{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T01:10:25Z","timestamp":1790125825962,"version":"4.0.1"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,10,18]],"date-time":"2017-10-18T00:00:00Z","timestamp":1508284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014588","name":"Sanofi Pasteur","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100014588","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2018,1]]},"DOI":"10.1016\/j.jviromet.2017.10.011","type":"journal-article","created":{"date-parts":[[2017,10,13]],"date-time":"2017-10-13T03:03:18Z","timestamp":1507863798000},"page":"46-53","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["A latex agglutination assay to quantify the amount of hemagglutinin protein in adjuvanted low-dose influenza monovalent vaccines"],"prefix":"10.1016","volume":"251","author":[{"given":"Sophie","family":"Buffin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nabila","family":"Ikhelef","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Prudent","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseline","family":"Dubayle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nolwenn","family":"Nougarede","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Pierre","family":"Varenne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Moste","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Legastelois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2017.10.011_bib0005","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1016\/j.vaccine.2010.11.042","article-title":"Lessons from pandemic influenza A(H1N1): The research-based vaccine industry\u2019s perspective","volume":"29","author":"Abelin","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0010","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1111\/j.1750-2659.2012.00375.x","article-title":"Development of an enzyme-linked immunoassay for the quantitation of influenza haemagglutinin: an alternative method to single radial immunodiffusion","volume":"7","author":"Bodle","year":"2013","journal-title":"Influenza Other Respir. Viruses"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0015","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1016\/S0140-6736(06)68656-X","article-title":"Safety and immunogenicity of an inactivated split-virion influenza A\/Vietnam\/1194\/2004 (H5N1) vaccine: phase I randomised trial","volume":"367","author":"Bresson","year":"2006","journal-title":"Lancet Lond. Engl."},{"key":"10.1016\/j.jviromet.2017.10.011_bib0020","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1093\/clinids\/5.4.737","article-title":"Reactogenicity, immunogenicity, and antibody persistence in adults given inactivated influenza virus vaccines \u2013 1978","volume":"5","author":"Cate","year":"1983","journal-title":"Rev. Infect. Dis."},{"key":"10.1016\/j.jviromet.2017.10.011_bib0025","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1177\/104063870701900203","article-title":"A latex agglutination test for the rapid detection of avian influenza virus subtype H5N1 and its clinical application","volume":"19","author":"Chen","year":"2007","journal-title":"J. Vet. Diagn. Investig. Off. Publ. Am. Assoc. Vet. Lab. Diagn. Inc"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0030","doi-asserted-by":"crossref","first-page":"6068","DOI":"10.1016\/j.vaccine.2008.09.015","article-title":"Universal antibodies and their applications to the quantitative determination of virtually all subtypes of the influenza A viral hemagglutinins","volume":"26","author":"Chun","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0035","doi-asserted-by":"crossref","first-page":"4762","DOI":"10.1016\/j.vaccine.2012.05.036","article-title":"Simultaneous quantification of the viral antigens hemagglutinin and neuraminidase in influenza vaccines by LC-MSE","volume":"30","author":"Creskey","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0040","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1038\/39218","article-title":"A pandemic warning?","volume":"389","author":"de Jong","year":"1997","journal-title":"Nature"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0045","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1126\/science.1204839","article-title":"A highly conserved neutralizing epitope on group 2 influenza A viruses","volume":"333","author":"Ekiert","year":"2011","journal-title":"Science"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0050","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1056\/NEJMoa1304459","article-title":"Human infection with a novel avian-origin influenza A (H7N9) virus","volume":"368","author":"Gao","year":"2013","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.jviromet.2017.10.011_bib0055","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1111\/irv.12001","article-title":"Label-free mass spectrometry-based quantification of hemagglutinin and neuraminidase in influenza virus preparations and vaccines","volume":"7","author":"Getie-Kebtie","year":"2013","journal-title":"Influenza Other Respir. Viruses"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0060","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1378\/chest.12-1711","article-title":"Immunobiology of influenza vaccines","volume":"143","author":"Gomez Lorenzo","year":"2013","journal-title":"Chest"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0065","doi-asserted-by":"crossref","first-page":"e55428","DOI":"10.1371\/journal.pone.0055428","article-title":"A novel synthetic receptor-based immunoassay for influenza vaccine quantification","volume":"8","author":"Hashem","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0070","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1017\/S0022172400022610","article-title":"The role of serum haemagglutination-inhibiting antibody in protection against challenge infection with influenza A2 and B viruses","volume":"70","author":"Hobson","year":"1972","journal-title":"J. Hyg. (Lond.)"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0075","doi-asserted-by":"crossref","first-page":"e103294","DOI":"10.1371\/journal.pone.0103294","article-title":"The breadth of cross sub-type neutralisation activity of a single domain antibody to influenza hemagglutinin can be increased by antibody valency","volume":"9","author":"Hufton","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0080","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s12575-015-0019-5","article-title":"Evaluation of Epic\u00ae label-free technology to quantify functional recombinant hemagglutinin","volume":"17","author":"Jiang","year":"2015","journal-title":"Biol. Proced. Online"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0085","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1586\/erv.11.130","article-title":"Influenza viral neuraminidase: the forgotten antigen","volume":"10","author":"Johansson","year":"2011","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0090","first-page":"431","article-title":"Vaccines for pandemic influenza: summary of recent clinical trials","volume":"333","author":"Keitel","year":"2009","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"10.1016\/j.jviromet.2017.10.011_bib0095","doi-asserted-by":"crossref","first-page":"1918","DOI":"10.1016\/j.vaccine.2017.02.028","article-title":"VaxArray assessment of influenza split vaccine potency and stability","volume":"35","author":"Kuck","year":"2017","journal-title":"Vaccine"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0100","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.jviromet.2011.08.027","article-title":"Avian glycan-specific IgM monoclonal antibodies for the detection and quantitation of type A and B haemagglutinins in egg-derived influenza vaccines","volume":"178","author":"Legastelois","year":"2011","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0105","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1086\/590913","article-title":"An adjuvanted, low-dose, pandemic influenza A (H5N1) vaccine candidate is safe, immunogenic, and induces cross-reactive immune responses in healthy adults","volume":"198","author":"Levie","year":"2008","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jviromet.2017.10.011_bib0110","doi-asserted-by":"crossref","first-page":"3377","DOI":"10.1016\/j.vaccine.2011.02.090","article-title":"Optimization and qualification of a quantitative reversed-phase HPLC method for hemagglutinin in influenza preparations and its comparative evaluation with biochemical assays","volume":"29","author":"Lorbetskie","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0115","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.aca.2008.06.005","article-title":"Monitoring of influenza virus hemagglutinin in process samples using weak affinity ligands and surface plasmon resonance","volume":"623","author":"Mandenius","year":"2008","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0120","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/s00430-009-0118-5","article-title":"Novel swine-origin influenza A virus in humans: another pandemic knocking at the door","volume":"198","author":"Michaelis","year":"2009","journal-title":"Med. Microbiol. Immunol. (Berl.)"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0125","doi-asserted-by":"crossref","first-page":"90","DOI":"10.3390\/vaccines3010090","article-title":"Assaying the potency of influenza vaccines","volume":"3","author":"Minor","year":"2015","journal-title":"Vaccines"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0130","series-title":"NIBSC \u2013 Influenza Research [WWW Document]","author":"NIBSC","year":"2017"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0135","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1038\/nature08157","article-title":"Emergence and pandemic potential of swine-origin H1N1 influenza virus","volume":"459","author":"Neumann","year":"2009","journal-title":"Nature"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0140","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/j.vaccine.2009.10.070","article-title":"A novel assay for influenza virus quantification using surface plasmon resonance","volume":"28","author":"Nilsson","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0145","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.1016\/S0264-410X(00)00448-5","article-title":"Influenza vaccines: a review and rationale for use in developed and underdeveloped countries","volume":"19","author":"Poland","year":"2001","journal-title":"Vaccine"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0150","series-title":"Use of Licensed H5N1 Influenza Vaccines in the Interpandemic Period [WWW Document]","author":"SAGE Working Group","year":"2009"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0155","first-page":"223","article-title":"A single-radial-immunodiffusion technique for the assay of influenza haemagglutinin antigen","volume":"52","author":"Schild","year":"1975","journal-title":"Bull. World Health Organ."},{"key":"10.1016\/j.jviromet.2017.10.011_bib0160","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1038\/nm0909-986","article-title":"Swine flu agitates the adjuvant debate","volume":"15","author":"Schubert","year":"2009","journal-title":"Nat. Med."},{"key":"10.1016\/j.jviromet.2017.10.011_bib0165","unstructured":"Sizer, P.J., King-Haughey, J., Simpkin, D., Williams, R., 2014. Assays for adsorbed influenza vaccines. Patent US8685654 B2."},{"key":"10.1016\/j.jviromet.2017.10.011_bib0170","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1586\/erv.09.5","article-title":"Emulsion-based adjuvants for influenza vaccines","volume":"8","author":"Vogel","year":"2009","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0175","series-title":"Generic Protocol for the Calibration of Seasonal and Pandemic Influenza Antigen Working Reagents by WHO Essential Regulatory Laboratories. [WWW Document]","author":"WHO","year":"2012"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0180","series-title":"WHO. Influenza (Seasonal) [WWW Document]","author":"WHO","year":"2016"},{"key":"10.1016\/j.jviromet.2017.10.011_bib0185","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/0378-1135(93)90027-5","article-title":"Single-radial-immunodiffusion as an in vitro potency assay for human inactivated viral vaccines","volume":"37","author":"Williams","year":"1993","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.jviromet.2017.10.011_bib0190","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0092-1157(77)80008-5","article-title":"An improved single-radial-immunodiffusion technique for the assay of influenza haemagglutinin antigen: application for potency determinations of inactivated whole virus and subunit vaccines","volume":"5","author":"Wood","year":"1977","journal-title":"J. Biol. Stand."},{"key":"10.1016\/j.jviromet.2017.10.011_bib0195","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1093\/clinids\/5.4.758","article-title":"Antigenicity and reactogenicity of influenza A\/USSR\/77 virus vaccine in children\u2013a multicentered evaluation of dosage and safety","volume":"5","author":"Wright","year":"1983","journal-title":"Rev. Infect. Dis."},{"issue":"Suppl. B","key":"10.1016\/j.jviromet.2017.10.011_bib0200","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/jac\/44.suppl_2.3","article-title":"Epidemiology and pathogenesis of influenza","volume":"44","author":"Zambon","year":"1999","journal-title":"J. Antimicrob. Chemother."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093417304445?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093417304445?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T06:10:56Z","timestamp":1589782256000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093417304445"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1]]},"references-count":40,"alternative-id":["S0166093417304445"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2017.10.011","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2018,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A latex agglutination assay to quantify the amount of hemagglutinin protein in adjuvanted low-dose influenza monovalent vaccines","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2017.10.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}