{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T11:25:17Z","timestamp":1774437917779,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,9,30]],"date-time":"2016-09-30T00:00:00Z","timestamp":1475193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2016,9,30]],"date-time":"2016-09-30T00:00:00Z","timestamp":1475193600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,8,25]],"date-time":"2016-08-25T00:00:00Z","timestamp":1472083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Health Research Institute, Infectious and Tropical Diseases Research Center, Jundishapur University of Medical Sciences","award":["92133"],"award-info":[{"award-number":["92133"]}]}],"content-domain":{"domain":["kjms-online.com","clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Meningitis is a life\u2010threatening infection associated with a high mortality and morbidity worldwide. <jats:italic>Neisseria meningitidis<\/jats:italic>, <jats:italic>Haemophilus influenzae<\/jats:italic>, and <jats:italic>Streptococcus pneumoniae<\/jats:italic> are the most prevalent infectious agents that cause bacterial meningitis (BM). The objective of this study was to determine the frequencies of these three bacteria using bacterial cultures and polymerase chain reaction (PCR). In our cross\u2010sectional study, cerebrospinal fluid (CSF) specimens were obtained from 196 patients who were suspected of having BM and referred to the pediatric ward of Abuzar Hospital (Ahvaz, Iran). The samples were monitored by gram stain, cultures, and the PCR method. The patients' age mean was 23\u2009\u00b1\u20090.56\u2009months. The 196 patients comprised 92 (46.9%) boys and 104 (53.06%) girls. Based on bacterial cultures, just three isolates of <jats:italic>H. influenzae<\/jats:italic> were detected. However, PCR detected this bacterium in eight patients. <jats:italic>Streptococcus pneumoniae<\/jats:italic> was detected in five (2.5%) patients by the amplification of the <jats:italic>lytA<\/jats:italic> gene and in one (0.5%) patient by ply. In this study, no <jats:italic>N. meningitidis<\/jats:italic> isolate was in the CSF samples, based on the bacterial culture or PCR results. <jats:italic>Streptococcus agalactiae<\/jats:italic> was detected only in one patient, based on PCR. In conclusion, in the present study, the PCR method was more sensitive and rapid than culture for detecting the infectious agents in BM. For this reason, this diagnosis method is recommended for BM.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.kjms.2016.08.009","type":"journal-article","created":{"date-parts":[[2016,10,2]],"date-time":"2016-10-02T04:51:04Z","timestamp":1475383864000},"page":"501-506","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"title":["Detection of <i>Haemophilus influenzae type b, Streptococcus agalactiae, Streptococcus pneumoniae<\/i> and <i>Neisseria meningitidis<\/i> in\u2009CSF\u2009specimens of children suspicious of\u2009Meningitis in Ahvaz, Iran"],"prefix":"10.1002","volume":"32","author":[{"given":"Mansour","family":"Amin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mozhgan","family":"Ghaderpanah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tahereh","family":"Navidifar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,30]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13693-8"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(09)70306-8"},{"key":"e_1_2_7_4_2","first-page":"53","article-title":"Risk factors for community\u2010acquired bacterial meningitis in adults","volume":"73","author":"Adriani K.S.","year":"2015","journal-title":"Neth J Med"},{"key":"e_1_2_7_5_2","doi-asserted-by":"crossref","first-page":"380283","DOI":"10.5402\/2011\/380283","article-title":"The use of dexamethasone in bacterial meningitis in children and adults: a retrospective analysis","volume":"2011","author":"Cornelis A.S.","year":"2011","journal-title":"ISRN Pediatr"},{"key":"e_1_2_7_6_2","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.12669\/pjms.295.3902","article-title":"Identification of etiological agents by LPA and PCR in childhood meningitis","volume":"29","author":"Khan M.","year":"2013","journal-title":"Pak J Med Sci"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-13-26"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12887-015-0416-6"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1179\/027249303225007752"},{"key":"e_1_2_7_10_2","doi-asserted-by":"crossref","first-page":"2706","DOI":"10.4161\/hv.29678","article-title":"Meningitis caused by Neisseria meningitidis, Hemophilus influenzae type B and Streptococcus pneumoniae during 2005\u20132012 in Turkey. 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