{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T23:32:14Z","timestamp":1793662334257,"version":"4.5.1"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001412","name":"Council\u00a0of\u00a0Scientific and Industrial Research, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Life Sciences"],"published-print":{"date-parts":[[2022,4]]},"DOI":"10.1016\/j.lfs.2022.120411","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T00:05:52Z","timestamp":1644969952000},"page":"120411","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":57,"special_numbering":"C","title":["Glycolytic inhibitor 2-deoxy-d-glucose attenuates SARS-CoV-2 multiplication in host cells and weakens the infective potential of progeny virions"],"prefix":"10.1016","volume":"295","author":[{"given":"Anant Narayan","family":"Bhatt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhishek","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yogesh","family":"Rai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neeraj","family":"Kumari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhiviya","family":"Vedagiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishnan H.","family":"Harshan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijayakumar","family":"Chinnadurai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudhir","family":"Chandna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"6","key":"10.1016\/j.lfs.2022.120411_bb0005","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1038\/s41577-020-0311-8","article-title":"The trinity of COVID-19: immunity, inflammation and intervention","volume":"20","author":"Tay","year":"2020","journal-title":"Nat. Rev. Immunol."},{"issue":"3","key":"10.1016\/j.lfs.2022.120411_bb0010","doi-asserted-by":"crossref","DOI":"10.29245\/2578-3009\/2020\/3.1190","article-title":"Cytokine storm and its implication in coronavirus disease 2019 (COVID-19)","volume":"4","author":"Datta","year":"2020","journal-title":"J.Immunol.Sci."},{"key":"10.1016\/j.lfs.2022.120411_bb0015","series-title":"Signal Transduction And Targeted Therapy","article-title":"COVID-19 in early 2021: current status and looking forward","volume":"Vol. 6","author":"Wang","year":"2021"},{"issue":"JULY","key":"10.1016\/j.lfs.2022.120411_bb0020","first-page":"1","article-title":"Hijacking the supplies: metabolism as a novel facet of virus-host interaction","volume":"10","author":"Mayer","year":"2019","journal-title":"Front. Immunol."},{"issue":"1","key":"10.1016\/j.lfs.2022.120411_bb0025","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s12915-019-0678-9","article-title":"Viral hijacking of cellular metabolism","volume":"17","author":"Thaker","year":"2019","journal-title":"BMC Biol."},{"issue":"3","key":"10.1016\/j.lfs.2022.120411_bb0030","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.cmet.2020.07.007","article-title":"Elevated glucose levels favor SARS-CoV-2 infection and monocyte response through a HIF-1\u03b1\/glycolysis-dependent axis","volume":"32","author":"Codo","year":"2020","journal-title":"Cell Metab."},{"issue":"7816","key":"10.1016\/j.lfs.2022.120411_bb0035","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1038\/s41586-020-2332-7","article-title":"Proteomics of SARS-CoV-2-infected host cells reveals therapy targets","volume":"583","author":"Bojkova","year":"2020","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.lfs.2022.120411_bb0040","first-page":"1","article-title":"SARS-CoV-2 hijacks folate and one-carbon metabolism for viral replication","volume":"12","author":"Zhang","year":"2021","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.lfs.2022.120411_bb0045","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12885-015-1368-9","article-title":"Transient elevation of glycolysis confers radio-resistance by facilitating DNA repair in cells","volume":"15","author":"Bhatt","year":"2015","journal-title":"BMC Cancer"},{"issue":"12","key":"10.1016\/j.lfs.2022.120411_bb0050","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1016\/j.lfs.2005.07.001","article-title":"2-Deoxyglucose: an anticancer and antiviral therapeutic, but not any more a low glucose mimetic","volume":"78","author":"Kang","year":"2006","journal-title":"Life Sci."},{"issue":"30","key":"10.1016\/j.lfs.2022.120411_bb0055","doi-asserted-by":"crossref","first-page":"E7158","DOI":"10.1073\/pnas.1800525115","article-title":"Rhinovirus induces an anabolic reprogramming in host cell metabolism essential for viral replication","volume":"115","author":"Gualdoni","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"2","key":"10.1016\/j.lfs.2022.120411_bb0060","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02175-18","article-title":"Glycolysis is an intrinsic factor for optimal replication of a norovirus","volume":"10","author":"Passalacqua","year":"2019","journal-title":"MBio"},{"issue":"4","key":"10.1016\/j.lfs.2022.120411_bb0065","doi-asserted-by":"crossref","first-page":"2358","DOI":"10.1128\/JVI.02309-14","article-title":"Dengue virus induces and requires glycolysis for optimal replication","volume":"89","author":"Fontaine","year":"2015","journal-title":"J. Virol."},{"key":"10.1016\/j.lfs.2022.120411_bb0070","series-title":"The SARS-CoV-2 transcriptional metabolic signature in lung epithelium","author":"Ehrlich","year":"2020"},{"issue":"9","key":"10.1016\/j.lfs.2022.120411_bb0075","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1099\/jgv.0.001453","article-title":"SARS-coronavirus-2 replication in vero E6 cells: replication kinetics, rapid adaptation and cytopathology","volume":"101","author":"Ogando","year":"2020","journal-title":"J.Gen.Virol."},{"issue":"13","key":"10.1016\/j.lfs.2022.120411_bb0080","doi-asserted-by":"crossref","first-page":"7001","DOI":"10.1073\/pnas.2002589117","article-title":"Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells","volume":"117","author":"Matsuyama","year":"2020","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.lfs.2022.120411_bb0085","series-title":"An inexpensive RT-PCR endpoint diagnostic assay for SARS-CoV-2 using nested PCR: direct assessment of detection efficiency of RT-qPCR tests and suitability for surveillance. bioRxiv","author":"Davda","year":"2020"},{"issue":"12","key":"10.1016\/j.lfs.2022.120411_bb0090","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1093\/glycob\/cwaa042","article-title":"Deducing the N-and O-glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2","volume":"30","author":"Shajahan","year":"2020","journal-title":"Glycobiology"},{"issue":"6501","key":"10.1016\/j.lfs.2022.120411_bb0095","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1126\/science.abb9983","article-title":"Site-specific glycan analysis of the SARS-CoV-2 spike","volume":"369","author":"Watanabe","year":"2020","journal-title":"Science"},{"key":"10.1016\/j.lfs.2022.120411_bb0100","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.61552","article-title":"Inhibition of SARS-CoV-2 viral entry upon blocking N-and O-glycan elaboration","volume":"9","author":"Yang","year":"2020","journal-title":"eLife"},{"issue":"11","key":"10.1016\/j.lfs.2022.120411_bb0105","doi-asserted-by":"crossref","first-page":"777","DOI":"10.3858\/emm.2010.42.11.079","article-title":"Endoplasmic reticulum stress (ER-stress) by 2-deoxy-D-glucose (2DG) reduces cyclooxygenase-2 (COX-2) expression and N-glycosylation and induces a loss of COX-2 activity via a Src kinase-dependent pathway in rabbit articular chondrocytes","volume":"42","author":"Yu","year":"2010","journal-title":"Exp. Mol. Med."},{"issue":"1","key":"10.1016\/j.lfs.2022.120411_bb0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2020.101992","article-title":"High-throughput rational design of the remdesivir binding site in the RdRp of SARS-CoV-2: implications for potential resistance","volume":"24","author":"Padhi","year":"2021","journal-title":"Iscience"},{"issue":"2","key":"10.1016\/j.lfs.2022.120411_bb0115","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1002\/j.2040-4603.2012.tb00424.x","article-title":"Role of monosaccharide transport proteins in carbohydrate assimilation, distribution, metabolism, and homeostasis","volume":"2","author":"Cura","year":"2011","journal-title":"Compr.Physiol."},{"issue":"5A","key":"10.1016\/j.lfs.2022.120411_bb0120","first-page":"3561","article-title":"Effect of 2-deoxy-D-glucose on various malignant cell lines in vitro","volume":"26","author":"Zhang","year":"2006","journal-title":"Anticancer Res."},{"key":"10.1016\/j.lfs.2022.120411_bb0125","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1146\/annurev.pathmechdis.3.121806.151456","article-title":"The inflammatory response to cell death","volume":"3","author":"Rock","year":"2008","journal-title":"Annu. Rev. Pathol. Mech. Dis."},{"issue":"16","key":"10.1016\/j.lfs.2022.120411_bb0130","doi-asserted-by":"crossref","first-page":"9417","DOI":"10.1074\/jbc.271.16.9417","article-title":"Mannose enters mammalian cells using a specific transporter that is insensitive to glucose","volume":"271","author":"Panneerselvam","year":"1996","journal-title":"J. Biol. Chem."},{"issue":"20","key":"10.1016\/j.lfs.2022.120411_bb0135","article-title":"Site-specific N-glycosylation characterization of recombinant SARS-CoV-2 spike proteins","volume":"19","author":"Zhang","year":"2020","journal-title":"Mol.Cell.Proteomics"},{"issue":"10","key":"10.1016\/j.lfs.2022.120411_bb0140","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1016\/j.bbagen.2019.05.012","article-title":"Exploitation of glycosylation in enveloped virus pathobiology","volume":"1863","author":"Watanabe","year":"2019","journal-title":"Biochim.Biophys.ActaGen.Subj."},{"issue":"13","key":"10.1016\/j.lfs.2022.120411_bb0145","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1002\/pros.21172","article-title":"Targeting tumor metabolism with 2-deoxyglucose in patients with castrate-resistant prostate cancer and advanced malignancies","volume":"70","author":"Stein","year":"2010","journal-title":"Prostate"},{"issue":"1","key":"10.1016\/j.lfs.2022.120411_bb0150","first-page":"1","article-title":"CellSegm-a MATLAB toolbox for high-throughput 3D cell segmentation","volume":"8","author":"Hodneland","year":"2013","journal-title":"Source CodeBiol.Med."},{"issue":"3","key":"10.1016\/j.lfs.2022.120411_bb0155","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1038\/nprot.2006.179","article-title":"Sulforhodamine B colorimetric assay for cytotoxicity screening","volume":"1","author":"Vichai","year":"2006","journal-title":"Nat. Protoc."},{"issue":"1","key":"10.1016\/j.lfs.2022.120411_bb0160","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1472-6750-5-12","article-title":"A simple technique for quantifying apoptosis in 96-well plates","volume":"5","author":"Ribble","year":"2005","journal-title":"BMC Biotechnol."},{"issue":"1","key":"10.1016\/j.lfs.2022.120411_bb0165","doi-asserted-by":"crossref","first-page":"5147","DOI":"10.1038\/s41598-021-84682-z","article-title":"Site-specific N-glycosylation analysis of animal cell culture-derived zika virus proteins","volume":"11","author":"Pralow","year":"2021","journal-title":"Sci. Rep."}],"container-title":["Life Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0024320522001114?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0024320522001114?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T20:57:42Z","timestamp":1758401862000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0024320522001114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4]]},"references-count":33,"alternative-id":["S0024320522001114"],"URL":"https:\/\/doi.org\/10.1016\/j.lfs.2022.120411","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.06.12.448175","asserted-by":"object"}]},"ISSN":["0024-3205"],"issn-type":[{"value":"0024-3205","type":"print"}],"subject":[],"published":{"date-parts":[[2022,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Glycolytic inhibitor 2-deoxy-d-glucose attenuates SARS-CoV-2 multiplication in host cells and weakens the infective potential of progeny virions","name":"articletitle","label":"Article Title"},{"value":"Life Sciences","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.lfs.2022.120411","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"120411"}}