{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T22:04:34Z","timestamp":1785881074382,"version":"3.56.0"},"reference-count":19,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Sichuan province science and technology in China","award":["16ZC2686"],"award-info":[{"award-number":["16ZC2686"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2017,10]]},"DOI":"10.1016\/j.jviromet.2017.06.015","type":"journal-article","created":{"date-parts":[[2017,6,30]],"date-time":"2017-06-30T18:15:37Z","timestamp":1498846537000},"page":"166-171","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":31,"special_numbering":"C","title":["Development and application of a reverse transcriptase droplet digital PCR (RT-ddPCR) for sensitive and rapid detection of Japanese encephalitis virus"],"prefix":"10.1016","volume":"248","author":[{"given":"Xulong","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijie","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueping","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zexiao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2017.06.015_bib0005","doi-asserted-by":"crossref","first-page":"e0135767","DOI":"10.1371\/journal.pone.0135767","article-title":"Japanese encephalitis in Assam, India: need to increase healthcare workers' understanding to improve health care","volume":"10","author":"Ahmad","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.jviromet.2017.06.015_bib0010","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1002\/cncr.29976","article-title":"Detection of human papillomavirus type 16 in oropharyngeal squamous cell carcinoma using droplet digital polymerase chain reaction","volume":"122","author":"Biron","year":"2016","journal-title":"Cancer"},{"key":"10.1016\/j.jviromet.2017.06.015_bib0015","doi-asserted-by":"crossref","first-page":"727","DOI":"10.4269\/ajtmh.2011.11-0012","article-title":"Pathogenesis of Japanese encephalitis virus infection in a golden hamster model and evaluation of flavivirus cross-protective immunity","volume":"84","author":"Bosco-Lauth","year":"2011","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.jviromet.2017.06.015_bib0020","doi-asserted-by":"crossref","first-page":"e157","DOI":"10.3324\/haematol.2015.135467","article-title":"Droplet digital polymerase chain reaction for DNMT3A and IDH1\/2 mutations to improve early detection of acute myeloid leukemia relapse after allogeneic hematopoietic stem cell transplantation","volume":"101","author":"Brambati","year":"2016","journal-title":"Haematologica"},{"key":"10.1016\/j.jviromet.2017.06.015_bib0025","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s40121-013-0018-2","article-title":"Japanese B encephalitis: an overview of the disease and use of chimerivax-JE as a preventative vaccine","volume":"2","author":"Chin","year":"2013","journal-title":"Infect. Dis. Ther."},{"key":"10.1016\/j.jviromet.2017.06.015_bib0030","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.jviromet.2014.12.006","article-title":"Rapid and simple detection of Japanese encephalitis virus by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick","volume":"213","author":"Deng","year":"2015","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2017.06.015_bib0035","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1007\/s00705-015-2385-3","article-title":"Comparative evaluation of nucleic acid-based assays for detection of Japanese encephalitis virus in swine blood samples","volume":"160","author":"Dhanze","year":"2015","journal-title":"Arch. Virol."},{"key":"10.1016\/j.jviromet.2017.06.015_bib0040","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.bdq.2015.12.003","article-title":"Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms","volume":"7","author":"Gerdes","year":"2016","journal-title":"Biomol. Detect. Quantif."},{"key":"10.1016\/j.jviromet.2017.06.015_bib0045","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.theriogenology.2008.04.018","article-title":"Infectious causes of embryonic and fetal mortality","volume":"70","author":"Givens","year":"2008","journal-title":"Theriogenology"},{"key":"10.1016\/j.jviromet.2017.06.015_bib0050","doi-asserted-by":"crossref","first-page":"8604","DOI":"10.1021\/ac202028g","article-title":"High-throughput droplet digital PCR system for absolute quantitation of DNA copy number","volume":"83","author":"Hindson","year":"2011","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jviromet.2017.06.015_bib0055","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.jviromet.2010.11.009","article-title":"Development and field evaluation of a nested RT-PCR kit for detecting Japanese encephalitis virus in mosquitoes","volume":"171","author":"Jeong","year":"2011","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2017.06.015_bib0060","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/1743-422X-6-115","article-title":"The prM-independent packaging of pseudotyped Japanese encephalitis virus","volume":"6","author":"Lee","year":"2009","journal-title":"Virol. J."},{"key":"10.1016\/j.jviromet.2017.06.015_bib0065","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1021\/ac202578x","article-title":"Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification","volume":"84","author":"Pinheiro","year":"2012","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jviromet.2017.06.015_bib0070","first-page":"184","article-title":"Detection of Japanese encephalitis virus in samples of JE patients by RT-PCR","volume":"14","author":"Sun","year":"2000","journal-title":"Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi"},{"key":"10.1016\/j.jviromet.2017.06.015_bib0075","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1093\/cid\/cit458","article-title":"Case definitions, diagnostic algorithms, and priorities in encephalitis: consensus statement of the international encephalitis consortium","volume":"57","author":"Venkatesan","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.jviromet.2017.06.015_bib0080","doi-asserted-by":"crossref","first-page":"491","DOI":"10.4161\/hv.18925","article-title":"Japanese encephalitis vaccine: need of the hour in endemic states of India","volume":"8","author":"Verma","year":"2012","journal-title":"Hum. Vaccin Immunother."},{"key":"10.1016\/j.jviromet.2017.06.015_bib0085","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.jviromet.2014.07.007","article-title":"Simultaneous typing of nine avian respiratory pathogens using a novel GeXP analyzer-based multiplex PCR assay","volume":"207","author":"Xie","year":"2014","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2017.06.015_bib0090","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/j.ijpara.2014.08.004","article-title":"Comparison of next-generation droplet digital PCR (ddPCR) with quantitative PCR (qPCR) for enumeration of Cryptosporidium oocysts in faecal samples","volume":"44","author":"Yang","year":"2014","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.jviromet.2017.06.015_bib0095","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1177\/1040638715608358","article-title":"Sensitive detection of Porcine circovirus-2 by droplet digital polymerase chain reaction","volume":"27","author":"Zhao","year":"2015","journal-title":"J. Vet. Diagn. Invest."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093417300368?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093417300368?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,5,31]],"date-time":"2021-05-31T19:31:43Z","timestamp":1622489503000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093417300368"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10]]},"references-count":19,"alternative-id":["S0166093417300368"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2017.06.015","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2017,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and application of a reverse transcriptase droplet digital PCR (RT-ddPCR) for sensitive and rapid detection of Japanese encephalitis virus","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2017.06.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}