{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:01:18Z","timestamp":1790053278313,"version":"4.0.1"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T00:00:00Z","timestamp":1719619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["101079884"],"award-info":[{"award-number":["101079884"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research and Innovation Agency","doi-asserted-by":"publisher","award":["P3-0083"],"award-info":[{"award-number":["P3-0083"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research and Innovation Agency","doi-asserted-by":"publisher","award":["J3-3062"],"award-info":[{"award-number":["J3-3062"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2024,9]]},"DOI":"10.1016\/j.jviromet.2024.114993","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T12:11:25Z","timestamp":1719835885000},"page":"114993","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["A novel digital PCR assay for detection and comprehensive characterization of Molluscum contagiosum virus genotypes MOCV1, MOCV2, and MOCV3 and recombinant lineages"],"prefix":"10.1016","volume":"329","author":[{"given":"Toma\u017e M.","family":"Zorec","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucijan","family":"Skubic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Poljak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2024.114993_bib1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1002\/jmv.2124","article-title":"Molecular epidemiology of molluscum contagiosum virus and analysis of the host-serum antibody response in Spanish HIV-negative patients","volume":"66","author":"Agromayor","year":"2002","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jviromet.2024.114993_bib2","doi-asserted-by":"crossref","first-page":"3583","DOI":"10.1128\/jvi.65.7.3583-3589.1991","article-title":"Characterization of a molluscum contagiosum virus homolog of the vaccinia virus p37K major envelope antigen","volume":"65","author":"Blake","year":"1991","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2024.114993_bib3","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.01526-22","article-title":"Molecular mechanisms of poxvirus evolution","volume":"14","author":"Brennan","year":"2023","journal-title":"mBio"},{"key":"10.1016\/j.jviromet.2024.114993_bib4","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/S1473-3099(13)70109-9","article-title":"Molluscum contagiosum virus infection","volume":"13","author":"Chen","year":"2013","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.jviromet.2024.114993_bib5","doi-asserted-by":"crossref","DOI":"10.1099\/jgv.0.001357","article-title":"Molecular and genomic characterization of a novel equine molluscum contagiosum-like virus","volume":"102","author":"Ehmann","year":"2021","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2024.114993_bib6","doi-asserted-by":"crossref","first-page":"957","DOI":"10.3892\/ijmm.2020.4673","article-title":"Sensitivity assessment of droplet digital PCR for SARS-CoV-2 detection","volume":"46","author":"Falzone","year":"2020","journal-title":"Int. J. Mol. Med."},{"key":"10.1016\/j.jviromet.2024.114993_bib7","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/s40257-023-00778-4","article-title":"Differential diagnosis, prevention, and treatment of mpox (monkeypox): A review for dermatologists","volume":"24","author":"Gupta","year":"2023","journal-title":"Am. J. Clin. Dermatol."},{"key":"10.1016\/j.jviromet.2024.114993_bib8","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1038\/jid.2013.446","article-title":"The global burden of skin disease in 2010: an analysis of the prevalence and impact of skin conditions","volume":"134","author":"Hay","year":"2014","journal-title":"J. Invest. Dermatol."},{"key":"10.1016\/j.jviromet.2024.114993_bib9","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.jviromet.2012.11.008","article-title":"Rapid detection and typing of Molluscum contagiosum virus by FRET-based real-time PCR","volume":"187","author":"Ho\u0161njak","year":"2013","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2024.114993_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.amsu.2022.104069","article-title":"The outbreak of monkeypox 2022: An overview","volume":"79","author":"Hraib","year":"2022","journal-title":"Ann. Med. Surg."},{"key":"10.1016\/j.jviromet.2024.114993_bib11","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT multiple sequence alignment software version 7: improvements in performance and usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.jviromet.2024.114993_bib12","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1086\/314620","article-title":"Molluscum contagiosum virus: antibody responses in persons with clinical lesions and seroepidemiology in a representative Australian population","volume":"179","author":"Konya","year":"1999","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jviromet.2024.114993_bib13","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1093\/bioinformatics\/btm091","article-title":"Enhancements and modifications of primer design program Primer3","volume":"23","author":"Koressaar","year":"2007","journal-title":"Bioinformatics"},{"key":"10.1016\/j.jviromet.2024.114993_bib14","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1099\/jgv.0.000759","article-title":"Recombination events and variability among full-length genomes of co-circulating molluscum contagiosum virus subtypes 1 and 2","volume":"98","author":"L\u00f3pez-Bueno","year":"2017","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.jviromet.2024.114993_bib15","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1002\/jmv.1890460409","article-title":"Analysis of molluscum contagiosum virus genomes isolated in Japan","volume":"46","author":"Nakamura","year":"1995","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jviromet.2024.114993_bib16","unstructured":"NCBI, 2023. GenBank. \u3008https:\/\/www.ncbi.nlm.nih.gov\/genbank\/\u3009 (accessed 10 November 2023)."},{"key":"10.1016\/j.jviromet.2024.114993_bib17","unstructured":"NCBI, 2023. Nucleotide BLAST. \u3008https:\/\/blast.ncbi.nlm.nih.gov\/Blast.cgi\u3009 (accessed 22 November 2023)."},{"key":"10.1016\/j.jviromet.2024.114993_bib18","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1002\/(SICI)1096-9071(199612)50:4<342::AID-JMV10>3.0.CO;2-K","article-title":"Detection and typing of molluscum contagiosum virus in skin lesions by using a simple lysis method and polymerase chain reaction","volume":"50","author":"Nu\u00f1ez","year":"1996","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jviromet.2024.114993_bib19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jmv.1890380102","article-title":"Characterisation by restriction mapping of three subtypes of molluscum contagiosum virus","volume":"38","author":"Porter","year":"1992","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jviromet.2024.114993_bib20","unstructured":"PREMIER Biosoft, 2023. Net Primer. \u3008http:\/\/www.premierbiosoft.com\/netprimer\/\u3009 (accessed 22 November 2023)."},{"key":"10.1016\/j.jviromet.2024.114993_bib21","first-page":"111","article-title":"Molecular epidemiology of the 2022 monkeypox virus outbreak in Slovenia","volume":"32","author":"Resman Rus","year":"2023","journal-title":"Acta Derm. Alp. Pannonica Adriat."},{"key":"10.1016\/j.jviromet.2024.114993_bib22","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.3390\/s18041271","article-title":"dPCR: A Technology Review","volume":"18","author":"Quan","year":"2018","journal-title":"Sens. (Basel)"},{"key":"10.1016\/j.jviromet.2024.114993_bib23","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.arcmed.2005.05.020","article-title":"Detection of Molluscum contagiosum virus (MCV) subtype I as a single dominant virus subtype in Molluscum lesions from a Turkish population","volume":"37","author":"Saral","year":"2006","journal-title":"Arch. Med. Res."},{"key":"10.1016\/j.jviromet.2024.114993_bib24","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1126\/science.273.5276.813","article-title":"Genome sequence of a human tumorigenic poxvirus: prediction of specific host response-evasion genes","volume":"273","author":"Senkevich","year":"1996","journal-title":"Science"},{"key":"10.1016\/j.jviromet.2024.114993_bib25","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1006\/viro.1997.8607","article-title":"The genome of molluscum contagiosum virus: analysis and comparison with other poxviruses","volume":"233","author":"Senkevich","year":"1997","journal-title":"Virology"},{"key":"10.1016\/j.jviromet.2024.114993_bib26","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0088734","article-title":"Seroprevalence of Molluscum contagiosum virus in German and UK populations","volume":"9","author":"Sherwani","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.jviromet.2024.114993_bib27","first-page":"129","article-title":"Molecular screening and single nucleotide polymorphism typing of molluscum contagiosum virus (MCV) from genital specimens, between 2012 and 2015","volume":"22","author":"Taghinezhad-S","year":"2018","journal-title":"Iran. Biomed. J."},{"key":"10.1016\/j.jviromet.2024.114993_bib28","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1002\/(SICI)1096-9071(199711)53:3<205::AID-JMV4>3.0.CO;2-C","article-title":"Identification and typing of molluscum contagiosum virus in clinical specimens by polymerase chain reaction","volume":"53","author":"Thompson","year":"1997","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.jviromet.2024.114993_bib29","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.jcv.2007.09.007","article-title":"Identification and genotyping of molluscum contagiosum virus from genital swab samples by real-time PCR and Pyrosequencing","volume":"40","author":"Trama","year":"2007","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jviromet.2024.114993_bib30","first-page":"130","article-title":"Clinical and demographic characteristics of patients with molluscum contagiosum treated at the University Dermatology Clinic Maribor in a 5-year period","volume":"24","author":"Tr\u010dko","year":"2016","journal-title":"Acta Dermatovenerol. Croat."},{"key":"10.1016\/j.jviromet.2024.114993_bib31","doi-asserted-by":"crossref","DOI":"10.1093\/ofid\/ofy298","article-title":"Clinical, histopathological, and virological evaluation of 203 patients with a clinical diagnosis of molluscum contagiosum","volume":"5","author":"Tr\u010dko","year":"2018","journal-title":"Open Forum Infect. Dis."},{"key":"10.1016\/j.jviromet.2024.114993_bib32","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gks596","article-title":"Primer3--new capabilities and interfaces.","volume":"40","author":"Untergasser","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.jviromet.2024.114993_bib33","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/j.1365-4362.1996.tb03270.x","article-title":"Molecular epidemiologic analysis of Japanese patients with molluscum contagiosum","volume":"35","author":"Yamashita","year":"1996","journal-title":"Int. J. Dermatol."},{"key":"10.1016\/j.jviromet.2024.114993_bib34","doi-asserted-by":"crossref","first-page":"586","DOI":"10.3390\/v10110586","article-title":"New insights into the evolutionary and genomic landscape of molluscum contagiosum virus (MCV) based on nine MCV1 and six MCV2 complete genome sequences","volume":"10","author":"Zorec","year":"2018","journal-title":"Viruses"},{"key":"10.1016\/j.jviromet.2024.114993_bib35","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.02224-23","volume":"14","author":"Zorec","year":"2023","journal-title":"mBio"}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093424001174?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093424001174?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:05:17Z","timestamp":1777496717000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093424001174"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":35,"alternative-id":["S0166093424001174"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2024.114993","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A novel digital PCR assay for detection and comprehensive characterization of Molluscum contagiosum virus genotypes MOCV1, MOCV2, and MOCV3 and recombinant lineages","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2024.114993","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"114993"}}