{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:40:13Z","timestamp":1791020413549,"version":"4.1.0"},"reference-count":57,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2010,5]]},"DOI":"10.1016\/j.jviromet.2010.01.018","type":"journal-article","created":{"date-parts":[[2010,2,5]],"date-time":"2010-02-05T09:18:35Z","timestamp":1265361515000},"page":"186-197","source":"Crossref","is-referenced-by-count":47,"title":["Development of an in vitro dual-chamber model of the female genital tract as a screening tool for epithelial toxicity"],"prefix":"10.1016","volume":"165","author":[{"given":"Youssef","family":"Gali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin K.","family":"Ari\u00ebn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marleen","family":"Praet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Van den Bergh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marleen","family":"Temmerman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Delezay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Vanham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2010.01.018_bib1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s004180050172","article-title":"High sensitivity detection of HPV-16 in SiHa and CaSki cells utilizing FISH enhanced by TSA","volume":"108","author":"Adler","year":"1997","journal-title":"Histochem. Cell Biol."},{"key":"10.1016\/j.jviromet.2010.01.018_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/QAD.0b013e328333acfb","article-title":"Caveats associated with the use of human cervical tissue for HIV and microbicide research","volume":"24","author":"Anderson","year":"2010","journal-title":"AIDS"},{"key":"10.1016\/j.jviromet.2010.01.018_bib3","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1128\/JVI.61.4.962-971.1987","article-title":"Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines","volume":"61","author":"Baker","year":"1987","journal-title":"J. Virol."},{"issue":"Pt 5","key":"10.1016\/j.jviromet.2010.01.018_bib4","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1242\/jcs.111.5.541","article-title":"Tight junctions","volume":"111","author":"Balda","year":"1998","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.jviromet.2010.01.018_bib5","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1083\/jcb.134.4.1031","article-title":"Functional dissociation of paracellular permeability and transepithelial electrical resistance and disruption of the apical-basolateral intramembrane diffusion barrier by expression of a mutant tight junction membrane protein","volume":"134","author":"Balda","year":"1996","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.jviromet.2010.01.018_bib6","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1128\/AAC.50.2.713-723.2006","article-title":"In vitro preclinical testing of nonoxynol-9 as potential anti-human immunodeficiency virus microbicide: a retrospective analysis of results from five laboratories","volume":"50","author":"Beer","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jviromet.2010.01.018_bib7","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/S0928-0987(03)00146-5","article-title":"Do cell culture conditions influence the carrier-mediated transport of peptides in Caco-2 cell monolayers?","volume":"19","author":"Behrens","year":"2003","journal-title":"Eur. J. Pharm. Sci."},{"key":"10.1016\/j.jviromet.2010.01.018_bib8","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0166-0934(98)00044-5","article-title":"Design and evaluation of an in-house HIV-1 (group M and O), SIVmnd and SIVcpz antigen capture assay","volume":"73","author":"Beirnaert","year":"1998","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2010.01.018_bib9","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/S0891-5520(05)70143-4","article-title":"Biologic approaches to the prevention of sexual transmission of human immunodeficiency virus","volume":"14","author":"Blocker","year":"2000","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"10.1016\/j.jviromet.2010.01.018_bib10","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1128\/JVI.01303-06","article-title":"Cell-free human immunodeficiency virus type 1 transcytosis through primary genital epithelial cells","volume":"81","author":"Bobardt","year":"2007","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2010.01.018_bib11","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1038\/nm0197-42","article-title":"Transcytosis of infectious human immunodeficiency virus across a tight human epithelial cell line barrier","volume":"3","author":"Bomsel","year":"1997","journal-title":"Nat. Med."},{"key":"10.1016\/j.jviromet.2010.01.018_bib12","doi-asserted-by":"crossref","first-page":"3652","DOI":"10.1073\/pnas.91.9.3652","article-title":"Monocyte chemoattractant protein 1 acts as a T-lymphocyte chemoattractant","volume":"91","author":"Carr","year":"1994","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.jviromet.2010.01.018_bib13","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1128\/JVI.76.1.32-40.2002","article-title":"Binding of LFA-1 (CD11a) to intercellular adhesion molecule 3 (ICAM-3; CD50) and ICAM-2 (CD102) triggers transmigration of human immunodeficiency virus type 1-infected monocytes through mucosal epithelial cells","volume":"76","author":"Carreno","year":"2002","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2010.01.018_bib14","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1097\/00002030-200303070-00001","article-title":"Recent observations on HIV type-1 infection in the genital tract of men and women","volume":"17","author":"Coombs","year":"2003","journal-title":"AIDS"},{"key":"10.1016\/j.jviromet.2010.01.018_bib15","doi-asserted-by":"crossref","first-page":"1770","DOI":"10.1128\/AAC.01129-06","article-title":"Preclinical testing of candidate topical microbicides for anti-human immunodeficiency virus type 1 activity and tissue toxicity in a human cervical explant culture","volume":"51","author":"Cummins","year":"2007","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jviromet.2010.01.018_bib16","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/S1473-3099(08)70254-8","article-title":"Vaginal microbicides and the prevention of HIV transmission","volume":"8","author":"Cutler","year":"2008","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.jviromet.2010.01.018_bib17","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1086\/319676","article-title":"Cervical and prostate primary epithelial cells are not productively infected but sequester human immunodeficiency virus type 1","volume":"183","author":"Dezzutti","year":"2001","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jviromet.2010.01.018_bib18","doi-asserted-by":"crossref","first-page":"3834","DOI":"10.1128\/AAC.48.10.3834-3844.2004","article-title":"In vitro comparison of topical microbicides for prevention of human immunodeficiency virus type 1 transmission","volume":"48","author":"Dezzutti","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jviromet.2010.01.018_bib19","doi-asserted-by":"crossref","first-page":"2231","DOI":"10.1097\/00002030-200111230-00003","article-title":"Vaginal transmission of HIV-1 in hu-SCID mice: a new model for the evaluation of vaginal microbicides","volume":"15","author":"Di Fabio","year":"2001","journal-title":"AIDS"},{"issue":"Suppl. 3","key":"10.1016\/j.jviromet.2010.01.018_bib20","first-page":"S43","article-title":"Female-controlled methods to prevent sexual transmission of HIV","volume":"10","author":"Elias","year":"1996","journal-title":"AIDS"},{"key":"10.1016\/j.jviromet.2010.01.018_bib21","doi-asserted-by":"crossref","first-page":"e1474","DOI":"10.1371\/journal.pone.0001474","article-title":"SAVVY vaginal gel (C31G) for prevention of HIV infection: a randomized controlled trial in Nigeria","volume":"3","author":"Feldblum","year":"2008","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jviromet.2010.01.018_bib22","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1095\/biolreprod.104.029603","article-title":"Interleukin (IL)-1, IL-6, and IL-8 predict mucosal toxicity of vaginal microbicidal contraceptives","volume":"71","author":"Fichorova","year":"2004","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.jviromet.2010.01.018_bib23","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1095\/biolreprod57.4.847","article-title":"Generation of papillomavirus-immortalized cell lines from normal human ectocervical, endocervical, and vaginal epithelium that maintain expression of tissue-specific differentiation proteins","volume":"57","author":"Fichorova","year":"1997","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.jviromet.2010.01.018_bib24","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1086\/322047","article-title":"The molecular basis of nonoxynol-9-induced vaginal inflammation and its possible relevance to human immunodeficiency virus type 1 transmission","volume":"184","author":"Fichorova","year":"2001","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jviromet.2010.01.018_bib25","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1128\/AAC.01156-08","article-title":"Inhibition of human immunodeficiency virus type 1 infection by the candidate microbicide dapivirine, a nonnucleoside reverse transcriptase inhibitor","volume":"53","author":"Fletcher","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jviromet.2010.01.018_bib26","doi-asserted-by":"crossref","first-page":"12559","DOI":"10.1073\/pnas.91.26.12559","article-title":"Infection of vaginal and colonic epithelial cells by the human immunodeficiency virus type 1 is neutralized by antibodies raised against conserved epitopes in the envelope glycoprotein gp120","volume":"91","author":"Furuta","year":"1994","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.jviromet.2010.01.018_bib27","doi-asserted-by":"crossref","first-page":"5577","DOI":"10.1128\/JVI.74.12.5577-5586.2000","article-title":"Parameters of human immunodeficiency virus infection of human cervical tissue and inhibition by vaginal virucides","volume":"74","author":"Greenhead","year":"2000","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2010.01.018_bib28","doi-asserted-by":"crossref","first-page":"4155","DOI":"10.1128\/IAI.73.7.4155-4160.2005","article-title":"Trichomonas vaginalis-induced epithelial monolayer disruption and human immunodeficiency virus type 1 (HIV-1) replication: implications for the sexual transmission of HIV-1","volume":"73","author":"Guenthner","year":"2005","journal-title":"Infect. Immun."},{"key":"10.1016\/j.jviromet.2010.01.018_bib29","doi-asserted-by":"crossref","first-page":"e3784","DOI":"10.1371\/journal.pone.0003784","article-title":"Effectiveness of cellulose sulfate vaginal gel for the prevention of HIV infection: results of a phase III trial in Nigeria","volume":"3","author":"Halpern","year":"2008","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jviromet.2010.01.018_bib30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/01.qai.0000159671.25950.74","article-title":"In vitro and in vivo: the story of nonoxynol 9","volume":"39","author":"Hillier","year":"2005","journal-title":"J. Acquir. Immune Defic. Syndr."},{"key":"10.1016\/j.jviromet.2010.01.018_bib31","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1128\/CDLI.7.3.515-518.2000","article-title":"Secretory Leukocyte Protease Inhibitor inhibits infection of monocytes and lymphocytes with human immunodeficiency virus type 1 but does not interfere with transcytosis of cell-associated virus across tight epithelial barriers","volume":"7","author":"Hocini","year":"2000","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.jviromet.2010.01.018_bib32","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1172\/JCI0213236","article-title":"Vaginal transmission of cell-associated HIV-1 in the mouse is blocked by a topical, membrane-modifying agent","volume":"109","author":"Khanna","year":"2002","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.jviromet.2010.01.018_bib33","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1111\/j.1749-6632.1987.tb25004.x","article-title":"Role of basement membranes in cell differentiation","volume":"513","author":"Kleinman","year":"1987","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.jviromet.2010.01.018_bib34","doi-asserted-by":"crossref","first-page":"2292","DOI":"10.1128\/AAC.46.7.2292-2298.2002","article-title":"Comparative in vitro sensitivities of human immune cell lines, vaginal and cervical epithelial cell lines, and primary cells to candidate microbicides nonoxynol 9, C31G, and sodium dodecyl sulfate","volume":"46","author":"Krebs","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jviromet.2010.01.018_bib35","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1016\/0002-9378(72)90861-7","article-title":"Establishment of a cell line of human endometrial adenocarcinoma in vitro","volume":"114","author":"Kuramoto","year":"1972","journal-title":"Am. J. Obstet. Gynecol."},{"key":"10.1016\/j.jviromet.2010.01.018_bib36","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1038\/nri1848","article-title":"Microbicides and other topical strategies to prevent vaginal transmission of HIV","volume":"6","author":"Lederman","year":"2006","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.jviromet.2010.01.018_bib37","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1038\/nature07831","article-title":"Glycerol monolaurate prevents mucosal SIV transmission","volume":"458","author":"Li","year":"2009","journal-title":"Nature"},{"key":"10.1016\/j.jviromet.2010.01.018_bib38","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1182\/blood.V90.3.1141","article-title":"Inhibition of human immunodeficiency virus type 1 infectivity by Secretory Leukocyte Protease Inhibitor occurs prior to viral reverse transcription","volume":"90","author":"McNeely","year":"1997","journal-title":"Blood"},{"key":"10.1016\/j.jviromet.2010.01.018_bib39","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1086\/600867","article-title":"Disruption of tight junctions by cellulose sulfate facilitates HIV infection: model of microbicide safety","volume":"200","author":"Mesquita","year":"2009","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jviromet.2010.01.018_bib40","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1089\/aid.2005.21.228","article-title":"IL-8 increases transmission of HIV type 1 in cervical explant tissue","volume":"21","author":"Narimatsu","year":"2005","journal-title":"AIDS Res. Hum. Retrovir."},{"key":"10.1016\/j.jviromet.2010.01.018_bib41","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.jri.2006.02.004","article-title":"Distribution of simian immunodeficiency virus target cells in vaginal tissues of normal rhesus macaques: implications for virus transmission","volume":"72","author":"Poonia","year":"2006","journal-title":"J. Reprod. Immunol."},{"key":"10.1016\/j.jviromet.2010.01.018_bib42","doi-asserted-by":"crossref","first-page":"229","DOI":"10.2174\/1566524033479870","article-title":"Dendritic cells as a conduit to improve HIV vaccines","volume":"3","author":"Pope","year":"2003","journal-title":"Curr. Mol. Med."},{"key":"10.1016\/j.jviromet.2010.01.018_bib43","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1095\/biolreprod.105.043133","article-title":"Immunological microenvironments in the human vagina and cervix: mediators of cellular immunity are concentrated in the cervical transformation zone","volume":"73","author":"Pudney","year":"2005","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.jviromet.2010.01.018_bib44","first-page":"493","article-title":"A simple method of estimating fifty percent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Hyg."},{"key":"10.1016\/j.jviromet.2010.01.018_bib45","doi-asserted-by":"crossref","first-page":"5564","DOI":"10.1128\/JVI.65.10.5564-5568.1991","article-title":"Characterization of a novel human papillomavirus DNA in the cervical carcinoma cell line ME180","volume":"65","author":"Reuter","year":"1991","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2010.01.018_bib46","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/1479-5876-5-28","article-title":"Differential in vitro inhibitory activity against HIV-1 of alpha-(1-3)- and alpha-(1-6)-d-mannose specific plant lectins: implication for microbicide development","volume":"5","author":"Saidi","year":"2007","journal-title":"J. Trans. Med."},{"key":"10.1016\/j.jviromet.2010.01.018_bib47","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/nrmicro729","article-title":"Inhibiting sexual transmission of HIV-1 infection","volume":"1","author":"Shattock","year":"2003","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.jviromet.2010.01.018_bib48","doi-asserted-by":"crossref","first-page":"3258","DOI":"10.1128\/JVI.01796-08","article-title":"Macrophages in vaginal but not intestinal mucosa are monocyte-like and permissive to human immunodeficiency virus type 1 infection","volume":"83","author":"Shen","year":"2009","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2010.01.018_bib49","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1016\/S0140-6736(08)61842-5","article-title":"Efficacy of Carraguard for prevention of HIV infection in women in South Africa: a randomised, double-blind, placebo-controlled trial","volume":"372","author":"Skoler-Karpoff","year":"2008","journal-title":"Lancet"},{"key":"10.1016\/j.jviromet.2010.01.018_bib50","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.4049\/jimmunol.172.4.2219","article-title":"HIV-infected Langerhans cells preferentially transmit virus to proliferating autologous CD4+ memory T cells located within Langerhans cell-T cell clusters","volume":"172","author":"Sugaya","year":"2004","journal-title":"J. Immunol."},{"key":"10.1016\/j.jviromet.2010.01.018_bib51","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1007\/BF01718823","article-title":"Cell-mediated infection of cervix derived epithelial cells with primary isolates of human immunodeficiency virus","volume":"141","author":"Tan","year":"1996","journal-title":"Arch. Virol."},{"key":"10.1016\/j.jviromet.2010.01.018_bib52","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.1128\/AAC.01627-07","article-title":"Human immunodeficiency virus type 1 (HIV-1) integration: a potential target for microbicides to prevent cell-free or cell-associated HIV-1 infection","volume":"52","author":"Terrazas-Aranda","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jviromet.2010.01.018_bib53","unstructured":"UNAIDS, 2008. Report on the global AIDS epidemic 2008. http:\/\/www.unaids.org."},{"key":"10.1016\/j.jviromet.2010.01.018_bib54","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1016\/S0140-6736(02)11079-8","article-title":"Effectiveness of COL-1492, a nonoxynol-9 vaginal gel, on HIV-1 transmission in female sex workers: a randomised controlled trial","volume":"360","author":"Van Damme","year":"2002","journal-title":"The Lancet"},{"key":"10.1016\/j.jviromet.2010.01.018_bib55","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.antiviral.2006.10.010","article-title":"A dual chamber model of female cervical mucosa for the study of HIV transmission and for the evaluation of candidate HIV microbicides","volume":"74","author":"Van Herrewege","year":"2007","journal-title":"Antivir. Res."},{"key":"10.1016\/j.jviromet.2010.01.018_bib56","doi-asserted-by":"crossref","first-page":"3319","DOI":"10.1016\/S0142-9612(02)00019-4","article-title":"How to optimize seeding and culturing of human osteoblast-like cells on various biomaterials","volume":"23","author":"Wiedmann-Al-Ahmad","year":"2002","journal-title":"Biomaterials"},{"key":"10.1016\/j.jviromet.2010.01.018_bib57","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1002\/jlb.60.3.365","article-title":"Human recombinant monocyte chemotactic protein and other C\u2013C chemokines bind and induce directional migration of dendritic cells in vitro","volume":"60","author":"Xu","year":"1996","journal-title":"J. Leukoc. Biol."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093410000194?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093410000194?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T09:28:49Z","timestamp":1634894929000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093410000194"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5]]},"references-count":57,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,5]]}},"alternative-id":["S0166093410000194"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2010.01.018","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2010,5]]}}}