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This herb exhibits multifunctional activities, including anticancer, anti\u2010inflammation, and antioxidant effects. This paper investigated the <jats:italic>in\u2009vitro<\/jats:italic> and <jats:italic>in\u2009vivo<\/jats:italic> anti\u2010inflammatory effect of <jats:italic>A. trifoliatus<\/jats:italic>. High\u2010performance liquid chromatography analysis established the fingerprint chromatogram of the ethyl acetate fraction of <jats:italic>A. trifoliatus<\/jats:italic> (EAAT). The anti\u2010inflammatory effect of EAAT was detected using lipopolysaccharide (LPS) stimulation of the mouse macrophage cell line RAW264.7 <jats:italic>in\u2009vitro<\/jats:italic> and LPS\u2010induced lung injury <jats:italic>in\u2009vivo<\/jats:italic>. The effects of EAAT on LPS\u2010induced production of inflammatory mediators in RAW264.7 murine macrophages and the mouse model were measured using enzyme\u2010linked immunosorbent assay and Western blot. EAAT attenuated the production of LPS\u2010induced nitric oxide (NO), tumor necrosis factor\u2010alpha, interleukin\u20101\u03b2 (IL\u20101\u03b2), and IL\u20106 <jats:italic>in\u2009vitro<\/jats:italic> and <jats:italic>in\u2009vivo<\/jats:italic>. Pretreatment with EAAT markedly reduced LPS\u2010induced histological alterations in lung tissues. Furthermore, EAAT significantly reduced the number of total cells and protein concentration levels in the bronchoalveolar lavage fluid. Western blotting test results revealed that EAAT blocked protein expression of inducible NO synthase, cyclooxygenase\u20102, phosphorylation of Nuclear factor\u2010kappa\u2010B Inhibitor alpha (I\u03baB\u2010\u03b1) protein, and mitogen\u2010activated protein kinases in LPS\u2010stimulated RAW264.7 cells as well as LPS\u2010induced lung injury. This study suggests that <jats:italic>A. trifoliatus<\/jats:italic> may be a potential therapeutic candidate for the treatment of inflammatory diseases.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.kjms.2015.07.007","type":"journal-article","created":{"date-parts":[[2015,9,12]],"date-time":"2015-09-12T03:18:11Z","timestamp":1442027891000},"page":"499-509","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"title":["<i>Acanthopanax trifoliatus<\/i> inhibits lipopolysaccharide\u2010induced inflammatory response <i>in\u2009vitro<\/i> and <i>in\u2009vivo<\/i>"],"prefix":"10.1002","volume":"31","author":[{"given":"Tzu\u2010Mei","family":"Chien","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Po\u2010Chow","family":"Hsieh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyh\u2010Shyun","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeng\u2010Shyan","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Ling","family":"Ho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan\u2010Shiun","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guan\u2010Jhong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,9,10]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.11.005"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1016\/j.jep.2011.07.041","article-title":"Antioxidant, 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