{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:22:03Z","timestamp":1792736523283,"version":"4.5.0"},"reference-count":14,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2008,6,1]],"date-time":"2008-06-01T00:00:00Z","timestamp":1212278400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2008,6]]},"DOI":"10.1016\/j.jviromet.2008.02.009","type":"journal-article","created":{"date-parts":[[2008,4,3]],"date-time":"2008-04-03T12:23:34Z","timestamp":1207225414000},"page":"14-20","source":"Crossref","is-referenced-by-count":31,"title":["A rapid, high-throughput vaccinia virus neutralization assay for testing smallpox vaccine efficacy based on detection of green fluorescent protein"],"prefix":"10.1016","volume":"150","author":[{"given":"Matthew C.","family":"Johnson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inger K.","family":"Damon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin L.","family":"Karem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2008.02.009_bib1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1083\/jcb.146.1.113","article-title":"Intracellular localization of proteasomal degradation of a viral antigen","volume":"146","author":"Ant\u00f3n","year":"1999","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.jviromet.2008.02.009_bib2","doi-asserted-by":"crossref","first-page":"3403","DOI":"10.1128\/MCB.5.12.3403","article-title":"Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques","volume":"5","author":"Chakrabarti","year":"1985","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.jviromet.2008.02.009_bib3","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1128\/CDLI.11.2.406-410.2004","article-title":"Neutralization assay using a modified vaccinia virus Ankara vector expressing the green fluorescent protein is a high-throughput method to monitor the humoral immune response against vaccinia virus","volume":"11","author":"Cosma","year":"2003","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.jviromet.2008.02.009_bib4","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0022-1759(98)00156-2","article-title":"Green fluorescent protein expressed by recombinant vaccinia virus permits early detection by flow cytometry","volume":"220","author":"Dominguez","year":"1998","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jviromet.2008.02.009_bib5","doi-asserted-by":"crossref","first-page":"10684","DOI":"10.1128\/JVI.77.19.10684-10688.2003","article-title":"Development and use of a vaccinia virus neutralization assay based on flow cytometric detection of green fluorescent protein","volume":"77","author":"Earl","year":"2003","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2008.02.009_bib6","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.jviromet.2005.05.027","article-title":"Development of a tissue-culture-based enzyme-immunoassay method for the quantitation of anti-vaccinia-neutralizing antibodies in human sera","volume":"130","author":"Eyal","year":"2005","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2008.02.009_bib7","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/S0092-1157(87)80013-6","article-title":"The effect of the virus-serum incubation period upon vaccinia virus serum neutralization titers","volume":"15","author":"Katz","year":"1987","journal-title":"J. Biol. Stand."},{"key":"10.1016\/j.jviromet.2008.02.009_bib8","doi-asserted-by":"crossref","first-page":"13454","DOI":"10.1128\/JVI.79.21.13454-13462.2005","article-title":"Combinations of polyclonal or monoclonal antibodies to proteins of the outer membranes of the two infectious forms of vaccinia virus protect mice against a lethal respiratory challenge","volume":"79","author":"Lustig","year":"2005","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2008.02.009_bib9","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1128\/JVI.49.3.857-864.1984","article-title":"General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes","volume":"49","author":"Mackett","year":"1984","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2008.02.009_bib10","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1086\/376557","article-title":"Development of a novel vaccinia-neutralization assay based on reporter-gene expression","volume":"188","author":"Manischewitz","year":"2003","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jviromet.2008.02.009_bib11","doi-asserted-by":"crossref","first-page":"3154","DOI":"10.1128\/JCM.41.7.3154-3157.2003","article-title":"Improved assay to detect neutralizing antibody following vaccination with diluted or undiluted vaccinia (Dryvax) vaccine","volume":"41","author":"Newman","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2008.02.009_bib12","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1038\/ni762","article-title":"Visualizing priming of virus-specific CD8+ T cells by infected dendritic cells in vivo","volume":"3","author":"Norbury","year":"2002","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.jviromet.2008.02.009_bib13","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1073\/pnas.85.5.1667","article-title":"Expression of the firefly luciferase gene in vaccinia virus: a highly sensitive gene marker to follow virus dissemination in tissues of infected animals","volume":"85","author":"Rodriguez","year":"1988","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.jviromet.2008.02.009_bib14","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.ijid.2005.12.001","article-title":"Vaccinia immune globulin: current policies, preparedness, and product safety and efficacy","volume":"10","author":"Wittek","year":"2005","journal-title":"Int. J. Infect. Dis."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093408000724?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093408000724?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,3]],"date-time":"2020-05-03T18:43:55Z","timestamp":1588531435000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093408000724"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,6]]},"references-count":14,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2008,6]]}},"alternative-id":["S0166093408000724"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2008.02.009","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2008,6]]}}}