{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T13:36:58Z","timestamp":1791812218440,"version":"4.3.4"},"reference-count":31,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2004,12,1]],"date-time":"2004-12-01T00:00:00Z","timestamp":1101859200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2004,12]]},"DOI":"10.1016\/j.jviromet.2004.08.017","type":"journal-article","created":{"date-parts":[[2004,10,20]],"date-time":"2004-10-20T05:40:02Z","timestamp":1098250802000},"page":"131-139","source":"Crossref","is-referenced-by-count":261,"title":["Optimized large-scale production of high titer lentivirus vector pseudotypes"],"prefix":"10.1016","volume":"122","author":[{"given":"Miguel","family":"Sena-Esteves","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica C.","family":"Tebbets","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Steffens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Crombleholme","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan W.","family":"Flake","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2004.08.017_bib1","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1038\/sj.gt.3302094","article-title":"Optimized lentiviral vector production and purification procedure prevents immune response after transduction of mouse brain","volume":"10","author":"Baekelandt","year":"2003","journal-title":"Gene Ther."},{"key":"10.1016\/j.jviromet.2004.08.017_bib2","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1128\/JVI.75.2.1061-1064.2001","article-title":"Recombinant expression of lymphocytic choriomeningitis virus strain WE glycoproteins: a single amino acid makes the difference","volume":"75","author":"Beyer","year":"2001","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2004.08.017_bib3","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1128\/JVI.76.3.1488-1495.2002","article-title":"Oncoretrovirus and lentivirus vectors pseudotyped with lymphocytic choriomeningitis virus glycoprotein: generation, concentration, and broad host range","volume":"76","author":"Beyer","year":"2002","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2004.08.017_bib4","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1006\/mthe.2001.0431","article-title":"Production and neurotropism of lentivirus vectors pseudotyped with lyssavirus envelope glycoproteins","volume":"4","author":"Desmaris","year":"2001","journal-title":"Mol. Ther."},{"key":"10.1016\/j.jviromet.2004.08.017_bib5","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1006\/mthe.2002.0690","article-title":"Five recombinant simian immunodeficiency virus pseudotypes lead to exclusive transduction of retinal pigmented epithelium in rat","volume":"6","author":"Duisit","year":"2002","journal-title":"Mol. Ther."},{"key":"10.1016\/j.jviromet.2004.08.017_bib6","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1038\/76095","article-title":"Gene transfer by lentiviral vectors is limited by nuclear translocation and rescued by HIV-1 pol sequences","volume":"25","author":"Follenzi","year":"2000","journal-title":"Nat. Genet."},{"key":"10.1016\/j.jviromet.2004.08.017_bib7","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1128\/JVI.73.3.1828-1834.1999","article-title":"Requirements for efficient production and transduction of human immunodeficiency virus type 1-based vectors","volume":"73","author":"Gasmi","year":"1999","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2004.08.017_bib8","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1093\/nar\/18.4.937","article-title":"Intervening sequences increase efficiency of RNA 3\u2032 processing and accumulation of cytoplasmic RNA","volume":"18","author":"Huang","year":"1990","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jviromet.2004.08.017_bib9","doi-asserted-by":"crossref","first-page":"9378","DOI":"10.1128\/JVI.76.18.9378-9388.2002","article-title":"In vivo gene transfer using a nonprimate lentiviral vector pseudotyped with Ross River Virus glycoproteins","volume":"76","author":"Kang","year":"2002","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2004.08.017_bib10","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1089\/104303403322319372","article-title":"Comparison of transfection conditions for a lentivirus vector produced in large volumes","volume":"14","author":"Karolewski","year":"2003","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.jviromet.2004.08.017_bib11","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/85664","article-title":"Filovirus-pseudotyped lentiviral vector can efficiently and stably transduce airway epithelia in vivo","volume":"19","author":"Kobinger","year":"2001","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.jviromet.2004.08.017_bib12","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1006\/mthe.2002.0681","article-title":"Efficient transduction of liver and muscle after in utero injection of lentiviral vectors with different pseudotypes","volume":"6","author":"MacKenzie","year":"2002","journal-title":"Mol. Ther."},{"key":"10.1016\/j.jviromet.2004.08.017_bib13","doi-asserted-by":"crossref","first-page":"2109","DOI":"10.1093\/hmg\/10.19.2109","article-title":"Rabies virus glycoprotein pseudotyping of lentiviral vectors enables retrograde axonal transport and access to the nervous system after peripheral delivery","volume":"10","author":"Mazarakis","year":"2001","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.jviromet.2004.08.017_bib14","doi-asserted-by":"crossref","first-page":"8150","DOI":"10.1128\/JVI.72.10.8150-8157.1998","article-title":"Development of a self-inactivating lentivirus vector","volume":"72","author":"Miyoshi","year":"1998","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2004.08.017_bib15","doi-asserted-by":"crossref","first-page":"8873","DOI":"10.1128\/JVI.72.11.8873-8883.1998","article-title":"High-titer human immunodeficiency virus type 1-based vector systems for gene delivery into nondividing cells","volume":"72","author":"Mochizuki","year":"1998","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2004.08.017_bib16","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.1038\/sj.gt.3301552","article-title":"Organ distribution of gene expression after intravenous infusion of targeted and untargeted lentiviral vectors","volume":"8","author":"Peng","year":"2001","journal-title":"Gene Ther."},{"key":"10.1016\/j.jviromet.2004.08.017_bib17","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1002\/1521-2254(2000)9999:9999<::AID-JGM159>3.0.CO;2-9","article-title":"Concentration of viral vectors by co-precipitation with calcium phosphate","volume":"3","author":"Pham","year":"2001","journal-title":"J. Gene Med."},{"key":"10.1016\/j.jviromet.2004.08.017_bib18","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1038\/sj.gt.3301188","article-title":"Production and concentration of pseudotyped HIV-1-based gene transfer vectors","volume":"7","author":"Reiser","year":"2000","journal-title":"Gene Ther."},{"key":"10.1016\/j.jviromet.2004.08.017_bib19","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1006\/jmcc.1999.1035","article-title":"A high-titer lentiviral production system mediates efficient transduction of differentiated cells including beating cardiac myocytes","volume":"31","author":"Sakoda","year":"1999","journal-title":"J. Mol. Cell Cardiol."},{"key":"10.1016\/j.jviromet.2004.08.017_bib20","doi-asserted-by":"crossref","first-page":"1708","DOI":"10.1038\/sj.gt.3301848","article-title":"Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography","volume":"9","author":"Scherr","year":"2002","journal-title":"Gene Ther."},{"key":"10.1016\/j.jviromet.2004.08.017_bib21","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1093\/nar\/23.4.628","article-title":"A transient three-plasmid expression system for the production of high titer retroviral vectors","volume":"23","author":"Soneoka","year":"1995","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jviromet.2004.08.017_bib22","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1006\/viro.2000.0394","article-title":"Lentiviral vectors pseudotyped with envelope glycoproteins derived from gibbon ape leukemia virus and murine leukemia virus 10A1","volume":"273","author":"Stitz","year":"2000","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2004.08.017_bib23","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1182\/blood-2002-07-2224","article-title":"IL-7 surface-engineered lentiviral vectors promote survival and efficient gene transfer in resting primary T lymphocytes","volume":"101","author":"Verhoeyen","year":"2003","journal-title":"Blood"},{"key":"10.1016\/j.jviromet.2004.08.017_bib24","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1006\/mthe.2002.0584","article-title":"Targeted transduction patterns in the mouse brain by lentivirus vectors pseudotyped with VSV, Ebola, Mokola, LCMV, or MuLV envelope proteins","volume":"5","author":"Watson","year":"2002","journal-title":"Mol. Ther."},{"key":"10.1016\/j.jviromet.2004.08.017_bib25","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1242\/jcs.108.2.441","article-title":"Real-time analysis of the transcriptional regulation of HIV and hCMV promoters in single mammalian cells","volume":"108","author":"White","year":"1995","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.jviromet.2004.08.017_bib26","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.ymthe.2003.09.017","article-title":"Transduction patterns of pseudotyped lentiviral vectors in the nervous system","volume":"9","author":"Wong","year":"2004","journal-title":"Mol. Ther."},{"key":"10.1016\/j.jviromet.2004.08.017_bib27","doi-asserted-by":"crossref","DOI":"10.2144\/03345dd04","article-title":"Lentivirus vector purification using anion exchange HPLC leads to improved gene transfer","volume":"34","author":"Yamada","year":"2003","journal-title":"Biotechniques"},{"key":"10.1016\/j.jviromet.2004.08.017_bib28","doi-asserted-by":"crossref","first-page":"9564","DOI":"10.1073\/pnas.91.20.9564","article-title":"A general method for the generation of high-titer, pantropic retroviral vectors: highly efficient infection of primary hepatocytes","volume":"91","author":"Yee","year":"1994","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.jviromet.2004.08.017_bib29","doi-asserted-by":"crossref","first-page":"2886","DOI":"10.1128\/JVI.73.4.2886-2892.1999","article-title":"Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors","volume":"73","author":"Zufferey","year":"1999","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2004.08.017_bib30","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1038\/nbt0997-871","article-title":"Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo","volume":"15","author":"Zufferey","year":"1997","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.jviromet.2004.08.017_bib31","series-title":"Current Protocol in Human Genetics","first-page":"12.10.1","article-title":"Production of high-titer lentiviral vectors","author":"Zufferey","year":"2000"}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016609340400237X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016609340400237X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,6,26]],"date-time":"2021-06-26T14:04:53Z","timestamp":1624716293000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S016609340400237X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["S016609340400237X"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2004.08.017","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2004,12]]}}}