{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:23:24Z","timestamp":1792787004289,"version":"4.5.0"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"International Institute Pasteur Network","id":[{"id":"https:\/\/ror.org\/04cb2ne58","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2017,5]]},"DOI":"10.1016\/j.jviromet.2016.12.019","type":"journal-article","created":{"date-parts":[[2017,2,4]],"date-time":"2017-02-04T20:15:59Z","timestamp":1486239359000},"page":"120-130","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":40,"special_numbering":"C","title":["Development and validation of sensitive real-time RT-PCR assay for broad detection of rabies virus"],"prefix":"10.1016","volume":"243","author":[{"given":"Martin","family":"Faye","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Dacheux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Weidmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie Audrey","family":"Diop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheikh","family":"Loucoubar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Bourhy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amadou Alpha","family":"Sall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ousmane","family":"Faye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2016.12.019_bib0005","article-title":"The cost of canine rabies on four continents","author":"Anderson","year":"2013","journal-title":"Transbound. Emerg. Dis."},{"issue":"June (17)","key":"10.1016\/j.jviromet.2016.12.019_bib0010","article-title":"Heminested reverse-transcriptase polymerase chain reaction (hnRT-PCR) as a tool for rabies virus detection in stored and decomposed samples","volume":"1","author":"Ara\u00fajo","year":"2008","journal-title":"BMC Res Notes"},{"key":"10.1016\/j.jviromet.2016.12.019_bib0015","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0166-0934(02)00062-9","article-title":"A rapid RT-PCR method to differentiate six established genotypes of rabies and rabies-related viruses using TaqMan\u2122 technology","volume":"105","author":"Black","year":"2002","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2016.12.019_bib0020","doi-asserted-by":"crossref","first-page":"2673","DOI":"10.1099\/vir.0.2008\/003913-0","article-title":"The origin and phylogeography of dog rabies virus","volume":"89","author":"Bourhy","year":"2008","journal-title":"J. Gen. Virol."},{"issue":"November","key":"10.1016\/j.jviromet.2016.12.019_bib0025","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.jviromet.2015.08.022","article-title":"Detection of Citrus leprosis virus C using specific primers and TaqMan probe in one-step real-time reverse-transcription polymerase chain reaction assays","volume":"224","author":"Choudhary","year":"2015","journal-title":"J. Virol. Methods"},{"issue":"11","key":"10.1016\/j.jviromet.2016.12.019_bib0030","doi-asserted-by":"crossref","first-page":"3949","DOI":"10.1128\/JCM.01256-10","article-title":"Improved PCR methods for detection of African rabies and rabies-related lyssaviruses","volume":"48","author":"Coertse","year":"2010","journal-title":"J. Clin. Microbiol."},{"issue":"July (7)","key":"10.1016\/j.jviromet.2016.12.019_bib0245","doi-asserted-by":"crossref","first-page":"e0004812","DOI":"10.1371\/journal.pntd.0004812","article-title":"Dual combined real-time reverse transcription polymerase chain reaction assay for the diagnosis of lyssavirus infection","volume":"10","author":"Dacheux","year":"2016","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"December (11)","key":"10.1016\/j.jviromet.2016.12.019_bib0040","doi-asserted-by":"crossref","first-page":"1410","DOI":"10.1086\/592969","article-title":"A reliable diagnosis of human rabies based on analysis of skin biopsy specimens","volume":"47","author":"Dacheux","year":"2008","journal-title":"Clin. Infect. Dis."},{"issue":"11","key":"10.1016\/j.jviromet.2016.12.019_bib0045","doi-asserted-by":"crossref","first-page":"e765","DOI":"10.1371\/journal.pntd.0000765","article-title":"More accurate insight into the incidence of human rabies in developing countries through validated laboratory techniques","volume":"4","author":"Dacheux","year":"2010","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"5","key":"10.1016\/j.jviromet.2016.12.019_bib0050","doi-asserted-by":"crossref","first-page":"1932","DOI":"10.1128\/JCM.02015-10","article-title":"Lyssavirus detection and typing using pyrosequencing","volume":"49","author":"De Benedictis","year":"2011","journal-title":"J. Clin. Microbiol."},{"issue":"April (4)","key":"10.1016\/j.jviromet.2016.12.019_bib0055","doi-asserted-by":"crossref","first-page":"e2057","DOI":"10.1371\/journal.pone.0002057","article-title":"Genomic diversity and evolution of the lyssaviruses","volume":"3","author":"Delmas","year":"2008","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jviromet.2016.12.019_bib0060","doi-asserted-by":"crossref","first-page":"476091","DOI":"10.1155\/2014\/476091","article-title":"Real-time RT-PCR for the detection of lyssavirus species","volume":"2014","author":"Deubelbeiss","year":"2014","journal-title":"J. Vet. Med."},{"key":"10.1016\/j.jviromet.2016.12.019_bib0065","first-page":"45","article-title":"Pathogenesis of rabies","volume":"292","author":"Dietzschold","year":"2005","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"10.1016\/j.jviromet.2016.12.019_bib0070","series-title":"Inter-Laboratory Test for Rabies Diagnosis. Proficiency Test Report Diag-06-2015-V1-EN","author":"European Union Reference Laboratory for Rabies","year":"2015"},{"issue":"August (8)","key":"10.1016\/j.jviromet.2016.12.019_bib0075","first-page":"e28895","article-title":"Development of an in-house TaqMan real time RT-PCR assay to quantify hepatitis C virus RNA in serum and peripheral blood mononuclear cells in patients with chronic hepatitis C virus infection","volume":"15","author":"Saeede Salehi","year":"2015","journal-title":"Hepat. Mon."},{"key":"10.1016\/j.jviromet.2016.12.019_bib0080","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.jviromet.2014.03.014","article-title":"Molecular double-check strategy for the identification and characterization of European Lyssaviruses","volume":"203","author":"Fischer","year":"2014","journal-title":"J. Virol. Methods"},{"issue":"July","key":"10.1016\/j.jviromet.2016.12.019_bib0085","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.jviromet.2014.03.014","article-title":"Molecular double-check strategy for the identification and characterization of European Lyssaviruses","volume":"203","author":"Fischer","year":"2014","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2016.12.019_bib0090","doi-asserted-by":"crossref","first-page":"e530","DOI":"10.1371\/journal.pntd.0000530","article-title":"Emerging technologies for the detection of rabies virus: challenges and hopes in the 21st century","volume":"3","author":"Fooks","year":"2009","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"June (2)","key":"10.1016\/j.jviromet.2016.12.019_bib0095","doi-asserted-by":"crossref","first-page":"141","DOI":"10.11613\/BM.2015.015","article-title":"Understanding Bland Altman analysis","volume":"25","author":"Giavarina","year":"2015","journal-title":"Biochem. Med. (Zagreb)"},{"issue":"2","key":"10.1016\/j.jviromet.2016.12.019_bib0100","first-page":"627","article-title":"ROC curve analysis for medical diagnostic test evaluation","volume":"4","author":"Hajian-Tilaki","year":"2013","journal-title":"Caspian J. Intern. Med."},{"issue":"4","key":"10.1016\/j.jviromet.2016.12.019_bib0105","doi-asserted-by":"crossref","first-page":"e0003709","DOI":"10.1371\/journal.pntd.0003709","article-title":"Estimating the global burden of endemic canine rabies","volume":"9","author":"Hampson","year":"2015","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"4","key":"10.1016\/j.jviromet.2016.12.019_bib0110","doi-asserted-by":"crossref","first-page":"e1001","DOI":"10.1371\/journal.pntd.0001001","article-title":"Evolutionary history of rabies in Ghana","volume":"5","author":"Hayman","year":"2011","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"1","key":"10.1016\/j.jviromet.2016.12.019_bib0250","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.jviromet.2011.07.002","article-title":"A universal real-time assay for the detection of Lyssaviruses","volume":"177","author":"Hayman","year":"2011","journal-title":"J. Virol. Methods"},{"issue":"11","key":"10.1016\/j.jviromet.2016.12.019_bib0120","doi-asserted-by":"crossref","first-page":"2762","DOI":"10.1128\/JCM.35.11.2762-2766.1997","article-title":"Heminested PCR assay for detection of six genotypes of rabies and rabies-related viruses","volume":"35","author":"Heaton","year":"1997","journal-title":"J. Clin. Microbiol."},{"issue":"11","key":"10.1016\/j.jviromet.2016.12.019_bib0125","doi-asserted-by":"crossref","first-page":"3970","DOI":"10.1128\/JCM.00612-10","article-title":"Improved safety for molecular diagnosis of classical rabies viruses by use of a TaqMan real-time reverse transcription-PCR \u201cdouble check\u201d strategy","volume":"48","author":"Hoffmann","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2016.12.019_bib0130","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1086\/344905","article-title":"Management of rabies in humans","volume":"36","author":"Jackson","year":"2003","journal-title":"Clin. Infect. Dis."},{"issue":"June (2)","key":"10.1016\/j.jviromet.2016.12.019_bib0135","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1006\/viro.1995.1285","article-title":"Genetic polymorphism in the rabies virus nucleoprotein gene","volume":"209","author":"Kissi B1","year":"1995","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2016.12.019_bib0140","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1093\/bib\/bbn017","article-title":"MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences","volume":"9","author":"Kumar","year":"2008","journal-title":"Brief. Bioinform."},{"key":"10.1016\/j.jviromet.2016.12.019_bib0145","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1023\/A:1006862304381","article-title":"Intrinsic secondary structure of human TNFR-I mRNA influences the determination of gene expression by RTPCR","volume":"177","author":"Kuo","year":"1997","journal-title":"Mol. Cell. Biochem."},{"key":"10.1016\/j.jviromet.2016.12.019_bib0150","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.jviromet.2014.06.024","article-title":"Effects of carcase decomposition on rabies virus infectivity and detection","volume":"207","author":"McElhinney","year":"2014","journal-title":"J. Virol. Methods"},{"issue":"January (1)","key":"10.1016\/j.jviromet.2016.12.019_bib0155","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.virusres.2011.09.010","article-title":"Molecular epidemiology and a loop-mediated isothermal amplification method for diagnosis of infection with rabies virus in Zambia","volume":"163","author":"Muleya","year":"2012","journal-title":"Virus Res."},{"key":"10.1016\/j.jviromet.2016.12.019_bib0160","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1021\/bi00245a001","article-title":"Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase","volume":"30","author":"Myers","year":"1991","journal-title":"Biochemistry"},{"key":"10.1016\/j.jviromet.2016.12.019_bib0165","series-title":"Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2016","article-title":"Rabies","author":"OIE","year":"2013"},{"key":"10.1016\/j.jviromet.2016.12.019_bib0170","series-title":"Terrestrial Manual 2013. Chapter 1.1.5. Principles and Methods of Validation of Diagnostic Assays for Infectious Diseases","author":"OIE","year":"2013"},{"issue":"8","key":"10.1016\/j.jviromet.2016.12.019_bib0175","doi-asserted-by":"crossref","first-page":"2960","DOI":"10.1128\/JCM.00728-10","article-title":"Comparative analysis of rabies virus reverse transcription-PCR and virus isolation using samples from a patient infected with rabies virus","volume":"48","author":"Panning","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2016.12.019_bib0180","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1099\/0022-1317-71-5-1153","article-title":"Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: theoretical assignment of functional domains","volume":"71","author":"Poch","year":"1990","journal-title":"J. Gen. Virol."},{"issue":"May","key":"10.1016\/j.jviromet.2016.12.019_bib0185","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Hyg."},{"issue":"October (7)","key":"10.1016\/j.jviromet.2016.12.019_bib0190","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1016\/j.ijmm.2015.08.013","article-title":"Instructive even after a decade: complete results of initial virological diagnostics and re-evaluation of molecular data in the German rabies virus \u201coutbreak\u201d caused by transplantations","volume":"305","author":"Ross","year":"2015","journal-title":"Int. J. Med. Microbiol."},{"key":"10.1016\/j.jviromet.2016.12.019_bib0195","doi-asserted-by":"crossref","first-page":"256175","DOI":"10.1155\/2014\/256175","article-title":"A two-step lyssavirus real-time polymerase chain reaction using degenerate primers with superior sensitivity to the fluorescent antigen test","volume":"2014","author":"Suin","year":"2014","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.jviromet.2016.12.019_bib0200","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1126\/science.3287615","article-title":"Measuring the accuracy of diagnostic systems","volume":"240","author":"Swets","year":"1988","journal-title":"Science"},{"issue":"April (Pt 4)","key":"10.1016\/j.jviromet.2016.12.019_bib0205","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1099\/vir.0.007765-0","article-title":"Evolutionary history and dynamics of dog rabies virus in western and central Africa","volume":"90","author":"Talbi","year":"2009","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2016.12.019_bib0210","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1006041","article-title":"Large scale phylogenetic analysis reveals the complex evolutionary trajectories of rabies virus among different carnivore hosts","author":"Troupin","year":"2016","journal-title":"PLoS Pathog."},{"issue":"July (4)","key":"10.1016\/j.jviromet.2016.12.019_bib0215","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1001\/jama.2013.7986","article-title":"Raccoon rabies virus variant transmission through solid organ transplantation","volume":"310","author":"Vora","year":"2013","journal-title":"JAMA"},{"issue":"June (3)","key":"10.1016\/j.jviromet.2016.12.019_bib0220","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1111\/tid.12393","article-title":"Clinical management and humoral immune responses to rabies post-exposure prophylaxis among three patients who received solid organs from a donor with rabies","volume":"17","author":"Vora","year":"2015","journal-title":"Transpl. Infect. Dis."},{"issue":"2","key":"10.1016\/j.jviromet.2016.12.019_bib0225","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.jviromet.2011.05.007","article-title":"Comparative detection of rabies RNA by NASBA, real-time PCR and conventional PCR","volume":"175","author":"Wacharapluesadee","year":"2011","journal-title":"J. Virol. Methods"},{"issue":"September (1\u20132)","key":"10.1016\/j.jviromet.2016.12.019_bib0230","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jviromet.2012.05.013","article-title":"Detection of rabies viral RNA by TaqMan real-time RT-PCR using non-neural specimens from dogs infected with rabies virus","volume":"184","author":"Wacharapluesadee","year":"2012","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2016.12.019_bib0235","series-title":"Expert Consultation on Rabies\u2014Second report","author":"WHO","year":"2013"},{"key":"10.1016\/j.jviromet.2016.12.019_bib0240","series-title":"Expert Consultation on Rabies. Second report","first-page":"1","author":"World Health Organisation","year":"2013"}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093416305584?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093416305584?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T17:05:16Z","timestamp":1601571916000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093416305584"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5]]},"references-count":48,"alternative-id":["S0166093416305584"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2016.12.019","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2017,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and validation of sensitive real-time RT-PCR assay for broad detection of rabies virus","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2016.12.019","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}