{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T14:07:38Z","timestamp":1785247658448,"version":"3.55.0"},"reference-count":22,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100016099","name":"Jiangsu Agri-animal Husbandry Vocational College","doi-asserted-by":"publisher","award":["NSF2023TC02"],"award-info":[{"award-number":["NSF2023TC02"]}],"id":[{"id":"10.13039\/501100016099","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Virological Methods"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.jviromet.2025.115306","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T16:36:53Z","timestamp":1763656613000},"page":"115306","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Development of monoclonal antibodies against goose astrovirus 2 ORF2 protein and establishment of an indirect competitive ELISA detection method"],"prefix":"10.1016","volume":"340","author":[{"given":"Anping","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingkang","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanyuan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jviromet.2025.115306_bib1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3390\/v9010015","article-title":"The astrovirus capsid: a review","volume":"9","author":"Arias","year":"2017","journal-title":"Viruses"},{"key":"10.1016\/j.jviromet.2025.115306_bib2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.3390\/vaccines7030079","article-title":"Immunogenicity and efficacy evaluation of subunit astrovirus vaccines","volume":"7","author":"Bidokhti","year":"2019","journal-title":"Vaccines"},{"key":"10.1016\/j.jviromet.2025.115306_bib3","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01859-16","article-title":"Structure of a human astrovirus capsid-antibody complex and mechanistic insights into virus neutralization","volume":"91","author":"Bogdanoff","year":"2017","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2025.115306_bib4","doi-asserted-by":"crossref","first-page":"579","DOI":"10.24425\/pjvs.2022.143541","article-title":"Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody","volume":"25","author":"Dai","year":"2022","journal-title":"Pol. J. Vet. Sci."},{"key":"10.1016\/j.jviromet.2025.115306_bib5","doi-asserted-by":"crossref","first-page":"7853","DOI":"10.1128\/JVI.03139-12","article-title":"Crystal structure of the avian astrovirus capsid spike","volume":"87","author":"DuBois","year":"2013","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2025.115306_bib6","doi-asserted-by":"crossref","first-page":"e2059","DOI":"10.1111\/tbed.14542","article-title":"Genetic diversity and evolution of goose astrovirus in the east of China","volume":"69","author":"Fei","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.jviromet.2025.115306_bib7","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.3390\/v14061306","article-title":"Insight into the epidemiology and evolutionary history of novel goose astrovirus-associated gout in goslings in Southern China","volume":"14","author":"Fu","year":"2022","journal-title":"Viruses"},{"key":"10.1016\/j.jviromet.2025.115306_bib8","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1186\/s12917-025-04687-y","article-title":"Identification of a novel linear B-cell epitope recognized by a monoclonal antibody against the capsid protein of goose astrovirus genotypes 2","volume":"21","author":"Li","year":"2025","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.jviromet.2025.115306_bib9","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1007\/s00253-021-11711-2","article-title":"Identification of three novel B cell epitopes in ORF2 protein of the emerging goose astrovirus and their application","volume":"106","author":"Ren","year":"2022","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jviromet.2025.115306_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.jviromet.2022.114646","article-title":"A peptide-based ELISA for detection of antibodies against novel goose astrovirus type 1","volume":"312","author":"Ren","year":"2023","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.jviromet.2025.115306_bib11","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01415-21","article-title":"Structures of two human astrovirus capsid\/neutralizing antibody complexes reveal distinct epitopes and inhibition of virus attachment to cells","volume":"96","author":"Ricemeyer","year":"2022","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2025.115306_bib12","doi-asserted-by":"crossref","first-page":"9008","DOI":"10.1128\/JVI.00694-16","article-title":"Crystal structure of the human astrovirus capsid protein","volume":"90","author":"Toh","year":"2016","journal-title":"J. Virol."},{"key":"10.1016\/j.jviromet.2025.115306_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2019.103766","article-title":"Specific detection of the novel goose astrovirus using a TaqMan real-time RT-PCR technology","volume":"137","author":"Wan","year":"2019","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.jviromet.2025.115306_bib14","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1007\/s00253-022-11852-y","article-title":"Core antigenic advantage domain-based ELISA to detect antibody against novel goose astrovirus in breeding geese","volume":"106","author":"Wang","year":"2022","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.jviromet.2025.115306_bib15","doi-asserted-by":"crossref","first-page":"e1865","DOI":"10.1111\/tbed.14522","article-title":"Genomic and epidemiological characteristics provide insights into the phylogeographic spread of goose astrovirus in China","volume":"69","author":"Wang","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.jviromet.2025.115306_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2024.104143","article-title":"Isolation, identification, and epidemiological characteristics of goose astrovirus causing acute gout in Guangdong province, China","volume":"103","author":"Xiang","year":"2024","journal-title":"Poult. Sci."},{"key":"10.1016\/j.jviromet.2025.115306_bib17","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1007\/s13205-019-2006-z","article-title":"A simple and rapid diagnostic method to detect new goose astrovirus using reverse-transcription loop-mediated isothermal amplification","volume":"10","author":"Yu","year":"2020","journal-title":"3 Biotech"},{"key":"10.1016\/j.jviromet.2025.115306_bib18","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00380-18","article-title":"Establishment and application of rapid diagnosis for reverse transcription-quantitative PCR of newly emerging goose-origin nephrotic astrovirus in China","volume":"3","author":"Yuan","year":"2018","journal-title":"mSphere"},{"key":"10.1016\/j.jviromet.2025.115306_bib19","article-title":"Surveillance and genetic diversity analysis of avian astrovirus in China","volume":"17","author":"Zhang","year":"2022","journal-title":"PLoS One"},{"key":"10.1016\/j.jviromet.2025.115306_bib20","article-title":"Development of a potential diagnostic monoclonal antibody against capsid spike protein VP27 of the novel goose astrovirus","volume":"101","author":"Zhang","year":"2022","journal-title":"Poult. Sci."},{"key":"10.1016\/j.jviromet.2025.115306_bib21","doi-asserted-by":"crossref","first-page":"664","DOI":"10.3390\/vaccines11030664","article-title":"Establishment and application of indirect ELISAs for detecting antibodies against goose astrovirus genotype 1 and 2","volume":"11","author":"Zhang","year":"2023","journal-title":"Vaccines"},{"key":"10.1016\/j.jviromet.2025.115306_bib22","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.3390\/v14081759","article-title":"Goose astrovirus in China: a comprehensive review","volume":"14","author":"Zhu","year":"2022","journal-title":"Viruses"}],"container-title":["Journal of Virological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093425001995?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166093425001995?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T05:27:13Z","timestamp":1773811633000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166093425001995"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":22,"alternative-id":["S0166093425001995"],"URL":"https:\/\/doi.org\/10.1016\/j.jviromet.2025.115306","relation":{},"ISSN":["0166-0934"],"issn-type":[{"value":"0166-0934","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of monoclonal antibodies against goose astrovirus 2 ORF2 protein and establishment of an indirect competitive ELISA detection method","name":"articletitle","label":"Article Title"},{"value":"Journal of Virological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jviromet.2025.115306","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"115306"}}