{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:48:42Z","timestamp":1773287322242,"version":"3.50.1"},"reference-count":52,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2004,5,1]],"date-time":"2004-05-01T00:00:00Z","timestamp":1083369600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2004,5]]},"DOI":"10.1016\/j.jviromet.2004.01.008","type":"journal-article","created":{"date-parts":[[2004,3,12]],"date-time":"2004-03-12T10:20:40Z","timestamp":1079086840000},"page":"169-177","source":"Crossref","is-referenced-by-count":19,"title":["Development of a biotin mimic tagged ScFv antibody against western equine encephalitis virus: bacterial expression and refolding"],"prefix":"10.1016","volume":"117","author":[{"given":"D","family":"Das","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Kriangkum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.P","family":"Nagata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.E","family":"Fulton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.R","family":"Suresh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2004.01.008_BIB1","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1006\/prep.1998.0996","article-title":"Refolding, purification, and characterization of a loop deletion mutant of human Bcl-2 from inclusion bodies","volume":"15","author":"Anderson","year":"1999","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB2","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1515\/BC.2001.197","article-title":"A recombinant scFv\/streptavidin-binding peptide fusion protein for the quantitative determination of the scorpion venom neurotoxin AahI","volume":"382","author":"Aubrey","year":"2001","journal-title":"Biol. Chem."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB3","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/0022-1759(95)00149-5","article-title":"Baculovirus expression of a functional single-chain immunoglobulin and its IL-2 fusion protein","volume":"186","author":"Bei","year":"1995","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jviromet.2004.01.008_BIB4","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1126\/science.3285471","article-title":"Escherichia coli secretion of an active chimeric antibody fragment","volume":"240","author":"Better","year":"1988","journal-title":"Science"},{"key":"10.1016\/j.jviromet.2004.01.008_BIB5","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1126\/science.3140379","article-title":"Single-chain antigen-binding proteins","volume":"242","author":"Bird","year":"1988","journal-title":"Science"},{"issue":"3","key":"10.1016\/j.jviromet.2004.01.008_BIB6","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1006\/prep.2001.1589","article-title":"Soluble expression of a functional recombinant cytolytic immunotoxin in insect cells","volume":"24","author":"Choo","year":"2002","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB7","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1038\/nbt0291-165","article-title":"Single chain antibody (SCA) encoding genes. One step construction and expression in eukaryotic cells","volume":"9","author":"Davis","year":"1991","journal-title":"Biotechnology"},{"key":"10.1016\/j.jviromet.2004.01.008_BIB8","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1126\/science.2143033","article-title":"Random peptide libraries: a source of specific protein binding molecules","volume":"249","author":"Devlin","year":"1990","journal-title":"Science"},{"key":"10.1016\/j.jviromet.2004.01.008_BIB9","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1038\/nbt0994-890","article-title":"Mammalian cell expression of single-chain Fv (SFv) antibody proteins and their C-terminal fusion with interleukin-2 and other effector domains","volume":"12","author":"Dorai","year":"1994","journal-title":"Biotechnology"},{"issue":"1","key":"10.1016\/j.jviromet.2004.01.008_BIB10","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1023\/A:1023902407855","article-title":"Expression of anti human IL-4 and IL-6 scFvs in transgenic tobacco plants","volume":"52","author":"Ehsani","year":"2003","journal-title":"Plant Mol. Biol."},{"issue":"1\u20133","key":"10.1016\/j.jviromet.2004.01.008_BIB11","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0014-5793(03)00871-8","article-title":"Anti-herbicide single-chain antibody expression confers herbicide tolerance in transgenic plants","volume":"550","author":"Eto","year":"2003","journal-title":"FEBS Lett."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB12","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1002\/psc.310010402","article-title":"Identification of avidin and streptavidin binding motifs among peptides selected from a synthetic peptide library consisting solely of D-amino acids","volume":"1","author":"Gissel","year":"1995","journal-title":"J. Pept. Sci."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB13","doi-asserted-by":"crossref","unstructured":"Gurkan, C., Symeonides, S.N., Ellar, D.J., 2003. High-level production in Pichia pastoris of an anti-p185 HER-2 scFv using an alternative secretion expression vector. Biotechnol. Appl. Biochem 9 September (Epub ahead of print).","DOI":"10.1042\/BA20030096"},{"issue":"1\u20132","key":"10.1016\/j.jviromet.2004.01.008_BIB14","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0022-1759(03)00100-5","article-title":"Isolation and expression of recombinant antibody fragments to the biological warfare pathogen Brucella melitensis","volume":"276","author":"Hayhurst","year":"2003","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jviromet.2004.01.008_BIB15","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1002\/(SICI)1097-0290(19970505)54:3<221::AID-BIT3>3.0.CO;2-H","article-title":"Oxidative renaturation of lysozyme at high concentration","volume":"54","author":"Hevehan","year":"1997","journal-title":"Biotechnol. Bioeng."},{"issue":"1","key":"10.1016\/j.jviromet.2004.01.008_BIB16","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1038\/nm0103-129","article-title":"Engineered antibodies","volume":"9","author":"Hudson","year":"2003","journal-title":"Nat. Med."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB17","doi-asserted-by":"crossref","first-page":"5879","DOI":"10.1073\/pnas.85.16.5879","article-title":"Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli","volume":"85","author":"Huston","year":"1988","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB18","unstructured":"Johnston, R.E., Peters, C.J., 1996. Alphavirus. In: Fields, B.N., Knipe, D.M., Howley, P.M. (Eds.), Fields Virology, third ed. Raven Publishers, Philadelphia, PA, pp. 843\u2013898."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB19","doi-asserted-by":"crossref","first-page":"26267","DOI":"10.1016\/S0021-9258(18)47189-X","article-title":"Mammalian expression and secretion of functional single-chain Fv molecules","volume":"269","author":"Jost","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB20","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1093\/protein\/8.1.81","article-title":"Engineered turns of a recombinant antibody improve its in vivo folding","volume":"8","author":"Knappik","year":"1995","journal-title":"Protein Eng."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB21","doi-asserted-by":"crossref","first-page":"2490","DOI":"10.1128\/AEM.64.7.2490-2496.1998","article-title":"Construction and expression of a bifunctional single-chain antibody against Bacillus cereus p6ores","volume":"64","author":"Koo","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"issue":"17\u201318","key":"10.1016\/j.jviromet.2004.01.008_BIB22","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1016\/0161-5890(95)00105-0","article-title":"Correct disulfide pairing and efficient refolding of detergent-solublilized single chain Fv proteins from bacterial inclusion bodies","volume":"32","author":"Kurucz","year":"1995","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB23","doi-asserted-by":"crossref","first-page":"7467","DOI":"10.1016\/S0021-9258(20)79727-9","article-title":"Renaturation of enzymes after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate","volume":"255","author":"Lacks","year":"1980","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB24","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"277","author":"Laemmli","year":"1970","journal-title":"Nat. (Lond.)"},{"key":"10.1016\/j.jviromet.2004.01.008_BIB25","doi-asserted-by":"crossref","first-page":"4379","DOI":"10.1128\/jb.169.9.4379-4383.1987","article-title":"Characterisation of the Erwinia carotovora pelB gene and its product pectate lyase","volume":"169","author":"Lei","year":"1987","journal-title":"J. Bacteriol."},{"issue":"1","key":"10.1016\/j.jviromet.2004.01.008_BIB26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1089\/027245700315743","article-title":"Construction and characterization of a novel recombinant single-chain variable fragment antibody against Western Equine Encephalitis virus","volume":"19","author":"Long","year":"2000","journal-title":"Hybridoma"},{"key":"10.1016\/j.jviromet.2004.01.008_BIB27","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s002840010206","article-title":"Purification, refolding of hybrid hIFN(-Kringle 5 expressed in Escherichia coli","volume":"42","author":"Lu","year":"2001","journal-title":"Curr. Microbiol."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB28","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0168-1656(98)00094-7","article-title":"Expression of a fusion protein of scFv-biotin mimetic peptide for immunoassay","volume":"65","author":"Luo","year":"1998","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB29","doi-asserted-by":"crossref","first-page":"5302","DOI":"10.1016\/S0021-9258(18)53533-X","article-title":"Characterization of single-chain antibody (ScFv)-toxin fusion proteins produced in vitro in rabbit reticulocyte lysate","volume":"268","author":"Nicholls","year":"1993","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB30","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s00253-002-1190-6","article-title":"Identification of factors impeding the production of a single-chain antibody fragment in Escherichia coli by comparing in vivo and in vitro expression","volume":"2","author":"Oelschlaeger","year":"2003","journal-title":"Appl. Microbial. Biotechnol."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB31","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/0014-5793(95)00262-8","article-title":"Novel avidin and streptavidin binding sequences found in synthetic peptide libraries","volume":"362","author":"Ostergaard","year":"1995","journal-title":"FEBS Lett."},{"issue":"1","key":"10.1016\/j.jviromet.2004.01.008_BIB32","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1023\/A:1013967705337","article-title":"Expression of a single-chain Fv antibody fragment specific for the hepatitis B surface antigen in transgenic tobacco plants","volume":"11","author":"Ramirez","year":"2002","journal-title":"Transgenic Res."},{"issue":"2","key":"10.1016\/j.jviromet.2004.01.008_BIB33","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1006\/prep.1999.1191","article-title":"Expression of functional recombinant antibody molecules in insect cell expression systems","volume":"18","author":"Reavy","year":"2000","journal-title":"Protein Expr. Purif."},{"issue":"3","key":"10.1016\/j.jviromet.2004.01.008_BIB34","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/nbt0395-255","article-title":"Generation of rabbit monoclonal antibody fragments from a combinational phage display library and their production in the yeast Pichia pastoris","volume":"13","author":"Ridder","year":"1995","journal-title":"Biotechnology"},{"key":"10.1016\/j.jviromet.2004.01.008_BIB35","unstructured":"Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB36","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0168-1656(99)00036-X","article-title":"High cytoplasmic expression in E. coli, purification, and in vitro refolding of a single chain Fv antibody fragment against the hepatitis B surface antigen","volume":"72","author":"Sanchez","year":"1999","journal-title":"J. Biotechnol."},{"issue":"25","key":"10.1016\/j.jviromet.2004.01.008_BIB37","doi-asserted-by":"crossref","first-page":"5015","DOI":"10.1021\/bi00827a028","article-title":"Formation of three-dimensional structure in proteins. I. Rapid nonenzyme reactivation of reduced lysozyme","volume":"9","author":"Saxena","year":"1970","journal-title":"Biochemistry"},{"key":"10.1016\/j.jviromet.2004.01.008_BIB38","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1006\/jmbi.1996.0061","article-title":"Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin","volume":"255","author":"Schmidt","year":"1996","journal-title":"J. Mol. Biol."},{"issue":"2","key":"10.1016\/j.jviromet.2004.01.008_BIB39","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S1046-5928(02)00706-4","article-title":"Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris","volume":"28","author":"Shi","year":"2003","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB40","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S1050-3862(99)00033-9","article-title":"Applications of a peptide ligand for streptavidin: the Strep-tag","volume":"16","author":"Skerra","year":"1999","journal-title":"Biomol. Eng."},{"issue":"4855","key":"10.1016\/j.jviromet.2004.01.008_BIB41","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1126\/science.3285470","article-title":"Assembly of a functional immunoglobulin Fv fragment in Escherichia coli","volume":"240","author":"Skerra","year":"1988","journal-title":"Science"},{"key":"10.1016\/j.jviromet.2004.01.008_BIB42","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S1046-5928(02)00709-X","article-title":"Secretion of functional anti-CD30-angiogenin immunotoxins into the supernatant of transfected 293T-cells","volume":"2","author":"Stocker","year":"2003","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB43","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/0076-6879(90)85008-C","article-title":"Use of T7 RNA polymerase to direct the expression of cloned genes","volume":"185","author":"Studier","year":"1990","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB44","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/0378-4347(94)00078-6","article-title":"Procedure for refolding and purification of recombinant proteins from Eschrichia coli inclusion bodies using a strong anion exchanger","volume":"656","author":"Suttnar","year":"1994","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.jviromet.2004.01.008_BIB45","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1006\/prep.1998.1017","article-title":"Affinity purification of recombinant interferon-alpha on a mimetic ligand absorbant","volume":"15","author":"Swaminathan","year":"1999","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB46","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.jviromet.2004.01.008_BIB47","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0022-1759(98)00077-5","article-title":"Antibody engineering: comparison of bacterial, yeast, insect and mammalian expression system","volume":"216","author":"Verma","year":"1998","journal-title":"J. Immunol. Methods"},{"issue":"6242","key":"10.1016\/j.jviromet.2004.01.008_BIB48","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1038\/341544a0","article-title":"Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli","volume":"341","author":"Ward","year":"1989","journal-title":"Nature"},{"key":"10.1016\/j.jviromet.2004.01.008_BIB49","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1042\/0264-6021:3400345","article-title":"Oxidative refolding of recombinant prochymosin","volume":"340","author":"Wei","year":"1999","journal-title":"Biochem. J."},{"issue":"4","key":"10.1016\/j.jviromet.2004.01.008_BIB50","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1042\/bst0220940","article-title":"Antibody production in transgenic plants","volume":"22","author":"Whitelam","year":"1994","journal-title":"Biochem. Soc. Trans."},{"issue":"4","key":"10.1016\/j.jviromet.2004.01.008_BIB51","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1089\/hyb.1999.18.315","article-title":"A single chain Fv specific against western equine encephalitis virus","volume":"18","author":"Xu","year":"1999","journal-title":"Hybridoma"},{"issue":"5","key":"10.1016\/j.jviromet.2004.01.008_BIB52","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1093\/jb\/mvg074","article-title":"Production of a functional catalytic antibody ScFv-NusA fusion protein in bacterial cytoplasm","volume":"133","author":"Zheng","year":"2003","journal-title":"J. Biochem. (Tokyo)"}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093404000102?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093404000102?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T02:55:37Z","timestamp":1623725737000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093404000102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,5]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,5]]}},"alternative-id":["S0166093404000102"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2004.01.008","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2004,5]]}}}