{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:46:38Z","timestamp":1791477998065,"version":"4.3.4"},"reference-count":24,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2012,1]]},"DOI":"10.1016\/j.jviromet.2011.10.011","type":"journal-article","created":{"date-parts":[[2011,10,24]],"date-time":"2011-10-24T10:22:30Z","timestamp":1319451750000},"page":"135-141","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":32,"title":["Thermal treatment of the bacteriophage lysate of Klebsiella pneumoniae B5055 as a step for the purification of capsular depolymerase enzyme"],"prefix":"10.1016","volume":"179","author":[{"given":"Tesfaye","family":"Kassa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay","family":"Chhibber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2011.10.011_bib0005","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/0042-6822(56)90054-X","article-title":"An enzyme produced by a phage host cell system. II. The properties of the polysaccharide depolymerase","volume":"2","author":"Adams","year":"1956","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2011.10.011_bib0010","series-title":"Bacteriophages","author":"Adams","year":"1959"},{"key":"10.1016\/j.jviromet.2011.10.011_bib0015","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/j.1432-1033.1990.tb15491.x","article-title":"Bacteriophage-associated glycan hydrolases specific for Escherichia coli capsular serotype K12","volume":"185","author":"Altmann","year":"1990","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0020","doi-asserted-by":"crossref","first-page":"261","DOI":"10.2174\/138920108785161604","article-title":"The use of phages for the removal of infectious biofilms","volume":"9","author":"Azeredo","year":"2008","journal-title":"Curr. Pharm. Biotechnol."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0025","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/0042-6822(73)90293-6","article-title":"A bacteriophage-induced depolymerase active on Klebsiella K11 capsular polysaccharide","volume":"56","author":"Bessler","year":"1973","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2011.10.011_bib0030","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1128\/jvi.15.4.976-984.1975","article-title":"Escherichia coli capsule bacteriophages. IV. Free capsule depolymerase 29","volume":"15","author":"Bessler","year":"1975","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0035","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1021\/ac60111a017","article-title":"Colorimetric method for determination of sugars and related substances","volume":"28","author":"Dubios","year":"1956","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0040","doi-asserted-by":"crossref","first-page":"2746","DOI":"10.1128\/AEM.67.6.2746-2753.2001","article-title":"Reduction in exopolysaccharide viscosity as an aid to bacteriophage penetration through Pseudomonas aeruginosa biofilms","volume":"67","author":"Hanlon","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0045","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1046\/j.1365-2672.1998.853541.x","article-title":"Bacteriophage and associated polysaccharide depolymerases \u2013 novel tools for study of bacterial biofilms","volume":"85","author":"Hughes","year":"1998","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0050","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1094\/PHYTO.2000.90.11.1263","article-title":"Characterization of a viral EPS-depolymerase, a potential tool for control of fire blight","volume":"90","author":"Kim","year":"2000","journal-title":"Phytopathology"},{"key":"10.1016\/j.jviromet.2011.10.011_bib0055","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1099\/0022-1317-29-3-267","article-title":"Disruption of Vi bacteriophage III and localization of its deacetylase activity","volume":"29","author":"Kwiatkowski","year":"1975","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0060","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0065","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with Folin-phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0070","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1128\/AAC.48.5.1503-1508.2004","article-title":"Prevention and cure of systemic Escherichia coli K1 infection by modification of the bacterial phenotype","volume":"48","author":"Mushtaq","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0075","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1128\/jb.130.1.366-374.1977","article-title":"Klebsiella serotype 25 capsular polysaccharide: primary structure and depolymerization by a bacteriophage-borne glycanase","volume":"130","author":"Niemann","year":"1977","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0080","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1128\/jvi.15.4.964-975.1975","article-title":"Escherichia coli capsule bacteriophages. III. Fragments of bacteriophage 29","volume":"15","author":"Rieger","year":"1975","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0085","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1042\/bj0610586","article-title":"The determination of glucosamine and galactosamine","volume":"61","author":"Rondle","year":"1955","journal-title":"Biochem. J."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0090","series-title":"Molecular Cloning: A Laboratory Manual","article-title":"Bacteriophage (growth, purification and DNA extraction; SDS-polyacrylamide gel electrophoresis of proteins","author":"Sambrook","year":"1989"},{"key":"10.1016\/j.jviromet.2011.10.011_bib0095","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1128\/AAC.00741-07","article-title":"Treatment of experimental anthrax with recombinant capsule depolymerase","volume":"52","author":"Scorpio","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0100","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/0042-6822(71)90138-3","article-title":"Isolation of spike formed particles from bacteriophage lysates","volume":"45","author":"Strim","year":"1971","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2011.10.011_bib0105","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1099\/00221287-39-3-373","article-title":"Depolymerases for bacterial exopolysaccharides obtained from phage-infected bacteria","volume":"39","author":"Sutherland","year":"1965","journal-title":"J. Gen. Microbiol."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0110","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1080\/08927014.2010.511196","article-title":"Structural changes induced by a lytic bacteriophage make ciprofloxacin effective against older biofilm of Klebsiella pneumoniae","volume":"26","author":"Verma","year":"2010","journal-title":"Biofouling"},{"key":"10.1016\/j.jviromet.2011.10.011_bib0115","doi-asserted-by":"crossref","first-page":"986","DOI":"10.3201\/eid1307.070187","article-title":"Virulence characteristics of Klebsiella and clinical manifestations of Klebsiella pneumoniae bloodstream infections","volume":"13","author":"Yu","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.jviromet.2011.10.011_bib0120","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1099\/0022-1317-49-11-1003","article-title":"Role of bacterial capsule in local and systemic inflammatory responses of mice during pulmonary infection with Klebsiella pneumoniae","volume":"49","author":"Yoshida","year":"2000","journal-title":"J. Med. Microbiol."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093411004277?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093411004277?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T10:49:01Z","timestamp":1759056541000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093411004277"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["S0166093411004277"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2011.10.011","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2012,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Thermal treatment of the bacteriophage lysate of Klebsiella pneumoniae B5055 as a step for the purification of capsular depolymerase enzyme","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2011.10.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2011 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}