{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T10:55:05Z","timestamp":1785840905219,"version":"3.56.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000615","name":"Fight for Sight","doi-asserted-by":"publisher","award":["5181\/5182"],"award-info":[{"award-number":["5181\/5182"]}],"id":[{"id":"10.13039\/501100000615","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000774","name":"Newcastle University","doi-asserted-by":"publisher","award":["HEIF\/H064"],"award-info":[{"award-number":["HEIF\/H064"]}],"id":[{"id":"10.13039\/501100000774","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100002089","name":"Fight for Sight","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002089","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"UK Research and Innovation Medical Research Council","doi-asserted-by":"publisher","award":["G0900879"],"award-info":[{"award-number":["G0900879"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Ocular Surface"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.jtos.2025.08.005","type":"journal-article","created":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T13:15:37Z","timestamp":1755695737000},"page":"266-273","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["IVCM image analysis for limbal stem cell deficiency: quantitative diagnostics of the corneal epithelium post-transplant recovery"],"prefix":"10.1016","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1549-0046","authenticated-orcid":false,"given":"Patrick","family":"Parkinson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Makarenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver J.","family":"Baylis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo S.","family":"Figueiredo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majlinda","family":"Lako","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anvar","family":"Shukurov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco C.","family":"Figueiredo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura E.","family":"Wadkin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.jtos.2025.08.005_bib1","doi-asserted-by":"crossref","first-page":"190","DOI":"10.4103\/ijo.IJO_646_17","article-title":"Anatomy of cornea and ocular surface","volume":"66","author":"Sridhar","year":"2018","journal-title":"Indian J Ophthalmol"},{"issue":"Pt 2","key":"10.1016\/j.jtos.2025.08.005_bib2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1038\/eye.1989.16","article-title":"The anatomy of the limbus","volume":"3","author":"Van Buskirk","year":"1989","journal-title":"Eye (Lond)"},{"issue":"9","key":"10.1016\/j.jtos.2025.08.005_bib3","doi-asserted-by":"crossref","DOI":"10.3390\/cells10092302","article-title":"Corneal epithelial stem cells-physiology, pathophysiology and therapeutic options","volume":"10","author":"Ruan","year":"2021","journal-title":"Cells"},{"issue":"1","key":"10.1016\/j.jtos.2025.08.005_bib4","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.jtos.2017.11.002","article-title":"The diagnosis of limbal stem cell deficiency","volume":"16","author":"Le","year":"2018","journal-title":"Ocul Surf"},{"key":"10.1016\/j.jtos.2025.08.005_bib5","article-title":"Severe chemical eye injuries - clinical outcomes and associated socio-economic factors","volume":"9","author":"Salvador-Culla","year":"2023","journal-title":"Scars Burn Heal"},{"issue":"3","key":"10.1016\/j.jtos.2025.08.005_bib6","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1002\/stem.276","article-title":"Successful clinical implementation of corneal epithelial stem cell therapy for treatment of unilateral limbal stem cell deficiency","volume":"28","author":"Kolli","year":"2010","journal-title":"Stem Cell"},{"issue":"3","key":"10.1016\/j.jtos.2025.08.005_bib7","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1097\/ICO.0000000000001820","article-title":"Global consensus on definition, classification, diagnosis, and staging of limbal stem cell deficiency","volume":"38","author":"Deng","year":"2019","journal-title":"Cornea"},{"issue":"4","key":"10.1016\/j.jtos.2025.08.005_bib8","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1097\/ICU.0000000000001061","article-title":"Limbal stem cell therapy","volume":"35","author":"Bonnet","year":"2024","journal-title":"Curr Opin Ophthalmol"},{"issue":"11","key":"10.1016\/j.jtos.2025.08.005_bib9","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1093\/stcltm\/szad058","article-title":"Ocular surface regeneration by limbal stem cells therapies: state of the art, challenges, and perspectives","volume":"12","author":"Bonnet","year":"2023","journal-title":"Stem Cells Transl Med"},{"key":"10.1016\/j.jtos.2025.08.005_bib10","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.jtos.2025.07.002","article-title":"Prospective consecutive case series of patients with neurotrophic keratopathy associated with unilateral total limbal stem cell deficiency caused by severe ocular surface burns","volume":"38","author":"Figueiredo","year":"2025","journal-title":"Ocul Surf"},{"issue":"1","key":"10.1016\/j.jtos.2025.08.005_bib11","article-title":"Twenty years of limbal epithelial therapy: an update on managing limbal stem cell deficiency","volume":"3","author":"Roberto","year":"2018","journal-title":"BMJ Open Ophthalmol"},{"key":"10.1016\/j.jtos.2025.08.005_bib12","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.ajo.2022.03.015","article-title":"Cultivated autologous limbal epithelial cell transplantation: new frontier in the treatment of limbal stem cell deficiency","volume":"239","author":"Jurkunas","year":"2022","journal-title":"Am J Ophthalmol"},{"issue":"9057","key":"10.1016\/j.jtos.2025.08.005_bib13","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1016\/S0140-6736(96)11188-0","article-title":"Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium","volume":"349","author":"Pellegrini","year":"1997","journal-title":"Lancet"},{"issue":"7","key":"10.1016\/j.jtos.2025.08.005_bib14","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1136\/bjophthalmol-2011-301164","article-title":"Simple limbal epithelial transplantation (SLET): a novel surgical technique for the treatment of unilateral limbal stem cell deficiency","volume":"96","author":"Sangwan","year":"2012","journal-title":"Br J Ophthalmol"},{"issue":"5","key":"10.1016\/j.jtos.2025.08.005_bib15","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/S0161-6420(89)32833-8","article-title":"Limbal autograft transplantation for ocular surface disorders","volume":"96","author":"Keivyon","year":"1989","journal-title":"Ophthalmology"},{"issue":"5","key":"10.1016\/j.jtos.2025.08.005_bib16","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1097\/00003226-199409000-00003","article-title":"Human allograft limbal transplantation for corneal surface reconstruction","volume":"13","author":"Tsai","year":"1994","journal-title":"Cornea"},{"issue":"9","key":"10.1016\/j.jtos.2025.08.005_bib17","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1016\/S0161-6420(01)00694-7","article-title":"Cultivated corneal epithelial stem cell transplantation in ocular surface disorders","volume":"108","author":"Koizumi","year":"2001","journal-title":"Ophthalmology"},{"key":"10.1016\/j.jtos.2025.08.005_bib18","article-title":"Successful management of immunological rejection following allogeneic simple limbal epithelial transplantation (SLET) for bilateral ocular burns","volume":"2013","author":"Bhalekar","year":"2013","journal-title":"Case Rep"},{"issue":"1","key":"10.1016\/j.jtos.2025.08.005_bib19","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1186\/s13287-020-01783-8","article-title":"Ex vivo cultivated oral mucosal epithelial cell transplantation for limbal stem cell deficiency: a review","volume":"11","author":"Cabral","year":"2020","journal-title":"Stem Cell Res Ther"},{"issue":"12","key":"10.1016\/j.jtos.2025.08.005_bib20","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1056\/NEJMoa040455","article-title":"Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium","volume":"351","author":"Nishida","year":"2004","journal-title":"N Engl J Med"},{"issue":"10","key":"10.1016\/j.jtos.2025.08.005_bib21","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1136\/bjo.2003.038497","article-title":"Transplantation of cultivated autologous oral mucosal epithelial cells in patients with severe ocular surface disorders","volume":"88","author":"Nakamura","year":"2004","journal-title":"Br J Ophthalmol"},{"key":"10.1016\/j.jtos.2025.08.005_bib22","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jtos.2020.12.008","article-title":"A systematic review of cellular therapies for the treatment of limbal stem cell deficiency affecting one or both eyes","volume":"20","author":"Figueiredo","year":"2021","journal-title":"Ocul Surf"},{"issue":"8","key":"10.1016\/j.jtos.2025.08.005_bib23","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1002\/stem.1694","article-title":"Successful application of ex vivo expanded human autologous oral mucosal epithelium for the treatment of total bilateral limbal stem cell deficiency","volume":"32","author":"Kolli","year":"2014","journal-title":"Stem Cell"},{"issue":"4","key":"10.1016\/j.jtos.2025.08.005_bib24","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s40778-023-00229-0","article-title":"Human stem cells for ophthalmology: recent advances in diagnostic image analysis and computational modelling","volume":"9","author":"Wadkin","year":"2023","journal-title":"Curr Stem Cell Rep"},{"issue":"19","key":"10.1016\/j.jtos.2025.08.005_bib25","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2117553119","article-title":"Single-cell\u2013resolution map of human retinal pigment epithelium helps discover subpopulations with differential disease sensitivity","volume":"119","author":"Ortolan","year":"2022","journal-title":"Proc Natl Acad Sci"},{"issue":"5","key":"10.1016\/j.jtos.2025.08.005_bib26","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.21037\/qims-20-1052","article-title":"Morphological characterization of the human corneal epithelium by in vivo confocal laser scanning microscopy","volume":"11","author":"Sterenczak","year":"2021","journal-title":"Quant Imag Med Surg"},{"issue":"47","key":"10.1016\/j.jtos.2025.08.005_bib27","doi-asserted-by":"crossref","DOI":"10.3791\/2194","article-title":"Corneal confocal microscopy: a novel non-invasive technique to quantify small fibre pathology in peripheral neuropathies","author":"Tavakoli","year":"2011","journal-title":"J Vis Exp"},{"issue":"3","key":"10.1016\/j.jtos.2025.08.005_bib28","doi-asserted-by":"crossref","first-page":"710","DOI":"10.18063\/ijb.710","article-title":"3D bioprinting as a prospective therapeutic strategy for corneal limbal epithelial stem cell deficiency","volume":"9","author":"Xue","year":"2023","journal-title":"Int J Bioprint"},{"issue":"7","key":"10.1016\/j.jtos.2025.08.005_bib29","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat Methods"},{"key":"10.1016\/j.jtos.2025.08.005_bib30","series-title":"An Introduction to the Bootstrap","author":"Efron","year":"1994"},{"issue":"3","key":"10.1016\/j.jtos.2025.08.005_bib31","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/BF00977785","article-title":"Two algorithms for constructing a Delaunay triangulation","volume":"9","author":"Lee","year":"1980","journal-title":"Int J Comput Inf Sci"},{"issue":"5","key":"10.1016\/j.jtos.2025.08.005_bib32","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1097\/ICO.0b013e31826247bf","article-title":"Characteristic quantities of corneal epithelial structures in confocal laser scanning microscopic volume data sets","volume":"32","author":"Prakasam","year":"2013","journal-title":"Cornea"},{"key":"10.1016\/j.jtos.2025.08.005_bib33","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1186\/s12886-016-0290-5","article-title":"Comparison of human corneal cell density by age and corneal location: an in vivo confocal microscopy study","volume":"16","author":"Zheng","year":"2016","journal-title":"BMC Ophthalmol"},{"issue":"4","key":"10.1016\/j.jtos.2025.08.005_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.ajo.2015.06.026","article-title":"Limbal basal cell density decreases in limbal stem cell deficiency","volume":"160","author":"Chan","year":"2015","journal-title":"Am J Ophthalmol"},{"issue":"5","key":"10.1016\/j.jtos.2025.08.005_bib35","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1097\/00003226-199809000-00005","article-title":"Normal human corneal cell populations evaluated by in vivo scanning slit confocal microscopy","volume":"17","author":"Mustonen","year":"1998","journal-title":"Cornea"},{"key":"10.1016\/j.jtos.2025.08.005_bib36","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.jtos.2020.04.007","article-title":"The utility of anterior segment optical coherence tomography angiography for the assessment of limbal stem cell deficiency","volume":"19","author":"Binotti","year":"2021","journal-title":"Ocul Surf"},{"issue":"6","key":"10.1016\/j.jtos.2025.08.005_bib37","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1097\/00003226-200308000-00009","article-title":"Analysis of limbal stem cell deficiency by corneal impression cytology","volume":"22","author":"Donisi","year":"2003","journal-title":"Cornea"},{"issue":"2","key":"10.1016\/j.jtos.2025.08.005_bib38","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1097\/01.ico.0000170694.90455.f7","article-title":"In vivo investigations of the corneal epithelium with the confocal Rostock Laser Scanning Microscope (RLSM)","volume":"25","author":"Eckard","year":"2006","journal-title":"Cornea"},{"issue":"2","key":"10.1016\/j.jtos.2025.08.005_bib39","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/s00417-014-2812-2","article-title":"Aging and corneal layers: an in vivo corneal confocal microscopy study","volume":"253","author":"Gambato","year":"2015","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"key":"10.1016\/j.jtos.2025.08.005_bib40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/08164622.2025.2550732","article-title":"In vivo confocal microscopy in ophthalmology: research trends from a bibliometric analysis","author":"Kesimal","year":"2025","journal-title":"Clin Exp Optom"}],"container-title":["The Ocular Surface"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1542012425001053?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1542012425001053?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T13:36:38Z","timestamp":1772890598000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1542012425001053"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":40,"alternative-id":["S1542012425001053"],"URL":"https:\/\/doi.org\/10.1016\/j.jtos.2025.08.005","relation":{},"ISSN":["1542-0124"],"issn-type":[{"value":"1542-0124","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"IVCM image analysis for limbal stem cell deficiency: quantitative diagnostics of the corneal epithelium post-transplant recovery","name":"articletitle","label":"Article Title"},{"value":"The Ocular Surface","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jtos.2025.08.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}