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Accidental infection in humans causes the gastrointestinal pathophysiological effects of mechanical tissue damage by migrating larvae. The mechanism of the infective larval invasion and migration is suspected to involve larval excretory\/secretory proteases and motility. This study demonstrates the penetration rate of the infective larvae of <jats:italic>Anisakis pegreffii<\/jats:italic> in mouse gastrointestine depends on the time after infection, and that only 15% of larvae remain in the gastrointestinal tract 3\u2009h after infection. Strong activities of matrix metalloproteinases (MMPs) and serine proteases, especially plasmin, were found in the excretory\/secretory products of <jats:italic>A. pegreffii<\/jats:italic>; these can be inhibited by ONO\u20104817 and phenylmethylsulfonyl fluoride, respectively. The protease activity was also significantly decreased in another 1\u2009h of cultivation of larvae in fresh 0.9% normal saline (NS) after previous cultivation for 48\u2009h in NS. The motility scores of larvae were significantly lower after 48\u2009h of cultivation in NS. The penetration rate of <jats:italic>A. pegreffii<\/jats:italic> larvae in the gastrointestine of infected mice sequentially were 90% in the freshly prepared, 68% in serine protease inhibited, 55% in MMPs inhibited larvae, and 16% in larvae cultivated in NS for 48\u2009h. Therefore, this study demonstrates that MMPs and serine proteases excreted and secreted by <jats:italic>A. pegreffii<\/jats:italic> and the mechanical movement of infective larvae participate in the penetration of the gastrointestine of mice after infection.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.kjms.2017.08.002","type":"journal-article","created":{"date-parts":[[2017,8,24]],"date-time":"2017-08-24T01:46:25Z","timestamp":1503539185000},"page":"594-601","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["Excretory\/secretory proteases and mechanical movement of <i>Anisakis pegreffii<\/i> infective larvae in the penetration of BALB\/c mice 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