{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:52:59Z","timestamp":1761897179561,"version":"3.44.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2020,2]]},"DOI":"10.1016\/j.jviromet.2019.113796","type":"journal-article","created":{"date-parts":[[2019,12,5]],"date-time":"2019-12-05T13:01:05Z","timestamp":1575550865000},"page":"113796","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Generation and molecular docking analysis of specific single-chain variable fragments selected by phage display against the recombinant nucleocapsid protein of fig mosaic virus"],"prefix":"10.1016","volume":"276","author":[{"given":"Morteza","family":"Shahmirzaie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Reza","family":"Safarnejad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farshad","family":"Rakhshandehroo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossein","family":"Safarpour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farshad H.","family":"Shirazi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamid Reza","family":"Zamanizadeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toufic","family":"Elbeaino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"7","key":"10.1016\/j.jviromet.2019.113796_bib0005","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1021\/bi9517244","article-title":"Recombinant Phabs reactive with 7, 8-dihydro-8-oxoguanine, a major oxidative DNA lesion","volume":"35","author":"Bespalov","year":"1996","journal-title":"Biochemistry"},{"key":"10.1016\/j.jviromet.2019.113796_bib0010","first-page":"893","article-title":"Fig mosaic","volume":"51","author":"Blodgett","year":"1967","journal-title":"Plant Dis. Rep."},{"issue":"11","key":"10.1016\/j.jviromet.2019.113796_bib0015","first-page":"887","article-title":"A mosaic of the fig in California","volume":"23","author":"Condit","year":"1933","journal-title":"Phytopathol."},{"issue":"4","key":"10.1016\/j.jviromet.2019.113796_bib0020","first-page":"519","article-title":"Genetic diversity of male fig (Ficus carica caprificus L.) genotypes with random amplified polymorphic DNA (RAPD) markers","volume":"10","author":"Dalk\u0131l\u0131\u00e7","year":"2011","journal-title":"Afr. J. Biotechnol."},{"issue":"2","key":"10.1016\/j.jviromet.2019.113796_bib0025","first-page":"187","article-title":"Partial characterization of a closterovirus associated with a chlorotic mottling of fig","volume":"88","author":"Elbeaino","year":"2006","journal-title":"J. Plant Pathol."},{"issue":"1","key":"10.1016\/j.jviromet.2019.113796_bib0030","first-page":"119","article-title":"Identification of a second member of the family Closteroviridae in mosaic-diseased figs","volume":"89","author":"Elbeaino","year":"2007","journal-title":"J. Plant Pathol."},{"issue":"11","key":"10.1016\/j.jviromet.2019.113796_bib0035","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1007\/s00705-009-0509-3","article-title":"Complete nucleotides sequence of four viral RNAs segments of fig mosaic virus","volume":"154","author":"Elbeaino","year":"2009","journal-title":"Arch. Virol."},{"issue":"5","key":"10.1016\/j.jviromet.2019.113796_bib0040","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1099\/vir.0.008649-0","article-title":"A multipartite negative-sense single-stranded RNA virus is the putative agent of fig mosaic disease","volume":"90","author":"Elbeaino","year":"2009","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2019.113796_bib0045","first-page":"165","article-title":"Fig mild mottle-associated virus, a novel closterovirus infecting fig","author":"Elbeaino","year":"2010","journal-title":"J. Plant Pathol."},{"key":"10.1016\/j.jviromet.2019.113796_bib0050","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.virusres.2011.07.022","article-title":"The complete sequence of Fig fleck-associated virus, a novel Tymoviridae member infecting fig","volume":"161","author":"Elbeaino","year":"2011","journal-title":"Virus Res."},{"issue":"3","key":"10.1016\/j.jviromet.2019.113796_bib0055","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1007\/s11262-011-0581-0","article-title":"The complete nucleotide sequence and genome organization of fig cryptic virus, a novel bipartite dsRNA virus infecting fig, widely distributed in the Mediterranean basin","volume":"42","author":"Elbeaino","year":"2011","journal-title":"Virus Genes"},{"issue":"1","key":"10.1016\/j.jviromet.2019.113796_bib0060","first-page":"164","article-title":"Evidence on Strawberry latent ringspot virus infections in fig (Ficus carica)","volume":"54","author":"Elbeaino","year":"2015","journal-title":"Phytopathol. Medit."},{"issue":"11","key":"10.1016\/j.jviromet.2019.113796_bib0065","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1099\/jgv.0.001143","article-title":"ICTV report consortium (2018). ICTV virus taxonomy profile: fimoviridae","volume":"99","author":"Elbeaino","year":"2018","journal-title":"J. Gen. Virol."},{"issue":"3","key":"10.1016\/j.jviromet.2019.113796_bib0070","article-title":"Some properties of Fig latent virus 1, a new member of the family Flexiviridae","volume":"91","author":"Gattoni","year":"2009","journal-title":"J. Plant Pathol."},{"issue":"4\u20135","key":"10.1016\/j.jviromet.2019.113796_bib0075","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1007\/s11103-015-0282-5","article-title":"Camelid nanobodies with high affinity for broad bean mottle virus: a possible promising tool to immunomodulate plant resistance against viruses","volume":"87","author":"Ghannam","year":"2015","journal-title":"Plant Mol. Biol."},{"issue":"3","key":"10.1016\/j.jviromet.2019.113796_bib0080","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1111\/j.1432-1033.1997.00633.x","article-title":"Identification of phosphopeptide ligands for the Src\u2010homology 2 (SH2) domain of Grb2 by phage display","volume":"246","author":"Gram","year":"1997","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.jviromet.2019.113796_bib0085","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1002\/elps.1150091106","article-title":"Extraction of plant proteins for two-dimensional electrophoresis","volume":"9","author":"Granier","year":"1988","journal-title":"Electrophoresis"},{"issue":"14","key":"10.1016\/j.jviromet.2019.113796_bib0090","doi-asserted-by":"crossref","first-page":"3245","DOI":"10.1002\/j.1460-2075.1994.tb06626.x","article-title":"Isolation of high affinity human antibodies directly from large synthetic repertoires","volume":"13","author":"Griffiths","year":"1994","journal-title":"EMBO"},{"key":"10.1016\/j.jviromet.2019.113796_bib0095","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1111\/pbi.12819","article-title":"Nanobody-mediated resistance to Grapevine fanleaf virus in plants","volume":"16","author":"Hemmer","year":"2018","journal-title":"Plant Biotechnol. J."},{"issue":"1","key":"10.1016\/j.jviromet.2019.113796_bib0100","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1380-2933(98)00007-4","article-title":"Antibody phage display technology and its applications","volume":"4","author":"Hoogenboom","year":"1998","journal-title":"Immunotechnology"},{"issue":"6","key":"10.1016\/j.jviromet.2019.113796_bib0105","doi-asserted-by":"crossref","first-page":"7038","DOI":"10.3390\/ijms13067038","article-title":"Generation of a highly reactive chicken-derived single-chain variable fragment against Fusarium verticillioides by phage display","volume":"13","author":"Hu","year":"2012","journal-title":"Int. J. Mol. Sci."},{"issue":"1","key":"10.1016\/j.jviromet.2019.113796_bib0110","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1128\/JVI.02527-14","article-title":"Nucleocapsid protein from fig mosaic virus forms cytoplasmic agglomerates that are hauled by endoplasmic reticulum streaming","volume":"89","author":"Ishikawa","year":"2015","journal-title":"J. Virol."},{"issue":"5","key":"10.1016\/j.jviromet.2019.113796_bib0115","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1089\/hyb.2009.0004","article-title":"Isolation of BNYVV coat protein-specific single chain Fv from a mouse phage library antibody","volume":"28","author":"Jahromi","year":"2009","journal-title":"Hybridoma"},{"key":"10.1016\/j.jviromet.2019.113796_bib0120","doi-asserted-by":"crossref","first-page":"656","DOI":"10.3389\/fpls.2019.00656","article-title":"In silico prediction and validations of domains involved in Gossypium hirsutum SnRK1 protein interaction with cotton leaf curl multan betasatellite encoded C1","volume":"10","author":"Kamal","year":"2019","journal-title":"Front. Plant Sci."},{"issue":"1","key":"10.1016\/j.jviromet.2019.113796_bib0125","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.jim.2011.01.006","article-title":"Isolation and characterization of recombinant single chain fragment variable anti-idiotypic antibody specific to Aspergillus fumigatus membrane protein","volume":"366","author":"Krishnaswamy","year":"2011","journal-title":"J. Immunol. Methods"},{"issue":"12","key":"10.1016\/j.jviromet.2019.113796_bib0130","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1094\/PHYTO-12-11-0351","article-title":"An integrated badnavirus is prevalent in fig germplasm","volume":"102","author":"Laney","year":"2012","journal-title":"Phytopathology"},{"issue":"9","key":"10.1016\/j.jviromet.2019.113796_bib0135","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1094\/MPMI-21-9-1242","article-title":"Engineering Fusarium head blight resistance in wheat by expression of a fusion protein containing a Fusarium-specific antibody and an antifungal peptide","volume":"21","author":"Li","year":"2008","journal-title":"Mol. Plant Microbe Interact."},{"issue":"7","key":"10.1016\/j.jviromet.2019.113796_bib0140","doi-asserted-by":"crossref","first-page":"3950","DOI":"10.1074\/jbc.M114.604678","article-title":"Structure and function analysis of nucleocapsid protein of tomato spotted wilt tospovirus interacting with RNA using homology modeling","volume":"290","author":"Li","year":"2014","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jviromet.2019.113796_bib0145","series-title":"Diseases of Tropical Fruit Crops","first-page":"253","article-title":"Diseases of fig","author":"Michailides","year":"2003"},{"issue":"4","key":"10.1016\/j.jviromet.2019.113796_bib0150","doi-asserted-by":"crossref","first-page":"357","DOI":"10.2478\/jppr-2013-0054","article-title":"Selection of specific single chain variable fragments (Scfv) against Polymyxa betae from phage display libraries","volume":"53","author":"Safarnejad","year":"2013","journal-title":"J. Plant Prot. Res."},{"issue":"2","key":"10.1016\/j.jviromet.2019.113796_bib0155","first-page":"311","article-title":"Generation and characterization of functional recombinant antibody fragments against tomato yellow leaf curl virus replication-associated protein","volume":"73","author":"Safarnejad","year":"2008","journal-title":"Comm. Agric. Appl. Biol. Sci."},{"key":"10.1016\/j.jviromet.2019.113796_bib0160","first-page":"93","article-title":"Development of fig cultivation (Ficus carica) for fresh and processed consumption, as an alternative of diversification for the agricultural sector","author":"Segreda","year":"2007","journal-title":"Component II: Fig Agroind."},{"key":"10.1016\/j.jviromet.2019.113796_bib0165","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.jviromet.2018.12.016","article-title":"Production of a polyclonal antiserum against recombinant nucleocapsid protein and its application for the detection of fig mosaic virus","volume":"265","author":"Shahmirzaie","year":"2019","journal-title":"J. Virol. Methods"},{"issue":"1","key":"10.1016\/j.jviromet.2019.113796_bib0170","doi-asserted-by":"crossref","first-page":"14","DOI":"10.5812\/ijb.9305","article-title":"Preparation of antibody against immunodominant membrane protein (IMP) of Candidatus Phytoplasma aurantifolia","volume":"11","author":"Shahryari","year":"2013","journal-title":"Iranian J. Biotechnol."},{"key":"10.1016\/j.jviromet.2019.113796_bib0175","series-title":"Improvement of Crop Plants for Industrial End Uses","first-page":"435","article-title":"Molecular farming of antibodies in plants","author":"Twyman","year":"2007"},{"issue":"3","key":"10.1016\/j.jviromet.2019.113796_bib0180","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1007\/s11103-005-4091-0","article-title":"Immunomodulation of cucumber mosaic virus infection by intrabodies selected in vitro from a stable single-framework phage display library","volume":"58","author":"Villani","year":"2005","journal-title":"Plant Mol. Biol."},{"issue":"1","key":"10.1016\/j.jviromet.2019.113796_bib0185","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1146\/annurev.iy.12.040194.002245","article-title":"Making antibodies by phage display technology","volume":"12","author":"Winter","year":"1994","journal-title":"Ann. Rev. Immunol."},{"issue":"1","key":"10.1016\/j.jviromet.2019.113796_bib0190","doi-asserted-by":"crossref","first-page":"807","DOI":"10.3390\/molecules20010807","article-title":"Design, synthesis and anti-tobacco mosaic virus (TMV) activity of 5-Chloro-N-(4-cyano-1-aryl-1H-pyrazol-5-yl)-1-aryl-3-methyl-1H-pyrazole-4-carboxamide derivatives","volume":"20","author":"Xiao","year":"2015","journal-title":"Molecules"},{"issue":"20","key":"10.1016\/j.jviromet.2019.113796_bib0195","doi-asserted-by":"crossref","first-page":"9455","DOI":"10.1021\/jf801768g","article-title":"Detection of Sclerotinia sclerotiorum using a monomeric and dimeric single-chain fragment variable (scFv) antibody","volume":"56","author":"Yajima","year":"2008","journal-title":"J. Agric. Food Chem."},{"issue":"9","key":"10.1016\/j.jviromet.2019.113796_bib0200","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.tim.2013.07.006","article-title":"Structural perspective on the formation of ribonucleoprotein complex in negative-sense single-stranded RNA viruses","volume":"21","author":"Zhou","year":"2013","journal-title":"Trends Microbiol."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093419303623?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093419303623?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T10:52:43Z","timestamp":1759056763000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093419303623"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2]]},"references-count":40,"alternative-id":["S0166093419303623"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2019.113796","relation":{},"ISSN":["0166-0934"],"issn-type":[{"type":"print","value":"0166-0934"}],"subject":[],"published":{"date-parts":[[2020,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Generation and molecular docking analysis of specific single-chain variable fragments selected by phage display against the recombinant nucleocapsid protein of fig mosaic virus","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2019.113796","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"113796"}}