{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T15:53:11Z","timestamp":1778860391512,"version":"3.51.4"},"reference-count":22,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2022,5]]},"DOI":"10.1016\/j.jviromet.2022.114503","type":"journal-article","created":{"date-parts":[[2022,2,24]],"date-time":"2022-02-24T13:02:56Z","timestamp":1645707776000},"page":"114503","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Discrepant results of hepatitis B virus genotype determination by PCR and DNA sequencing"],"prefix":"10.1016","volume":"303","author":[{"given":"Yihan","family":"Xiao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lan","family":"Ni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhigang","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Long","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojun","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinjun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lihua","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuanning","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feifei","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina","family":"Niu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuji","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunfan","family":"Quan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2022.114503_bib1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1001\/jama.295.1.65","article-title":"Risk of hepatocellular carcinoma across a biological gradient of serum hepatitis B virus DNA level","volume":"295","author":"Chen","year":"2006","journal-title":"JAMA"},{"key":"10.1016\/j.jviromet.2022.114503_bib2","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.jcv.2006.12.007","article-title":"Improved multiplex-PCR to identify hepatitis B virus genotypes A-F and subgenotypes B1, B2, C1 and C2","volume":"38","author":"Chen","year":"2007","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jviromet.2022.114503_bib3","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1128\/jvi.47.2.337-343.1983","article-title":"Structure of the hepatitis B virus genome","volume":"47","author":"Delius","year":"1983","journal-title":"J.Virol."},{"key":"10.1016\/j.jviromet.2022.114503_bib4","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/j.jhep.2017.03.021","article-title":"EASL 2017 Clinical Practice Guidelines on the management of hepatitis B virus infection","volume":"67","author":"EASL","year":"2017","journal-title":"J. Hepatol."},{"key":"10.1016\/j.jviromet.2022.114503_bib5","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a021386","article-title":"Hepadnavirus Genome Replication and Persistence","volume":"5","author":"Hu","year":"2015","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"10.1016\/j.jviromet.2022.114503_bib6","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1002\/hep.26635","article-title":"Risk factors for long-term persistence of serum hepatitis B surface antigen following acute hepatitis B virus infection in Japanese adults","volume":"59","author":"Ito","year":"2014","journal-title":"Hepatology"},{"key":"10.1016\/j.jviromet.2022.114503_bib7","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1046\/j.1440-1746.2002.02737.x","article-title":"Hepatitis B viral genotypes: clinical relevance and molecular characteristics","volume":"17","author":"Kao","year":"2002","journal-title":"J. Gastroenterol. Hepatol."},{"key":"10.1016\/j.jviromet.2022.114503_bib8","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1002\/jmv.10534","article-title":"Hepatitis B virus genotypes and spontaneous hepatitis B e antigen seroconversion in Taiwanese hepatitis B carriers","volume":"72","author":"Kao","year":"2004","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jviromet.2022.114503_bib9","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/S1386-6532(03)00084-2","article-title":"A multiplex-PCR to identify hepatitis B virus--enotypes A-F","volume":"29","author":"Kirschberg","year":"2004","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jviromet.2022.114503_bib10","doi-asserted-by":"crossref","first-page":"S9","DOI":"10.1111\/j.1872-034X.2007.00098.x","article-title":"Epidemiology of hepatitis B virus in Africa, its genotypes and clinical associations of genotypes","volume":"37","author":"Kramvis","year":"2007","journal-title":"Hepatol. Res."},{"key":"10.1016\/j.jviromet.2022.114503_bib11","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1016\/0140-6736(93)92249-S","article-title":"Molecular virology and pathogenesis of hepatitis B","volume":"342","author":"Lau","year":"1993","journal-title":"Lancet"},{"key":"10.1016\/j.jviromet.2022.114503_bib12","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a021436","article-title":"Hepatitis B virus genotypes and variants","volume":"5","author":"Lin","year":"2015","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"10.1016\/j.jviromet.2022.114503_bib13","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1086\/509894","article-title":"Hepatitis B virus genotypes in Alaska Native people with hepatocellular carcinoma: preponderance of genotype F","volume":"195","author":"Livingston","year":"2007","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jviromet.2022.114503_bib14","doi-asserted-by":"crossref","DOI":"10.1002\/hep.32065","article-title":"Hepatitis B Virus (HBV) Genotype: A Significant Risk Factor in Determining which Patients with Chronic HBV Infection should Undergo Surveillance for Hepatocellular Carcinoma: The Hepatitis B Alaska (HEP-B-AK) Study","author":"McMahon","year":"2021","journal-title":"Hepatology"},{"key":"10.1016\/j.jviromet.2022.114503_bib15","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1099\/jgv.0.001387","article-title":"Analysis of genomic-length HBV sequences to determine genotype and subgenotype reference sequences","volume":"101","author":"McNaughton","year":"2020","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2022.114503_bib16","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1128\/JCM.39.1.362-364.2001","article-title":"Rapid and specific genotyping system for hepatitis B virus corresponding to six major genotypes by PCR using type-specific primers","volume":"39","author":"Naito","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jviromet.2022.114503_bib17","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1053\/jhep.2001.27221","article-title":"Geographic distribution of hepatitis B virus (HBV) genotype in patients with chronic HBV infection in Japan","volume":"34","author":"Orito","year":"2001","journal-title":"Hepatology"},{"key":"10.1016\/j.jviromet.2022.114503_bib18","doi-asserted-by":"crossref","first-page":"7152","DOI":"10.3748\/wjg.v20.i23.7152","article-title":"Molecular identification of hepatitis B virus genotypes\/subgenotypes: revised classification hurdles and updated resolutions","volume":"20","author":"Pourkarim","year":"2014","journal-title":"World J. Gastroenterol."},{"key":"10.1016\/j.jviromet.2022.114503_bib19","doi-asserted-by":"crossref","first-page":"W654","DOI":"10.1093\/nar\/gkh419","article-title":"A web-based genotyping resource for viral sequences","volume":"32","author":"Rozanov","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jviromet.2022.114503_bib20","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/0092-8674(82)90157-X","article-title":"Replication of the genome of a hepatitis B--like virus by reverse transcription of an RNA intermediate","volume":"29","author":"Summers","year":"1982","journal-title":"Cell"},{"key":"10.1016\/j.jviromet.2022.114503_bib21","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1002\/hep.27406","article-title":"The global burden of liver disease: the major impact of China","volume":"60","author":"Wang","year":"2014","journal-title":"Hepatology"},{"key":"10.1016\/j.jviromet.2022.114503_bib22","unstructured":"WHO, 2021, Hepatitis B. \u3008https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/hepatitis-b\u3009."}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093422000507?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093422000507?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T10:55:06Z","timestamp":1759056906000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093422000507"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5]]},"references-count":22,"alternative-id":["S0166093422000507"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2022.114503","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-1094883\/v1","asserted-by":"object"}]},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2022,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Discrepant results of hepatitis B virus genotype determination by PCR and DNA sequencing","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2022.114503","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"114503"}}