{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T06:51:01Z","timestamp":1765608661401,"version":"3.44.0"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Roche Diagnostics"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2019,7]]},"DOI":"10.1016\/j.jviromet.2019.03.015","type":"journal-article","created":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T11:03:05Z","timestamp":1554116585000},"page":"49-54","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":32,"special_numbering":"C","title":["Clinical evaluation of multiplex RT-PCR assays for the detection of influenza A\/B and respiratory syncytial virus using a high throughput system"],"prefix":"10.1016","volume":"269","author":[{"given":"Ulrich","family":"Eigner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svenja","family":"Reucher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadine","family":"Hefner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandrine","family":"Staffa-Peichl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa","family":"Kolb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrike","family":"Betz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Holfelder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gene","family":"Spier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Pfefferle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9468-7944","authenticated-orcid":false,"given":"Marc","family":"L\u00fctgehetmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2019.03.015_bib0005","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00930-18","article-title":"Comparison of six sample-to-Answer influenza A\/B and respiratory syncytial virus nucleic acid amplification assays using respiratory specimens from children","volume":"56","author":"Banerjee","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0010","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1111\/trf.14460","article-title":"Zika virus RNA polymerase chain reaction on the utility channel of a commercial nucleic acid testing system","volume":"58","author":"Boujnan","year":"2018","journal-title":"Transfusion"},{"key":"10.1016\/j.jviromet.2019.03.015_bib0015","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s40266-015-0258-9","article-title":"Respiratory syncytial virus infection in older adults: an under-recognized problem","volume":"32","author":"Branche","year":"2015","journal-title":"Drugs Aging"},{"year":"2018","series-title":"Estimated Influenza Illnesses, Medical Visits, Hospitalizations, and Deaths in the United States \u2014 2017\u20132018 Influenza Season","author":"CDC","key":"10.1016\/j.jviromet.2019.03.015_bib0020"},{"year":"2018","series-title":"Influenza (Flu) Viruses","author":"CDC","key":"10.1016\/j.jviromet.2019.03.015_bib0025"},{"year":"2018","series-title":"Information for Clinicians on Influenza Virus Testing","author":"CDC","key":"10.1016\/j.jviromet.2019.03.015_bib0030"},{"key":"10.1016\/j.jviromet.2019.03.015_bib0035","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.diagmicrobio.2017.11.010","article-title":"Evaluation of the molecular Xpert Xpress Flu\/RSV assay vs. Alere i Influenza A & B assay for rapid detection of influenza viruses","volume":"90","author":"Chen","year":"2018","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0040","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1128\/CMR.05012-11","article-title":"Two years after pandemic influenza A\/2009\/H1N1: what have we learned?","volume":"25","author":"Cheng","year":"2012","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0045","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1080\/14737159.2017.1275962","article-title":"The cobas(R) 6800\/8800 System: a new era of automation in molecular diagnostics","volume":"17","author":"Cobb","year":"2017","journal-title":"Expert Rev. Mol. Diagn."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0050","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1186\/1471-2105-13-101","article-title":"MELTING, a flexible platform to predict the melting temperatures of nucleic acids","volume":"13","author":"Dumousseau","year":"2012","journal-title":"BMC Bioinf."},{"year":"2018","series-title":"Seasonal Influenza - Annual Epidemiological Report for 2017 - 2018","author":"ECDC","key":"10.1016\/j.jviromet.2019.03.015_bib0055"},{"key":"10.1016\/j.jviromet.2019.03.015_bib0060","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1111\/j.1532-5415.2008.01775.x","article-title":"Long-term care facilities: a cornucopia of viral pathogens","volume":"56","author":"Falsey","year":"2008","journal-title":"J. Am. Geriatr. Soc."},{"year":"2014","series-title":"510(k) summary","author":"FDA","key":"10.1016\/j.jviromet.2019.03.015_bib0065"},{"key":"10.1016\/j.jviromet.2019.03.015_bib0070","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1002\/cncr.29833","article-title":"Acute respiratory infections in children and adolescents with acute lymphoblastic leukemia","volume":"122","author":"Hakim","year":"2016","journal-title":"Cancer"},{"key":"10.1016\/j.jviromet.2019.03.015_bib0075","doi-asserted-by":"crossref","first-page":"2431","DOI":"10.1128\/JCM.00318-17","article-title":"Multicenter clinical evaluation of the luminex Aries flu A\/B & RSV assay for pediatric and adult respiratory tract specimens","volume":"55","author":"Juretschko","year":"2017","journal-title":"J. Clin. Microbiol."},{"year":"2017","series-title":"Update: Increase in Human Infections With Novel Asian Lineage Avian Influenza a(H7N9) Viruses During the Fifth Epidemic - China, October 1, 2016-August 7, 2017. MMWR. Morb Mortal Wkly Rep 66, 928-932","author":"Kile","key":"10.1016\/j.jviromet.2019.03.015_bib0080"},{"key":"10.1016\/j.jviromet.2019.03.015_bib0085","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01691-17","article-title":"Parallel validation of three molecular devices for simultaneous detection and identification of influenza a and B and respiratory syncytial viruses","volume":"56","author":"Ling","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0090","doi-asserted-by":"crossref","DOI":"10.1177\/1756283X17722745","article-title":"Commutability and concordance of four hepatitis B virus DNA assays in an international multicenter study","author":"Maasoumy","year":"2017","journal-title":"Therap. Adv. Gastroenterol."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0095","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.vaccine.2017.11.018","article-title":"The risk of lower respiratory tract infection following influenza virus infection: a systematic and narrative review","volume":"36","author":"Malosh","year":"2018","journal-title":"Vaccine"},{"key":"10.1016\/j.jviromet.2019.03.015_bib0100","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1016\/j.bbmt.2005.07.007","article-title":"Prospective study of the incidence, clinical features, and outcome of symptomatic upper and lower respiratory tract infections by respiratory viruses in adult recipients of hematopoietic stem cell transplants for hematologic malignancies","volume":"11","author":"Martino","year":"2005","journal-title":"Biol. Blood Marrow Transplant."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0105","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/j.1750-2659.2009.00089.x","article-title":"Understanding influenza transmission, immunity and pandemic threats","volume":"3","author":"Mathews","year":"2009","journal-title":"Influenza Other Respir. Viruses"},{"key":"10.1016\/j.jviromet.2019.03.015_bib0110","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.jcv.2017.08.018","article-title":"The performance of Luminex ARIES\u00ae Flu A\/B & RSV and Cepheid Xpert\u00ae Flu\/RSV XC for the detection of influenza A, influenza B, and respiratory syncytial virus in prospective patient samples","volume":"95","author":"McMullen","year":"2017","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0115","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1016\/S0140-6736(10)60206-1","article-title":"Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta-analysis","volume":"375","author":"Nair","year":"2010","journal-title":"Lancet"},{"key":"10.1016\/j.jviromet.2019.03.015_bib0120","doi-asserted-by":"crossref","first-page":"2096","DOI":"10.1128\/JCM.00549-16","article-title":"Impact of early detection of respiratory viruses by multiplex PCR assay on clinical outcomes in adult patients","volume":"54","author":"Rappo","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0125","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.2146\/ajhp080438","article-title":"Impact of respiratory syncytial virus in the United States","volume":"65","author":"Robinson","year":"2008","journal-title":"Am. J. Health System Pharm."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0130","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.jmoldx.2017.01.009","article-title":"Impact of rapid molecular respiratory virus testing on real-time decision making in a pediatric emergency department","volume":"19","author":"Rogan","year":"2017","journal-title":"J. Mol. Diagn."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0135","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1146\/annurev.biophys.32.110601.141800","article-title":"The thermodynamics of DNA structural motifs","volume":"33","author":"SantaLucia","year":"2004","journal-title":"Annu. Rev. Biophys. Biomol. Struct."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0140","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1097\/INF.0b013e318284b146","article-title":"Role of a respiratory viral panel in the clinical management of pediatric inpatients","volume":"32","author":"Schulert","year":"2013","journal-title":"Pediatr. Infect. Dis. J."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0145","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1016\/S0140-6736(17)30938-8","article-title":"Global, regional, and national disease burden estimates of acute lower respiratory infections due to respiratory syncytial virus in young children in 2015: a systematic review and modelling study","volume":"390","author":"Shi","year":"2017","journal-title":"Lancet"},{"key":"10.1016\/j.jviromet.2019.03.015_bib0150","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2017.22.13.30494","article-title":"GISAID: global initiative on sharing all influenza data - from vision to reality","volume":"22","author":"Shu","year":"2017","journal-title":"Euro Surveill."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0155","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01996-18","article-title":"Evaluation of the Performance of the cobas\u00ae CT\/NG Test for Use on the cobas\u00ae 6800\/8800 Systems for the Detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) in Male and Female Urogenital Samples","author":"Van Der Pol","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0160","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1128\/JCM.02193-16","article-title":"Multicenter comparison study of both analytical and clinical performance across four Roche hepatitis C virus RNA assays utilizing different platforms","volume":"55","author":"Vermehren","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0165","doi-asserted-by":"crossref","first-page":"1731","DOI":"10.1093\/cid\/cit639","article-title":"Respiratory syncytial virus lower respiratory disease in hematopoietic cell transplant recipients: viral RNA detection in blood, antiviral treatment, and clinical outcomes","volume":"57","author":"Waghmare","year":"2013","journal-title":"Clin. Infect. Dis."},{"year":"2018","series-title":"Global Influenza Surveillance and Response System (GISRS)","author":"WHO","key":"10.1016\/j.jviromet.2019.03.015_bib0170"},{"year":"2018","series-title":"Influenza Fact Sheet","author":"WHO","key":"10.1016\/j.jviromet.2019.03.015_bib0175"},{"year":"2018","series-title":"The Top 10 Causes of Death","author":"WHO","key":"10.1016\/j.jviromet.2019.03.015_bib0180"},{"key":"10.1016\/j.jviromet.2019.03.015_bib0185","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1309\/AJCPH7X3NLYZPHBW","article-title":"Implementation of filmarray respiratory viral panel in a core laboratory improves testing turnaround time and patient care","volume":"139","author":"Xu","year":"2013","journal-title":"Am. J. Clin. Pathol."},{"key":"10.1016\/j.jviromet.2019.03.015_bib0190","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.jcv.2018.02.017","article-title":"Diagnosis and monitoring of HCV infection using the cobas\u00ae HCV test for use on the cobas\u00ae6800\/8800 systems","volume":"102","author":"Yao","year":"2018","journal-title":"J. Clin. Virol."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093418306311?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093418306311?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T10:51:08Z","timestamp":1759056668000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093418306311"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7]]},"references-count":38,"alternative-id":["S0166093418306311"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2019.03.015","relation":{},"ISSN":["0166-0934"],"issn-type":[{"type":"print","value":"0166-0934"}],"subject":[],"published":{"date-parts":[[2019,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Clinical evaluation of multiplex RT-PCR assays for the detection of influenza A\/B and respiratory syncytial virus using a high throughput system","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2019.03.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}