{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T22:25:17Z","timestamp":1770503117527,"version":"3.49.0"},"reference-count":46,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2008,7]]},"DOI":"10.1016\/j.jviromet.2008.03.023","type":"journal-article","created":{"date-parts":[[2008,5,14]],"date-time":"2008-05-14T08:32:27Z","timestamp":1210753947000},"page":"47-54","source":"Crossref","is-referenced-by-count":33,"title":["Multiplex real-time PCR for the detection and quantification of latent and persistent viral genomes in cellular or plasma blood fractions"],"prefix":"10.1016","volume":"151","author":[{"given":"Lara Isobel","family":"Compston","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis","family":"Sarkobie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengyao","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Candotti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ohene","family":"Opare-Sem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Pierre","family":"Allain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2008.03.023_bib1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.jcv.2003.09.017","article-title":"Epidemiology of HHV-8 infection in HIV-positive patients with and without Kaposi sarcoma: diagnostic relevance of serology and PCR","volume":"30","author":"Albrecht","year":"2004","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib2","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1111\/j.1751-2824.2007.00090.x","article-title":"Differentiating transfusion-transmitted infection from reactivation of viruses","volume":"2","author":"Allain","year":"2007","journal-title":"ISBT Sci. Ser."},{"key":"10.1016\/j.jviromet.2008.03.023_bib3","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jviromet.2008.03.023_bib4","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jviromet.2005.01.020","article-title":"Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses","volume":"126","author":"Bellau-Pujol","year":"2005","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2008.03.023_bib5","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1128\/JCM.44.1.47-50.2006","article-title":"Positive correlation between Epstein\u2013Barr virus viral load and anti-viral capsid immunoglobulin G titers determined for Hodgkin's lymphoma patients and their relatives","volume":"44","author":"Besson","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib6","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1677\/jme.0.0250169","article-title":"Absolute quantification of mRNA using real-time reverse transcription chain reaction assays","volume":"2","author":"Bustin","year":"2000","journal-title":"J. Mol. Endocrinol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib7","series-title":"Advances in Transfusion Safety, vols. 4 and 127. Karger, International conference. Sydney, October 2005","first-page":"71","article-title":"The utility of multiplex NAT in blood screening","author":"Candotti","year":"2007"},{"key":"10.1016\/j.jviromet.2008.03.023_bib8","doi-asserted-by":"crossref","first-page":"12169","DOI":"10.1128\/JVI.78.22.12169-12178.2004","article-title":"Identification and characterization of persistent human erythrovirus infection in blood donor samples","volume":"78","author":"Candotti","year":"2004","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib9","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1111\/j.1365-2893.2006.00741.x","article-title":"Molecular and serological characterization of hepatitis B virus in deferred Ghanaian blood donors with and without elevated alanine aminotransferase","volume":"13","author":"Candotti","year":"2006","journal-title":"J. Viral Hepatitis"},{"key":"10.1016\/j.jviromet.2008.03.023_bib10","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.jviromet.2004.01.017","article-title":"Multiplex real-time quantitative RT-PCR assay for hepatitis B virus, hepatitis C virus, and human immunodeficiency virus type 1","volume":"118","author":"Candotti","year":"2004","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2008.03.023_bib11","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1182\/blood-2005-07-2746","article-title":"Pretransplantation CMV-specific T cells protect recipients of T-cell-depleted grafts against CMV-related complications","volume":"107","author":"Chalandon","year":"2006","journal-title":"Blood"},{"key":"10.1016\/j.jviromet.2008.03.023_bib12","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1182\/blood-2005-03-1039","article-title":"Donor KIR genotype has a major influence on the rate of cytomegalovirus reactivation following T-cell replete stem cell transplantation","volume":"107","author":"Cook","year":"2006","journal-title":"Blood"},{"key":"10.1016\/j.jviromet.2008.03.023_bib13","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1002\/jmv.2047","article-title":"Evidence for persistence of parvovirus B19 DNA in livers of adults","volume":"65","author":"Eis-Hubinger","year":"2001","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib14","doi-asserted-by":"crossref","first-page":"2856","DOI":"10.1128\/JCM.39.8.2856-2859.2001","article-title":"Quantitation of Varicella-Zoster virus DNA in patients with Ramsay Hunt syndrome and zoster sine herpete","volume":"39","author":"Furuta","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib15","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0928-0197(98)00064-6","article-title":"Prevalence of herpes simplex virus types 1 and 2, cytomegalovirus, and Varicella-Zoster virus infections in Eritrea","volume":"12","author":"Ghebrekidan","year":"1999","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib16","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.diagmicrobio.2006.09.012","article-title":"Relationship between human herpesvirus 8 loads and disease stage in classic Kaposi sarcoma patients","volume":"57","author":"Guttman-Yassky","year":"2007","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.jviromet.2008.03.023_bib17","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1128\/JCM.40.3.933-936.2002","article-title":"Prevalence of parvovirus B19 and parvovirus V9 DNA and antibodies in paired bone marrow and serum samples from healthy individuals","volume":"40","author":"Heegaard","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib18","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.ab.2006.10.016","article-title":"Real-time PCR: duplexing without optimisation","volume":"360","author":"Hein","year":"2007","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jviromet.2008.03.023_bib19","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jviromet.2005.05.030","article-title":"Validation of a real-time RT-PCR assay for sensitive and specific detection of classical swine fever","volume":"130","author":"Hoffmann","year":"2005","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2008.03.023_bib20","first-page":"23","article-title":"The PCR plateau phase\u2014towards an understanding of its limitations","volume":"1494","author":"Kainz","year":"2000","journal-title":"Biochem. Biophys. Acta"},{"key":"10.1016\/j.jviromet.2008.03.023_bib21","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1128\/JCM.37.1.132-136.1999","article-title":"Quantitative analysis of Epstein\u2013Barr virus load by using a real-time PCR assay","volume":"37","author":"Kimura","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib22","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S1471-4914(02)02355-9","article-title":"Quantification using real-time PCR technology: applications and limitations","volume":"8","author":"Klein","year":"2002","journal-title":"Trends Mol. Med."},{"key":"10.1016\/j.jviromet.2008.03.023_bib23","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1002\/(SICI)1096-9071(199909)59:1<32::AID-JMV6>3.0.CO;2-O","article-title":"GBV-C\/hepatitis G virus (HGV) RNA load in immunodeficient individuals and in immunocompetent individuals","volume":"59","author":"Lefrere","year":"1999","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib24","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1097\/01.aids.0000200536.79360.03","article-title":"GB virus C and HIV-1 RNA load in single virus and co-infected West African individuals","volume":"20","author":"Li","year":"2006","journal-title":"AIDS"},{"key":"10.1016\/j.jviromet.2008.03.023_bib25","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/S0006-291X(02)00478-3","article-title":"Validation of a quantitative method for real time PCR kinetics","volume":"294","author":"Liu","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jviromet.2008.03.023_bib26","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1093\/nar\/30.6.1292","article-title":"Real-time PCR in virology","volume":"30","author":"Mackay","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jviromet.2008.03.023_bib27","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1086\/499277","article-title":"High levels of Epstein\u2013Barr virus DNA in saliva and peripheral blood from Ugandan mother-child pairs","volume":"193","author":"Mbulaiteye","year":"2006","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jviromet.2008.03.023_bib28","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1086\/428910","article-title":"Exposure to holoendemic malaria results in elevated Epstein\u2013Barr virus loads in children","volume":"191","author":"Moorman","year":"2005","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jviromet.2008.03.023_bib29","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1111\/j.1537-2995.2004.04279.x","article-title":"Predonation screening of blood donors with rapid tests: implementation and efficacy of a novel approach to blood safety in resource-poor settings","volume":"45","author":"Owusu-Ofori","year":"2005","journal-title":"Transfusion"},{"key":"10.1016\/j.jviromet.2008.03.023_bib30","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1182\/blood.V100.2.677","article-title":"Persistence of HTLV-I in blood components after leukocyte depletion","volume":"100","author":"Pennington","year":"2002","journal-title":"Blood"},{"key":"10.1016\/j.jviromet.2008.03.023_bib31","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.ab.2005.06.026","article-title":"Four-color multiplex reverse transcription polymerase chain reaction\u2014overcoming its limitations","volume":"344","author":"Persson","year":"2005","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jviromet.2008.03.023_bib32","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S1386-6532(99)00078-5","article-title":"Detection of human herpesvirus 8 DNA and antibodies to latent nuclear and lytic-phase antigens in serial samples from aids patients with Karposi sarcoma","volume":"16","author":"Pierrotti","year":"2000","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib33","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1006\/mcpr.1999.0278","article-title":"Symmetric vs asymmetric PCR and molecular beacon probe in the detection of a target gene of adenovirus","volume":"14","author":"Podder","year":"2000","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.jviromet.2008.03.023_bib34","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1111\/j.1365-3083.2005.01589.x","article-title":"Circulating Epstein\u2013Barr viruses in children living in malaria-endemic areas","volume":"61","author":"Rasti","year":"2005","journal-title":"Scand. J. Immunol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib35","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1136\/mp.54.2.86","article-title":"Recovery efficiencies on nucleic acid extraction kits as measured by quantitative LightCycler PCR","volume":"54","author":"Read","year":"2001","journal-title":"Mol. Pathol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib36","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1073\/pnas.0305476101","article-title":"Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative real-time analysis","volume":"101","author":"Sanchez","year":"2004","journal-title":"PNAS"},{"key":"10.1016\/j.jviromet.2008.03.023_bib37","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1046\/j.1365-2613.2000.00166.x","article-title":"GB virus C\/hepatitis G virus (GBV-C\/HGV): still looking for a disease","volume":"81","author":"Sathar","year":"2000","journal-title":"Int. J. Exp. Pathol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib38","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1093\/qjmed\/90.8.497","article-title":"Post-transplantation lymphoproliferative disease","volume":"90","author":"Savage","year":"1997","journal-title":"QJM"},{"key":"10.1016\/j.jviromet.2008.03.023_bib39","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1101\/gr.5.5.488","article-title":"A simplified procedure for developing multiplex PCRs","volume":"5","author":"Shuber","year":"1995","journal-title":"Genome Res."},{"key":"10.1016\/j.jviromet.2008.03.023_bib40","doi-asserted-by":"crossref","first-page":"4806","DOI":"10.1128\/JVI.73.6.4806-4812.1999","article-title":"Quantitative analysis of latent human cytomegalovirus","volume":"73","author":"Slobedman","year":"1999","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2008.03.023_bib41","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S1473-3099(06)70419-4","article-title":"HIV and GB virus C coinfection","volume":"6","author":"Stapleton","year":"2006","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.jviromet.2008.03.023_bib42","doi-asserted-by":"crossref","first-page":"e122","DOI":"10.1093\/nar\/gng122","article-title":"Standardized determination of real-time PCR efficiency from a single reaction set-up","volume":"31","author":"Tichopad","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jviromet.2008.03.023_bib43","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1182\/blood-2003-03-0762","article-title":"Assessment of removal of human cytomegalovirus from blood components by leukocyte depletion filters using real-time quantitative PCR","volume":"103","author":"Visconti","year":"2004","journal-title":"Blood"},{"key":"10.1016\/j.jviromet.2008.03.023_bib44","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.mam.2005.12.001","article-title":"Detection and monitoring of virus infections by real-time PCR","volume":"27","author":"Watzinger","year":"2006","journal-title":"Mol. Aspects Med."},{"key":"10.1016\/j.jviromet.2008.03.023_bib45","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0166-0934(99)00154-8","article-title":"Sensitive and accurate quantitation of hepatitis B virus DNA using a kinetic fluorescence detection system (TaqMan PCR)","volume":"85","author":"Weinberger","year":"2000","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2008.03.023_bib46","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/j.trstmh.2005.08.009","article-title":"Persistent Epstein\u2013Barr viral reactivation in young African children with a history of severe Plasmodium falciparum malaria","volume":"100","author":"Yone","year":"2006","journal-title":"Trans. R. Soc. Trop. Med. Hyg."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093408001249?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093408001249?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T17:14:13Z","timestamp":1588698853000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093408001249"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,7]]}},"alternative-id":["S0166093408001249"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2008.03.023","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2008,7]]}}}