{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T12:43:44Z","timestamp":1759063424060},"reference-count":13,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000774","name":"Defense Threat Reduction Agency (DTRA)","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000774","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2013,10]]},"DOI":"10.1016\/j.jviromet.2013.06.007","type":"journal-article","created":{"date-parts":[[2013,6,10]],"date-time":"2013-06-10T08:48:19Z","timestamp":1370854099000},"page":"226-231","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"title":["Validation of a cell-based ELISA as a screening tool identifying anti-alphavirus small-molecule inhibitors"],"prefix":"10.1016","volume":"193","author":[{"given":"Kevin B.","family":"Spurgers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clarence R.","family":"Hurt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey W.","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lori T.","family":"Eccelston","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cathleen M.","family":"Lind","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishwanath R.","family":"Lingappa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela J.","family":"Glass","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2013.06.007_bib0010","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1089\/vbz.2006.6.115","article-title":"The 2005\u20132006 Chikungunya epidemic in the Indian Ocean","volume":"6","author":"Higgs","year":"2006","journal-title":"Vector Borne Zoonotic Dis"},{"key":"10.1016\/j.jviromet.2013.06.007_bib0015","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1128\/JVI.01864-10","article-title":"Role of the vacuolar-ATPase in Sindbis virus infection","volume":"85","author":"Hunt","year":"2011","journal-title":"Journal of Virology"},{"key":"10.1016\/j.jviromet.2013.06.007_bib0020","doi-asserted-by":"crossref","first-page":"3207","DOI":"10.1016\/j.bmcl.2005.05.013","article-title":"Design, synthesis, and evaluation of dioxane-based antiviral agents targeted against the Sindbis virus capsid protein","volume":"15","author":"Kim","year":"2005","journal-title":"Bioorganic and Medicinal Chemistry Letters"},{"key":"10.1016\/j.jviromet.2013.06.007_bib0025","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0166-3542(99)00011-X","article-title":"Guanosine nucleotide analogs as inhibitors of alphavirus mRNA capping enzyme","volume":"42","author":"Lampio","year":"1999","journal-title":"Antiviral Research"},{"key":"10.1016\/j.jviromet.2013.06.007_bib0030","doi-asserted-by":"crossref","first-page":"12683","DOI":"10.1128\/JVI.01345-10","article-title":"Antibody to the E3 glycoprotein protects mice against lethal venezuelan equine encephalitis virus infection","volume":"84","author":"Parker","year":"2010","journal-title":"Journal of Virology"},{"key":"10.1016\/j.jviromet.2013.06.007_bib0035","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1086\/597275","article-title":"Identification of thieno[3,2-b]pyrrole derivatives as novel small molecule inhibitors of neurotropic alphaviruses","volume":"199","author":"Peng","year":"2009","journal-title":"Journal of Infectious Diseases"},{"key":"10.1016\/j.jviromet.2013.06.007_bib0040","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.antiviral.2008.01.001","article-title":"A luciferase-based screening method for inhibitors of alphavirus replication applied to nucleoside analogues","volume":"78","author":"Pohjala","year":"2008","journal-title":"Antiviral Research"},{"key":"10.1016\/j.jviromet.2013.06.007_bib0045","first-page":"493","article-title":"A simple method of estimating fifty percent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"American Journal of Hygiene"},{"key":"10.1016\/j.jviromet.2013.06.007_bib0050","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1089\/bsp.2009.0032","article-title":"Alphavirus antiviral drug development: scientific gap analysis and prospective research areas","volume":"7","author":"Reichert","year":"2009","journal-title":"Biosecurity and Bioterrorism"},{"key":"10.1016\/j.jviromet.2013.06.007_bib0055","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/0042-6822(85)90343-5","article-title":"The neutralization site on the E2 glycoprotein of Venezuelan equine encephalomyelitis (TC-83) virus is composed of multiple conformationally stable epitopes","volume":"142","author":"Roehrig","year":"1985","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2013.06.007_bib0060","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1038\/nrmicro2368","article-title":"Biology and pathogenesis of chikungunya virus","volume":"8","author":"Schwartz","year":"2010","journal-title":"Nature Reviews Microbiology"},{"key":"10.1016\/j.jviromet.2013.06.007_bib0065","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1128\/MMBR.58.3.491-562.1994","article-title":"The alphaviruses: gene expression, replication, and evolution","volume":"58","author":"Strauss","year":"1994","journal-title":"Microbiological Reviews"},{"key":"10.1016\/j.jviromet.2013.06.007_bib0070","doi-asserted-by":"crossref","first-page":"e623","DOI":"10.1371\/journal.pntd.0000623","article-title":"Chikungunya: a potentially emerging epidemic?","volume":"4","author":"Thiboutot","year":"2010","journal-title":"PLOS Neglected Tropical Diseases"}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093413002206?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093413002206?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T03:03:11Z","timestamp":1595905391000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093413002206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["S0166093413002206"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2013.06.007","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2013,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Validation of a cell-based ELISA as a screening tool identifying anti-alphavirus small-molecule inhibitors","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2013.06.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}