{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T17:01:54Z","timestamp":1784739714188,"version":"3.55.0"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T00:00:00Z","timestamp":1594166400000},"content-version":"vor","delay-in-days":189,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jvsvs.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["JVS-Vascular Science"],"published-print":{"date-parts":[[2020]]},"DOI":"10.1016\/j.jvssci.2020.07.002","type":"journal-article","created":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T02:50:36Z","timestamp":1595904636000},"page":"136-150","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Canonical transient receptor potential 6 channel deficiency promotes smooth muscle cells dedifferentiation and increased proliferation after arterial injury"],"prefix":"10.1016","volume":"1","author":[{"given":"Andrew H.","family":"Smith","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priya","family":"Putta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin C.","family":"Driscoll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinaki","family":"Chaudhuri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lutz","family":"Birnbaumer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Rosenbaum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda M.","family":"Graham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jvssci.2020.07.002_bib1","first-page":"339","article-title":"Endothelial regeneration in the rat carotid artery and the significance of endothelial denudation in the pathogenesis of myointimal thickening","volume":"32","author":"Fishman","year":"1975","journal-title":"Lab Invest"},{"key":"10.1016\/j.jvssci.2020.07.002_bib2","first-page":"407","article-title":"Endothelial regeneration. II. Restitution of endothelial continuity","volume":"41","author":"Haudenschild","year":"1979","journal-title":"Lab Invest"},{"issue":"Suppl","key":"10.1016\/j.jvssci.2020.07.002_bib3","first-page":"47","article-title":"A\u00a0cascade model for restenosis: a special case of atherosclerosis progression","volume":"86","author":"Libby","year":"1992","journal-title":"Circulation"},{"key":"10.1016\/j.jvssci.2020.07.002_bib4","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1093\/cvr\/cvs276","article-title":"Mechanisms of post-intervention arterial remodelling","volume":"96","author":"Goel","year":"2012","journal-title":"Cardiovasc Res"},{"key":"10.1016\/j.jvssci.2020.07.002_bib5","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/bs.apha.2016.08.002","article-title":"Calcium channels in vascular smooth muscle","volume":"78","author":"Ghosh","year":"2017","journal-title":"Adv Pharmacol"},{"key":"10.1016\/j.jvssci.2020.07.002_bib6","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1152\/physrev.1995.75.3.487","article-title":"Regulation of differentiation of vascular smooth muscle cells","volume":"75","author":"Owens","year":"1995","journal-title":"Physiol Rev"},{"key":"10.1016\/j.jvssci.2020.07.002_bib7","doi-asserted-by":"crossref","first-page":"2508","DOI":"10.1161\/ATVBAHA.115.305044","article-title":"Epigenetic control of smooth muscle cell identity and lineage memory","volume":"35","author":"Gomez","year":"2015","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"10.1016\/j.jvssci.2020.07.002_bib8","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1042\/BST0350890","article-title":"Ion channel switching and activation in smooth-muscle cells of occlusive vascular diseases","volume":"35","author":"Beech","year":"2007","journal-title":"Biochem Soc Trans"},{"key":"10.1016\/j.jvssci.2020.07.002_bib9","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1038\/s41422-018-0038-2","article-title":"Structure of the receptor-activated human TRPC6 and TRPC3 ion channels","volume":"28","author":"Tang","year":"2018","journal-title":"Cell Res"},{"key":"10.1016\/j.jvssci.2020.07.002_bib10","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/s00210-005-1052-8","article-title":"Functional characterization and physiological relevance of the TRPC3\/6\/7 subfamily of cation channels","volume":"371","author":"Dietrich","year":"2005","journal-title":"Naunyn Schmiedebergs Arch Pharmacol"},{"key":"10.1016\/j.jvssci.2020.07.002_bib11","doi-asserted-by":"crossref","first-page":"3203","DOI":"10.1091\/mbc.e07-08-0765","article-title":"Elucidation of a TRPC6-TRPC5 channel cascade that restricts endothelial cell movement","volume":"19","author":"Chaudhuri","year":"2008","journal-title":"Mol Biol Cell"},{"key":"10.1016\/j.jvssci.2020.07.002_bib12","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1016\/j.jvs.2014.04.033","article-title":"Hypercholesterolemia inhibits reendothelialization of arterial injuries by TRPC channel activation","volume":"62","author":"Rosenbaum","year":"2015","journal-title":"J\u00a0Vasc Surg"},{"key":"10.1016\/j.jvssci.2020.07.002_bib13","doi-asserted-by":"crossref","first-page":"6980","DOI":"10.1128\/MCB.25.16.6980-6989.2005","article-title":"Increased vascular smooth muscle contractility in TRPC6-\/- mice","volume":"25","author":"Dietrich","year":"2005","journal-title":"Mol Cell Biol"},{"key":"10.1016\/j.jvssci.2020.07.002_bib14","doi-asserted-by":"crossref","first-page":"e96801","DOI":"10.1371\/journal.pone.0096801","article-title":"IHC Profiler: an open source plugin for the quantitative evaluation and automated scoring of immunohistochemistry images of human tissue samples","volume":"9","author":"Varghese","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.jvssci.2020.07.002_bib15","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1161\/01.RES.73.5.792","article-title":"Mouse model of arterial injury","volume":"73","author":"Lindner","year":"1993","journal-title":"Circ Res"},{"key":"10.1016\/j.jvssci.2020.07.002_bib16","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1007\/s12265-015-9616-6","article-title":"Guidelines for the isolation and characterization of murine vascular smooth muscle cells. A report from the International Society of Cardiovascular Translational Research","volume":"8","author":"Adhikari","year":"2015","journal-title":"J\u00a0Cardiovasc Transl Res"},{"key":"10.1016\/j.jvssci.2020.07.002_bib17","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/978-1-61779-207-6_2","article-title":"Scratch-wound assay","volume":"769","author":"Cory","year":"2011","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.jvssci.2020.07.002_bib18","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.1073\/pnas.1600371113","article-title":"Membrane translocation of TRPC6 channels and endothelial migration are regulated by calmodulin and PI3 kinase activation","volume":"113","author":"Chaudhuri","year":"2016","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jvssci.2020.07.002_bib19","doi-asserted-by":"crossref","first-page":"C541","DOI":"10.1152\/ajpcell.00028.2017","article-title":"Integration of TRPC6 and NADPH oxidase activation in lysophosphatidylcholine-induced TRPC5 externalization","volume":"313","author":"Chaudhuri","year":"2017","journal-title":"Am J Physiol Cell Physiol"},{"key":"10.1016\/j.jvssci.2020.07.002_bib20","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat Protoc"},{"key":"10.1016\/j.jvssci.2020.07.002_bib21","doi-asserted-by":"crossref","first-page":"R1529","DOI":"10.1152\/ajpregu.90572.2008","article-title":"Vascular circadian rhythms in a mouse vascular smooth muscle cell line (Movas-1)","volume":"295","author":"Chalmers","year":"2008","journal-title":"Am J Physiol Regul Integr Comp Physiol"},{"key":"10.1016\/j.jvssci.2020.07.002_bib22","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1002\/jcp.25208","article-title":"Smooth muscle cell differentiation: model systems, regulatory mechanisms, and vascular diseases","volume":"231","author":"Shi","year":"2016","journal-title":"J\u00a0Cell Physiol"},{"key":"10.1016\/j.jvssci.2020.07.002_bib23","doi-asserted-by":"crossref","first-page":"4447","DOI":"10.1073\/pnas.1420363112","article-title":"Myocardin is required for maintenance of vascular and visceral smooth muscle homeostasis during postnatal development","volume":"112","author":"Huang","year":"2015","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jvssci.2020.07.002_bib24","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1161\/01.RES.0000138582.36921.9e","article-title":"L-type voltage-gated Ca2+ channels modulate expression of smooth muscle differentiation marker genes via a rho kinase\/myocardin\/SRF-dependent mechanism","volume":"95","author":"Wamhoff","year":"2004","journal-title":"Circ Res"},{"key":"10.1016\/j.jvssci.2020.07.002_bib25","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.devcel.2012.08.017","article-title":"A\u00a0TRPC6-dependent pathway for myofibroblast transdifferentiation and wound healing in\u00a0vivo","volume":"23","author":"Davis","year":"2012","journal-title":"Dev Cell"},{"key":"10.1016\/j.jvssci.2020.07.002_bib26","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1097\/MIB.0000000000000295","article-title":"Intestinal myofibroblast TRPC6 channel may contribute to stenotic fibrosis in Crohn's disease","volume":"21","author":"Kurahara","year":"2015","journal-title":"Inflamm Bowel Dis"},{"key":"10.1016\/j.jvssci.2020.07.002_bib27","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1016\/j.bbadis.2016.12.002","article-title":"Classical transient receptor potential 6 (TRPC6) channels support myofibroblast differentiation and development of experimental pulmonary fibrosis","volume":"1863","author":"Hofmann","year":"2017","journal-title":"Biochim Biophys Acta Mol Basis Dis"},{"key":"10.1016\/j.jvssci.2020.07.002_bib28","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1113\/jphysiol.2011.222232","article-title":"Ca2+\/calmodulin-dependent protein kinase II function in vascular remodelling","volume":"590","author":"Singer","year":"2012","journal-title":"J\u00a0Physiol"},{"key":"10.1016\/j.jvssci.2020.07.002_bib29","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1042\/BJ20120152","article-title":"MEF2 is regulated by CaMKIIdelta2 and a HDAC4-HDAC5 heterodimer in vascular smooth muscle cells","volume":"444","author":"Ginnan","year":"2012","journal-title":"Biochem J"},{"key":"10.1016\/j.jvssci.2020.07.002_bib30","doi-asserted-by":"crossref","first-page":"8361","DOI":"10.1074\/jbc.M111.286203","article-title":"A\u00a0novel RhoA\/ROCK-CPI-17-MEF2C signaling pathway regulates vascular smooth muscle cell gene expression","volume":"287","author":"Pagiatakis","year":"2012","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.jvssci.2020.07.002_bib31","doi-asserted-by":"crossref","first-page":"9785","DOI":"10.1096\/fj.201802811R","article-title":"TRPC6 regulates phenotypic switching of vascular smooth muscle cells through plasma membrane potential-dependent coupling with PTEN","volume":"33","author":"Numaga-Tomita","year":"2019","journal-title":"FASEB J"},{"key":"10.1016\/j.jvssci.2020.07.002_bib32","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/s11357-009-9126-z","article-title":"Expression levels of TRPC1 and TRPC6 ion channels are reciprocally altered in aging rat aorta: implications for age-related vasospastic disorders","volume":"32","author":"Erac","year":"2010","journal-title":"Age (Dordr)"},{"key":"10.1016\/j.jvssci.2020.07.002_bib33","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1007\/BF02623563","article-title":"Human arterial smooth muscle cells in culture: inverse relationship between proliferation and expression of contractile proteins","volume":"25","author":"Fager","year":"1989","journal-title":"Vitro Cell Dev Biol"},{"issue":"Suppl","key":"10.1016\/j.jvssci.2020.07.002_bib34","first-page":"68","article-title":"Experimental models that mimic the differentiation and dedifferentiation of vascular cells","volume":"86","author":"Pauly","year":"1992","journal-title":"Circulation"},{"key":"10.1016\/j.jvssci.2020.07.002_bib35","doi-asserted-by":"crossref","first-page":"C316","DOI":"10.1152\/ajpcell.00125.2002","article-title":"PDGF stimulates pulmonary vascular smooth muscle cell proliferation by upregulating TRPC6 expression","volume":"284","author":"Yu","year":"2003","journal-title":"Am J Physiol Cell Physiol"},{"key":"10.1016\/j.jvssci.2020.07.002_bib36","doi-asserted-by":"crossref","first-page":"C872","DOI":"10.1152\/ajpcell.00334.2004","article-title":"Plasticity of TRPC expression in arterial smooth muscle: correlation with store-operated Ca2+ entry","volume":"288","author":"Bergdahl","year":"2005","journal-title":"Am J Physiol Cell Physiol"},{"key":"10.1016\/j.jvssci.2020.07.002_bib37","doi-asserted-by":"crossref","first-page":"5075","DOI":"10.1038\/s41598-017-04747-w","article-title":"Homer binds to Orai1 and TRPC channels in the neointima and regulates vascular smooth muscle cell migration and proliferation","volume":"7","author":"Jia","year":"2017","journal-title":"Sci Rep"},{"key":"10.1016\/j.jvssci.2020.07.002_bib38","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1084\/jem.20150353","article-title":"TRPC6 is the endothelial calcium channel that regulates leukocyte transendothelial migration during the inflammatory response","volume":"212","author":"Weber","year":"2015","journal-title":"J\u00a0Exp Med"},{"key":"10.1016\/j.jvssci.2020.07.002_bib39","doi-asserted-by":"crossref","first-page":"e0125764","DOI":"10.1371\/journal.pone.0125764","article-title":"A\u00a0critical role for the transient receptor potential channel type 6 in human platelet activation","volume":"10","author":"Vemana","year":"2015","journal-title":"PLoS One"}],"container-title":["JVS-Vascular Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666350320300134?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666350320300134?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T21:13:15Z","timestamp":1762981995000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666350320300134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"references-count":39,"alternative-id":["S2666350320300134"],"URL":"https:\/\/doi.org\/10.1016\/j.jvssci.2020.07.002","relation":{},"ISSN":["2666-3503"],"issn-type":[{"value":"2666-3503","type":"print"}],"subject":[],"published":{"date-parts":[[2020]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Canonical transient receptor potential 6 channel deficiency promotes smooth muscle cells dedifferentiation and increased proliferation after arterial injury","name":"articletitle","label":"Article Title"},{"value":"JVS-Vascular Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jvssci.2020.07.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 by the Society for Vascular Surgery. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}