{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T23:32:20Z","timestamp":1768692740588,"version":"3.49.0"},"reference-count":27,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2014,11,1]],"date-time":"2014-11-01T00:00:00Z","timestamp":1414800000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"National Fund for Basic, Strategic and Frontier Application Research in Agriculture (NFBSFARA), Indian Council of Agricultural Research (ICAR)"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2014,11]]},"DOI":"10.1016\/j.jviromet.2014.07.027","type":"journal-article","created":{"date-parts":[[2014,7,28]],"date-time":"2014-07-28T21:03:38Z","timestamp":1406581418000},"page":"6-10","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Development of a SYBR Green I based duplex real-time PCR for detection of bovine herpesvirus-1 in semen"],"prefix":"10.1016","volume":"208","author":[{"given":"Sachin S.","family":"Pawar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chetan D.","family":"Meshram","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niraj K.","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohini","family":"Saini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.P.","family":"Mishra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6969-3509","authenticated-orcid":false,"given":"Praveen K.","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2014.07.027_bib0005","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.mcp.2011.03.001","article-title":"Development of a SYBR Green I based real-time RT-PCR assay for detection and quanti\ufb01cation of bovine coronavirus","volume":"25","author":"Amer","year":"2011","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.jviromet.2014.07.027_bib0010","first-page":"467","article-title":"Isolation of BHV-1 from bovine semen and application of real-time PCR for diagnosis of IBR\/IPV from clinical samples","volume":"80","author":"Chandranaik","year":"2010","journal-title":"Vet. Arhiv."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0015","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.jviromet.2011.04.013","article-title":"Detection and differentiation of bovine herpesvirus 1 and 5 using a multiplex real-time polymerase chain reaction","volume":"175","author":"Diallo","year":"2011","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2014.07.027_bib0020","doi-asserted-by":"crossref","first-page":"215","DOI":"10.20506\/rst.16.1.1017","article-title":"Disease risks to animal health from artificial insemination with bovine semen","volume":"16","author":"Eaglesome","year":"1997","journal-title":"Rev. Sci. Tech. Off. Int. Epiz."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0025","first-page":"317","article-title":"Bovine herpesviruses. Part 1","volume":"47","author":"Gibbs","year":"1977","journal-title":"Vet. Bull."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0030","first-page":"889","article-title":"Restriction enzyme analysis of bovine herpesvirus-1 DNA from an Indian isolate","volume":"29","author":"Gupta","year":"1993","journal-title":"Biochem. Mol. Biol. Int."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0035","first-page":"23","article-title":"Rapid and sensitive PCR-based test for detection of bovine herpesvirus-1 in semen","volume":"17","author":"Gupta","year":"2006","journal-title":"Indian J. Virol."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0040","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1128\/JCM.42.5.1863-1868.2004","article-title":"Practical considerations in design of internal amplification controls for diagnostic PCR assays","volume":"42","author":"Hoorfar","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0045","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.jviromet.2010.11.020","article-title":"Multiplex real-time RT-PCR detection of three viruses associated with the bovine respiratory disease complex","volume":"171","author":"Horwood","year":"2011","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2014.07.027_bib0050","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.jviromet.2004.03.006","article-title":"Differential amplification and quantitation of Marek's disease viruses using real-time polymerase chain reaction","volume":"119","author":"Islam","year":"2004","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2014.07.027_bib0055","doi-asserted-by":"crossref","first-page":"3185","DOI":"10.1016\/S0264-410X(00)00106-7","article-title":"Analysis of latency in cattle after inoculation with a temperature sensitive mutant of bovine herpesvirus 1 (RLB106)","volume":"18","author":"Jones","year":"2000","journal-title":"Vaccine"},{"key":"10.1016\/j.jviromet.2014.07.027_bib0060","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.jbiotec.2005.11.014","article-title":"Absolute and relative QPCR quanti\ufb01cation of plasmid copy number in Escherichia coli","volume":"123","author":"Lee","year":"2006","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0065","first-page":"70","article-title":"IBR-keratoconjunctivitis in calves","volume":"1","author":"Mehrotra","year":"1976","journal-title":"Indian J. Vet. Pathol."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0070","series-title":"OIE Terrestrial Manual","article-title":"Infectious bovine rhinotracheitis\/infectious pustular vulvovaginitis","author":"OIE","year":"2010"},{"key":"10.1016\/j.jviromet.2014.07.027_bib0075","first-page":"313","article-title":"Use of real-time polymerase chain reaction to detect bovine herpesvirus 1 in frozen cattle and buffalo semen in India","volume":"47","author":"Rana","year":"2011","journal-title":"Vet. Ital."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0080","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-1135(98)00213-2","article-title":"A high sensitivity nested PCR assay for BHV-1 detection in semen of naturally infected bulls","volume":"63","author":"Rocha","year":"1998","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0085","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.jviromet.2012.01.018","article-title":"Development of SYBR Green I based real-time PCR assays for quantitative detection of Rice tungro bacilliform virus and Rice tungro spherical virus","volume":"181","author":"Sharma","year":"2012","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2014.07.027_bib0090","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/1746-6148-8-37","article-title":"One-step multiplex real-time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3","volume":"8","author":"Thonur","year":"2012","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0095","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/BF03402063","article-title":"BoHV-1: new molecular approaches to control a common and widespread infection","volume":"5","author":"Turin","year":"1999","journal-title":"Mol. Med."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0100","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1080\/01652176.1995.9694526","article-title":"Bovine herpes virus 1 in semen of bulls and the risk of transmission: a brief review","volume":"17","author":"Van der Oirschot","year":"1995","journal-title":"Vet. Q."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0105","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1136\/vr.132.2.32","article-title":"A subclinical infection of bulls with bovine herpesvirus type 1 at an artificial insemination centre","volume":"132","author":"Van der Oirschot","year":"1993","journal-title":"Vet. Rec."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0110","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1099\/0022-1317-76-9-2387","article-title":"A glycoprotein E deletion mutant of bovine herpesvirus 1 infects the same limited number of tissues in calves as wild-type virus, but for a shorter period","volume":"76","author":"Van Engelenburg","year":"1995","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0115","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.vetmic.2007.06.005","article-title":"An international inter-laboratory ring trial to evaluate a real-time PCR assay for the detection of bovine herpesvirus 1 in extended bovine semen","volume":"126","author":"Wang","year":"2008","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.jviromet.2014.07.027_bib0120","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.jviromet.2007.04.002","article-title":"Validation of a real-time PCR assay for the detection of bovine herpesvirus 1 in bovine semen","volume":"144","author":"Wang","year":"2007","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2014.07.027_bib0125","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.jviromet.2011.03.028","article-title":"Development and validation of a triplex real-time PCR assay for the rapid detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of bovine herpesvirus type 1","volume":"174","author":"Wernike","year":"2011","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2014.07.027_bib0130","series-title":"Herpesvirus Diseases of Cattle, Horses, and Pigs","article-title":"Infectious bovine rhinotracheitis\/vulvovaginitis (BoHV-1)","author":"Wyler","year":"1989"},{"key":"10.1016\/j.jviromet.2014.07.027_bib0135","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/0034-5288(95)90058-6","article-title":"The use of a polymerase chain reaction assay for the detection of bovine herpesvirus 1 in the semen of experimentally infected bulls by dot blot hybridization, polymerase chain reaction and virus isolation","volume":"59","author":"Xia","year":"1995","journal-title":"Res. Vet. Sci."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093414002997?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093414002997?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,8,13]],"date-time":"2019-08-13T04:24:57Z","timestamp":1565670297000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093414002997"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11]]},"references-count":27,"alternative-id":["S0166093414002997"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2014.07.027","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2014,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a SYBR Green I based duplex real-time PCR for detection of bovine herpesvirus-1 in semen","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2014.07.027","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}