{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T01:49:12Z","timestamp":1785894552452,"version":"3.56.0"},"reference-count":26,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004417","name":"Global Fund to Fight AIDS Tuberculosis and Malaria","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004417","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI173171"],"award-info":[{"award-number":["R01AI173171"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI175134"],"award-info":[{"award-number":["R01AI175134"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R61AI187100"],"award-info":[{"award-number":["R61AI187100"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1209843"],"award-info":[{"award-number":["OPP1209843"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["INV-008263"],"award-info":[{"award-number":["INV-008263"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["LPDR-20864-007-18"],"award-info":[{"award-number":["LPDR-20864-007-18"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","thelancet.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Lancet Microbe"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.lanmic.2025.101324","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T18:57:50Z","timestamp":1779217070000},"page":"101324","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Artemether\u2013lumefantrine for the treatment of Plasmodium falciparum malaria in Laos: a therapeutic efficacy study coupled with genomic and in vitro phenotypic analyses"],"prefix":"10.1016","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1578-455X","authenticated-orcid":false,"given":"Lionel B","family":"Feufack-Donfack","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nimol","family":"Khim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6182-4010","authenticated-orcid":false,"given":"Baura","family":"Tat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virasack","family":"Banouvong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boualam","family":"Khamlome","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keobouphaphone","family":"Chindavongsal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chitsavang","family":"Chanthavisouk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Reyburn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Shortus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nimol","family":"Kloeung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sopheakvatey","family":"Ke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanra","family":"Khean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandavin","family":"Seng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanvong","family":"Kul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rotha","family":"Eam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnes","family":"Orban","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Salvador","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myriam","family":"Vezain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre-Emilien","family":"Dorgebray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romain","family":"Copp\u00e9e","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederic","family":"Ariey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Witkowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Ringwald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3135-1175","authenticated-orcid":false,"given":"Jean","family":"Popovici","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.lanmic.2025.101324_bib2","doi-asserted-by":"crossref","DOI":"10.1056\/NEJMoa0808859","article-title":"Artemisinin resistance in Plasmodium falciparum malaria","volume":"361","author":"Dondorp","year":"2009","journal-title":"N Engl J Med"},{"key":"10.1016\/j.lanmic.2025.101324_bib3","doi-asserted-by":"crossref","DOI":"10.1093\/infdis\/jiu491","article-title":"Independent emergence of artemisinin resistance mutations among Plasmodium falciparum in southeast Asia","volume":"211","author":"Takala-Harrison","year":"2015","journal-title":"J Infect Dis"},{"key":"10.1016\/j.lanmic.2025.101324_bib4","doi-asserted-by":"crossref","DOI":"10.1038\/s41591-020-1005-2","article-title":"Emergence and clonal expansion of in vitro artemisinin-resistant Plasmodium falciparum kelch13 R561H mutant parasites in Rwanda","volume":"26","author":"Uwimana","year":"2020","journal-title":"Nat Med"},{"key":"10.1016\/j.lanmic.2025.101324_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/S1473-3099(25)00013-1","article-title":"Impact of the Global Fund Regional Artemisinin-resistance Initiative on malaria control and elimination in the Greater Mekong subregion of southeast Asia","volume":"25","author":"Pley","year":"2025","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.lanmic.2025.101324_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/S1473-3099(19)30391-3","article-title":"Determinants of dihydroartemisinin-piperaquine treatment failure in Plasmodium falciparum malaria in Cambodia, Thailand, and Vietnam: a prospective clinical, pharmacological, and genetic study","volume":"19","author":"van der Pluijm","year":"2019","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.lanmic.2025.101324_bib11","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1186\/1475-2875-11-184","article-title":"Efficacy of artemether-lumefantrine, the nationally-recommended artemisinin combination for the treatment of uncomplicated falciparum malaria, in southern Laos","volume":"11","author":"Mayxay","year":"2012","journal-title":"Malar J"},{"key":"10.1016\/j.lanmic.2025.101324_bib12","doi-asserted-by":"crossref","DOI":"10.1086\/424512","article-title":"Randomized comparison of chloroquine plus sulfadoxine-pyrimethamine versus artesunate plus mefloquine versus artemether-lumefantrine in the treatment of uncomplicated falciparum malaria in the Lao People\u2019s Democratic Republic","volume":"39","author":"Mayxay","year":"2004","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.lanmic.2025.101324_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/S1473-3099(22)00697-1","article-title":"Malaria outbreak in Laos driven by a selective sweep for Plasmodium falciparum kelch13 R539T mutants: a genetic epidemiology analysis","volume":"23","author":"Wasakul","year":"2023","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.lanmic.2025.101324_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/S1473-3099(13)70252-4","article-title":"Novel phenotypic assays for the detection of artemisinin-resistant Plasmodium falciparum malaria in Cambodia: in-vitro and ex-vivo drug-response studies","volume":"13","author":"Witkowski","year":"2013","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.lanmic.2025.101324_bib18","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.02975-15","article-title":"The redox cycler Plasmodione is a fast-acting antimalarial lead compound with pronounced activity against sexual and early asexual blood-stage parasites","volume":"60","author":"Ehrhardt","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.lanmic.2025.101324_bib19","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.48.5.1803-1806.2004","article-title":"Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening","volume":"48","author":"Smilkstein","year":"2004","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.lanmic.2025.101324_bib20","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/nature12876","article-title":"A molecular marker of artemisinin-resistant Plasmodium falciparum malaria","volume":"505","author":"Ariey","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.lanmic.2025.101324_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/S1473-3099(16)30415-7","article-title":"A surrogate marker of piperaquine-resistant Plasmodium falciparum malaria: a phenotype\u2013genotype association study","volume":"17","author":"Witkowski","year":"2017","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.lanmic.2025.101324_bib22","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1186\/s12936-016-1641-7","article-title":"Whole genome sequencing of Plasmodium falciparum from dried blood spots using selective whole genome amplification","volume":"15","author":"Oyola","year":"2016","journal-title":"Malar J"},{"key":"10.1016\/j.lanmic.2025.101324_bib23","doi-asserted-by":"crossref","DOI":"10.1093\/bioinformatics\/btp324","article-title":"Fast and accurate short read alignment with Burrows\u2013Wheeler transform","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.lanmic.2025.101324_bib24","doi-asserted-by":"crossref","DOI":"10.1093\/gigascience\/giab008","article-title":"Twelve years of SAMtools and BCFtools","volume":"10","author":"Danecek","year":"2021","journal-title":"GigaScience"},{"key":"10.1016\/j.lanmic.2025.101324_bib25","doi-asserted-by":"crossref","DOI":"10.1093\/bioinformatics\/btv566","article-title":"Qualimap 2: advanced multi-sample quality control for high-throughput sequencing data","volume":"32","author":"Okonechnikov","year":"2016","journal-title":"Bioinformatics"},{"key":"10.1016\/j.lanmic.2025.101324_bib26","doi-asserted-by":"crossref","DOI":"10.1093\/cid\/ciac813","article-title":"Nosocomial malaria transmissions resolved by genomic analyses\u2014a retrospective case report study in France: 2007\u20132021","volume":"76","author":"Copp\u00e9e","year":"2023","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.lanmic.2025.101324_bib27","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.00968-08","article-title":"Selection of Plasmodium falciparum multidrug resistance gene 1 alleles in asexual stages and gametocytes by artemether-lumefantrine in Nigerian children with uncomplicated falciparum malaria","volume":"53","author":"Happi","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.lanmic.2025.101324_bib28","doi-asserted-by":"crossref","DOI":"10.1093\/infdis\/jis747","article-title":"Plasmodium falciparum drug resistance phenotype as assessed by patient antimalarial drug levels and its association with pfmdr1 polymorphisms","volume":"207","author":"Malmberg","year":"2013","journal-title":"J Infect Dis"},{"key":"10.1016\/j.lanmic.2025.101324_bib29","doi-asserted-by":"crossref","first-page":"6353","DOI":"10.1038\/s41467-022-33873-x","article-title":"Decreased susceptibility of Plasmodium falciparum to both dihydroartemisinin and lumefantrine in northern Uganda","volume":"13","author":"Tumwebaze","year":"2022","journal-title":"Nat Commun"},{"key":"10.1016\/j.lanmic.2025.101324_bib30","doi-asserted-by":"crossref","DOI":"10.1093\/cid\/ciad724","article-title":"Treatment failure in a UK malaria patient harboring genetically variant Plasmodium falciparum From Uganda with reduced in vitro susceptibility to artemisinin and lumefantrine","volume":"78","author":"van Schalkwyk","year":"2024","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.lanmic.2025.101324_bib31","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1186\/1475-2875-12-450","article-title":"A SYBR Green 1-based in vitro test of susceptibility of Ghanaian Plasmodium falciparum clinical isolates to a panel of anti-malarial drugs","volume":"12","author":"Quashie","year":"2013","journal-title":"Malar J"},{"key":"10.1016\/j.lanmic.2025.101324_bib32","doi-asserted-by":"crossref","DOI":"10.4269\/ajtmh.20-0235","article-title":"Molecular surveillance and in vitro drug sensitivity study of Plasmodium falciparum isolates from the China-Myanmar border","volume":"103","author":"Wang","year":"2020","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.lanmic.2025.101324_bib34","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1186\/s12936-018-2625-6","article-title":"Heterogeneous distribution of k13 mutations in Plasmodium falciparum in Laos","volume":"17","author":"Iwagami","year":"2018","journal-title":"Malar J"},{"key":"10.1016\/j.lanmic.2025.101324_bib35","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/s12936-024-04851-z","article-title":"Progress towards malaria elimination in the Greater Mekong subregion: perspectives from the World Health Organization","volume":"23","author":"Manzoni","year":"2024","journal-title":"Malar J"}],"container-title":["The Lancet Microbe"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666524725002526?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666524725002526?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T01:14:03Z","timestamp":1785892443000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666524725002526"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":26,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["S2666524725002526"],"URL":"https:\/\/doi.org\/10.1016\/j.lanmic.2025.101324","relation":{},"ISSN":["2666-5247"],"issn-type":[{"value":"2666-5247","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Artemether\u2013lumefantrine for the treatment of Plasmodium falciparum malaria in Laos: a therapeutic efficacy study coupled with genomic and in vitro phenotypic analyses","name":"articletitle","label":"Article Title"},{"value":"The Lancet Microbe","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.lanmic.2025.101324","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"101324"}}