{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T01:22:59Z","timestamp":1771550579308,"version":"3.50.1"},"reference-count":13,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2011,11,25]],"date-time":"2011-11-25T00:00:00Z","timestamp":1322179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["NSC\u201097\u20102320\u2010B\u2010037\u2010006\u2010MY3"],"award-info":[{"award-number":["NSC\u201097\u20102320\u2010B\u2010037\u2010006\u2010MY3"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["VGHKS96\u2010033"],"award-info":[{"award-number":["VGHKS96\u2010033"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["VGHKS97\u2010044"],"award-info":[{"award-number":["VGHKS97\u2010044"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>This study investigated whether KMUP\u20101, a xanthine\u2010based derivative, inhibits L\u2010type Ca<jats:sup>2+<\/jats:sup> currents (I<jats:sub>Ca,L<\/jats:sub>) in rat basilar artery smooth muscle cells (RBASMCs). We used whole cell patch\u2010clamp recording to monitor Ba<jats:sup>2+<\/jats:sup> currents (I<jats:sub>Ba<\/jats:sub>) through L\u2010type Ca<jats:sup>2+<\/jats:sup> channels (LTCCs). Under voltage\u2013clamp conditions, holding at \u201340 mV, KMUP\u20101 (1, 3, 10 \u03bcM) inhibited I<jats:sub>Ba<\/jats:sub> in a concentration\u2010dependent manner and its IC<jats:sub>50<\/jats:sub> value was 2.27 \u00b1 0.45 \u03bcM. A high concentration of KMUP\u20101 (10 \u03bcM) showed without modifying the I<jats:sub>Ba<\/jats:sub> current\u2013voltage relationship. On the other hand, the protein kinase C (PKC) activator phorbol 12\u2010myristate 13\u2010acetate (PMA, 1\u2009\u03bcM) increase I<jats:sub>Ba<\/jats:sub> was inhibited by KMUP\u20101. Pretreatment with the PKC inhibitor chelerythrine (5 \u03bcM) intensified KMUP\u20101\u2010inhibited I<jats:sub>Ba<\/jats:sub>. However, the Rho kinase inhibitor Y\u201027632 (30 \u03bcM) failed to affect the I<jats:sub>Ba<\/jats:sub> inhibition by KMUP\u20101. In light of these results, we suggest that KMUP\u20101 inhibition of LTCCs in concentration\u2010 and voltage\u2010dependent manners in RBASMCs may be due, at least in part, to its modulation of the PKC pathway.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.kjms.2011.10.026","type":"journal-article","created":{"date-parts":[[2011,12,2]],"date-time":"2011-12-02T09:25:04Z","timestamp":1322817904000},"page":"538-543","source":"Crossref","is-referenced-by-count":7,"title":["KMUP\u20101 inhibits L\u2010type Ca<sup>2+<\/sup> channels involved the protein kinase C in rat basilar artery myocytes"],"prefix":"10.1002","volume":"27","author":[{"given":"Jun\u2010Yih","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min\u2010Chi","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Wen","family":"Chu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su\u2010Ling","family":"Hsieh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ing\u2010Jun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin\u2010Nan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,11,25]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704231"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704554"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705791"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706387"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00605.2002"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703360"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000138582.36921.9e"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050237"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500360102"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00926.2006"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704468"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.35.1.173"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2009.03.001"}],"container-title":["The Kaohsiung Journal of Medical Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1607551X1100249X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1607551X1100249X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.kjms.2011.10.026","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1016\/j.kjms.2011.10.026","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.kjms.2011.10.026","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T08:41:19Z","timestamp":1694680879000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1016\/j.kjms.2011.10.026"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11,25]]},"references-count":13,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["10.1016\/j.kjms.2011.10.026"],"URL":"https:\/\/doi.org\/10.1016\/j.kjms.2011.10.026","archive":["Portico"],"relation":{},"ISSN":["1607-551X","2410-8650"],"issn-type":[{"value":"1607-551X","type":"print"},{"value":"2410-8650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11,25]]}}}