{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T10:28:43Z","timestamp":1784543323883,"version":"3.55.0"},"reference-count":23,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81601773"],"award-info":[{"award-number":["81601773"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Taian Municipal Science & Technology Development Program","award":["2017NS0107"],"award-info":[{"award-number":["2017NS0107"]}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong province","doi-asserted-by":"crossref","award":["ZR2014JL051"],"award-info":[{"award-number":["ZR2014JL051"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Taishan Scholars program of Shandong Province","award":["ts201511056"],"award-info":[{"award-number":["ts201511056"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2018,8]]},"DOI":"10.1016\/j.jviromet.2018.05.005","type":"journal-article","created":{"date-parts":[[2018,5,25]],"date-time":"2018-05-25T22:30:02Z","timestamp":1527287402000},"page":"7-12","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"special_numbering":"C","title":["Rapid detection of hand, foot and mouth disease enterovirus genotypes by multiplex PCR"],"prefix":"10.1016","volume":"258","author":[{"given":"Min","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenjie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lehai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Carr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weifeng","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.jviromet.2018.05.005_bib0005","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.jcv.2010.02.002","article-title":"Co-circulation of coxsackieviruses A6 and A10 in hand, foot and mouth disease outbreak in Finland","volume":"48","author":"Blomqvist","year":"2010","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jviromet.2018.05.005_bib0010","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.mcp.2017.02.003","article-title":"Rapid detection of Enterovirus and Coxsackievirus A10 by a TaqMan based duplex one-step real time RT-PCR assay","volume":"33","author":"Chen","year":"2017","journal-title":"Mol. Cell. Probes."},{"issue":"6","key":"10.1016\/j.jviromet.2018.05.005_bib0015","first-page":"624","article-title":"Molecular epidemiology of HFMD-associated pathogen coxsackievirus A6 in Fujian Province, 2011-2013","volume":"30","author":"Chen","year":"2014","journal-title":"Bing Du Xue Bao"},{"key":"10.1016\/j.jviromet.2018.05.005_bib0020","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s40249-016-0124-y","article-title":"Serum cholinesterase: a potential assistant biomarker for hand, foot, and mouth disease caused by enterovirus 71 infection","volume":"5","author":"Cheng","year":"2016","journal-title":"Infect. Dis. Poverty"},{"issue":"4","key":"10.1016\/j.jviromet.2018.05.005_bib0025","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0061451","article-title":"The development and application of the two real-time RT-PCR assays to detect the pathogen of HFMD","volume":"8","author":"Cui","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.jviromet.2018.05.005_bib0030","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.meegid.2017.05.024","article-title":"Genetic characterization of enterovirus strains identified in Hand, Foot and Mouth Disease (HFMD): emergence of B1c, C1 subgenotypes, E2 sublineage of CVA16, EV71 and CVA6 strains in India","volume":"54","author":"Ganorkar","year":"2017","journal-title":"Infect. Genet. Evol."},{"issue":"2","key":"10.1016\/j.jviromet.2018.05.005_bib0035","first-page":"178","article-title":"Development and application of a duplex real-time RT-PCR assay for simultaneous detection of Coxsackievirus A6 and Coxsackievirus A10","volume":"47","author":"He","year":"2017","journal-title":"Ann. Clin. Lab. Sci."},{"issue":"7","key":"10.1016\/j.jviromet.2018.05.005_bib0040","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1002\/jmv.23588","article-title":"Development of duplex real-time RT-PCR based on Taqman technology for detecting simultaneously the genome of pan-enterovirus and enterovirus 71","volume":"85","author":"Hwang","year":"2013","journal-title":"J. Med. Virol"},{"issue":"3","key":"10.1016\/j.jviromet.2018.05.005_bib0045","first-page":"224","article-title":"Development of EV71, CA16 and other enterovirus vrial real-time qualitative PCR diagnostic kit","volume":"27","author":"Li","year":"2013","journal-title":"Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi"},{"issue":"5","key":"10.1016\/j.jviromet.2018.05.005_bib0050","doi-asserted-by":"crossref","first-page":"E110","DOI":"10.1111\/j.1469-0691.2012.03789.x","article-title":"Outbreak of hand, foot and mouth disease\/herpangina associated with coxsackievirus A6 and A10 infections in 2010, France: a large citywide, prospective observational study","volume":"18","author":"Mirand","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"issue":"3","key":"10.1016\/j.jviromet.2018.05.005_bib0055","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.cels.2016.08.013","article-title":"Multi-omics quantification of species variation of escherichia coli links molecular features with strain phenotypes","volume":"28","author":"Monk","year":"2016","journal-title":"Cell. Syst."},{"issue":"4","key":"10.1016\/j.jviromet.2018.05.005_bib0060","doi-asserted-by":"crossref","first-page":"17496","DOI":"10.4238\/2015.December.21.21","article-title":"A real-time RT-PCR assay for rapid detection of coxsackievirus A10","volume":"14","author":"Mu","year":"2015","journal-title":"Genet. Mol. Res."},{"key":"10.1016\/j.jviromet.2018.05.005_bib0065","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.jviromet.2017.06.013","article-title":"Development of single-step multiplex real-time RT-PCR assays for rapid diagnosis of enterovirus 71, coxsackievirus A6, and A16 in patients with hand, foot, and mouth disease","volume":"248","author":"Puenpa","year":"2017","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2018.05.005_bib0070","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.meegid.2017.06.004","article-title":"Synonymous codon usage analysis of hand, foot and mouth disease viruses: a comparative study on coxsackievirus A6, A10, A16, and enterovirus 71 from 2008 to 2015","volume":"53","author":"Su","year":"2017","journal-title":"Infect. Genet. Evol."},{"issue":"3","key":"10.1016\/j.jviromet.2018.05.005_bib0075","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.diagmicrobio.2008.02.009","article-title":"Development of multiplex real-time hybridization probe reverse transcriptase polymerase chain reaction for specific detection and differentiation of Enterovirus 71 and Coxsackievirus A16","volume":"61","author":"Tan","year":"2008","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.jviromet.2018.05.005_bib0080","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1186\/s12985-015-0316-2","article-title":"Validation and utilization of an internally controlled multiplex real-time RT-PCR assay for simultaneous detection of enteroviruses and enterovirus A71 associated with hand foot and mouth disease","volume":"12","author":"Thanh","year":"2015","journal-title":"Virol. J."},{"key":"10.1016\/j.jviromet.2018.05.005_bib0085","doi-asserted-by":"crossref","first-page":"2508","DOI":"10.12659\/MSM.901364","article-title":"Serotyping and genetic characterization of Hand, Foot, and Mouth Disease (HFMD)-associated enteroviruses of No-EV71 and Non-CVA16 circulating in Fujian, China, 2011-2015","volume":"23","author":"Weng","year":"2017","journal-title":"Med. Sci. Monit."},{"issue":"1","key":"10.1016\/j.jviromet.2018.05.005_bib0090","first-page":"98","article-title":"Simultaneous detection of enterovirus 71 and coxsackievirus A16 by multiplex real-time PCR with an internal control","volume":"49","author":"Xiao","year":"2009","journal-title":"Wei Sheng Wu Xue Bao"},{"key":"10.1016\/j.jviromet.2018.05.005_bib0095","doi-asserted-by":"crossref","first-page":"10550","DOI":"10.1038\/srep10550","article-title":"The epidemiological study of Coxsackievirus A6 revealing hand, foot and mouth disease epidemic patterns in Guangdong, China","volume":"5","author":"Zeng","year":"2015","journal-title":"Sci. Rep."},{"issue":"9","key":"10.1016\/j.jviromet.2018.05.005_bib0100","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.02450-16","article-title":"A Neonatal Murine Model of Coxsackievirus A6 infection for evaluation of antiviral and vaccine efficacy","volume":"91","author":"Zhang","year":"2017","journal-title":"J. Virol."},{"issue":"13","key":"10.1016\/j.jviromet.2018.05.005_bib0105","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00333-17","article-title":"Protective efficacies of formaldehyde-inactivated whole-virus vaccine and antivirals in a Murine Model of Coxsackievirus A10 infection","volume":"91","author":"Zhang","year":"2017","journal-title":"J. Virol."},{"issue":"2","key":"10.1016\/j.jviromet.2018.05.005_bib0110","first-page":"127","article-title":"Etiology of hand, foot and mouth disease in Guangzhou in 2008","volume":"48","author":"Zhu","year":"2010","journal-title":"Zhonghua Er Ke Za Zhi"},{"issue":"6","key":"10.1016\/j.jviromet.2018.05.005_bib0115","first-page":"1645","article-title":"Preparation of SLA-1-632-TPK gene and construction of recombinant plasmid SLA-1-TPK \/ pMD19-T in Topek pig","volume":"44","author":"Zhu","year":"2017","journal-title":"Chin. J. Animal Husb. Vet. Med."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093418300508?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093418300508?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,12,5]],"date-time":"2019-12-05T01:17:48Z","timestamp":1575508668000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093418300508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8]]},"references-count":23,"alternative-id":["S0166093418300508"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2018.05.005","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2018,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Rapid detection of hand, foot and mouth disease enterovirus genotypes by multiplex PCR","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2018.05.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}