{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T12:37:10Z","timestamp":1777811830594,"version":"3.51.4"},"reference-count":23,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100012164","name":"National High-tech Research and Development Program","doi-asserted-by":"publisher","award":["2011AA10A212"],"award-info":[{"award-number":["2011AA10A212"]}],"id":[{"id":"10.13039\/501100012164","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30800831"],"award-info":[{"award-number":["30800831"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31121004"],"award-info":[{"award-number":["31121004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2013JC001"],"award-info":[{"award-number":["2013JC001"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2013,12]]},"DOI":"10.1016\/j.jviromet.2013.08.005","type":"journal-article","created":{"date-parts":[[2013,8,12]],"date-time":"2013-08-12T14:15:59Z","timestamp":1376316959000},"page":"33-38","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"title":["Methods for detecting ATP hydrolysis and nucleic acid unwinding of Japanese encephalitis virus NS3 helicase"],"prefix":"10.1016","volume":"194","author":[{"given":"Jin\u2019e","family":"Fang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guiqing","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengbo","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. Fu","family":"Zhen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanchun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunfeng","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2013.08.005_bib0005","series-title":"Encyclopaedia of Virology","first-page":"182","article-title":"Japanese encephalitis virus","author":"Barrett","year":"2008"},{"key":"10.1016\/j.jviromet.2013.08.005_bib0010","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.febslet.2004.04.072","article-title":"Direct fluorometric measurement of hepatitis C virus helicase activity","volume":"567","author":"Boguszewska-Chachulska","year":"2004","journal-title":"FEBS Lett."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0015","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1046\/j.1432-1327.1999.00854.x","article-title":"Biochemical properties of a minimal functional domain with ATP-binding activity of the NTPase\/helicase of hepatitis C virus","volume":"266","author":"Borowski","year":"1999","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0020","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1046\/j.1432-1033.2003.03540.x","article-title":"Halogenated benzimidazoles and benzotriazoles as inhibitors of the NTPase\/helicase activities of hepatitis C and related viruses","volume":"270","author":"Borowski","year":"2003","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0025","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1128\/AAC.02251-12","article-title":"A novel benzoxazole inhibitor of dengue virus replication that targets the NS3 helicase","volume":"57","author":"Byrd","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0030","first-page":"363","article-title":"The development, optimization and validation of an assay for high throughput antiviral drug screening against dengue virus","volume":"2","author":"Che","year":"2009","journal-title":"Int. J. Clin. Exp. Med."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0035","doi-asserted-by":"crossref","first-page":"e36244","DOI":"10.1371\/journal.pone.0036244","article-title":"Novel ATP-independent RNA annealing activity of the dengue virus NS3 helicase","volume":"7","author":"Gebhard","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jviromet.2013.08.005_bib0040","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.antiviral.2007.10.005","article-title":"Does antiviral therapy have a role in the control of Japanese encephalitis?","volume":"78","author":"Gould","year":"2008","journal-title":"Antiviral Res."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0045","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1056\/NEJM198809083191004","article-title":"Protection against Japanese encephalitis by inactivated vaccines","volume":"319","author":"Hoke","year":"1988","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0050","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1002\/jmv.1890440204","article-title":"Homologous and heterologous neutralization antibody responses after immunization with Japanese encephalitis vaccine among Taiwan children","volume":"44","author":"Ku","year":"1994","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0055","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nrd1853","article-title":"Viral and cellular RNA helicases as antiviral targets","volume":"4","author":"Kwong","year":"2005","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0060","doi-asserted-by":"crossref","first-page":"3209","DOI":"10.1038\/emboj.2008.232","article-title":"Insights into RNA unwinding and ATP hydrolysis by the flavivirus NS3 protein","volume":"27","author":"Luo","year":"2008","journal-title":"EMBO J."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0065","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1128\/JVI.01788-07","article-title":"Crystal structure of the NS3 protease-helicase from dengue virus","volume":"82","author":"Luo","year":"2008","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0070","doi-asserted-by":"crossref","first-page":"S98","DOI":"10.1038\/nm1144","article-title":"Emerging flaviviruses: the spread and resurgence of Japanese encephalitis, West Nile and dengue viruses","volume":"10","author":"Mackenzie","year":"2004","journal-title":"Nat. Med."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0075","doi-asserted-by":"crossref","first-page":"1884","DOI":"10.1093\/jac\/dks147","article-title":"Ivermectin is a potent inhibitor of flavivirus replication specifically targeting NS3 helicase activity: new prospects for an old drug","volume":"67","author":"Mastrangelo","year":"2012","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0080","doi-asserted-by":"crossref","first-page":"9633","DOI":"10.1128\/JVI.75.20.9633-9643.2001","article-title":"Mutagenesis of the dengue virus type 2 NS3 protein within and outside helicase motifs: effects on enzyme activity and virus replication","volume":"75","author":"Matusan","year":"2001","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0085","first-page":"1649","article-title":"Mutational analysis of hepatitis C virus NS3-associated helicase","volume":"81","author":"Paolini","year":"2000","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0090","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.antiviral.2008.08.004","article-title":"Molecular targets for flavivirus drug discovery","volume":"81","author":"Sampath","year":"2009","journal-title":"Antiviral Res."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0095","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/0042-6822(87)90144-9","article-title":"Complete nucleotide sequence of the Japanese encephalitis virus genome RNA","volume":"161","author":"Sumiyoshi","year":"1987","journal-title":"Virology"},{"issue":"Suppl. 2","key":"10.1016\/j.jviromet.2013.08.005_bib0100","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0264-410X(00)00037-2","article-title":"New initiatives for the control of Japanese encephalitis by vaccination: minutes of a WHO\/CVI meeting, Bangkok, Thailand, 13\u201315 October 1998","volume":"18","author":"Tsai","year":"2000","journal-title":"Vaccine"},{"key":"10.1016\/j.jviromet.2013.08.005_bib0105","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/j.febslet.2009.01.008","article-title":"Analysis of the nucleoside triphosphatase, RNA triphosphatase, and unwinding activities of the helicase domain of dengue virus NS3 protein","volume":"583","author":"Wang","year":"2009","journal-title":"FEBS Lett."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0110","doi-asserted-by":"crossref","first-page":"214","DOI":"10.4269\/ajtmh.1988.39.214","article-title":"Safety of a live-attenuated Japanese encephalitis virus vaccine (SA14-14-2) for children","volume":"39","author":"Xin","year":"1988","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.jviromet.2013.08.005_bib0115","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1128\/JVI.79.16.10278-10288.2005","article-title":"Structure of the Dengue virus helicase\/nucleoside triphosphatase catalytic domain at a resolution of 2.4A","volume":"79","author":"Xu","year":"2005","journal-title":"J. Virol."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093413003376?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093413003376?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:04:27Z","timestamp":1777496667000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093413003376"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12]]},"references-count":23,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["S0166093413003376"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2013.08.005","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2013,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Methods for detecting ATP hydrolysis and nucleic acid unwinding of Japanese encephalitis virus NS3 helicase","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2013.08.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}