluid and conjunctiva of patients with Sjogren's syndrome keratoconjunctivitis sicca","volume":"19","author":"Pflugfelder","year":"1999","journal-title":"Curr Eye Res"},{"key":"10.1016\/j.jtos.2023.07.010_bib16","first-page":"2283","article-title":"Pro- and anti-inflammatory forms of interleukin-1 in the tear fluid and conjunctiva of patients with dry-eye disease","volume":"42","author":"Solomon","year":"2001","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2023.07.010_bib17","first-page":"862","article-title":"Tear cytokine and chemokine analysis and clinical correlations in evaporative-type dry eye disease","volume":"16","author":"Enriquez-de-Salamanca","year":"2010","journal-title":"Mol Vis"},{"key":"10.1016\/j.jtos.2023.07.010_bib18","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1373\/clinchem.2016.265314","article-title":"Identification of ALK, ROS1, and RET fusions by a multiplexed mRNA-based assay in formalin-fixed, Paraffin-embedded samples from advanced non-small-cell lung cancer patients","volume":"63","author":"Reguart","year":"2017","journal-title":"Clin Chem"},{"key":"10.1016\/j.jtos.2023.07.010_bib19","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1002\/1878-0261.12861","article-title":"Multiplex RNA-based detection of clinically relevant MET alterations in advanced non-small cell lung cancer","volume":"15","author":"Aguado","year":"2021","journal-title":"Mol Oncol"},{"key":"10.1016\/j.jtos.2023.07.010_bib20","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1093\/clinchem\/hvaa248","article-title":"Multiplex detection of clinically relevant mutations in liquid biopsies of cancer patients using a hybridization-based platform","volume":"67","author":"Gimenez-Capitan","year":"2021","journal-title":"Clin Chem"},{"key":"10.1016\/j.jtos.2023.07.010_bib21","doi-asserted-by":"crossref","first-page":"2006","DOI":"10.1167\/iovs.10-6997f","article-title":"The international workshop on meibomian gland dysfunction: report of the diagnosis subcommittee","volume":"52","author":"Tomlinson","year":"2011","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2023.07.010_bib22","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1016\/j.jtos.2017.08.003","article-title":"TFOS DEWS II report executive summary","volume":"15","author":"Craig","year":"2017","journal-title":"Ocul Surf"},{"key":"10.1016\/j.jtos.2023.07.010_bib24","doi-asserted-by":"crossref","DOI":"10.3390\/pharmaceutics14102034","article-title":"Multiplex analysis of CircRNAs from plasma extracellular vesicle-enriched samples for the detection of early-stage non-small cell lung cancer","volume":"14","author":"Pedraz-Valdunciel","year":"2022","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.jtos.2023.07.010_bib25","doi-asserted-by":"crossref","first-page":"3712","DOI":"10.1038\/s41598-021-83132-0","article-title":"Analysis of extracellular vesicle mRNA derived from plasma using the nCounter platform","volume":"11","author":"Bracht","year":"2021","journal-title":"Sci Rep"},{"key":"10.1016\/j.jtos.2023.07.010_bib26","doi-asserted-by":"crossref","first-page":"D605","DOI":"10.1093\/nar\/gkaa1074","article-title":"The STRING database in 2021: customizable protein-protein networks, and functional characterization of user-uploaded gene\/measurement sets","volume":"49","author":"Szklarczyk","year":"2021","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.jtos.2023.07.010_bib27","doi-asserted-by":"crossref","first-page":"695","DOI":"10.3389\/fgene.2019.00695","article-title":"Identification of potential crucial genes and key pathways in breast cancer using bioinformatic analysis","volume":"10","author":"Deng","year":"2019","journal-title":"Front Genet"},{"key":"10.1016\/j.jtos.2023.07.010_bib28","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0235413","article-title":"RNA from stabilized whole blood enables more comprehensive immune gene expression profiling compared to RNA from peripheral blood mononuclear cells","volume":"15","author":"van der Sijde","year":"2020","journal-title":"PLoS One"},{"key":"10.1016\/j.jtos.2023.07.010_bib29","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1001\/archophthalmol.2011.364","article-title":"Dry eye disease: an immune-mediated ocular surface disorder","volume":"130","author":"Stevenson","year":"2012","journal-title":"Arch Ophthalmol"},{"key":"10.1016\/j.jtos.2023.07.010_bib30","article-title":"Analysis of Th17-associated cytokines and clinical correlations in patients with dry eye disease","volume":"12","author":"Liu","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.jtos.2023.07.010_bib31","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.1167\/iovs.10-6482","article-title":"Changes in gene expression in human meibomian gland dysfunction","volume":"52","author":"Liu","year":"2011","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2023.07.010_bib32","doi-asserted-by":"crossref","first-page":"4570","DOI":"10.1167\/iovs.15-16736","article-title":"Gene expression-based predictive models of graft versus host disease-associated dry eye","volume":"56","author":"Cocho","year":"2015","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2023.07.010_bib33","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.702755","article-title":"Differentially expressed gene pathways in the conjunctiva of sjogren syndrome keratoconjunctivitis sicca","volume":"12","author":"de Paiva","year":"2021","journal-title":"Front Immunol"},{"key":"10.1016\/j.jtos.2023.07.010_bib34","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.jtos.2019.03.005","article-title":"Correlation of clinical symptoms and signs with conjunctival gene expression in primary Sjogren syndrome dry eye patients","volume":"17","author":"Liang","year":"2019","journal-title":"Ocul Surf"},{"key":"10.1016\/j.jtos.2023.07.010_bib35","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.preteyeres.2012.06.002","article-title":"Tear analysis in ocular surface diseases","volume":"31","author":"Zhou","year":"2012","journal-title":"Prog Retin Eye Res"},{"key":"10.1016\/j.jtos.2023.07.010_bib36","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1007\/978-1-4615-0717-8_76","article-title":"Changes in the diurnal pattern of the distribution of gelatinases and associated proteins in normal and pathological tear fluids: evidence that the PMN cell is a major source of MMP activity in tear fluid","volume":"506","author":"Sack","year":"2002","journal-title":"Adv Exp Med Biol"},{"key":"10.1016\/j.jtos.2023.07.010_bib37","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.exer.2013.12.017","article-title":"Intra- and inter-day variation of cytokines and chemokines in tears of healthy subjects","volume":"120","author":"Benito","year":"2014","journal-title":"Exp Eye Res"},{"key":"10.1016\/j.jtos.2023.07.010_bib38","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1089\/jop.2021.0121","article-title":"Autophagy protects ocular surface against overactivated inflammation by degrading retinoic acid-induced gene-I in human conjunctival epithelial cells","volume":"38","author":"Zhou","year":"2022","journal-title":"J Ocul Pharmacol Therapeut"},{"key":"10.1016\/j.jtos.2023.07.010_bib39","doi-asserted-by":"crossref","first-page":"e631","DOI":"10.6061\/clinics\/2019\/e631","article-title":"The evaluation of the Myxovirus Resistance 1 protein in serum and saliva to monitor disease activation in primary Sjogren's syndrome","volume":"74","author":"Aydemir","year":"2019","journal-title":"Clinics"},{"key":"10.1016\/j.jtos.2023.07.010_bib40","first-page":"1720","article-title":"MUC1 expression in Sjogren's syndrome, KCS, and control subjects","volume":"16","author":"Caffery","year":"2010","journal-title":"Mol Vis"},{"key":"10.1016\/j.jtos.2023.07.010_bib41","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1186\/s12864-015-1511-7","article-title":"NF-kappaB and IRF pathways: cross-regulation on target genes promoter level","volume":"16","author":"Iwanaszko","year":"2015","journal-title":"BMC Genom"},{"key":"10.1016\/j.jtos.2023.07.010_bib42","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/j.exer.2009.07.001","article-title":"Localization of complement 1 inhibitor (C1INH\/SERPING1) in human eyes with age-related macular degeneration","volume":"89","author":"Mullins","year":"2009","journal-title":"Exp Eye Res"},{"key":"10.1016\/j.jtos.2023.07.010_bib43","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.devcel.2005.10.010","article-title":"IFITM\/Mil\/fragilis family proteins IFITM1 and IFITM3 play distinct roles in mouse primordial germ cell homing and repulsion","volume":"9","author":"Tanaka","year":"2005","journal-title":"Dev Cell"},{"key":"10.1016\/j.jtos.2023.07.010_bib44","doi-asserted-by":"crossref","first-page":"S161","DOI":"10.1016\/S1542-0124(12)70246-X","article-title":"Evaluation of ocular surface inflammation in the presence of dry eye and allergic conjunctival disease","volume":"3","author":"Stern","year":"2005","journal-title":"Ocul Surf"},{"key":"10.1016\/j.jtos.2023.07.010_bib45","doi-asserted-by":"crossref","first-page":"2553","DOI":"10.1167\/iovs.07-0069","article-title":"Dry eye-induced conjunctival epithelial squamous metaplasia is modulated by interferon-gamma","volume":"48","author":"De Paiva","year":"2007","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2023.07.010_bib46","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1038\/s41598-020-58337-4","article-title":"Cathepsin S activation contributes to elevated CX3CL1 (fractalkine) levels in tears of a Sjogren's syndrome murine model","volume":"10","author":"Fu","year":"2020","journal-title":"Sci Rep"},{"key":"10.1016\/j.jtos.2023.07.010_bib47","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S1074-7613(00)80020-5","article-title":"Cathepsin S required for normal MHC class II peptide loading and germinal center development","volume":"10","author":"Shi","year":"1999","journal-title":"Immunity"},{"key":"10.1016\/j.jtos.2023.07.010_bib48","doi-asserted-by":"crossref","first-page":"3418","DOI":"10.1167\/iovs.09-3684","article-title":"Regulation of T-cell chemotaxis by programmed death-ligand 1 (PD-L1) in dry eye-associated corneal inflammation","volume":"51","author":"El Annan","year":"2010","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2023.07.010_bib49","doi-asserted-by":"crossref","first-page":"2561","DOI":"10.1167\/iovs.07-0002","article-title":"Expression of Th-1 chemokines and chemokine receptors on the ocular surface of C57BL\/6 mice: effects of desiccating stress","volume":"48","author":"Yoon","year":"2007","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"10.1016\/j.jtos.2023.07.010_bib50","article-title":"Advances in dry eye disease examination techniques","volume":"8","author":"Wu","year":"2021","journal-title":"Front Med"},{"key":"10.1016\/j.jtos.2023.07.010_bib51","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.jtos.2015.10.004","article-title":"The matrix metalloproteinase 9 point-of-care test in dry eye","volume":"14","author":"Lanza","year":"2016","journal-title":"Ocul Surf"}],"container-title":["The Ocular Surface"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1542012423000964?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1542012423000964?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:50:20Z","timestamp":1761540620000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1542012423000964"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10]]},"references-count":50,"alternative-id":["S1542012423000964"],"URL":"https:\/\/doi.org\/10.1016\/j.jtos.2023.07.010","relation":{},"ISSN":["1542-0124"],"issn-type":[{"value":"1542-0124","type":"print"}],"subject":[],"published":{"date-parts":[[2023,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Gene expression signatures in conjunctival fornix aspirates of patients with dry eye disease associated with Meibomian gland dysfunction. A proof-of-concept study","name":"articletitle","label":"Article Title"},{"value":"The Ocular Surface","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jtos.2023.07.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}