{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T04:10:43Z","timestamp":1785816643630,"version":"3.56.0"},"reference-count":21,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Key Science and Technology Project","award":["05YFSZSF02500"],"award-info":[{"award-number":["05YFSZSF02500"]}]},{"name":"Applied Basic Research Foundation","award":["06YFJMJC13100"],"award-info":[{"award-number":["06YFJMJC13100"]}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program","doi-asserted-by":"crossref","award":["2007CB512800"],"award-info":[{"award-number":["2007CB512800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2010,10]]},"DOI":"10.1016\/j.jviromet.2010.07.031","type":"journal-article","created":{"date-parts":[[2010,8,6]],"date-time":"2010-08-06T09:00:33Z","timestamp":1281085233000},"page":"181-187","source":"Crossref","is-referenced-by-count":26,"title":["Enhanced specificity of real-time PCR for measurement of hepatitis B virus cccDNA using restriction endonuclease and plasmid-safe ATP-dependent DNase and selective primers"],"prefix":"10.1016","volume":"169","author":[{"given":"Ying-Tang","family":"Gao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Jun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng-mei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Jing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2010.07.031_bib0005","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1111\/j.1365-2893.2006.00775.x","article-title":"Quantitative analysis of HBV cccDNA from clinical specimens: correlation with clinical and virological response during antiviral therapy","volume":"14","author":"Bourne","year":"2007","journal-title":"J. Viral Hepat."},{"key":"10.1016\/j.jviromet.2010.07.031_bib0010","first-page":"41","article-title":"Quantification of HBV covalently closed circular DNA from liver tissue by real-time PCR","volume":"95","author":"Bowden","year":"2004","journal-title":"Methods Mol. Med."},{"key":"10.1016\/j.jviromet.2010.07.031_bib0015","doi-asserted-by":"crossref","first-page":"82","DOI":"10.3748\/wjg.v10.i1.82","article-title":"HBV cccDNA in patient's sera as an indicator for HBV reactivation and an early signal of liver damage","volume":"10","author":"Chen","year":"2004","journal-title":"World J. Gastroenterol."},{"key":"10.1016\/j.jviromet.2010.07.031_bib0020","doi-asserted-by":"crossref","first-page":"6164","DOI":"10.1128\/JVI.02721-06","article-title":"Formation of hepatitis B virus covalently closed circular DNA: removal of genome-linked protein","volume":"81","author":"Gao","year":"2007","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2010.07.031_bib0025","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1016\/S0006-291X(02)00813-6","article-title":"A new and sensitive method for the quantification of HBV cccDNA by real-time PCR","volume":"295","author":"He","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jviromet.2010.07.031_bib0030","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0166-0934(03)00190-3","article-title":"A quantitative method to detect HBV cccDNA by chimeric primer and real-time polymerase chain reaction","volume":"112","author":"Jun-Bin","year":"2003","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2010.07.031_bib0035","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1053\/jhep.1996.v23.pm0008617418","article-title":"Hepatitis B virus nucleic acids associated with human peripheral blood mononuclear cells do not originate from replicating virus","volume":"23","author":"Kock","year":"1996","journal-title":"Hepatology"},{"key":"10.1016\/j.jviromet.2010.07.031_bib0040","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1002\/hep.21299","article-title":"Intrahepatic levels and replicative activity of covalently closed circular hepatitis B virus DNA in chronically infected patients","volume":"44","author":"Laras","year":"2006","journal-title":"Hepatology"},{"key":"10.1016\/j.jviromet.2010.07.031_bib0045","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1046\/j.1365-2893.2003.00487.x","article-title":"Hepatitis B virus epidemiology, disease burden, treatment, and current and emerging prevention and control measures","volume":"11","author":"Lavanchy","year":"2004","journal-title":"J. Viral Hepat."},{"key":"10.1016\/j.jviromet.2010.07.031_bib0050","first-page":"311A","article-title":"Selective PCR for hepatitis B virus ccc DNA: validation of real-time assays","volume":"34","author":"Littlejohn","year":"2001","journal-title":"Hepatology"},{"key":"10.1016\/j.jviromet.2010.07.031_bib0055","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.jviromet.2010.07.031_bib0060","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1053\/jhep.2001.29401","article-title":"Chronic hepatitis B","volume":"34","author":"Lok","year":"2001","journal-title":"Hepatology"},{"key":"10.1016\/j.jviromet.2010.07.031_bib0065","doi-asserted-by":"crossref","first-page":"S303","DOI":"10.1053\/j.gastro.2004.09.045","article-title":"Prevention of hepatitis B virus related hepatocellular carcinoma","volume":"127","author":"Lok","year":"2004","journal-title":"Gastroenterology"},{"key":"10.1016\/j.jviromet.2010.07.031_bib0070","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.jviromet.2006.07.019","article-title":"Real-time PCR quantitation of hepatitis B virus total DNA and covalently closed circular DNA in peripheral blood mononuclear cells from hepatitis B virus-infected patients","volume":"138","author":"Mazet-Wagner","year":"2006","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2010.07.031_bib0075","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.jviromet.2004.02.006","article-title":"Quantitation of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) in the liver of HBV-infected patients by LightCycler\u2122 real-time PCR","volume":"118","author":"Singh","year":"2004","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2010.07.031_bib0080","doi-asserted-by":"crossref","first-page":"1890","DOI":"10.1053\/j.gastro.2005.03.009","article-title":"Intrahepatic hepatitis B virus covalently closed circular DNA can be a predictor of sustained response to therapy","volume":"128","author":"Sung","year":"2005","journal-title":"Gastroenterology"},{"key":"10.1016\/j.jviromet.2010.07.031_bib0085","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1002\/jmv.21477","article-title":"Validation of a sensitive and specific real-time PCR for detection and quantitation of hepatitis B virus covalently closed circular DNA in plasma of chronic hepatitis B patients","volume":"81","author":"Takkenberg","year":"2009","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jviromet.2010.07.031_bib0090","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1053\/j.gastro.2004.03.018","article-title":"Persistence of cccDNA during the natural history of chronic hepatitis B and decline during adefovir dipivoxil therapy","volume":"126","author":"Werle-Lapostolle","year":"2004","journal-title":"Gastroenterology"},{"key":"10.1016\/j.jviromet.2010.07.031_bib0095","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1002\/hep.20353","article-title":"Quantitation of covalently closed circular hepatitis virus DNA in chronic hepatitis B patients","volume":"40","author":"Wong","year":"2004","journal-title":"Hepatology"},{"key":"10.1016\/j.jviromet.2010.07.031_bib0100","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/j.jhep.2006.05.014","article-title":"Quantification of hepatitis B virus covalently closed circular DNA in patients with hepatocellular carcinoma","volume":"45","author":"Wong","year":"2006","journal-title":"J. Hepatol."},{"key":"10.1016\/j.jviromet.2010.07.031_bib0105","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.jhep.2004.12.015","article-title":"New insight on hepatitis B virus persistence from the study of intrahepatic viral cccDNA","volume":"42","author":"Zoulim","year":"2005","journal-title":"J. Hepatol."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093410002739?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093410002739?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T20:02:16Z","timestamp":1588795336000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093410002739"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,10]]}},"alternative-id":["S0166093410002739"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2010.07.031","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2010,10]]}}}