{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:35:42Z","timestamp":1792784142515,"version":"4.5.0"},"reference-count":26,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006114","name":"S\u00e4chsisches Staatsministerium f\u00fcr Wissenschaft und Kunst","doi-asserted-by":"publisher","award":["100613388"],"award-info":[{"award-number":["100613388"]}],"id":[{"id":"10.13039\/501100006114","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006114","name":"S\u00e4chsisches Staatsministerium f\u00fcr Wissenschaft und Kunst","doi-asserted-by":"publisher","award":["100257255"],"award-info":[{"award-number":["100257255"]}],"id":[{"id":"10.13039\/501100006114","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001655","name":"German Academic Exchange Service","doi-asserted-by":"publisher","award":["57599539"],"award-info":[{"award-number":["57599539"]}],"id":[{"id":"10.13039\/501100001655","id-type":"DOI","asserted-by":"publisher"},{"id":"https:\/\/ror.org\/039djdh30","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001655","name":"German Academic Exchange Service","doi-asserted-by":"publisher","award":["57417782"],"award-info":[{"award-number":["57417782"]}],"id":[{"id":"10.13039\/501100001655","id-type":"DOI","asserted-by":"publisher"},{"id":"https:\/\/ror.org\/039djdh30","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/100001126","name":"Geisinger Health System Foundation","doi-asserted-by":"publisher","award":["P0134_23_N6"],"award-info":[{"award-number":["P0134_23_N6"]}],"id":[{"id":"10.13039\/100001126","id-type":"DOI","asserted-by":"publisher"},{"id":"https:\/\/ror.org\/02qdbgx97","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002347","name":"Federal Ministry of Education and Research Bonn Office","doi-asserted-by":"publisher","award":["01DQ20002"],"award-info":[{"award-number":["01DQ20002"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"},{"id":"https:\/\/ror.org\/04pz7b180","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","thelancet.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Lancet Microbe"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.lanmic.2026.101390","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T20:09:02Z","timestamp":1789502942000},"page":"101390","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigating the role of a non-synonymous mutation at dhfr codon 50 in maintaining sulphadoxine\u2013pyrimethamine resistance in Plasmodium falciparum: a modelling study"],"prefix":"10.1016","author":[{"given":"Carola J","family":"Salas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nessma","family":"Adil Mahmoud Yousif","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen E","family":"Lizewski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christie A","family":"Joya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo O","family":"Valdivia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J","family":"Bacon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4138-1180","authenticated-orcid":false,"given":"Kristan A","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.lanmic.2026.101390_bib1","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/nyas.12662","article-title":"The molecular basis of antifolate resistance in Plasmodium falciparum: looking beyond point mutations","volume":"1342","author":"Heinberg","year":"2015","journal-title":"Ann N Y Acad Sci"},{"key":"10.1016\/j.lanmic.2026.101390_bib2","first-page":"149","article-title":"Charles Darwin meets Ronald Ross: a population-genetic framework for the evolutionary dynamics of malaria","volume":"vol. 7","author":"Schneider","year":"2021"},{"key":"10.1016\/j.lanmic.2026.101390_bib3","doi-asserted-by":"crossref","first-page":"18883","DOI":"10.1073\/pnas.0911317106","article-title":"Geographic patterns of Plasmodium falciparum drug resistance distinguished by differential responses to amodiaquine and chloroquine","volume":"106","author":"S\u00e1","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.lanmic.2026.101390_bib4","doi-asserted-by":"crossref","first-page":"2787","DOI":"10.1093\/jac\/dkv199","article-title":"Fitness of artemisinin-resistant Plasmodium falciparum in vitro","volume":"70","author":"Hott","year":"2015","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.lanmic.2026.101390_bib5","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1086\/655659","article-title":"Return of chloroquine-susceptible falciparum malaria in Malawi was a reexpansion of diverse susceptible parasites","volume":"202","author":"Laufer","year":"2010","journal-title":"J Infect Dis"},{"issue":"suppl 3","key":"10.1016\/j.lanmic.2026.101390_bib6","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1590\/S0074-02761992000700058","article-title":"Epidemiological distribution of Plasmodium falciparum drug resistance in Brazil and its relevance to the treatment and control of malaria","volume":"87","author":"de Souza","year":"1992","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.lanmic.2026.101390_bib7","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0166-6851(98)00075-9","article-title":"Antifolate resistance due to new and known Plasmodium falciparum dihydrofolate reductase mutations expressed in yeast","volume":"94","author":"Cortese","year":"1998","journal-title":"Mol Biochem Parasitol"},{"key":"10.1016\/j.lanmic.2026.101390_bib8","doi-asserted-by":"crossref","first-page":"1526","DOI":"10.1093\/molbev\/msg162","article-title":"A selective sweep driven by pyrimethamine treatment in Southeast Asian malaria parasites","volume":"20","author":"Nair","year":"2003","journal-title":"Mol Biol Evol"},{"key":"10.1016\/j.lanmic.2026.101390_bib9","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1186\/s12936-020-03454-8","article-title":"Changes in the frequencies of Plasmodium falciparum dhps and dhfr drug-resistant mutations in children from Western Kenya from 2005 to 2018: the rise of Pfdhps S436H","volume":"19","author":"Pacheco","year":"2020","journal-title":"Malar J"},{"key":"10.1016\/j.lanmic.2026.101390_bib10","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.1016\/S0140-6736(98)24022-0","article-title":"Molecular assays for surveillance of antifolate-resistant malaria","volume":"351","author":"Kublin","year":"1998","journal-title":"Lancet"},{"key":"10.1016\/j.lanmic.2026.101390_bib11","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1590\/S0074-02762000000500020","article-title":"Mutations in Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase of isolates from the Amazon region of Brazil","volume":"95","author":"Vasconcelos","year":"2000","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.lanmic.2026.101390_bib12","doi-asserted-by":"crossref","first-page":"457","DOI":"10.4269\/ajtmh.1999.61.457","article-title":"Point mutations in dihydrofolate reductase and dihydropteroate synthase genes of Plasmodium falciparum isolates from Venezuela","volume":"61","author":"Urdaneta","year":"1999","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.lanmic.2026.101390_bib13","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1186\/s12936-022-04403-3","article-title":"Molecular surveillance of anti-malarial drug resistance genes in Plasmodium falciparum isolates in Odisha, India","volume":"21","author":"Rana","year":"2022","journal-title":"Malar J"},{"key":"10.1016\/j.lanmic.2026.101390_bib14","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1086\/504687","article-title":"Antifolate resistance in Plasmodium falciparum: multiple origins and identification of novel dhfr alleles","volume":"194","author":"McCollum","year":"2006","journal-title":"J Infect Dis"},{"key":"10.1016\/j.lanmic.2026.101390_bib15","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1186\/s12936-019-2946-0","article-title":"Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by next generation sequencing of Plasmodium falciparum","volume":"18","author":"Smith-Aguasca","year":"2019","journal-title":"Malar J"},{"key":"10.1016\/j.lanmic.2026.101390_bib16","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1186\/s12936-016-1358-7","article-title":"Low-grade sulfadoxine\u2013pyrimethamine resistance in Plasmodium falciparum parasites from Lubango, Angola","volume":"15","author":"Kaingona-Daniel","year":"2016","journal-title":"Malar J"},{"key":"10.1016\/j.lanmic.2026.101390_bib17","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1186\/s12936-018-2620-y","article-title":"Prevalence of molecular markers of sulfadoxine\u2013pyrimethamine and artemisinin resistance in Plasmodium falciparum from Pakistan","volume":"17","author":"Yaqoob","year":"2018","journal-title":"Malar J"},{"key":"10.1016\/j.lanmic.2026.101390_bib18","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.ijpddr.2013.07.001","article-title":"Polymorphism in dhfr\/dhps genes, parasite density and ex vivo response to pyrimethamine in Plasmodium falciparum malaria parasites in Thies, Senegal","volume":"3","author":"Ndiaye","year":"2013","journal-title":"Int J Parasitol Drugs Drug Resist"},{"key":"10.1016\/j.lanmic.2026.101390_bib19","doi-asserted-by":"crossref","first-page":"12025","DOI":"10.1073\/pnas.0905922106","article-title":"Stepwise acquisition of pyrimethamine resistance in the malaria parasite","volume":"106","author":"Lozovsky","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.lanmic.2026.101390_bib20","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1057\/palgrave.jphp.3190032","article-title":"Modifying national malaria treatment policies in Peru","volume":"25","author":"Ruebush","year":"2004","journal-title":"J Public Health Policy"},{"key":"10.1016\/j.lanmic.2026.101390_bib21","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1006\/meth.1997.0511","article-title":"Yeast as a model system to study drugs effective against apicomplexan proteins","volume":"13","author":"Sibley","year":"1997","journal-title":"Methods"},{"key":"10.1016\/j.lanmic.2026.101390_bib22","article-title":"A maximum-likelihood method to estimate haplotype frequencies and prevalence alongside multiplicity of infection from SNP data","volume":"2","author":"Obama","year":"2022","journal-title":"Front Epidemiol"},{"key":"10.1016\/j.lanmic.2026.101390_bib23","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1475-2875-12-15","article-title":"Fitness components and natural selection: why are there different patterns on the emergence of drug resistance in Plasmodium falciparum and Plasmodium vivax?","volume":"12","author":"Schneider","year":"2013","journal-title":"Malar J"},{"key":"10.1016\/j.lanmic.2026.101390_bib24","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0005674","article-title":"Predominance of asymptomatic and sub-microscopic infections characterizes the Plasmodium gametocyte reservoir in the Peruvian Amazon","volume":"11","author":"Rovira-Vallbona","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.lanmic.2026.101390_bib25","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0166-6851(96)02808-3","article-title":"Analysis in yeast of antimalaria drugs that target the dihydrofolate reductase of Plasmodium falciparum","volume":"85","author":"Wooden","year":"1997","journal-title":"Mol Biochem Parasitol"},{"key":"10.1016\/j.lanmic.2026.101390_bib26","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1186\/1475-2875-9-247","article-title":"Anti-folate drug resistance in Africa: meta-analysis of reported dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) mutant genotype frequencies in African Plasmodium falciparum parasite populations","volume":"9","author":"Sridaran","year":"2010","journal-title":"Malar J"}],"container-title":["The Lancet Microbe"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666524726000455?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666524726000455?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T20:14:52Z","timestamp":1789935292000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666524726000455"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":26,"alternative-id":["S2666524726000455"],"URL":"https:\/\/doi.org\/10.1016\/j.lanmic.2026.101390","relation":{},"ISSN":["2666-5247"],"issn-type":[{"value":"2666-5247","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Investigating the role of a non-synonymous mutation at dhfr codon 50 in maintaining sulphadoxine\u2013pyrimethamine resistance in Plasmodium falciparum: a modelling study","name":"articletitle","label":"Article Title"},{"value":"The Lancet Microbe","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.lanmic.2026.101390","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"101390"}}