{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:25:53Z","timestamp":1759332353932},"reference-count":27,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T00:00:00Z","timestamp":1438387200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Cooperation Project for Science and Technology Department of Guizhou Province and the University","award":["LH20147670"],"award-info":[{"award-number":["LH20147670"]}]},{"name":"Project for Agricultural Science and Technology of Guizhou Province","award":["NY20103042"],"award-info":[{"award-number":["NY20103042"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2015,8]]},"DOI":"10.1016\/j.jviromet.2015.04.005","type":"journal-article","created":{"date-parts":[[2015,4,15]],"date-time":"2015-04-15T18:41:08Z","timestamp":1429123268000},"page":"21-26","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Construction of a novel porcine circovirus type 2 infectious clone as a basis for the development of a PCV2 iDNA vaccine"],"prefix":"10.1016","volume":"220","author":[{"given":"Wei-Cheng","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-Yong","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"De-yuan","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai-Ying","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen-Jiang","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2015.04.005_bib0005","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1177\/104063870001200102","article-title":"Porcine circoviruses: a review","volume":"12","author":"Allan","year":"2000","journal-title":"J. Vet. Diagn. Investig."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0010","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.virusres.2011.09.041","article-title":"Efficacy and future prospects of commercially available and experimental vaccines against porcine circovirus type 2 (PCV2)","volume":"164","author":"Beach","year":"2012","journal-title":"Virus Res."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0015","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.vaccine.2010.10.050","volume":"29","author":"Beach","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.jviromet.2015.04.005_bib0020","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.tvjl.2012.06.031","article-title":"Commercial porcine circovirus type 2 vaccines: efficacy and clinical application","volume":"194","author":"Chae","year":"2012","journal-title":"Vet. J."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0025","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.tvjl.2004.01.012","article-title":"A review of porcine circovirus 2-associated syndromes and diseases","volume":"169","author":"Chae","year":"2005","journal-title":"Vet. J."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0030","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/s11259-011-9461-2","article-title":"Viral distribution and lesions in Kunming mice experimentally infected with porcine circovirus type 2b","volume":"35","author":"Deng","year":"2011","journal-title":"Vet. Res. Commun."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0035","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1128\/JVI.76.2.541-551.2002","article-title":"Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease, virus distribution, and pathologic lesions","volume":"76","author":"Fenaux","year":"2002","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0040","doi-asserted-by":"crossref","first-page":"6297","DOI":"10.1128\/JVI.78.12.6297-6303.2004","article-title":"A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs","volume":"78","author":"Fenaux","year":"2004","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0045","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1111\/j.1939-1676.2009.0389.x","article-title":"Porcine circovirus type 2 and porcine circovirus-associated disease","volume":"23","author":"Gillespie","year":"2009","journal-title":"J. Vet. Intern. Med."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0050","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1186\/1743-422X-7-273","article-title":"Porcine circovirus type 2 (PCV2): genetic variation and newly emerging genotypes in China","volume":"7","author":"Guo","year":"2010","journal-title":"Virol. J."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0055","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1186\/1743-422X-8-437","article-title":"Genetic characterization of porcine circovirus 2 found in Malaysia","volume":"8","author":"Jaganathan","year":"2011","journal-title":"Virol. J."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0060","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1354\/vp.38-1-74","article-title":"Viral replication and lesions in BALB\/c mice experimentally inoculated with porcine circovirus isolated from a pig with postweaning multisystemic wasting disease","volume":"38","author":"Kiupel","year":"2001","journal-title":"Vet. Pathol."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0065","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1746-6148-1-7","article-title":"Porcine circovirus type 2 (PCV2) causes apoptosis in experimentally inoculated BALB\/c mice","volume":"1","author":"Kiupel","year":"2005","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0070","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1186\/1743-422X-7-158","article-title":"A mouse model to study infection against porcine circovirus type 2: viral distribution and lesions in mouse","volume":"7","author":"Li","year":"2010","journal-title":"Virol. J."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0075","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1292\/jvms.66.237","article-title":"Development of an ELISA based on the baculovirus-expressed capsid protein of porcine circovirus type 2 as antigen","volume":"66","author":"Liu","year":"2004","journal-title":"J. Vet. Med. Sci."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0080","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.virusres.2007.03.026","article-title":"Construction and characterization of porcine circovirus type 2 carrying a genetic marker strain","volume":"127","author":"Liu","year":"2007","journal-title":"Virus Res."},{"issue":"7","key":"10.1016\/j.jviromet.2015.04.005_bib0085","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1016\/j.vaccine.2008.11.105","article-title":"Comparison of efficacy of commercial one dose and two dose PCV2 vaccines using a mixed PRRSV\u2013PCV2\u2013SIV clinical infection model 2\u20133-months post vaccination","volume":"27","author":"Opriessnig","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.jviromet.2015.04.005_bib0090","doi-asserted-by":"crossref","DOI":"10.1099\/vir.0.066423-0","article-title":"The mutant U.S. strain of porcine circovirus type 2 (mPCV2) exhibits similar virulence to the classical PCV2a and PCV2b strains in caesarian-derived colostrum-deprived pigs","author":"Opriessnig","year":"2014","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0095","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.virusres.2011.10.007","article-title":"Porcine circovirus type 2 (PCV2) infections: clinical signs, pathology and laboratory diagnosis","volume":"164","author":"Segal\u00e9s","year":"2012","journal-title":"Virus Res."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0100","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.vetmic.2012.12.033","article-title":"The natural history of porcine circovirus type 2: from an inoffensive virus to a devastating swine disease?","volume":"165","author":"Segal\u00e9s","year":"2013","journal-title":"Vet. Microbiol."},{"issue":"52","key":"10.1016\/j.jviromet.2015.04.005_bib0105","doi-asserted-by":"crossref","first-page":"7313","DOI":"10.1016\/j.vaccine.2009.09.084","article-title":"A genetically engineered chimeric vaccine against porcine circovirus type 2 (PCV2) improves clinical, pathological and virological outcomes in postweaning multisystemic wasting syndrome affected farms","volume":"27","author":"Segales","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.jviromet.2015.04.005_bib0110","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1016\/j.vaccine.2012.12.050","article-title":"Novel vaccine against Venezuelan equine encephalitis combines advantages of DNA immunization and a live attenuated vaccine","volume":"31","author":"Tretyakova","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.jviromet.2015.04.005_bib0115","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.virol.2014.07.050","article-title":"Plasmid DNA initiates replication of yellow fever vaccine in vitro and elicits virus-specific immune response in mice","volume":"468\u2013470C","author":"Tretyakova","year":"2014","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2015.04.005_bib0120","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/BF01310989","article-title":"Replication of porcine circovirus: induction by glucosamine and cell cycle dependence","volume":"96","author":"Tischer","year":"1987","journal-title":"Arch. Virol."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0125","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.virusres.2009.05.015","article-title":"Genetic variation analysis of Chinese strains of porcine circovirus type 2","volume":"145","author":"Wang","year":"2009","journal-title":"Virus Res."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0130","first-page":"90","article-title":"Cloning and sequence analysis of the complete genome of porcine circovirus type 2 from Guizhou province","volume":"46","author":"Wang","year":"2014","journal-title":"Anim. Sci. Vet. Med."},{"key":"10.1016\/j.jviromet.2015.04.005_bib0135","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.jviromet.2014.08.001","article-title":"Establishment and application of a multiplex PCR for rapid and simultaneous detection of six viruses in swine","volume":"208","author":"Zeng","year":"2014","journal-title":"J. Virol. Methods"}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016609341500141X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016609341500141X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,9,23]],"date-time":"2018-09-23T09:51:29Z","timestamp":1537696289000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S016609341500141X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8]]},"references-count":27,"alternative-id":["S016609341500141X"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2015.04.005","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2015,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Construction of a novel porcine circovirus type 2 infectious clone as a basis for the development of a PCV2 iDNA vaccine","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2015.04.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}