{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T07:17:26Z","timestamp":1777533446373,"version":"3.51.4"},"reference-count":37,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2007,5,1]],"date-time":"2007-05-01T00:00:00Z","timestamp":1177977600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2007,5]]},"DOI":"10.1016\/j.jviromet.2006.12.005","type":"journal-article","created":{"date-parts":[[2007,1,25]],"date-time":"2007-01-25T15:04:55Z","timestamp":1169737495000},"page":"154-165","source":"Crossref","is-referenced-by-count":16,"title":["Orthopoxvirus pan-genomic DNA assay"],"prefix":"10.1016","volume":"141","author":[{"given":"Yu","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan L.","family":"Ropp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inger K.","family":"Damon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph J.","family":"Esposito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2006.12.005_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/viro.2001.1343","article-title":"The genome of camelpox virus","volume":"295","author":"Afonso","year":"2002","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2006.12.005_bib2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.mcp.2005.05.003","article-title":"Two-color multiplex assay for the identification of orthopox viruses with real-time LUX-PCR","volume":"19","author":"Aitichou","year":"2005","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.jviromet.2006.12.005_bib3","series-title":"Virus Taxonomy: Eighth Report of the International Committee on the Taxonomy of Viruses","first-page":"117","article-title":"Poxviridae","author":"Buller","year":"2005"},{"key":"10.1016\/j.jviromet.2006.12.005_bib4","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.virol.2005.05.030","article-title":"Virulence differences between monkeypox virus isolates from West Africa and the Congo basin","volume":"340","author":"Chen","year":"2005","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2006.12.005_bib5","doi-asserted-by":"crossref","first-page":"3801","DOI":"10.1093\/nar\/22.18.3801","article-title":"A simple procedure for optimising the polymerase chain reaction (PCR) using modified Taguchi methods","volume":"22","author":"Cobb","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jviromet.2006.12.005_bib6","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1056\/NEJMoa031467","article-title":"Tanapox infection in a college student","volume":"350","author":"Dhar","year":"2004","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jviromet.2006.12.005_bib7","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/BF00125682","article-title":"Vaccinia virus recombinants expressing rabiesvirus glycoprotein protect against rabies","volume":"1","author":"Esposito","year":"1987","journal-title":"Virus Genes"},{"key":"10.1016\/j.jviromet.2006.12.005_bib8","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/0166-0934(81)90036-7","article-title":"The preparation of orthopoxvirus DNA","volume":"2","author":"Esposito","year":"1981","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2006.12.005_bib9","series-title":"Fields Virology","first-page":"2885","article-title":"Poxviruses","author":"Esposito","year":"2001"},{"key":"10.1016\/j.jviromet.2006.12.005_bib10","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/0042-6822(85)90111-4","article-title":"Orthopoxvirus DNA: a comparison of restriction profiles and maps","volume":"143","author":"Esposito","year":"1985","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2006.12.005_bib11","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/0042-6822(78)90039-9","article-title":"Orthopoxvirus DNA: strain differentiation by electrophoresis of restriction endonuclease fragmented virion DNA","volume":"89","author":"Esposito","year":"1978","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2006.12.005_bib12","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1126\/science.1125134","article-title":"Genome sequence diversity and clues to the evolution of variola (smallpox) virus","volume":"313","author":"Esposito","year":"2006","journal-title":"Science"},{"key":"10.1016\/j.jviromet.2006.12.005_bib13","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/0042-6822(90)90294-2","article-title":"The complete DNA sequence of vaccinia virus","volume":"179","author":"Goebel","year":"1990","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2006.12.005_bib14","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1099\/0022-1317-83-4-855","article-title":"The sequence of camelpox virus shows it is most closely related to variola virus, the cause of smallpox","volume":"83","author":"Gubser","year":"2002","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2006.12.005_bib15","doi-asserted-by":"crossref","first-page":"3835","DOI":"10.1128\/JCM.41.8.3835-3839.2003","article-title":"Real-time PCR assay to detect smallpox virus","volume":"41","author":"Ibrahim","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2006.12.005_bib16","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1006\/viro.1993.1494","article-title":"An update on the vaccinia virus genome","volume":"196","author":"Johnson","year":"1993","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2006.12.005_bib17","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/0042-6822(92)91228-M","article-title":"Further analyses of the orthopoxviruses volepox virus and raccoon poxvirus","volume":"190","author":"Knight","year":"1992","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2006.12.005_bib18","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S1084-8592(97)80032-X","article-title":"Optimization of the isolation and amplification of RNA from formalin-fixed, paraffin-embedded tissue: The Armed Forces Institute of Pathology experience and literature review","volume":"2","author":"Krafft","year":"1997","journal-title":"Mol. Diagn."},{"key":"10.1016\/j.jviromet.2006.12.005_bib19","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1128\/JCM.42.2.601-609.2004","article-title":"Smallpox and pan-orthopox virus detection by real-time 3\u2032-minor groove binder TaqMan assays on the Roche LightCycler and the Cepheid smart Cycler platforms","volume":"42","author":"Kulesh","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2006.12.005_bib20","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S0166-0934(03)00193-9","article-title":"Detection and discrimination of orthopoxviruses using microarrays of immobilized oligonucleotides","volume":"112","author":"Laassri","year":"2003","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2006.12.005_bib21","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.jcv.2006.03.012","article-title":"Detection of monkeypox virus with real-time PCR assays","volume":"36","author":"Li","year":"2006","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jviromet.2006.12.005_bib22","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1099\/vir.0.81215-0","article-title":"A tale of two clades: monkeypox viruses","volume":"86","author":"Likos","year":"2005","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2006.12.005_bib23","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1099\/0022-1317-45-3-683","article-title":"Conservation and variation in Orthopoxvirus genome structure","volume":"45","author":"Mackett","year":"1979","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2006.12.005_bib24","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1038\/366748a0","article-title":"Potential virulence determinants in terminal regions of variola smallpox virus genome","volume":"366","author":"Massung","year":"1993","journal-title":"Nature"},{"key":"10.1016\/j.jviromet.2006.12.005_bib25","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1006\/viro.1994.1288","article-title":"Analysis of the complete genome of smallpox variola major virus strain Bangladesh-1975","volume":"201","author":"Massung","year":"1994","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2006.12.005_bib26","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/0378-1135(93)90058-F","article-title":"Differentiation of species of the genus Orthopoxvirus in a dot blot assay using digoxigenin-labeled DNA-probes","volume":"34","author":"Meyer","year":"1993","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.jviromet.2006.12.005_bib27","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0166-0934(96)02155-6","article-title":"Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses","volume":"64","author":"Meyer","year":"1997","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2006.12.005_bib28","series-title":"Fields Virology","first-page":"2849","article-title":"Poxviridae: the viruses and their replication","author":"Moss","year":"2001"},{"key":"10.1016\/j.jviromet.2006.12.005_bib29","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1128\/JCM.42.5.1940-1946.2004","article-title":"Real-time PCR system for detection of orthopoxviruses and simultaneous identification of smallpox virus","volume":"42","author":"Olson","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2006.12.005_bib30","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1056\/NEJMoa032299","article-title":"The detection of monkeypox in humans in the Western Hemisphere","volume":"350","author":"Reed","year":"2004","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jviromet.2006.12.005_bib31","doi-asserted-by":"crossref","first-page":"2069","DOI":"10.1128\/JCM.33.8.2069-2076.1995","article-title":"PCR strategy for identification and differentiation of smallpox and other orthopoxviruses","volume":"33","author":"Ropp","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2006.12.005_bib32","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1006\/viro.2002.1446","article-title":"Analysis of the monkeypox virus genome","volume":"297","author":"Shchelkunov","year":"2002","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2006.12.005_bib33","doi-asserted-by":"crossref","first-page":"4539","DOI":"10.1093\/nar\/12.11.4539","article-title":"Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-\/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product","volume":"12","author":"Sinha","year":"1984","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jviromet.2006.12.005_bib34","first-page":"180","article-title":"Design and preparation of oligonucleotide microarray for vaccinia virus detection","volume":"24","author":"Wang","year":"2004","journal-title":"Di Yi. Jun. Yi. Da. Xue. Xue. Bao."},{"key":"10.1016\/j.jviromet.2006.12.005_bib35","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1080\/09674845.2004.11732660","article-title":"An oligonucleotide microarray for the detection of vaccinia virus","volume":"61","author":"Wenli","year":"2004","journal-title":"Br. J. Biomed. Sci."},{"key":"10.1016\/j.jviromet.2006.12.005_bib36","first-page":"15","article-title":"Smallpox eradication: destruction of variola virus stocks","volume":"55","author":"World Health Assembly","year":"2002","journal-title":"Resolution WHA"},{"key":"10.1016\/j.jviromet.2006.12.005_bib37","first-page":"34","article-title":"Smallpox eradication: destruction of variola virus stocks","volume":"77","author":"World Health Organization","year":"2002","journal-title":"Wkly. Epidemiol. Rec."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093406004459?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093406004459?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T14:27:58Z","timestamp":1683728878000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093406004459"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,5]]}},"alternative-id":["S0166093406004459"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2006.12.005","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2007,5]]}}}