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To investigate the correlation between laminin 332 g2 distribution and patient outcome, we performed a semiquantitative immunohistochemical analysis of 35 paraffin\u2010embedded samples using the antibody D4B5, which is specific for the laminin 5 \u03b32 chain. To evaluate the role of laminin 332 in NBT\u2010II cell motility and invasion, we used a scratch assay and the Boyden chamber chemoinvasion system. Tumor stage and grade were significantly correlated with a loss of laminin 332 \u03b32 chain from the basement membrane (<jats:italic>p<\/jats:italic>\u2009=\u20090.001) and its retention in the cytoplasm (<jats:italic>p<\/jats:italic>\u2009=\u20090.001) (Kruskal\u2013Wallis test). Kaplan\u2013Meier survival curves revealed an association between the risk of progression and cytoplasmic retention of the laminin 332 <jats:bold>\u03b3<\/jats:bold>2 chain. In addition, an <jats:italic>in\u2009vitro<\/jats:italic> scratch assay showed an increase in the migration of cells treated with laminin 332 from their cluster. The Boyden chamber assay showed that laminin 332 potentiated NBT\u2010II cell invasion. Immunohistochemistry results showed that bladder cancer patients with a higher malignancy expressed more laminin 332. The <jats:italic>in\u2009vitro<\/jats:italic> scratch and invasion assay showed that laminin 332 stimulated the motility and invasion of bladder cancer cells. The invasion assay explains the correlation between laminin 332 expression and bladder cancer malignancy.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.kjms.2012.12.003","type":"journal-article","created":{"date-parts":[[2013,2,14]],"date-time":"2013-02-14T17:46:20Z","timestamp":1360863980000},"page":"422-429","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":19,"title":["Effect of laminin 332 on motility and invasion in bladder 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