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Mechanical stimulus on detrusor tissue is critical to BOO disease progression. Our previous studies confirm that mechanical stimulus triggers human bladder smooth muscle cell (HBSMC) proliferation. To better understand the signal transduction mechanisms for this process we detected cell cycle machinery of HBSMC (Bose \u00ae Biodynamic, Minnetonka, MN, USA). HBSMCs cultured in scaffolds were subjected to four different pressures (0\u2009cmH<jats:sub>2<\/jats:sub>O, 100\u2009cmH<jats:sub>2<\/jats:sub>O, 200\u2009cmH<jats:sub>2<\/jats:sub>O, and 300\u2009cmH<jats:sub>2<\/jats:sub>O) for 24\u2009hours, which were controlled by a BOSE BioDynamic bioreactor. Then we used flow cytometry to examine cell cycle distribution, polymerase chain reaction, and immunoblotting to quantify Skp2, p27, and p21 expression in each group. Additionally, Skp2 was silenced in HBSMCs using small interfering RNA to validate the role of Skp2 in mediating pressure\u2010induced cell cycle progression. Compared with the 0\u2009cmH<jats:sub>2<\/jats:sub>O control, HBSMCs in the 200\u2009cmH<jats:sub>2<\/jats:sub>O and 300\u2009cmH<jats:sub>2<\/jats:sub>O groups exhibited high\u2010level expression of Skp2 gene and low\u2010level expression of p27 protein. However, p21, another downstream signal of Skp2, showed no significant change between groups. In addition, Skp2 silencing abolished increases in cell proliferation induced by pressure. To the best of our knowledge, this is the first report on the functional importance of Skp2 in cyclic hydrodynamic pressure stimulated HBSMC proliferation. The signal transduction mechanism for this process involves p27 as well as p21 signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.kjms.2013.07.002","type":"journal-article","created":{"date-parts":[[2013,9,5]],"date-time":"2013-09-05T05:30:24Z","timestamp":1378359024000},"page":"181-186","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"title":["Skp2\u2010mediated degradation of p27 regulates cell cycle progression in compressed human bladder smooth muscle 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muscle cell hypertrophy and hyperplasia in the rat detrusor after short\u2010time infravesical outflow","volume":"131","author":"Uvelius B.","year":"1984","journal-title":"J Urol"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)35135-2"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-008-0268-x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2009.08.050"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)68136-0"},{"key":"e_1_2_6_10_2","first-page":"691","article-title":"Effects of physiological range hydrostatic pressure on bladder smooth muscle cells in\u2009vitro","volume":"29","author":"Wei T.","year":"2012","journal-title":"Sheng Wu Yi Xue Gong Cheng Xue Za 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