{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:11:24Z","timestamp":1762866684642},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2015,10]]},"DOI":"10.1016\/j.jviromet.2015.07.022","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T01:51:27Z","timestamp":1438912287000},"page":"105-108","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Two unusual hepatitis C virus subtypes, 2j and 2q, in Spain: Identification by nested-PCR and sequencing of a NS5B region"],"prefix":"10.1016","volume":"223","author":[{"given":"N.","family":"Margall","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"March","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Espa\u00f1ol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Torras","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Gallego","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Coll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2015.07.022_bib0050","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1002\/jmv.23545","article-title":"Hepatitis C virus subtyping based on sequencing of the C\/E1 and NS5B genomic regions in comparison to a commercially available line probe assay","volume":"85","author":"Av\u00f3","year":"2013","journal-title":"J. Med. Virol."},{"issue":"3","key":"10.1016\/j.jviromet.2015.07.022_bib0010","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1157\/13090276","article-title":"Hepatitis C in Spain","volume":"172","author":"Bruguera","year":"2006","journal-title":"Med. Cl\u00edn."},{"key":"10.1016\/j.jviromet.2015.07.022_bib0035","doi-asserted-by":"crossref","first-page":"347","DOI":"10.3892\/ijmm.2012.1209","article-title":"Comparison of three different HCV genotyping methods: core, NS5B sequence analysis and line probe assay","volume":"31","author":"Cai","year":"2013","journal-title":"Int. J. Mol. Med."},{"key":"10.1016\/j.jviromet.2015.07.022_bib0130","doi-asserted-by":"crossref","first-page":"5813","DOI":"10.3748\/wjg.v19.i35.5813","article-title":"Molecular epidemiology and putative origin of hepatitis C virus in random volunteers from Argentina","volume":"19","author":"del Pino","year":"2013","journal-title":"World J. Gastroenterol."},{"key":"10.1016\/j.jviromet.2015.07.022_bib0100","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1157\/13083370","article-title":"Follow up of the prevalence of hepatitis C virus genotypes in Spain during a nine-year period (1996\u20132004)","volume":"24","author":"Echevarria","year":"2006","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"issue":"10","key":"10.1016\/j.jviromet.2015.07.022_bib0070","doi-asserted-by":"crossref","first-page":"2516","DOI":"10.1128\/JCM.34.10.2516-2521.1996","article-title":"Comparative study of three methods for genotyping hepatitis c virus strains in samples from Spanish patients","volume":"34","author":"Forns","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2015.07.022_bib0045","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/j.jcv.2013.09.010","article-title":"Accuracy of a commercially available assay for HCV genotyping and subtyping in the clinical practice","volume":"58","author":"Gonz\u00e1lez","year":"2013","journal-title":"J. Clin. Virol."},{"issue":"2","key":"10.1016\/j.jviromet.2015.07.022_bib0110","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1002\/(SICI)1096-9071(199806)55:2<92::AID-JMV2>3.0.CO;2-I","article-title":"Evidence for high genetic diversity and long-term endemicity of hepatitis C virus genotypes 1 and 2 in West Africa","volume":"55","author":"Jeannel","year":"1998","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jviromet.2015.07.022_bib0125","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.1002\/jmv.23674","article-title":"Evidence for two phylogenetic clusters within hepatitis C virus (HCV) Genotype 2 inferred from analysis of complete coding sequences of 15 HCV strains","volume":"85","author":"Jordier","year":"2013","journal-title":"J. Med. Virol."},{"first-page":"1014","year":"2012","series-title":"Virus Taxonomy. Ninth report of the international Committee on Taxonomy of viruses","key":"10.1016\/j.jviromet.2015.07.022_bib0015"},{"key":"10.1016\/j.jviromet.2015.07.022_bib0020","first-page":"33","article-title":"Nomenclature and numbering of the hepatitis C virus","volume":"vol. 510","author":"Kuiken","year":"2009"},{"key":"10.1016\/j.jviromet.2015.07.022_bib0065","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1093\/bioinformatics\/bth485","article-title":"The Los Alamos hepatitis C sequence database","volume":"21","author":"Kuiken","year":"2005","journal-title":"Bioinformatics"},{"key":"10.1016\/j.jviromet.2015.07.022_bib0025","series-title":"Fields virology","first-page":"1253","article-title":"Hepatitis C virus","author":"Lemon","year":"2007"},{"key":"10.1016\/j.jviromet.2015.07.022_bib0105","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1099\/vir.0.038315-0","article-title":"Full-length sequences of 11 hepatitis C virus genotype isolates representing five subtypes and six unclassified lineages with unique geographical distributions and genetic variation patterns","volume":"93","author":"Li","year":"2012","journal-title":"J. Gen. Virol."},{"issue":"12","key":"10.1016\/j.jviromet.2015.07.022_bib0090","doi-asserted-by":"crossref","first-page":"e1000198","DOI":"10.1371\/journal.pmed.1000198","article-title":"The global spread of Hepatitis C virus 1a and 1b: a phylodynamic and phylogeographic analysis","volume":"6","author":"Magiorkinis","year":"2009","journal-title":"PLoS Med."},{"key":"10.1016\/j.jviromet.2015.07.022_bib0140","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1002\/jmv.22054","article-title":"Hepatitis C virus sequences from different patients confirm the existence and transmissibility of Subtype 2q, a rare subtype circulating in the metropolitan area of Barcelona","volume":"83","author":"Martr\u00f3","year":"2011","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jviromet.2015.07.022_bib0040","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1128\/JCM.01623-07","article-title":"Evaluation of a new assay in comparison with reverse hybridization and sequencing methods for hepatitis C virus genotyping targeting both 5\u2032 non coding and nonstructural 5b genomic regions","volume":"46","author":"Martr\u00f3","year":"2008","journal-title":"J. Clin. Microbiol."},{"issue":"12","key":"10.1016\/j.jviromet.2015.07.022_bib0095","doi-asserted-by":"crossref","first-page":"e14324","DOI":"10.5812\/hepatmon.14324","article-title":"Distribution of hepatitis C virus genotypes among patients with hepatitis C virus infection in Hormozgan, Iran","volume":"13","author":"Mousavi","year":"2013","journal-title":"Hepat. Mon."},{"key":"10.1016\/j.jviromet.2015.07.022_bib0115","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1128\/JCM.02366-06","article-title":"Use of sequence analysis of the NS5B region for routine genotyping of Hepatitis C virus with reference to C\/E1 and 5\u2032 untranslated region sequences","volume":"45","author":"Murphy","year":"2007","journal-title":"J. Clin. Microbiol."},{"issue":"1","key":"10.1016\/j.jviromet.2015.07.022_bib0085","doi-asserted-by":"crossref","first-page":"e86413","DOI":"10.1371\/journal.pone.0086413","article-title":"HCV genotypes, characterization of mutations conferring drug resistance to protease inhibitors, and risk factors among blood donors in Sao Paulo, Brazil","volume":"9","author":"Nishiya","year":"2014","journal-title":"PLOS ONE"},{"issue":"6","key":"10.1016\/j.jviromet.2015.07.022_bib0075","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1111\/j.1469-0691.2008.01988.x","article-title":"Multiple genotypes and subtypes of hepatitis B and C viruses in Belarus: similarities with Russia and western European influences","volume":"14","author":"Olinger","year":"2008","journal-title":"Clin. Microbiol. Infect."},{"issue":"3","key":"10.1016\/j.jviromet.2015.07.022_bib0080","doi-asserted-by":"crossref","first-page":"429","DOI":"10.5578\/mb.7685","article-title":"Distribution of hepatitis C virus genotypes among patients with chronic hepatitis C infection in Akdeniz University Hospital, Antalya, Turkey: a five-year evaluation","volume":"48","author":"Saglik","year":"2014","journal-title":"Mikrobiyol. Bul."},{"key":"10.1016\/j.jviromet.2015.07.022_bib0055","doi-asserted-by":"crossref","first-page":"187193","DOI":"10.1016\/S0166-0934(03)00070-3","article-title":"Determining hepatitis C genotype by analyzing the sequence of the NS5b region","volume":"109","author":"Sandres Saune","year":"2003","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2015.07.022_bib0135","doi-asserted-by":"crossref","first-page":"e14315","DOI":"10.1371\/journal.pone.0014315","article-title":"Genetic history of hepatitis C virus in Venezuela: high diversity and long time of evolution of HCV genotype 2","volume":"5","author":"Sulbar\u00e1n","year":"2010","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jviromet.2015.07.022_bib0120","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1002\/jmv.20765","article-title":"Genetic diversity of HCV genotype 2 strains in South Western France","volume":"79","author":"Thomas","year":"2007","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jviromet.2015.07.022_bib0060","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1099\/0022-1317-79-8-1847","article-title":"The entire nucleotide sequences of three hepatitis C virus isolates in genetic groups 7\u20139 and comparison with those in the other eight genetic groups","volume":"79","author":"Tokita","year":"1998","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2015.07.022_bib0030","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1128\/JCM.02390-07","article-title":"Evaluation of versant hepatitis C virus genotype assay (LiPA) 2.0","volume":"46","author":"Verbeeck","year":"2008","journal-title":"J. Clin. Microbiol."},{"issue":"4","key":"10.1016\/j.jviromet.2015.07.022_bib0005","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1111\/jvh.12230","article-title":"Importance of HCV genotype 1 subtypes for drug resistance and response to therapy","volume":"21","author":"Wyles","year":"2014","journal-title":"J. Viral Hepat."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093415002657?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093415002657?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,6]],"date-time":"2020-09-06T23:26:25Z","timestamp":1599434785000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093415002657"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10]]},"references-count":28,"alternative-id":["S0166093415002657"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2015.07.022","relation":{},"ISSN":["0166-0934"],"issn-type":[{"type":"print","value":"0166-0934"}],"subject":[],"published":{"date-parts":[[2015,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Two unusual hepatitis C virus subtypes, 2j and 2q, in Spain: Identification by nested-PCR and sequencing of a NS5B region","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2015.07.022","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}