{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T09:10:11Z","timestamp":1756717811853,"version":"3.44.0"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T00:00:00Z","timestamp":1755820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.jviromet.2025.115246","type":"journal-article","created":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T15:12:34Z","timestamp":1755961954000},"page":"115246","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Evaluation of optimized ELISA to detect anti-cytomegalovirus antibodies against immunogenic proteins"],"prefix":"10.1016","volume":"338","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2101-8800","authenticated-orcid":false,"given":"Abdulrahim R.","family":"Hakami","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatimah A.","family":"Alshehri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irfan","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Essa N.","family":"Shamakhi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed R.","family":"Gosady","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hussain M.","family":"Qusadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali Y.","family":"Mujayridi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander W.","family":"Tarr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gasim","family":"Dobie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2025.115246_bib1","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens9100803","article-title":"Development and optimization of In-house ELISA for detection of human IgG antibody to SARS-CoV-2 full length spike protein","volume":"9","author":"Alandijany","year":"2020","journal-title":"Pathogens"},{"key":"10.1016\/j.jviromet.2025.115246_bib2","first-page":"388","article-title":"Seroprevalence of human cytomegalovirus antibodies among children with type I diabetes mellitus in the aseer region, southwest KSA","volume":"11","author":"Al-Hakami","year":"2016","journal-title":"J. Taibah University Med. Sci. Spec. Issue Diabetes Mellitus Arab World"},{"key":"10.1016\/j.jviromet.2025.115246_bib3","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.3390\/diagnostics12071725","article-title":"Clinical and serological findings of COVID-19 participants in the region of makkah, Saudi Arabia","volume":"12","author":"Alzahrani","year":"2022","journal-title":"Diagnostics"},{"issue":"1","key":"10.1016\/j.jviromet.2025.115246_bib4","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.bjm.2018.05.001","article-title":"Development of an indirect ELISA based on recombinant capsid protein to detect antibodies to bovine leukemia virus","volume":"49","author":"Andreolla","year":"2018","journal-title":"Braz. J. Microbiol"},{"key":"10.1016\/j.jviromet.2025.115246_bib5","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0010152","article-title":"Diagnostic accuracy of serological tests for the diagnosis of chikungunya virus infection: a systematic review and meta-analysis","volume":"16","author":"Andrew","year":"2022","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.jviromet.2025.115246_bib6","doi-asserted-by":"crossref","first-page":"185","DOI":"10.2147\/OPTH.S80475","article-title":"Herpes simplex keratitis: challenges in diagnosis and clinical management","volume":"11","author":"Azher","year":"2017","journal-title":"Clin. Ophthalmol."},{"key":"10.1016\/j.jviromet.2025.115246_bib7","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.jcv.2011.05.007","article-title":"Problems and challenges in the diagnosis of vertical infection with human cytomegalovirus (CMV): lessons from two accidental cases","volume":"51","author":"Berger","year":"2011","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jviromet.2025.115246_bib8","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/1743-422X-6-45","article-title":"Highly quantitative serological detection of anti-cytomegalovirus (CMV) antibodies","volume":"6","author":"Burbelo","year":"2009","journal-title":"Virol. J."},{"key":"10.1016\/j.jviromet.2025.115246_bib9","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s12985-021-01568-2","article-title":"Development of an indirect ELISA to specifically detect antibodies against African swine fever virus: bioinformatics approaches","volume":"18","author":"Gao","year":"2021","journal-title":"Virol. J."},{"key":"10.1016\/j.jviromet.2025.115246_bib10","doi-asserted-by":"crossref","first-page":"6832","DOI":"10.3390\/ijms24076832","article-title":"Effect of cytomegalovirus reactivation on inflammatory status and mortality of older COVID-19 patients","volume":"24","author":"Giacconi","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jviromet.2025.115246_bib11","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.jim.2013.11.014","article-title":"Non-specific binding in solid phase immunoassays for autoantibodies correlates with inflammation markers","volume":"403","author":"G\u00fcven","year":"2014","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jviromet.2025.115246_bib12","first-page":"172","article-title":"Effects of photobiomodulation therapy on glycemic and lipid profiles in vitro","volume":"9","author":"Hakami","year":"2020","journal-title":"Med. Lasers Eng. Basic Res. Clin. Appl."},{"key":"10.1016\/j.jviromet.2025.115246_bib13","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1186\/s12985-021-01660-7","article-title":"Detection of chikungunya virus in the Southern region, Saudi Arabia","volume":"18","author":"Hakami","year":"2021","journal-title":"Virol. J."},{"key":"10.1016\/j.jviromet.2025.115246_bib14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jviromet.2015.04.023","article-title":"Non-ionic detergents facilitate non-specific binding of M13 bacteriophage to polystyrene surfaces","volume":"221","author":"Hakami","year":"2015","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2025.115246_bib15","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.jim.2019.01.005","article-title":"Development and validation of different indirect ELISAs for MERS-CoV serological testing","volume":"466","author":"Hashem","year":"2019","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jviromet.2025.115246_bib16","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1155\/2007\/604727","article-title":"Herpes simplex virus type-specific serology: where does it fit in the diagnostic armamentarium?","volume":"18","author":"Hatchette","year":"2007","journal-title":"Can. J. Infect. Dis. Med Microbiol"},{"key":"10.1016\/j.jviromet.2025.115246_bib17","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1111\/1348-0421.12683","article-title":"Evaluation of the indirect and IgM-capture anti-human cytomegalovirus IgM ELISA methods as confirmed by cytomegalovirus IgG avidity","volume":"63","author":"Ikuta","year":"2019","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.jviromet.2025.115246_bib18","doi-asserted-by":"crossref","first-page":"1708","DOI":"10.3390\/foods10081708","article-title":"Non-Specific binding and Cross-Reaction of ELISA: a case study of porcine hemoglobin detection","volume":"10","author":"Jiang","year":"2021","journal-title":"Foods"},{"key":"10.1016\/j.jviromet.2025.115246_bib19","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1128\/MMBR.00040-07","article-title":"Tegument proteins of human cytomegalovirus","volume":"72","author":"Kalejta","year":"2008","journal-title":"Microbiol Mol. Biol. Rev."},{"key":"10.1016\/j.jviromet.2025.115246_bib20","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1186\/s12879-017-2777-y","article-title":"Diagnostic accuracy of serological diagnosis of hepatitis c and b using dried blood spot samples (DBS): two systematic reviews and meta-analyses","volume":"17","author":"Lange","year":"2017","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.jviromet.2025.115246_bib21","doi-asserted-by":"crossref","first-page":"m2516","DOI":"10.1136\/bmj.m2516","article-title":"Diagnostic accuracy of serological tests for covid-19: systematic review and meta-analysis","author":"Lisboa Bastos","year":"2020","journal-title":"BMJ 370"},{"key":"10.1016\/j.jviromet.2025.115246_bib22","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00964-21","article-title":"A novel multiplexed Enzyme-Linked immunosorbent assay for the detection of IgG seroreactivity to cytomegalovirus (CMV) UL144","volume":"59","author":"Miller","year":"2021","journal-title":"J. Clin. Microbiol"},{"key":"10.1016\/j.jviromet.2025.115246_bib23","doi-asserted-by":"crossref","first-page":"313","DOI":"10.3390\/diagnostics12020313","article-title":"Development of an automated chemiluminescent enzyme immunoassay for measuring thrombopoietin in human plasma","volume":"12","author":"Nishikawa","year":"2022","journal-title":"Diagnostics"},{"key":"10.1016\/j.jviromet.2025.115246_bib24","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1128\/CVI.00281-08","article-title":"Enzyme-Linked immunosorbent assay method for detection of cytomegalovirus Strain-Specific antibody responses","volume":"16","author":"Novak","year":"2009","journal-title":"Clin. Vaccin. Immunol."},{"key":"10.1016\/j.jviromet.2025.115246_bib25","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.jcv.2017.10.004","article-title":"Optimized enzyme-linked immunosorbent assay for detecting cytomegalovirus infections during clinical trials of recombinant vaccines","volume":"96","author":"Pagnon","year":"2017","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.jviromet.2025.115246_bib26","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1155\/2007\/505364","article-title":"The diagnosis of genital herpes - beyond culture: an evidence-based guide for the utilization of polymerase chain reaction and herpes simplex virus type-specific serology","volume":"18","author":"Ratnam","year":"2007","journal-title":"Can. J. Infect. Dis. Med Microbiol"},{"key":"10.1016\/j.jviromet.2025.115246_bib27","doi-asserted-by":"crossref","first-page":"S74","DOI":"10.1093\/infdis\/jiz601","article-title":"Clinical diagnostic testing for human cytomegalovirus infections","volume":"221","author":"Razonable","year":"2020","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jviromet.2025.115246_bib28","doi-asserted-by":"crossref","first-page":"466","DOI":"10.2174\/187152611797636703","article-title":"Overview of the diagnosis of cytomegalovirus infection","volume":"11","author":"Ross","year":"2011","journal-title":"Infect. Disord. Drug Targets"},{"key":"10.1016\/j.jviromet.2025.115246_bib29","doi-asserted-by":"crossref","first-page":"774","DOI":"10.3390\/pathogens12060774","article-title":"Development of a novel indirect ELISA for the serological diagnosis of African swine fever using p11.5 protein as a target antigen","volume":"12","author":"Watanabe","year":"2023","journal-title":"Pathogens"},{"key":"10.1016\/j.jviromet.2025.115246_bib30","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.jviromet.2016.11.001","article-title":"Establishment and validation of an enzyme-linked immunosorbent assay for IgG antibody against cytomegalovirus based on pp150 antigen","volume":"240","author":"Xi","year":"2017","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2025.115246_bib31","doi-asserted-by":"crossref","first-page":"221","DOI":"10.3390\/pathogens13030221","article-title":"Development of a Double-Antibody sandwich ELISA based on a monoclonal antibody against the viral NS1 protein for the detection of chicken parvovirus","volume":"13","author":"Zhang","year":"2024","journal-title":"Pathogens"}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093425001399?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093425001399?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T08:47:35Z","timestamp":1756716455000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093425001399"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":31,"alternative-id":["S0166093425001399"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2025.115246","relation":{},"ISSN":["0166-0934"],"issn-type":[{"type":"print","value":"0166-0934"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Evaluation of optimized ELISA to detect anti-cytomegalovirus antibodies against immunogenic proteins","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2025.115246","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"115246"}}