{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T14:31:27Z","timestamp":1771511487632,"version":"3.50.1"},"reference-count":45,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2007,12,1]],"date-time":"2007-12-01T00:00:00Z","timestamp":1196467200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2007,12]]},"DOI":"10.1016\/j.jviromet.2007.06.005","type":"journal-article","created":{"date-parts":[[2007,7,21]],"date-time":"2007-07-21T11:23:41Z","timestamp":1185017021000},"page":"86-95","source":"Crossref","is-referenced-by-count":31,"title":["A new method to identify cell types that support porcine circovirus type 2 replication in formalin-fixed, paraffin-embedded swine tissues"],"prefix":"10.1016","volume":"146","author":[{"given":"Eva","family":"P\u00e9rez-Mart\u00edn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert","family":"Rovira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Calsamiglia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette","family":"Mankertz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joaquim","family":"Segal\u00e9s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2007.06.005_bib1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1177\/104063870001200102","article-title":"Porcine circoviruses: a review","volume":"12","author":"Allan","year":"2000","journal-title":"J. Vet. Diagn. Invest."},{"key":"10.1016\/j.jviromet.2007.06.005_bib2","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jviromet.2007.06.005_bib3","series-title":"Proceedings of the International Pig Veterinary Society Congress","first-page":"62","article-title":"Does genetics or litter effect influence moratlity in PMWS","author":"Armstrong","year":"2004"},{"key":"10.1016\/j.jviromet.2007.06.005_bib4","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1177\/104063870101300301","article-title":"Postweaning multisystemic wasting syndrome induced after experimental inoculation of cesarean-derived, colostrum-deprived piglets with type 2 porcine circovirus","volume":"13","author":"Bolin","year":"2001","journal-title":"J. Vet. Diagn. Invest."},{"issue":"2","key":"10.1016\/j.jviromet.2007.06.005_bib5","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.jviromet.2004.08.014","article-title":"Quantitation of porcine circovirus type 2 isolated from serum\/plasma and tissue samples of healthy pigs and pigs with postweaning multisystemic wasting syndrome using a TaqMan-based real-time PCR","volume":"122","author":"Brunborg","year":"2004","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2007.06.005_bib6","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s705-002-8302-4","article-title":"Kinetics of porcine circovirus type 2 replication","volume":"147","author":"Cheung","year":"2002","journal-title":"Arch. Virol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib7","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0165-2427(01)00364-6","article-title":"Immunohistological study of the immune system cells in paraffin-embedded tissues of conventional pigs","volume":"82","author":"Chianini","year":"2001","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib8","series-title":"Proc. West Can. Assoc. Swine Pract.","first-page":"22","article-title":"Pathology of the post-weaning multisystemic wasting syndrome of pigs","author":"Clark","year":"1995"},{"key":"10.1016\/j.jviromet.2007.06.005_bib9","series-title":"Robbins Pathologic Basis of Disease","first-page":"50","article-title":"Acute and chronic inflammation","author":"Collins","year":"1999"},{"key":"10.1016\/j.jviromet.2007.06.005_bib10","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1007\/s00705-003-0280-9","article-title":"Pathogenesis of postweaning multisystemic wasting syndrome caused by porcine circovirus 2: an immune riddle","volume":"149","author":"Darwich","year":"2004","journal-title":"Arch. Virol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib11","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.virol.2005.08.021","article-title":"Analysis of the subcellular localization of the preoteins Rep, Rep\u2032 and Cap of porcine circovirus type 1","volume":"5","author":"Finsterbusch","year":"2005","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2007.06.005_bib12","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0165-2427(03)00087-4","article-title":"In vitro studies on the infection and replication of porcine circovirus type 2 in cells of the porcine immune system","volume":"94","author":"Gilpin","year":"2003","journal-title":"Vet. Immunol. Immunopathol."},{"issue":"2","key":"10.1016\/j.jviromet.2007.06.005_bib13","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1177\/104063870701900201","article-title":"A novel method for the detection of porcine circovirus type 2 replicative double stranded viral DNA and nonreplicative single stranded viral DNA in tissue sections","volume":"19","author":"Hamberg","year":"2007","journal-title":"J. Vet. Diagn. Invest."},{"key":"10.1016\/j.jviromet.2007.06.005_bib14","doi-asserted-by":"crossref","first-page":"5262","DOI":"10.1128\/JVI.72.6.5262-5267.1998","article-title":"Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs","volume":"72","author":"Hamel","year":"1998","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib15","series-title":"Proc. West. Can. Assoc. Swine Pract.","first-page":"21","article-title":"Post-weaning multisystemic wasting syndrome (PMWS): preliminary epidemiology and clinical presentation","author":"Harding","year":"1996"},{"key":"10.1016\/j.jviromet.2007.06.005_bib16","series-title":"Viral Pathogenesis","first-page":"35","article-title":"Localization of viral infections","author":"Holmes","year":"1997"},{"key":"10.1016\/j.jviromet.2007.06.005_bib17","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1053\/jcpa.1999.0337","article-title":"Reproduction of lesions of postweaning multisystemic wasting syndrome by infection of conventional pigs with porcine circovirus type 2 alone or in combination with porcine parvovirus","volume":"122","author":"Kennedy","year":"2000","journal-title":"J. Comp. Pathol."},{"issue":"2","key":"10.1016\/j.jviromet.2007.06.005_bib18","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1177\/104063870301500209","article-title":"Absence of evidence of porcine circovirus infection in piglets with congenital tremors","volume":"15","author":"Kennedy","year":"2003","journal-title":"J. Vet. Diagn. Invest."},{"key":"10.1016\/j.jviromet.2007.06.005_bib19","first-page":"77","article-title":"Cellular localization of porcine circovirus in postweaning pigs with chronic wasting disease","volume":"5","author":"Kiupel","year":"1999","journal-title":"Eur. J. Vet. Pathol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib20","series-title":"Proc. 4th International Symposium on Emerging and Re-emerging Pig Diseases","first-page":"143","article-title":"The pathogenesis of PCV-2-associated postweaning multisystemic wasting syndrome in swine","author":"Krakowka","year":"2003"},{"key":"10.1016\/j.jviromet.2007.06.005_bib21","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1354\/vp.37-3-254","article-title":"Viral wasting syndrome of swine: experimental reproduction of postweaning multisystemic wasting syndrome in gnotobiotic swine by coinfection with porcine circovirus 2 and porcine parvovirus","volume":"37","author":"Krakowka","year":"2000","journal-title":"Vet. Pathol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib22","doi-asserted-by":"crossref","first-page":"5065","DOI":"10.1128\/JVI.80.10.5065-5073.2006","article-title":"The ORF3 protein of porcine circovirus type 2 is involved in viral pathogenesis in vivo","volume":"80","author":"Liu","year":"2006","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib23","series-title":"Atlas of Clinical Hematology","author":"L\u00f6ffler","year":"2005"},{"key":"10.1016\/j.jviromet.2007.06.005_bib24","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.vetmic.2003.10.014","article-title":"Molecular biology of porcine circovirus: analyses of gene expression and viral replication","volume":"98","author":"Mankertz","year":"2004","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib25","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1007\/s00705-004-0444-2","article-title":"Replication kinetics of different porcine circovirus 2 strains in PK-15 cells, fetal cardiomyocytes and macrophages","volume":"150","author":"Meerts","year":"2005","journal-title":"Arch. Virol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib26","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0166-0934(99)00043-9","article-title":"A comparison of in situ hybridization and immunohistochemistry for the detection of a new porcine circovirus in formalin-fixed tissues from pigs with post-weaning multisystemic wasting syndrome (PMWS)","volume":"80","author":"McNeilly","year":"1999","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2007.06.005_bib27","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1177\/030098588602300616","article-title":"Characterization of cytoplasmic lysozyme immunoreactivity as a histiocytic marker in normal canine tissues","volume":"23","author":"Moore","year":"1986","journal-title":"Vet. Pathol."},{"issue":"1","key":"10.1016\/j.jviromet.2007.06.005_bib28","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jviromet.2003.12.007","article-title":"Comparison of porcine circovirus type 2 load in serum quantified by a real time PCR in postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome naturally affected pigs","volume":"117","author":"Olvera","year":"2004","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2007.06.005_bib29","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1099\/vir.0.79836-0","article-title":"In vitro and in vivo characterization of an infectious clone of a European strain of porcine circovirus type 2","volume":"85","author":"Roca","year":"2004","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib30","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1053\/jcpa.1998.0258","article-title":"Pathological, immunohistochemical and in situ hybridization studies of natural cases of postweaning multisystemic wasting syndrome (PMWS) in pigs","volume":"120","author":"Rosell","year":"1999","journal-title":"J. Comp. Pathol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib31","series-title":"Molecular Cloning a Laboratory Manual","author":"Sambrook","year":"2001"},{"issue":"1\u20132","key":"10.1016\/j.jviromet.2007.06.005_bib32","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0378-1135(03)00120-2","article-title":"Change of porcine circovirus 2 target cells in pigs during development from fetal to early postnatal life","volume":"95","author":"Sanchez","year":"2003","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib33","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1177\/104063870401600301","article-title":"Characteristics of porcine circovirus 2 replication in lymphoid organs of pigs inoculated in late-gestation or postnatally and possible relation to clinical and pathological outcome of infection","volume":"16","author":"Sanchez","year":"2004","journal-title":"J. Vet. Diagn. Invest."},{"key":"10.1016\/j.jviromet.2007.06.005_bib34","doi-asserted-by":"crossref","first-page":"5929","DOI":"10.1002\/j.1460-2075.1994.tb06938.x","article-title":"Productive HIV-1 infection of macrophages restricted to the cell fraction with proliferative capacity","volume":"13","author":"Schuitemaker","year":"1994","journal-title":"EMBO J."},{"key":"10.1016\/j.jviromet.2007.06.005_bib35","doi-asserted-by":"crossref","first-page":"277","DOI":"10.54846\/jshap\/351","article-title":"Update on postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome diagnostics","volume":"10","author":"Segal\u00e9s","year":"2002","journal-title":"J. Swine Health Prod."},{"key":"10.1016\/j.jviromet.2007.06.005_bib36","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1079\/AHR2005106","article-title":"Porcine circovirus diseases","volume":"6","author":"Segal\u00e9s","year":"2005","journal-title":"Anim. Health Res. Rev."},{"key":"10.1016\/j.jviromet.2007.06.005_bib37","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.vetmic.2003.10.007","article-title":"Immunosupression in postweaning multisystemic wasting syndrome affected pigs","volume":"98","author":"Segal\u00e9s","year":"2004","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib38","first-page":"125","article-title":"Diagnosis of swine infectious diseases using in situ hybridization","volume":"7","author":"Segal\u00e9s","year":"1999","journal-title":"Swine Health Prod."},{"key":"10.1016\/j.jviromet.2007.06.005_bib39","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1016\/0046-8177(93)90192-J","article-title":"Immunohistologic studies of Kikuchi's disease","volume":"24","author":"Sumiyoshi","year":"1993","journal-title":"Hum. Pathol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib40","first-page":"280","article-title":"Viral DNA from cells infected with porcine circovirus","volume":"270","author":"Tischer","year":"1988","journal-title":"Zentralbl. Bakteriol. Mikrobiol. Hyg."},{"key":"10.1016\/j.jviromet.2007.06.005_bib41","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/BF01310989","article-title":"Replication of porcine circovirus: induction by glucosamine and cell cycle dependence","volume":"96","author":"Tischer","year":"1987","journal-title":"Arch. Virol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib42","series-title":"Virus Taxonomy: Classification and Nomenclature of Viruses. Seventh Report of the International Committee on Taxonomy of Viruses","first-page":"299","article-title":"Family Circoviridae","author":"Todd","year":"2000"},{"key":"10.1016\/j.jviromet.2007.06.005_bib43","doi-asserted-by":"crossref","first-page":"13288","DOI":"10.1128\/JVI.77.24.13288-13300.2003","article-title":"Dendritic cells harbor infectious porcine circovirus type 2 in the absence of apparent cell modulation or replication of the virus","volume":"77","author":"Vincent","year":"2003","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib44","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.vetimm.2006.11.006","article-title":"Porcine circovirus type 2 (PCV2) distribution and replication in tissues and immune cells in early infected pigs","volume":"115","author":"Yu","year":"2007","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.jviromet.2007.06.005_bib45","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.vetmic.2007.02.021","article-title":"Quantification of PCV2 capsid transcript in peripheral blood mononuclear cells (PBMCs) in vitro","volume":"123","author":"Yu","year":"2007","journal-title":"Vet. Microbiol"}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093407002212?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093407002212?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,1,19]],"date-time":"2025-01-19T21:16:30Z","timestamp":1737321390000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093407002212"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,12]]},"references-count":45,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["S0166093407002212"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2007.06.005","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2007,12]]}}}