{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T02:53:59Z","timestamp":1780714439451,"version":"3.54.1"},"reference-count":27,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2010,12]]},"DOI":"10.1016\/j.jviromet.2010.08.021","type":"journal-article","created":{"date-parts":[[2010,9,3]],"date-time":"2010-09-03T05:24:51Z","timestamp":1283491491000},"page":"160-164","source":"Crossref","is-referenced-by-count":7,"title":["Quantitation of HTLV-I proviral load by a real-time PCR assay using SYBR Green: Comparison of two methods for DNA isolation"],"prefix":"10.1016","volume":"170","author":[{"given":"Natalia Andrea","family":"Altamirano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Rocco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Aulicino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa","family":"Sen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Mangano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.jviromet.2010.08.021_bib0005","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1128\/JCM.02006-08","article-title":"One-step, multiplex, real-time PCR assay with molecular beacon probes for simultaneous detection, differentiation, and quantification of human T-cell leukemia virus types 1, 2, and 3","volume":"47","author":"Besson","year":"2009","journal-title":"J. Clin. Microbiol."},{"issue":"4","key":"10.1016\/j.jviromet.2010.08.021_bib0010","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1097\/00126334-200304010-00015","article-title":"A cluster of human T-cell lymphotropic virus type I-associated myelopathy\/tropical spastic paraparesis in Jujuy, Argentina","volume":"32","author":"Biglione","year":"2003","journal-title":"J. Acquir. Immune Defic. Syndr."},{"issue":"12","key":"10.1016\/j.jviromet.2010.08.021_bib0015","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1097\/00126334-199212000-00014","article-title":"Prevalence of HTLV-I\/II among blood bank donors in Argentina","volume":"5","author":"Bouzas","year":"1992","journal-title":"J. Acquir. Immune Defic. Syndr."},{"issue":"11","key":"10.1016\/j.jviromet.2010.08.021_bib0020","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1089\/aid.1994.10.1567","article-title":"HTLV type I and HTLV type II infection among Indians and natives from Argentina","volume":"10","author":"Bouzas","year":"1994","journal-title":"AIDS Res. Hum. Retroviruses"},{"issue":"1","key":"10.1016\/j.jviromet.2010.08.021_bib0025","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1590\/S1020-49892006000100007","article-title":"HTLV in the Americas: challenges and perspectives","volume":"19","author":"Carneiro-Proietti","year":"2006","journal-title":"Rev. Panam. Salud P\u00fablica"},{"issue":"3\u20134","key":"10.1016\/j.jviromet.2010.08.021_bib0030","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.mcp.2005.12.003","article-title":"A comparison of five methods for extracting DNA from paucicellular clinical samples","volume":"20","author":"Cler","year":"2006","journal-title":"Mol. Cell. Probes"},{"issue":"1\u20132","key":"10.1016\/j.jviromet.2010.08.021_bib0035","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0166-0934(01)00445-1","article-title":"Quantitation of HTLV-I proviral load by a TaqMan real-time PCR assay","volume":"102","author":"Deh\u00e9e","year":"2002","journal-title":"J. Virol. Methods"},{"issue":"3","key":"10.1016\/j.jviromet.2010.08.021_bib0040","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1097\/00126334-200403010-00012","article-title":"Seroepidemiology of HTLV-I\/II in Argentina: an overview","volume":"35","author":"Gastaldello","year":"2004","journal-title":"J. Acquir. Immune Defic. Syndr."},{"issue":"8452","key":"10.1016\/j.jviromet.2010.08.021_bib0045","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/S0140-6736(85)92734-5","article-title":"Antibodies to human T-lymphotropic virus type-I in patients with tropical spastic paraparesis","volume":"2","author":"Gessain","year":"1985","journal-title":"Lancet"},{"key":"10.1016\/j.jviromet.2010.08.021_bib0050","doi-asserted-by":"crossref","DOI":"10.1182\/blood-2009-12-257410","article-title":"Human T-cell leukemia virus type I (HTLV-1) proviral load and disease progression in asymptomatic HTLV-1 carriers: a nationwide prospective study in Japan","author":"Iwanaga","year":"2010","journal-title":"Blood"},{"issue":"2","key":"10.1016\/j.jviromet.2010.08.021_bib0055","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1046\/j.1365-2257.2003.00503.x","article-title":"Significance of HTLV-1 proviral load quantification by real-time PCR as a surrogate marker for HTLV-1-infected cell count","volume":"25","author":"Kamihira","year":"2003","journal-title":"Clin. Lab. Haematol."},{"issue":"6","key":"10.1016\/j.jviromet.2010.08.021_bib0060","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1002\/j.1460-2075.1984.tb01974.x","article-title":"Genomic structure of HTLV (human T-cell leukemia virus): detection of defective genome and its amplification in MT2 cells","volume":"3","author":"Kobayashi","year":"1984","journal-title":"EMBO J."},{"issue":"4","key":"10.1016\/j.jviromet.2010.08.021_bib0065","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.jcv.2004.05.016","article-title":"Quantitation of HTLV-I and II proviral load using real-time quantitative PCR with SYBR Green chemistry","volume":"31","author":"Lee","year":"2004","journal-title":"J. Clin. Virol."},{"issue":"2","key":"10.1016\/j.jviromet.2010.08.021_bib0070","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1002\/jmv.20172","article-title":"Human T lymphotropic virus types I and II proviral sequences in Argentinian blood donors with indeterminate Western blot patterns","volume":"74","author":"Mangano","year":"2004","journal-title":"J. Med. Virol."},{"issue":"7","key":"10.1016\/j.jviromet.2010.08.021_bib0075","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1089\/088922200308891","article-title":"Real-time polymerase chain reaction assay for cell-associated HTLV type I DNA viral load","volume":"16","author":"Miley","year":"2000","journal-title":"AIDS Res. Hum. Retroviruses"},{"issue":"11","key":"10.1016\/j.jviromet.2010.08.021_bib0080","doi-asserted-by":"crossref","first-page":"3682","DOI":"10.1128\/JCM.00781-09","article-title":"Development and validation of a multiplex real-time PCR assay for simultaneous genotyping and human T-lymphotropic virus type 1, 2, and 3 proviral load determination","volume":"47","author":"Moens","year":"2009","journal-title":"J. Clin. Microbiol."},{"issue":"1\u20132","key":"10.1016\/j.jviromet.2010.08.021_bib0085","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jns.2005.05.010","article-title":"HTLV-1 proviral load in peripheral blood mononuclear cells quantified in 100 HAM\/TSP patients: a marker of disease progression","volume":"237","author":"Olindo","year":"2005","journal-title":"J. Neurol. Sci."},{"issue":"12","key":"10.1016\/j.jviromet.2010.08.021_bib0090","doi-asserted-by":"crossref","first-page":"7415","DOI":"10.1073\/pnas.77.12.7415","article-title":"Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma","volume":"77","author":"Poiesz","year":"1980","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"12","key":"10.1016\/j.jviromet.2010.08.021_bib0095","doi-asserted-by":"crossref","first-page":"2006","DOI":"10.1038\/sj.leu.2401246","article-title":"Real-time quantitative PCR for the detection of minimal residual disease in acute lymphoblastic leukemia using junctional region specific TaqMan probes","volume":"12","author":"Pongers Willemse","year":"1998","journal-title":"Leukemia"},{"issue":"8","key":"10.1016\/j.jviromet.2010.08.021_bib0100","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1590\/S0100-879X2009005000008","article-title":"High HTLV-1 proviral load, a marker for HTLV-1 associated myelopathy\/tropical spastic paraparesis, is also detected in patients with infective dermatitis associated with HTLV-1","volume":"42","author":"Primo","year":"2009","journal-title":"Braz. J. Med. Biol. Res."},{"issue":"39","key":"10.1016\/j.jviromet.2010.08.021_bib0105","doi-asserted-by":"crossref","first-page":"6058","DOI":"10.1038\/sj.onc.1208968","article-title":"Global epidemiology of HTLV-I infection and associated diseases","volume":"24","author":"Proietti","year":"2005","journal-title":"Oncogene"},{"issue":"4","key":"10.1016\/j.jviromet.2010.08.021_bib0110","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1046\/j.1423-0410.2002.00240.x","article-title":"Profile of human T-cell lymphocytotrophic virus-I\/II infections in an Argentinean blood bank population","volume":"83","author":"Remesar","year":"2002","journal-title":"Vox Sang."},{"key":"10.1016\/j.jviromet.2010.08.021_bib0115","series-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"10.1016\/j.jviromet.2010.08.021_bib0120","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/1743-422X-7-81","article-title":"High human T cell leukemia virus type-1(HTLV-1) provirus load in patients with HTLV-1 carriers complicated with HTLV-1-unrelated disorders","volume":"7","author":"Sasaki","year":"2010","journal-title":"Virol. J."},{"issue":"5","key":"10.1016\/j.jviromet.2010.08.021_bib0125","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1086\/510679","article-title":"Human T lymphotropic virus type I (HTLV-I) proviral load in asymptomatic carriers, HTLV-1-associated myelopathy\/tropical spastic paraparesis, and other neurological abnormalities associated with HTLV-I infection","volume":"44","author":"Silva","year":"2007","journal-title":"Clin. Infect. Dis."},{"issue":"1","key":"10.1016\/j.jviromet.2010.08.021_bib0130","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/0166-0934(94)00093-V","article-title":"Typing human T-cell lymphotropic virus (HTLV-I and HTLV-II) by nested polymerase chain reaction: application to clinical specimens","volume":"51","author":"Vallejo","year":"1995","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2010.08.021_bib0135","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/1471-2334-6-41","article-title":"Human T-lymphotropic virus type 1 (HTLV-1) prevalence and quantitative detection of DNA proviral load in individuals with indeterminate\/positive serological results","volume":"6","author":"Vitone","year":"2006","journal-title":"BMC Infect. Dis."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093410003137?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093410003137?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,6,3]],"date-time":"2019-06-03T06:28:40Z","timestamp":1559543320000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093410003137"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12]]},"references-count":27,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["S0166093410003137"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2010.08.021","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2010,12]]}}}