{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T22:27:14Z","timestamp":1788992834295,"version":"build-2803163510"},"reference-count":111,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T00:00:00Z","timestamp":1684713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013302","name":"King Abdullah International Medical Research Center","doi-asserted-by":"publisher","award":["RC20\/219"],"award-info":[{"award-number":["RC20\/219"]}],"id":[{"id":"10.13039\/501100013302","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virus Eradication"],"published-print":{"date-parts":[[2023,6]]},"DOI":"10.1016\/j.jve.2023.100327","type":"journal-article","created":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T12:07:21Z","timestamp":1685102841000},"page":"100327","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"title":["Coronaviruses SARS-CoV, MERS-CoV, and SARS-CoV-2 helicase inhibitors: a systematic review of in vitro studies"],"prefix":"10.1016","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4977-2111","authenticated-orcid":false,"given":"Nimer","family":"Mehyar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jve.2023.100327_bib1","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1016\/j.bcp.2012.08.012","article-title":"Development of chemical inhibitors of the SARS coronavirus: viral helicase as a potential target","volume":"84","author":"Keum","year":"2012","journal-title":"Biochem Pharmacol"},{"key":"10.1016\/j.jve.2023.100327_bib2","doi-asserted-by":"crossref","first-page":"4251","DOI":"10.1128\/AAC.03406-14","article-title":"Alternative screening approaches for discovery of Middle East respiratory syndrome coronavirus inhibitors","volume":"58","author":"LaFemina","year":"2014","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.jve.2023.100327_bib3","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.arcmed.2020.05.024","article-title":"Should we try SARS-CoV-2 helicase inhibitors for COVID-19 therapy?","volume":"51","author":"Habtemariam","year":"2020","journal-title":"Arch Med Res"},{"key":"10.1016\/j.jve.2023.100327_bib4","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1080\/13543776.2021.1884224","article-title":"Coronavirus helicases: attractive and unique targets of antiviral drug-development and therapeutic patents","volume":"31","author":"Spratt","year":"2021","journal-title":"Expert Opin Ther Pat"},{"key":"10.1016\/j.jve.2023.100327_bib5","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/s44149-021-00005-9","article-title":"The taxonomy, host range and pathogenicity of coronaviruses and other viruses in the Nidovirales order","volume":"1","author":"Zhou","year":"2021","journal-title":"Anim Dis"},{"key":"10.1016\/j.jve.2023.100327_bib6","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/s41580-021-00432-z","article-title":"Structures and functions of coronavirus replication-transcription complexes and their relevance for SARS-CoV-2 drug design","volume":"23","author":"Malone","year":"2022","journal-title":"Nat Rev Mol Cell Biol"},{"key":"10.1016\/j.jve.2023.100327_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.lfs.2020.117883","article-title":"Potential therapeutic targets for combating SARS-CoV-2: drug repurposing, clinical trials and recent advancements","volume":"256","author":"Pandey","year":"2020","journal-title":"Life Sci"},{"key":"10.1016\/j.jve.2023.100327_bib8","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/s41579-020-00468-6","article-title":"Coronavirus biology and replication: implications for SARS-CoV-2","volume":"19","author":"V'kovski","year":"2021","journal-title":"Nat Rev Microbiol"},{"issue":"37","key":"10.1016\/j.jve.2023.100327_bib9","doi-asserted-by":"crossref","first-page":"12910","DOI":"10.1074\/jbc.REV120.013930","article-title":"The molecular virology of coronaviruses","volume":"295","author":"Hartenian","year":"2020","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jve.2023.100327_bib10","doi-asserted-by":"crossref","first-page":"3464","DOI":"10.1093\/nar\/gkt1310","article-title":"Structural basis for the regulatory function of a complex zinc-binding domain in a replicative arterivirus helicase resembling a nonsense-mediated mRNA decay helicase","volume":"42","author":"Deng","year":"2014","journal-title":"Nucleic Acids Res"},{"issue":"5","key":"10.1016\/j.jve.2023.100327_bib11","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.3390\/cells9051267","article-title":"A structural view of SARS-CoV-2 RNA replication machinery: RNA synthesis, proofreading and final capping","volume":"9","author":"Romano","year":"2020","journal-title":"Cells"},{"key":"10.1016\/j.jve.2023.100327_bib12","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.virusres.2014.12.001","article-title":"What we know but do not understand about nidovirus helicases","volume":"202","author":"Lehmann","year":"2015","journal-title":"Virus Res"},{"key":"10.1016\/j.jve.2023.100327_bib13","article-title":"Mechanism of duplex unwinding by coronavirus nsp13 helicases","author":"Hu","year":"2020","journal-title":"bioRxiv"},{"key":"10.1016\/j.jve.2023.100327_bib14","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1006474","article-title":"Crystal structure of Middle East respiratory syndrome coronavirus helicase","volume":"13","author":"Hao","year":"2017","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.jve.2023.100327_bib15","doi-asserted-by":"crossref","first-page":"6538","DOI":"10.1093\/nar\/gkz409","article-title":"Delicate structural coordination of the severe acute respiratory syndrome coronavirus Nsp13 upon ATP hydrolysis","volume":"47","author":"Jia","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.jve.2023.100327_bib16","doi-asserted-by":"crossref","first-page":"4848","DOI":"10.1038\/s41467-021-25166-6","article-title":"Structure, mechanism and crystallographic fragment screening of the SARS-CoV-2 NSP13 helicase","volume":"12","author":"Newman","year":"2021","journal-title":"Nat Commun"},{"key":"10.1016\/j.jve.2023.100327_bib17","doi-asserted-by":"crossref","first-page":"18265","DOI":"10.1074\/jbc.R600008200","article-title":"Mechanisms of helicases","volume":"281","author":"Patel","year":"2006","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jve.2023.100327_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.meegid.2020.104327","article-title":"Recent progress and challenges in drug development against COVID-19 coronavirus (SARS-CoV-2) - an update on the status","volume":"83","author":"Abd El-Aziz","year":"2020","journal-title":"Infect Genet Evol"},{"key":"10.1016\/j.jve.2023.100327_bib19","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.2217\/fmb-2020-0120","article-title":"Existing antiviral options against SARS-CoV-2 replication in COVID-19 patients","volume":"15","author":"Ghanbari","year":"2020","journal-title":"Future Microbiol"},{"key":"10.1016\/j.jve.2023.100327_bib20","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.3389\/fmicb.2020.01818","article-title":"Antivirals against coronaviruses: candidate drugs for SARS-CoV-2 treatment?","volume":"11","author":"Santos","year":"2020","journal-title":"Front Microbiol"},{"key":"10.1016\/j.jve.2023.100327_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.drup.2020.100721","article-title":"Current status of antivirals and druggable targets of SARS CoV-2 and other human pathogenic coronaviruses","volume":"53","author":"Artese","year":"2020","journal-title":"Drug Resist Updates"},{"key":"10.1016\/j.jve.2023.100327_bib22","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2021.704205","article-title":"The drug repurposing for COVID-19 clinical trials provide very effective therapeutic combinations: lessons learned from major clinical studies","volume":"12","author":"Chakraborty","year":"2021","journal-title":"Front Pharmacol"},{"key":"10.1016\/j.jve.2023.100327_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioorg.2021.105574","article-title":"Virtual screening and in vitro validation of natural compound inhibitors against SARS-CoV-2 spike protein","volume":"119","author":"Power","year":"2022","journal-title":"Bioorg Chem"},{"key":"10.1016\/j.jve.2023.100327_bib24","doi-asserted-by":"crossref","first-page":"4316","DOI":"10.1080\/07391102.2020.1776639","article-title":"Virtual screening-driven drug discovery of SARS-CoV2 enzyme inhibitors targeting viral attachment, replication, post-translational modification and host immunity evasion infection mechanisms","volume":"39","author":"Quimque","year":"2021","journal-title":"J Biomol Struct Dyn"},{"key":"10.1016\/j.jve.2023.100327_bib25","doi-asserted-by":"crossref","first-page":"6862","DOI":"10.1128\/JVI.73.8.6862-6871.1999","article-title":"The putative helicase of the coronavirus mouse hepatitis virus is processed from the replicase gene polyprotein and localizes in complexes that are active in viral RNA synthesis","volume":"73","author":"Denison","year":"1999","journal-title":"J Virol"},{"key":"10.1016\/j.jve.2023.100327_bib26","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1080\/1062936X.2021.1993995","article-title":"Discovery of Zafirlukast as a novel SARS-CoV-2 helicase inhibitor using in silico modelling and a FRET-based assay","volume":"32","author":"Mehyar","year":"2021","journal-title":"SAR QSAR Environ Res"},{"key":"10.1016\/j.jve.2023.100327_bib27","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1080\/1062936X.2020.1857437","article-title":"Using in silico modelling and FRET-based assays in the discovery of novel FDA-approved drugs as inhibitors of MERS-CoV helicase","volume":"32","author":"Mehyar","year":"2021","journal-title":"SAR QSAR Environ Res"},{"key":"10.1016\/j.jve.2023.100327_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.drup.2020.100733","article-title":"COVID-19 update: the race to therapeutic development","volume":"53","author":"Twomey","year":"2020","journal-title":"Drug Resist Updates"},{"key":"10.1016\/j.jve.2023.100327_bib29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/2046-4053-4-1","article-title":"Preferred reporting items for systematic review and meta-analysis protocols (PRISMA-P) 2015 statement","volume":"4","author":"Moher","year":"2015","journal-title":"Syst Rev"},{"key":"10.1016\/j.jve.2023.100327_bib30","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2307\/2529310","article-title":"The measurement of observer agreement for categorical data","volume":"33","author":"Landis","year":"1977","journal-title":"Biometrics"},{"key":"10.1016\/j.jve.2023.100327_bib31","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1111\/j.1742-4658.2010.07961.x","article-title":"On the mechanisms of bananin activity against severe acute respiratory syndrome coronavirus","volume":"278","author":"Wang","year":"2011","journal-title":"FEBS J"},{"key":"10.1016\/j.jve.2023.100327_bib32","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1007\/s40265-020-01321-z","article-title":"The G-quadruplex\/helicase world as a potential antiviral approach against COVID-19","volume":"80","author":"Panera","year":"2020","journal-title":"Drugs"},{"key":"10.1016\/j.jve.2023.100327_bib33","doi-asserted-by":"crossref","first-page":"9144","DOI":"10.1021\/acs.jpclett.0c02421","article-title":"Discovery of COVID-19 inhibitors targeting the SARS-CoV-2 Nsp13 helicase","volume":"11","author":"White","year":"2020","journal-title":"J Phys Chem Lett"},{"key":"10.1016\/j.jve.2023.100327_bib34","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-89724-0","article-title":"Repurposing potential of posaconazole and grazoprevir as inhibitors of SARS-CoV-2 helicase","volume":"11","author":"Abidi","year":"2021","journal-title":"Sci Rep"},{"key":"10.1016\/j.jve.2023.100327_bib35","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.2174\/0929867054637644","article-title":"Synthesis of novel test compounds for antiviral chemotherapy of severe acute respiratory syndrome (SARS)","volume":"12","author":"Kesel","year":"2005","journal-title":"Curr Med Chem"},{"key":"10.1016\/j.jve.2023.100327_bib36","doi-asserted-by":"crossref","first-page":"13205","DOI":"10.1021\/acs.jmedchem.0c00626","article-title":"Chinese therapeutic strategy for fighting COVID-19 and potential small-molecule inhibitors against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)","volume":"63","author":"Choudhry","year":"2020","journal-title":"J Med Chem"},{"key":"10.1016\/j.jve.2023.100327_bib37","doi-asserted-by":"crossref","first-page":"508","DOI":"10.5114\/aoms.2020.94567","article-title":"State-of-the-art tools unveil potent drug targets amongst clinically approved drugs to inhibit helicase in SARS-CoV-2","volume":"16","author":"Borgio","year":"2020","journal-title":"Arch Med Sci"},{"key":"10.1016\/j.jve.2023.100327_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.compbiomed.2020.103848","article-title":"Identification of potential inhibitors of three key enzymes of SARS-CoV2 using computational approach","volume":"122","author":"Iftikhar","year":"2020","journal-title":"Comput Biol Med"},{"key":"10.1016\/j.jve.2023.100327_bib39","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.1016\/j.ijbiomac.2020.09.138","article-title":"Evaluation of the potency of FDA-approved drugs on wild type and mutant SARS-CoV-2 helicase (Nsp13)","volume":"163","author":"Ugurel","year":"2020","journal-title":"Int J Biol Macromol"},{"key":"10.1016\/j.jve.2023.100327_bib40","doi-asserted-by":"crossref","first-page":"9543","DOI":"10.1080\/07391102.2021.1931451","article-title":"Chemical system biology approach to identify multi-targeting FDA inhibitors for treating COVID-19 and associated health complications","volume":"40","author":"Naik","year":"2022","journal-title":"J Biomol Struct Dyn"},{"key":"10.1016\/j.jve.2023.100327_bib41","doi-asserted-by":"crossref","first-page":"12062","DOI":"10.1080\/07391102.2021.1970024","article-title":"Discovering potential inhibitors against SARS-CoV-2 by targeting Nsp13 Helicase","volume":"40","author":"Nandi","year":"2022","journal-title":"J Biomol Struct Dyn"},{"key":"10.1016\/j.jve.2023.100327_bib42","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1134\/S1070363221060098","article-title":"Synthesis of hetaryl-substituted asymmetric porphyrins and their affinity to SARS-CoV-2 helicase","volume":"91","author":"Syrbu","year":"2021","journal-title":"Russ J Gen Chem"},{"key":"10.1016\/j.jve.2023.100327_bib43","doi-asserted-by":"crossref","first-page":"2264","DOI":"10.1080\/07391102.2020.1837679","article-title":"Secondary metabolites from spice and herbs as potential multitarget inhibitors of SARS-CoV-2 proteins","volume":"40","author":"Gupta","year":"2022","journal-title":"J Biomol Struct Dyn"},{"key":"10.1016\/j.jve.2023.100327_bib44","doi-asserted-by":"crossref","first-page":"4718","DOI":"10.1128\/AAC.00957-12","article-title":"Severe acute respiratory syndrome coronavirus replication inhibitor that interferes with the nucleic acid unwinding of the viral helicase","volume":"56","author":"Adedeji","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.jve.2023.100327_bib45","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.3390\/ijms22041581","article-title":"Comparison of antiviral activity of gemcitabine with 2'-fluoro-2'-deoxycytidine and combination therapy with remdesivir against SARS-CoV-2","volume":"22","author":"Jang","year":"2021","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.jve.2023.100327_bib46","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2012201118","article-title":"Bepridil is potent against SARS-CoV-2 in vitro","volume":"118","author":"Vatansever","year":"2021","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jve.2023.100327_bib47","doi-asserted-by":"crossref","first-page":"6978","DOI":"10.3390\/molecules20046978","article-title":"Discovery of metal ions chelator quercetin derivatives with potent anti-HCV activities","volume":"20","author":"Zhong","year":"2015","journal-title":"Molecules"},{"key":"10.1016\/j.jve.2023.100327_bib48","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.02324-20","article-title":"Antiviral peptides targeting the helicase activity of enterovirus nonstructural protein 2C","volume":"95","author":"Fang","year":"2021","journal-title":"J Virol"},{"key":"10.1016\/j.jve.2023.100327_bib49","doi-asserted-by":"crossref","first-page":"25715","DOI":"10.1021\/acsami.1c04641","article-title":"Identification of a direct-acting antiviral agent targeting RNA helicase via a graphene oxide nanobiosensor","volume":"13","author":"Kim","year":"2021","journal-title":"ACS Appl Mater Interfaces"},{"key":"10.1016\/j.jve.2023.100327_bib50","doi-asserted-by":"crossref","first-page":"161","DOI":"10.2174\/187152106776359039","article-title":"The bananins: new anticorona-RNA-viral agents with unique structural signature","volume":"5","author":"Kesel","year":"2006","journal-title":"Anti-Infect Agents Med Chem"},{"key":"10.1016\/j.jve.2023.100327_bib51","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1248\/bpb.b17-00241","article-title":"Comparative genomic analysis MERS CoV isolated from humans and camels with special reference to virus encoded helicase","volume":"40","author":"Alnazawi","year":"2017","journal-title":"Biol Pharm Bull"},{"key":"10.1016\/j.jve.2023.100327_bib52","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0253089","article-title":"SARS-CoV-2 viral proteins NSP1 and NSP13 inhibit interferon activation through distinct mechanisms","volume":"16","author":"Vazquez","year":"2021","journal-title":"PLoS One"},{"key":"10.1016\/j.jve.2023.100327_bib53","doi-asserted-by":"crossref","first-page":"1998","DOI":"10.1016\/j.csbj.2021.04.014","article-title":"Identification of multipotent drugs for COVID-19 therapeutics with the evaluation of their SARS-CoV2 inhibitory activity","volume":"19","author":"Kumar","year":"2021","journal-title":"Comput Struct Biotechnol J"},{"issue":"Suppl 4","key":"10.1016\/j.jve.2023.100327_bib54","first-page":"36","article-title":"Helicases as antiviral drug targets","volume":"14","author":"Huang","year":"2008","journal-title":"Hong Kong Med J"},{"key":"10.1016\/j.jve.2023.100327_bib55","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1016\/j.chembiol.2004.07.013","article-title":"Identification of novel small-molecule inhibitors of severe acute respiratory syndrome-associated coronavirus by chemical genetics","volume":"11","author":"Kao","year":"2004","journal-title":"Chem Biol"},{"key":"10.1016\/j.jve.2023.100327_bib56","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.chembiol.2005.01.006","article-title":"The adamantane-derived bananins are potent inhibitors of the helicase activities and replication of SARS coronavirus","volume":"12","author":"Tanner","year":"2005","journal-title":"Chem Biol"},{"key":"10.1016\/j.jve.2023.100327_bib57","doi-asserted-by":"crossref","first-page":"4413","DOI":"10.1039\/b709515e","article-title":"Inhibition of SARS coronavirus helicase by bismuth complexes","author":"Yang","year":"2007","journal-title":"Chem Commun"},{"key":"10.1016\/j.jve.2023.100327_bib58","doi-asserted-by":"crossref","first-page":"6464","DOI":"10.1002\/anie.200701021","article-title":"Bismuth complexes inhibit the SARS coronavirus","volume":"46","author":"Yang","year":"2007","journal-title":"Angew Chem Int Ed Engl"},{"key":"10.1016\/j.jve.2023.100327_bib59","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1016\/j.bbrc.2007.12.020","article-title":"Isolation of inhibitory RNA aptamers against severe acute respiratory syndrome (SARS) coronavirus NTPase\/Helicase","volume":"366","author":"Jang","year":"2008","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.jve.2023.100327_bib60","doi-asserted-by":"crossref","first-page":"3037","DOI":"10.1002\/cbic.200800491","article-title":"Differential inhibitory activities and stabilisation of DNA aptamers against the SARS coronavirus helicase","volume":"9","author":"Shum","year":"2008","journal-title":"Chembiochem"},{"key":"10.1016\/j.jve.2023.100327_bib61","doi-asserted-by":"crossref","first-page":"1636","DOI":"10.1016\/j.bmcl.2009.02.010","article-title":"Aryl diketoacids (ADK) selectively inhibit duplex DNA-unwinding activity of SARS coronavirus NTPase\/helicase","volume":"19","author":"Lee","year":"2009","journal-title":"Bioorg Med Chem Lett"},{"key":"10.1016\/j.jve.2023.100327_bib62","doi-asserted-by":"crossref","first-page":"4538","DOI":"10.1016\/j.bmcl.2009.07.009","article-title":"Investigation of the pharmacophore space of Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) NTPase\/helicase by dihydroxychromone derivatives","volume":"19","author":"Lee","year":"2009","journal-title":"Bioorg Med Chem Lett"},{"key":"10.1016\/j.jve.2023.100327_bib63","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.3390\/biomedicines9111725","article-title":"In vitro replication inhibitory activity of xanthorrhizol against severe acute respiratory syndrome coronavirus 2","volume":"9","author":"Kim","year":"2021","journal-title":"Biomedicines"},{"key":"10.1016\/j.jve.2023.100327_bib64","doi-asserted-by":"crossref","first-page":"8017","DOI":"10.1128\/JVI.00998-13","article-title":"Novel inhibitors of severe acute respiratory syndrome coronavirus entry that act by three distinct mechanisms","volume":"87","author":"Adedeji","year":"2013","journal-title":"J Virol"},{"key":"10.1016\/j.jve.2023.100327_bib65","doi-asserted-by":"crossref","first-page":"4894","DOI":"10.1128\/AAC.02994-14","article-title":"Evaluation of SSYA10-001 as a replication inhibitor of severe acute respiratory syndrome, mouse hepatitis, and Middle East respiratory syndrome coronaviruses","volume":"58","author":"Adedeji","year":"2014","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.jve.2023.100327_bib66","doi-asserted-by":"crossref","first-page":"4049","DOI":"10.1016\/j.bmcl.2012.04.081","article-title":"Identification of myricetin and scutellarein as novel chemical inhibitors of the SARS coronavirus helicase, nsP13","volume":"22","author":"Yu","year":"2012","journal-title":"Bioorg Med Chem Lett"},{"key":"10.1016\/j.jve.2023.100327_bib67","doi-asserted-by":"crossref","first-page":"3187","DOI":"10.5012\/bkcs.2013.34.11.3187","article-title":"Inhibition of SARS coronavirus helicase by baicalein","volume":"34","author":"Keum","year":"2013","journal-title":"Bull Kor Chem Soc"},{"key":"10.1016\/j.jve.2023.100327_bib68","doi-asserted-by":"crossref","first-page":"2007","DOI":"10.4014\/jmb.1507.07078","article-title":"Identification of a novel small molecule inhibitor against SARS coronavirus helicase","volume":"25","author":"Cho","year":"2015","journal-title":"J Microbiol Biotechnol"},{"key":"10.1016\/j.jve.2023.100327_bib69","doi-asserted-by":"crossref","first-page":"2066","DOI":"10.1002\/bkcs.11002","article-title":"Selective inhibition of enzymatic activities of severe acute respiratory syndrome coronavirus helicase with a thioxopyrimidine derivative","volume":"37","author":"Lee","year":"2016","journal-title":"Bull Kor Chem Soc"},{"key":"10.1016\/j.jve.2023.100327_bib70","doi-asserted-by":"crossref","first-page":"2070","DOI":"10.4014\/jmb.1707.07073","article-title":"A novel chemical compound for inhibition of SARS coronavirus helicase","volume":"27","author":"Lee","year":"2017","journal-title":"J Microbiol Biotechnol"},{"key":"10.1016\/j.jve.2023.100327_bib71","doi-asserted-by":"crossref","first-page":"145","DOI":"10.2478\/acph-2020-0024","article-title":"Design, synthesis and molecular docking of novel triazole derivatives as potential CoV helicase inhibitors","volume":"70","author":"Zaher","year":"2020","journal-title":"Acta Pharm"},{"key":"10.1016\/j.jve.2023.100327_bib72","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s12250-020-00242-1","article-title":"SARS-Coronavirus-2 Nsp13 possesses NTPase and RNA helicase activities that can Be inhibited by bismuth salts","volume":"35","author":"Shu","year":"2020","journal-title":"Virol Sin"},{"key":"10.1016\/j.jve.2023.100327_bib73","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1038\/s41564-020-00802-x","article-title":"Metallodrug ranitidine bismuth citrate suppresses SARS-CoV-2 replication and relieves virus-associated pneumonia in Syrian hamsters","volume":"5","author":"Yuan","year":"2020","journal-title":"Nat Microbiol"},{"key":"10.1016\/j.jve.2023.100327_bib74","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1038\/s41586-021-03431-4","article-title":"Clofazimine broadly inhibits coronaviruses including SARS-CoV-2","volume":"593","author":"Yuan","year":"2021","journal-title":"Nature"},{"issue":"4","key":"10.1016\/j.jve.2023.100327_bib75","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1021\/acsptsci.1c00022","article-title":"Synergistic inhibition of SARS-CoV-2 replication using disulfiram\/ebselen and remdesivir","author":"Chen","year":"2021","journal-title":"ACS Pharmacol Transl Sci"},{"key":"10.1016\/j.jve.2023.100327_bib76","doi-asserted-by":"crossref","first-page":"5698","DOI":"10.1016\/j.ejmech.2011.09.005","article-title":"2,6-Bis-arylmethyloxy-5-hydroxychromones with antiviral activity against both hepatitis C virus (HCV) and SARS-associated coronavirus (SCV)","volume":"46","author":"Kim","year":"2011","journal-title":"Eur J Med Chem"},{"key":"10.1016\/j.jve.2023.100327_bib77","doi-asserted-by":"crossref","first-page":"2405","DOI":"10.1042\/BCJ20210201","article-title":"Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp13 helicase","volume":"478","author":"Zeng","year":"2021","journal-title":"Biochem J"},{"key":"10.1016\/j.jve.2023.100327_bib78","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1021\/acsptsci.1c00253","article-title":"Natural compounds inhibit SARS-CoV-2 nsp13 unwinding and ATPase enzyme activities","volume":"5","author":"Corona","year":"2022","journal-title":"ACS Pharmacol Transl Sci"},{"issue":"5","key":"10.1016\/j.jve.2023.100327_bib79","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1021\/acsmedchemlett.2c00123","article-title":"Discovery of 2-phenylquinolines with broad-spectrum anti-coronavirus activity","volume":"13","author":"Nizi","year":"2022","journal-title":"ACS Med Chem Lett"},{"key":"10.1016\/j.jve.2023.100327_bib80","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1021\/acsinfecdis.2c00165","article-title":"Kinetic characterization of SARS-CoV-2 nsp13 ATPase activity and discovery of small-molecule inhibitors","volume":"8","author":"Yazdi","year":"2022","journal-title":"ACS Infect Dis"},{"key":"10.1016\/j.jve.2023.100327_bib81","doi-asserted-by":"crossref","DOI":"10.1016\/j.antiviral.2022.105389","article-title":"Punicalagin as an allosteric NSP13 helicase inhibitor potently suppresses SARS-CoV-2 replication in vitro","volume":"206","author":"Lu","year":"2022","journal-title":"Antivir Res"},{"issue":"21","key":"10.1016\/j.jve.2023.100327_bib82","doi-asserted-by":"crossref","first-page":"7522","DOI":"10.3390\/molecules27217522","article-title":"Targeting SARS-CoV-2 nsp13 helicase and assessment of druggability pockets: identification of two potent inhibitors by a multi-site in silico drug repurposing approach","volume":"27","author":"Romeo","year":"2022","journal-title":"Molecules"},{"key":"10.1016\/j.jve.2023.100327_bib83","doi-asserted-by":"crossref","first-page":"39578","DOI":"10.1074\/jbc.C300328200","article-title":"The severe acute respiratory syndrome (SARS) coronavirus NTPase\/helicase belongs to a distinct class of 5' to 3' viral helicases","volume":"278","author":"Tanner","year":"2003","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jve.2023.100327_bib84","doi-asserted-by":"crossref","first-page":"5619","DOI":"10.1128\/JVI.78.11.5619-5632.2004","article-title":"Multiple enzymatic activities associated with severe acute respiratory syndrome coronavirus helicase","volume":"78","author":"Ivanov","year":"2004","journal-title":"J Virol"},{"key":"10.1016\/j.jve.2023.100327_bib85","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0036521","article-title":"Mechanism of nucleic acid unwinding by SARS-CoV helicase","volume":"7","author":"Adedeji","year":"2012","journal-title":"PLoS One"},{"issue":"Database issue","key":"10.1016\/j.jve.2023.100327_bib86","doi-asserted-by":"crossref","first-page":"D901","DOI":"10.1093\/nar\/gkm958","article-title":"DrugBank: a knowledgebase for drugs, drug actions and drug targets","volume":"36","author":"Wishart","year":"2008","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.jve.2023.100327_bib87","doi-asserted-by":"crossref","first-page":"3037","DOI":"10.3390\/molecules14083037","article-title":"Plant-derived antimalarial agents: new leads and efficient phytomedicines. Part II. Non-alkaloidal natural products","volume":"14","author":"Batista","year":"2009","journal-title":"Molecules"},{"key":"10.1016\/j.jve.2023.100327_bib88","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/bs.podrm.2020.07.005","article-title":"Validation of in-vitro bioassay methods: application in herbal drug research","volume":"46","author":"Indrayanto","year":"2021","journal-title":"Profiles Drug Subst Excipients Relat Methodol"},{"key":"10.1016\/j.jve.2023.100327_bib89","doi-asserted-by":"crossref","first-page":"41","DOI":"10.24875\/AIDSRev.22000001","article-title":"Oral antivirals for the prevention and treatment of SARS-CoV-2 infection","volume":"24","author":"Soriano","year":"2022","journal-title":"AIDS Rev"},{"key":"10.1016\/j.jve.2023.100327_bib90","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1021\/acs.jproteome.0c00526","article-title":"Overview of targets and potential drugs of SARS-CoV-2 according to the viral replication","volume":"20","author":"Zhang","year":"2021","journal-title":"J Proteome Res"},{"key":"10.1016\/j.jve.2023.100327_bib91","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.3390\/ijms20061375","article-title":"The light and dark sides of virtual screening: what is there to know?","volume":"20","author":"Gimeno","year":"2019","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.jve.2023.100327_bib92","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.3389\/fphar.2018.01275","article-title":"QSAR-based virtual screening: advances and applications in drug discovery","volume":"9","author":"Neves","year":"2018","journal-title":"Front Pharmacol"},{"key":"10.1016\/j.jve.2023.100327_bib93","doi-asserted-by":"crossref","first-page":"3647","DOI":"10.1039\/D0CS01118E","article-title":"Antiviral drug discovery: preparing for the next pandemic","volume":"50","author":"Adamson","year":"2021","journal-title":"Chem Soc Rev"},{"key":"10.1016\/j.jve.2023.100327_bib94","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1038\/nprot.2014.112","article-title":"Real-time fluorescence assays to monitor duplex unwinding and ATPase activities of helicases","volume":"9","author":"\u00d6ze\u015f","year":"2014","journal-title":"Nat Protoc"},{"key":"10.1016\/j.jve.2023.100327_bib95","series-title":"Clinical Enzymology. InClinical Biochemistry of Domestic Animals","first-page":"303","author":"Kramer","year":"1997"},{"key":"10.1016\/j.jve.2023.100327_bib96","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/B978-0-12-814685-9.00014-2","article-title":"Assaying the activity of helicases: an overview","author":"Mojumdar","year":"2019","journal-title":"Helicases from All Domains of Life"},{"key":"10.1016\/j.jve.2023.100327_bib97","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1002\/bkcs.12229","article-title":"Analysis of nucleoside triphosphate hydrolysis by Middle East respiratory syndrome coronavirus helicase","volume":"42","author":"Woo","year":"2021","journal-title":"Bull Kor Chem Soc"},{"key":"10.1016\/j.jve.2023.100327_bib98","doi-asserted-by":"crossref","first-page":"1724","DOI":"10.5012\/bkcs.2009.30.8.1724","article-title":"ATP hydrolysis analysis of severe acute respiratory syndrome (SARS) coronavirus helicase","volume":"30","author":"Lee","year":"2009","journal-title":"Bull Kor Chem Soc"},{"key":"10.1016\/j.jve.2023.100327_bib99","doi-asserted-by":"crossref","first-page":"6334","DOI":"10.1073\/pnas.77.11.6334","article-title":"Ligand substitution reactions of metallothioneins with EDTA and apo-carbonic anhydrase","volume":"77","author":"Li","year":"1980","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jve.2023.100327_bib100","doi-asserted-by":"crossref","first-page":"3478","DOI":"10.1073\/pnas.95.7.3478","article-title":"Thiolate ligands in metallothionein confer redox activity on zinc clusters","volume":"95","author":"Maret","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jve.2023.100327_bib101","doi-asserted-by":"crossref","first-page":"29094","DOI":"10.1074\/jbc.274.41.29094","article-title":"Interactions of bismuth complexes with metallothionein(II)","volume":"274","author":"Sun","year":"1999","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jve.2023.100327_bib102","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.bbrc.2014.09.113","article-title":"Suramin inhibits helicase activity of NS3 protein of dengue virus in a fluorescence-based high throughput assay format","volume":"453","author":"Basavannacharya","year":"2014","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.jve.2023.100327_bib103","doi-asserted-by":"crossref","first-page":"E2011","DOI":"10.1073\/pnas.1601089113","article-title":"Suramin inhibits cullin-RING E3 ubiquitin ligases","volume":"113","author":"Wu","year":"2016","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jve.2023.100327_bib104","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0091765","article-title":"Structural bases of norovirus RNA dependent RNA polymerase inhibition by novel suramin-related compounds","volume":"9","author":"Croci","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.jve.2023.100327_bib105","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.jmb.2012.03.008","article-title":"Structure-based inhibition of Norovirus RNA-dependent RNA polymerases","volume":"419","author":"Mastrangelo","year":"2012","journal-title":"J Mol Biol"},{"key":"10.1016\/j.jve.2023.100327_bib106","article-title":"Assay interference by aggregation","author":"Auld","year":"2017","journal-title":"Assay Guidance Manual"},{"key":"10.1016\/j.jve.2023.100327_bib107","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.nantod.2018.02.011","article-title":"Colloidal aggregation: from screening nuisance to formulation nuance","volume":"19","author":"Ganesh","year":"2018","journal-title":"Nano Today"},{"key":"10.1016\/j.jve.2023.100327_bib108","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00687-21","article-title":"Construction of a noninfectious SARS-CoV-2 replicon for antiviral-drug testing and gene function studies","volume":"95","author":"Nguyen","year":"2021","journal-title":"J Virol"},{"key":"10.1016\/j.jve.2023.100327_bib109","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/B978-0-12-820267-8.00038-8","article-title":"Medicinal plants as promising source of natural antiviral substances against Zika virus","author":"Haddad","year":"2021","journal-title":"InZika Virus Impact, Diagnosis, Control, and Models"},{"key":"10.1016\/j.jve.2023.100327_bib110","doi-asserted-by":"crossref","first-page":"1546","DOI":"10.1016\/j.jiph.2022.11.016","article-title":"The efficacy of Zafirlukast as a SARS-CoV-2 helicase inhibitor in adult patients with moderate COVID-19 Pneumonia (pilot randomized clinical trial)","volume":"15","author":"Ghobain","year":"2022","journal-title":"J Infect Public Health"},{"key":"10.1016\/j.jve.2023.100327_bib111","doi-asserted-by":"crossref","DOI":"10.3389\/fchem.2022.1062352","article-title":"Therapeutic potential of compounds targeting SARS-CoV-2 helicase","volume":"10","author":"Halma","year":"2022","journal-title":"Front Chem"}],"container-title":["Journal of Virus Eradication"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2055664023000134?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2055664023000134?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T20:12:10Z","timestamp":1761855130000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2055664023000134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":111,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["S2055664023000134"],"URL":"https:\/\/doi.org\/10.1016\/j.jve.2023.100327","relation":{},"ISSN":["2055-6640"],"issn-type":[{"value":"2055-6640","type":"print"}],"subject":[],"published":{"date-parts":[[2023,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Coronaviruses SARS-CoV, MERS-CoV, and SARS-CoV-2 helicase inhibitors: a systematic review of in vitro studies","name":"articletitle","label":"Article Title"},{"value":"Journal of Virus Eradication","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jve.2023.100327","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Author. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"100327"}}