{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:15:52Z","timestamp":1791101752318,"version":"4.1.0"},"reference-count":36,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"European Commission Framework Program","award":["KBBE 213178"],"award-info":[{"award-number":["KBBE 213178"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2013,2]]},"DOI":"10.1016\/j.jviromet.2012.10.017","type":"journal-article","created":{"date-parts":[[2012,11,14]],"date-time":"2012-11-14T21:30:53Z","timestamp":1352928653000},"page":"327-332","source":"Crossref","is-referenced-by-count":58,"title":["Replication of hepatitis E virus in three-dimensional cell culture"],"prefix":"10.1016","volume":"187","author":[{"given":"A.","family":"Berto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.H.M.","family":"Van der Poel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Hakze-van der Honing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Martelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.M.","family":"La Ragione","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Inglese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Collins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Grierson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Johne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Reetz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Dastjerdi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Banks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2012.10.017_bib0005","doi-asserted-by":"crossref","first-page":"842","DOI":"10.3855\/jidc.612","article-title":"Frequency of HEV contamination in sewerage waters in Pakistan","volume":"4","author":"Ahmad","year":"2010","journal-title":"J. Infect. Dev. Countries"},{"key":"10.1016\/j.jviromet.2012.10.017_bib0010","doi-asserted-by":"crossref","first-page":"953","DOI":"10.3201\/eid1005.030908","article-title":"Human and porcine hepatitis E virus strains, United Kingdom","volume":"10","author":"Banks","year":"2004","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0015","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1111\/j.1865-1682.2011.01285.x","article-title":"Seroprevalence evolution of selected pathogens in Iberian wild boar","volume":"5","author":"Boadella","year":"2012","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0020","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1051\/vetres:2008017","article-title":"Estimation of hepatitis E virus transmission among pigs due to contact-exposure","volume":"39","author":"Bouwknegt","year":"2008","journal-title":"Vet. Res."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0025","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1007\/s11626-997-0061-y","article-title":"Three-dimensional endothelial-tumor epithelial cell interactions in human cervical cancers","volume":"33","author":"Chopra","year":"1997","journal-title":"In Vitro Cell. Dev. Biol. Anim."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0030","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1080\/15287390500361792","article-title":"Alterations in the virulence potential of enteric pathogens and bacterial-host cell interactions under simulated microgravity conditions","volume":"69","author":"Chopra","year":"2006","journal-title":"J. Toxicol. Environ. Health A"},{"key":"10.1016\/j.jviromet.2012.10.017_bib0035","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1086\/655898","article-title":"Pig liver sausage as a source of hepatitis E virus transmission to humans","volume":"202","author":"Colson","year":"2010","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0040","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.3201\/eid1711.110428","article-title":"Hepatitis E virus in rabbits, Virginia, USA","volume":"17","author":"Cossaboom","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0045","doi-asserted-by":"crossref","first-page":"2098","DOI":"10.1111\/j.1462-2920.2008.01631.x","article-title":"Use of the rotating wall vessel technology to study the effect of shear stress on growth behaviour of Pseudomonas aeruginosa PA01","volume":"10","author":"Crabbe","year":"2008","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0050","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1016\/S1473-3099(08)70255-X","article-title":"Hepatitis E: an emerging infection in developed countries","volume":"8","author":"Dalton","year":"2008","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0055","series-title":"Hepevirus","author":"Emerson","year":"2004"},{"key":"10.1016\/j.jviromet.2012.10.017_bib0060","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1086\/432488","article-title":"Thermal stability of hepatitis E virus","volume":"192","author":"Emerson","year":"2005","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0065","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1002\/rmv.384","article-title":"Hepatitis E virus","volume":"13","author":"Emerson","year":"2003","journal-title":"Rev. Med. Virol."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0070","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.ijfoodmicro.2007.11.068","article-title":"Inactivation of infectious hepatitis E virus present in commercial pig livers sold in local grocery stores in the United States","volume":"123","author":"Feagins","year":"2008","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0075","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1016\/j.eimc.2010.01.010","article-title":"Prevalence of hepatitis E virus (HEV) and risk factors in pig workers and blood donors","volume":"28","author":"Galiana","year":"2010","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0080","doi-asserted-by":"crossref","first-page":"181","DOI":"10.3181\/00379727-202-43525","article-title":"Rotating-wall vessel coculture of small intestine as a prelude to tissue modeling: aspects of simulated microgravity","volume":"202","author":"Goodwin","year":"1993","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0085","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jviromet.2005.07.004","article-title":"A broadly reactive one-step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus","volume":"131","author":"Jothikumar","year":"2006","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2012.10.017_bib0090","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.1128\/JCM.41.4.1548-1557.2003","article-title":"Broadly reactive and highly sensitive assay for Norwalk-like viruses based on real-time quantitative reverse transcription-PCR","volume":"41","author":"Kageyama","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0095","first-page":"55","article-title":"Clinostats and bioreactors","volume":"14","author":"Klaus","year":"2001","journal-title":"Gravit. Space Biol. Bull."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0100","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.ijfoodmicro.2010.02.016","article-title":"Hepatitis E virus load in swine organs and tissues at slaughterhouse determined by real-time RT-PCR","volume":"139","author":"Leblanc","year":"2010","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0105","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.virusres.2008.06.005","article-title":"Mutational events during the primary propagation and consecutive passages of hepatitis E virus strain JE03-1760F in cell culture","volume":"137","author":"Lorenzo","year":"2008","journal-title":"Virus Res."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0110","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1007\/s12560-011-9062-9","article-title":"Design and application of nucleic acid standards for quantitative detection of enteric viruses by real-time PCR","volume":"3","author":"Martinez-Martinez","year":"2011","journal-title":"Food Environ. Virol."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0115","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1128\/AEM.02428-06","article-title":"Novel quantitative biosystem for modeling physiological fluid shear stress on cells","volume":"73","author":"Nauman","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0125","doi-asserted-by":"crossref","first-page":"7172","DOI":"10.2741\/3219","article-title":"Hepatitis E: a curious zoonosis","volume":"13","author":"Pavio","year":"2008","journal-title":"Front. Biosci."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0135","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/j.jhep.2007.12.008","article-title":"Hepatitis E: an emerging awareness of an old disease","volume":"48","author":"Purcell","year":"2008","journal-title":"J. Hepatol."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0140","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.3201\/eid1712.110482","article-title":"Hepatitis e virus in rats, Los Angeles, CA, USA","volume":"17","author":"Purcell","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0145","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1128\/JVI.5.2.188-193.1970","article-title":"Functional heterogeneity of virions in human adenovirus types 2 and 12","volume":"5","author":"Rainbow","year":"1970","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0150","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1186\/1743-422X-8-487","article-title":"Thermal stability of hepatitis E virus assessed by a molecular biological approach","volume":"8","author":"Schielke","year":"2011","journal-title":"Virol. J."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0155","doi-asserted-by":"crossref","first-page":"396","DOI":"10.3201\/eid1303.060549","article-title":"In vitro cell culture infectivity assay for human noroviruses","volume":"13","author":"Straub","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0165","doi-asserted-by":"crossref","first-page":"3671","DOI":"10.1128\/JCM.01086-07","article-title":"Prolonged fecal shedding of hepatitis E virus (HEV) during sporadic acute hepatitis E: evaluation of infectivity of HEV in fecal specimens in a cell culture system","volume":"45","author":"Takahashi","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0170","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/0042-6822(91)90760-9","article-title":"Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome","volume":"185","author":"Tam","year":"1991","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2012.10.017_bib0175","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1099\/vir.0.82535-0","article-title":"Development and evaluation of an efficient cell-culture system for Hepatitis E virus","volume":"88","author":"Tanaka","year":"2007","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0180","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1099\/vir.0.81661-0","article-title":"Molecular tracing of Japan-indigenous hepatitis E viruses","volume":"87","author":"Tanaka","year":"2006","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0185","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1002\/jmv.20147","article-title":"Consumption of uncooked deer meat as a risk factor for hepatitis E virus infection: an age- and sex-matched case\u2013control study","volume":"74","author":"Tei","year":"2004","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0190","first-page":"181","article-title":"HEV prevalence in the general population and among workers at zoonotic risk in Latium Region","volume":"19","author":"Vulcano","year":"2007","journal-title":"Ann. Ig."},{"key":"10.1016\/j.jviromet.2012.10.017_bib0195","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1128\/AEM.17.5.658-664.1969","article-title":"Evaluation of factors related to growth of Rift Valley fever virus in suspended cell cultures","volume":"17","author":"Walker","year":"1969","journal-title":"Appl. Microbiol."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093412003813?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093412003813?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T03:34:13Z","timestamp":1594870453000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093412003813"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["S0166093412003813"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2012.10.017","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2013,2]]}}}