{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T13:20:38Z","timestamp":1769347238636,"version":"3.49.0"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Guangxi Science and Technology Bureau","award":["1222003-2-4"],"award-info":[{"award-number":["1222003-2-4"]}]},{"name":"Guangxi Government Senior Scientist Foundation","award":["2011B020"],"award-info":[{"award-number":["2011B020"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2015,11]]},"DOI":"10.1016\/j.jviromet.2015.08.001","type":"journal-article","created":{"date-parts":[[2015,8,8]],"date-time":"2015-08-08T11:49:07Z","timestamp":1439034547000},"page":"9-15","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"special_numbering":"C","title":["Simultaneous detection of eight swine reproductive and respiratory pathogens using a novel GeXP analyser-based multiplex PCR assay"],"prefix":"10.1016","volume":"224","author":[{"given":"Minxiu","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhixun","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liji","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianwen","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiqin","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sisi","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiabo","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaoshan","family":"Pang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mazhar I.","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.jviromet.2015.08.001_bib0005","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.jcv.2009.06.001","article-title":"Development of a real-time RT-PCR for the detection of swine-lineage influenza A (H1N1) virus infections","volume":"45","author":"Carr","year":"2009","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jviromet.2015.08.001_bib0010","first-page":"1050","article-title":"Use of a multiplex RT-PCR assay for simultaneous detection of the North American genotype porcine reproductive and respiratory syndrome virus, swine influenza virus and Japanese encephalitis virus","volume":"9","author":"Chen","year":"2010","journal-title":"Sci. Agric. Sin."},{"issue":"8","key":"10.1016\/j.jviromet.2015.08.001_bib0015","first-page":"6","article-title":"Establishment of SYBR Green I real-time PCR assay for the detection of porcine parvovirus","volume":"47","author":"Chen","year":"2011","journal-title":"Chin. J. Vet. Med."},{"issue":"4","key":"10.1016\/j.jviromet.2015.08.001_bib0020","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1128\/CMR.13.4.559-570.2000","article-title":"Multiplex PCR: optimization and application in diagnostic virology","volume":"13","author":"Elnifro","year":"2000","journal-title":"Clin. Microbiol. Rev."},{"issue":"3","key":"10.1016\/j.jviromet.2015.08.001_bib0025","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1007\/s11259-007-9026-6","article-title":"Development of a novel hot-start multiplex PCR for simultaneous detection of classical swine fever virus African swine fever virus, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus and porcine parvovirus","volume":"32","author":"Giammarioli","year":"2008","journal-title":"Vet. Res. Commun."},{"issue":"1","key":"10.1016\/j.jviromet.2015.08.001_bib0030","doi-asserted-by":"crossref","first-page":"S6","DOI":"10.1097\/01.inf.0000108187.63151.ea","article-title":"Advances in the laboratory diagnosis of viral respiratory disease","volume":"23","author":"Henrickson","year":"2004","journal-title":"Pediatr. Infect. Dis. J."},{"issue":"2","key":"10.1016\/j.jviromet.2015.08.001_bib0035","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1128\/JCM.05828-11","article-title":"Simultaneously typing nine serotypes of enteroviruses associated with hand, foot, and mouth disease by a GeXP analyser-based multiplex reverse transcription-PCR assay","volume":"50","author":"Hu","year":"2012","journal-title":"J. Clin. Microbiol."},{"issue":"2","key":"10.1016\/j.jviromet.2015.08.001_bib0040","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.tvjl.2008.11.016","article-title":"Simultaneous detection of porcine circovirus type 2, classical swine fever virus, porcine parvovirus and porcine reproductive and respiratory syndrome virus in pigs by multiplex polymerase chain reaction","volume":"183","author":"Jiang","year":"2010","journal-title":"Vet. J."},{"key":"10.1016\/j.jviromet.2015.08.001_bib0045","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1186\/1471-2334-12-189","article-title":"The development of a GeXP-based multiplex reverse transcription-PCR assay for simultaneous detection of sixteen human respiratory virus types\/subtypes","volume":"12","author":"Li","year":"2012","journal-title":"BMC Infect. Dis."},{"issue":"1-2","key":"10.1016\/j.jviromet.2015.08.001_bib0050","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.jviromet.2009.07.035","article-title":"A SYBR Green I real-time RT-PCR assay for detection and differentiation of influenza A(H1N1) virus in swine populations","volume":"162","author":"Liu","year":"2009","journal-title":"J. Virol. Methods"},{"issue":"1-2","key":"10.1016\/j.jviromet.2015.08.001_bib0055","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.jviromet.2006.11.018","article-title":"Molecular beacon real-time PCR detection of swine viruses","volume":"140","author":"McKillen","year":"2007","journal-title":"J. Virol. Methods"},{"issue":"1","key":"10.1016\/j.jviromet.2015.08.001_bib0060","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.jviromet.2008.11.019","article-title":"Rapid and sensitive detection of 68 unique varicella zoster virus gene transcripts in five multiplex reverse transcription-polymerase chain reactions","volume":"157","author":"Nagel","year":"2009","journal-title":"J. Virol. Methods"},{"issue":"3","key":"10.1016\/j.jviromet.2015.08.001_bib0065","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1637\/0005-2086(2002)046[0691:DAAOAM]2.0.CO;2","article-title":"Development and application of a multiplex polymerase chain reaction for avian respiratory agents","volume":"46","author":"Pang","year":"2002","journal-title":"Avian Dis."},{"issue":"1\u20132","key":"10.1016\/j.jviromet.2015.08.001_bib0070","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.jviromet.2010.04.031","article-title":"Detection of pandemic influenza A H1N1 virus by multiplex reverse transcription-PCR with a GeXP analyser","volume":"168","author":"Qin","year":"2010","journal-title":"J. Virol. Methods"},{"issue":"5","key":"10.1016\/j.jviromet.2015.08.001_bib0075","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1007\/s00216-008-2436-7","article-title":"Analytical validation of the GeXP analyser and design of a workflow for cancer-biomarker discovery using multiplexed gene-expression profiling","volume":"393","author":"Rai","year":"2009","journal-title":"Anal. Bioanal. Chem."},{"issue":"5","key":"10.1016\/j.jviromet.2015.08.001_bib0080","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1177\/104063870401600501","article-title":"Real-time reverse transcription-polymerase chain reaction assays for the detection and differentiation of North American swine influenza viruses","volume":"16","author":"Richt","year":"2004","journal-title":"J. Vet. Diagn. Invest."},{"key":"10.1016\/j.jviromet.2015.08.001_bib0085","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1128\/JCM.43.1.468-471.2005","article-title":"Diagnostic evaluation of a real-time reverse transcriptase PCR assay for detection of classical swine fever virus","volume":"43","author":"Risatti","year":"2005","journal-title":"J. Clin.Microbiol."},{"issue":"4","key":"10.1016\/j.jviromet.2015.08.001_bib0090","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.diagmicrobio.2010.11.007","article-title":"Comparison of real-time RT-PCR, shell vial culture, and conventional cell culture for the detection of the pandemic influenza A (H1N1) in hospitalized patients","volume":"69","author":"Roa","year":"2011","journal-title":"Diagn. Microbiol. Infect. Dis."},{"issue":"6","key":"10.1016\/j.jviromet.2015.08.001_bib0095","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.4269\/ajtmh.2007.77.1139","article-title":"Detection and isolation of Japanese encephalitis virus from blood clots collected during the acute phase of infection","volume":"77","author":"Sapkal","year":"2007","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"1471-2164","key":"10.1016\/j.jviromet.2015.08.001_bib0100","first-page":"1","article-title":"Temperature switch PCR (TSP): robust assay design for reliable amplification and genotyping of SNPs","volume":"10","author":"Tabone","year":"2009","journal-title":"BMC Genomics"},{"key":"10.1016\/j.jviromet.2015.08.001_bib0105","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2014\/809656","article-title":"Simultaneous detection and differentiation of highly virulent and classical Chinese-type isolation of PRRSV by real-time RT-PCR","volume":"2014","author":"Xiao","year":"2014","journal-title":"J. Immunol. Res."},{"key":"10.1016\/j.jviromet.2015.08.001_bib0110","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.jviromet.2014.07.007","article-title":"Simultaneous typing of nine avian respiratory pathogens using a novel GeXP analyser-based multiplex PCR assay","volume":"207","author":"Xie","year":"2014","journal-title":"J. Virol. Methods"},{"issue":"3","key":"10.1016\/j.jviromet.2015.08.001_bib0115","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.mcp.2006.01.003","article-title":"A multiplex RT-PCR for detection of type A influenza virus and differentiation of avian H5 H7, and H9 hemagglutinin subtypes","volume":"20","author":"Xie","year":"2006","journal-title":"Mol. Cell. Probes"},{"issue":"4","key":"10.1016\/j.jviromet.2015.08.001_bib0120","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1007\/s00436-013-3315-5","article-title":"A duplex quantitative real-time PCR assay for the detection of Haplosporidium and Perkinsus species in shellfish","volume":"112","author":"Xie","year":"2013","journal-title":"Parasitol. Res."},{"issue":"12","key":"10.1016\/j.jviromet.2015.08.001_bib0125","doi-asserted-by":"crossref","first-page":"2245","DOI":"10.1007\/s00705-008-0253-0","article-title":"Development of a real-time multiplex PCR assay for detection of viral pathogens of penaeid shrimp","volume":"153","author":"Xie","year":"2008","journal-title":"Arch. Virol."},{"issue":"6","key":"10.1016\/j.jviromet.2015.08.001_bib0130","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1002\/jmv.23275","article-title":"Genotyping of 11 human papillomaviruses by multiplex PCR with a GeXP analyser","volume":"84","author":"Yang","year":"2012","journal-title":"J. Med. Virol."},{"issue":"3","key":"10.1016\/j.jviromet.2015.08.001_bib0135","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1631\/jzus.2007.B0162","article-title":"Detection of PCV-2 DNA by SYBR Green I-based quantitative PCR","volume":"8","author":"Yang","year":"2007","journal-title":"J. Zhejiang Univ. Sci. B"},{"issue":"5","key":"10.1016\/j.jviromet.2015.08.001_bib0140","first-page":"430","article-title":"Molecular survey of latent pseudorabies virus infection in nervous tissues of slaughtered pigs by nested and real-time PCR","volume":"43","author":"Yoon","year":"2005","journal-title":"J. Microbiol."},{"key":"10.1016\/j.jviromet.2015.08.001_bib0145","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.jviromet.2014.08.001","article-title":"Establishment and application of a multiplex PCR for rapid and simultaneous detection of six viruses in swine","volume":"208","author":"Zeng","year":"2014","journal-title":"J. Virol. Methods"}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093415002669?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093415002669?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T05:21:04Z","timestamp":1567056064000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093415002669"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11]]},"references-count":29,"alternative-id":["S0166093415002669"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2015.08.001","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2015,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Simultaneous detection of eight swine reproductive and respiratory pathogens using a novel GeXP analyser-based multiplex PCR assay","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2015.08.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}