{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T18:54:31Z","timestamp":1715972071977},"reference-count":20,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T00:00:00Z","timestamp":1306886400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Madrid (Spain)","award":["PI3040363","2009SGR1159","EC0790642"],"award-info":[{"award-number":["PI3040363","2009SGR1159","EC0790642"]}]},{"name":"Spanish Network for AIDS Research","award":["ISCIII-RETIC RD06\/006"],"award-info":[{"award-number":["ISCIII-RETIC RD06\/006"]}]},{"name":"Institut d\u2019Investigacions Biom\u00e8diques August Pi i Sunyer (IDIBAPS)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2011,6]]},"DOI":"10.1016\/j.jviromet.2011.03.026","type":"journal-article","created":{"date-parts":[[2011,4,8]],"date-time":"2011-04-08T15:05:07Z","timestamp":1302275107000},"page":"69-76","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of the Roche COBAS\u00ae TaqMan\u00ae HIV-1 test for quantifying HIV-1 RNA in infected cells and lymphoid tissue"],"prefix":"10.1016","volume":"174","author":[{"given":"Cristina","family":"Gil","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Teresa","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felipe","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 M.","family":"Mir\u00f3","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Ag\u00fcero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc\u00eda","family":"Al\u00f3s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Zamora","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alicia","family":"Cap\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josep","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom\u00e1s","family":"Pumarola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 M.","family":"Gatell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2011.03.026_bib0005","doi-asserted-by":"crossref","first-page":"10958","DOI":"10.1073\/pnas.96.20.10958","article-title":"Latent reservoirs of HIV: obstacles to the eradication of virus","volume":"96","author":"Chun","year":"1999","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0010","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1073\/pnas.0437640100","article-title":"Gene expression and viral production in latently infected, resting CD4+ T cells in viremic versus aviremic HIV-infected individuals","volume":"100","author":"Chun","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0015","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1089\/088922201300119671","article-title":"Residual human immunodeficiency virus type 1 infection in lymphoid tissue during highly active antiretroviral therapy: quantitation and virus characterization","volume":"17","author":"Dyrhol-Riise","year":"2001","journal-title":"AIDS Res. Hum. Retroviruses"},{"key":"10.1016\/j.jviromet.2011.03.026_bib0020","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1128\/JCM.37.5.1260-1264.1999","article-title":"Highly sensitive methods for quantitation of human immunodeficiency virus type 1 RNA from plasma, cells, and tissues","volume":"37","author":"Fischer","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0030","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1084\/jem.164.1.280","article-title":"Biological and biochemical characterization of a cloned Leu-3-cell surviving infection with the acquired immune deficiency syndrome retrovirus","volume":"164","author":"Folks","year":"1986","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0035","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1086\/500213","article-title":"CD4 cell count and HIV DNA level are independent predictors of disease progression after primary HIV type 1 infection in untreated patients","volume":"42","author":"Goujard","year":"2006","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0040","doi-asserted-by":"crossref","first-page":"8236","DOI":"10.1128\/JVI.00120-06","article-title":"Viral suppression and immune restoration in the gastrointestinal mucosa of human immunodeficiency virus type 1-infected patients initiating therapy during primary or chronic infection","volume":"80","author":"Guadalupe","year":"2006","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0045","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1038\/nrmicro1580","article-title":"Experimental approaches to the study of HIV-1 latency","volume":"5","author":"Han","year":"2007","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0050","doi-asserted-by":"crossref","first-page":"1973","DOI":"10.1016\/S0002-9440(10)65070-5","article-title":"Treatment-induced decline of human immunodeficiency virus-1 p24 and HIV-1 RNA in lymphoid tissue of patients with early human immunodeficiency virus-1 infection","volume":"156","author":"Kuster","year":"2000","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0055","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1097\/00002030-200108170-00004","article-title":"Lymphoid tissue viral burden and duration of viral suppression in plasma","volume":"15","author":"Mart\u00ednez","year":"2001","journal-title":"AIDS"},{"key":"10.1016\/j.jviromet.2011.03.026_bib0060","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1177\/135965350300800612","article-title":"Predictors of tonsillar tissue HIV-1 viral burden at baseline and after 1 year of antiretroviral therapy","volume":"8","author":"Navarrete","year":"2003","journal-title":"Antivir. Ther."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0065","first-page":"178","article-title":"Sensitive procedure for the amplification of HIV-1 RNA using a combined reverse-transcription and amplification reaction","volume":"19","author":"Nijhuis","year":"1995","journal-title":"Biotechniques"},{"key":"10.1016\/j.jviromet.2011.03.026_bib0070","doi-asserted-by":"crossref","first-page":"14441","DOI":"10.1073\/pnas.95.24.14441","article-title":"Stochastic processes strongly influence HIV-1 evolution during suboptimal protease-inhibitor therapy","volume":"95","author":"Nijhuis","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0075","doi-asserted-by":"crossref","first-page":"2206","DOI":"10.1128\/JCM.00055-08","article-title":"Highly sensitive methods based on seminested real-time reverse transcription-PCR for quantitation of human immunodeficiency virus type 1 unspliced and multiply spliced RNA and proviral DNA","volume":"46","author":"Pasternak","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0080","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1126\/science.1165706","article-title":"The challenge of finding a cure for HIV infection","volume":"323","author":"Richman","year":"2009","journal-title":"Science"},{"key":"10.1016\/j.jviromet.2011.03.026_bib0085","doi-asserted-by":"crossref","first-page":"2209","DOI":"10.1128\/JCM.01761-08","volume":"47","author":"Scott","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0090","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.jcv.2010.06.004","article-title":"Improved HIV-1 RNA quantitation by COBAS AmpliPrep\/COBAS TaqMan HIV-1 Test, v2.0 using a novel dual-target approach","volume":"49","author":"Sizmann","year":"2010","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0095","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1086\/345870","article-title":"Prognostic value of baseline human immunodeficiency virus type 1 DNA measurement for disease progression in patients receiving nucleoside therapy","volume":"187","author":"Tierney","year":"2003","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0100","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jcv.2009.06.012","article-title":"Evaluation of the new VERSANT HIV-1 RNA 1.0 Assay (kPCR) for quantitative detection of human immunodeficiency virus type 1 RNA","volume":"46","author":"Troppan","year":"2009","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jviromet.2011.03.026_bib0105","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1086\/324002","article-title":"Human immunodeficiency virus type 1 RNA in peripheral blood mononuclear cells of patients receiving prolonged highly active antiretroviral therapy","volume":"184","author":"Zhang","year":"2001","journal-title":"J. Infect. Dis."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093411001170?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093411001170?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,6,6]],"date-time":"2023-06-06T05:59:47Z","timestamp":1686031187000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093411001170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,6]]},"references-count":20,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2011,6]]}},"alternative-id":["S0166093411001170"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2011.03.026","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2011,6]]}}}