{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:50:14Z","timestamp":1791921014097,"version":"4.3.4"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002811","name":"Cathay General Hospital","doi-asserted-by":"publisher","award":["113-CGH-FJU-07"],"award-info":[{"award-number":["113-CGH-FJU-07"]}],"id":[{"id":"10.13039\/501100002811","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006578","name":"Chi Mei Medical Center","doi-asserted-by":"publisher","award":["CLFHR11318"],"award-info":[{"award-number":["CLFHR11318"]}],"id":[{"id":"10.13039\/501100006578","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006578","name":"Chi Mei Medical Center","doi-asserted-by":"publisher","award":["113-CM-FJU-03"],"award-info":[{"award-number":["113-CM-FJU-03"]}],"id":[{"id":"10.13039\/501100006578","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006578","name":"Chi Mei Medical Center","doi-asserted-by":"publisher","award":["CMFHT 11401"],"award-info":[{"award-number":["CMFHT 11401"]}],"id":[{"id":"10.13039\/501100006578","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006578","name":"Chi Mei Medical Center","doi-asserted-by":"publisher","award":["CMFJ11206"],"award-info":[{"award-number":["CMFJ11206"]}],"id":[{"id":"10.13039\/501100006578","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013784","name":"Fu Jen Catholic University","doi-asserted-by":"publisher","award":["112-CM-FJU-03"],"award-info":[{"award-number":["112-CM-FJU-03"]}],"id":[{"id":"10.13039\/501100013784","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Life Sciences"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.lfs.2026.124606","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T15:36:11Z","timestamp":1785252971000},"page":"124606","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["TRPM8 activation by sustained-release menthol mitigates exertional heatstroke-induced testicular injury and sperm ultrastructural damage in rats"],"prefix":"10.1016","volume":"403","author":[{"given":"Ting","family":"Ruan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chien-Ming","family":"Chao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung-Min","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun-Lin","family":"Hsieh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu-Hao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling-Yu","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian-Hoe","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Jung","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching-Ping","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.lfs.2026.124606_bb0005","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1530\/rep.1.00531","article-title":"Impact of a mild scrotal heat stress on DNA integrity in murine spermatozoa","volume":"129","author":"Banks","year":"2005","journal-title":"Reproduction (Cambridge, England)."},{"key":"10.1016\/j.lfs.2026.124606_bb0010","doi-asserted-by":"crossref","first-page":"2623","DOI":"10.1007\/s00018-012-1165-4","article-title":"Heat stress response of male germ cells","volume":"70","author":"Kim","year":"2013","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.lfs.2026.124606_bb0015","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.ucl.2013.08.017","article-title":"Lifestyle, environment, and male reproductive health","volume":"41","author":"Barazani","year":"2014","journal-title":"Urol. Clin. North Am."},{"key":"10.1016\/j.lfs.2026.124606_bb0020","doi-asserted-by":"crossref","first-page":"3539","DOI":"10.1038\/s41598-021-83121-3","article-title":"Exertional heat stroke on fertility, erectile function, and testicular morphology in male rats","volume":"11","author":"Lin","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.lfs.2026.124606_bb0025","doi-asserted-by":"crossref","first-page":"2122","DOI":"10.1093\/humrep\/13.8.2122","article-title":"Occupational heat exposure and male fertility: a review","volume":"13","author":"Thonneau","year":"1998","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.lfs.2026.124606_bb0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.mce.2024.112175","article-title":"Hyperbaric oxygen preconditioning normalizes scrotal temperature, sperm quality, testicular structure, and erectile function in adult male rats subjected to exertional heat injury","volume":"584","author":"Hsieh","year":"2024","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.lfs.2026.124606_bb0035","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/S0092-8674(02)00652-9","article-title":"A TRP channel that senses cold stimuli and menthol","volume":"108","author":"Peier","year":"2002","journal-title":"Cell"},{"key":"10.1016\/j.lfs.2026.124606_bb0040","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1111\/j.1476-5381.2011.01601.x","article-title":"The thermo-TRP ion channel family: properties and therapeutic implications","volume":"165","author":"Vay","year":"2012","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.lfs.2026.124606_bb0045","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1123\/ijspp.2018-0561","article-title":"Enhancement of exercise capacity in the heat with repeated menthol-spray application","volume":"14","author":"Barwood","year":"2019","journal-title":"Int. J. Sports Physiol. Perform."},{"issue":"Suppl. 1","key":"10.1016\/j.lfs.2026.124606_bb0050","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/sms.12395","article-title":"Relieving thermal discomfort: effects of sprayed L-menthol on perception, performance, and time trial cycling in the heat","volume":"25","author":"Barwood","year":"2015","journal-title":"Scand. J. Med. Sci. Sports"},{"key":"10.1016\/j.lfs.2026.124606_bb0055","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.1096\/fj.201600257R","article-title":"Cold\/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock-induced oxidation","volume":"30","author":"Borowiec","year":"2016","journal-title":"FASEB J."},{"key":"10.1016\/j.lfs.2026.124606_bb0060","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2023.122120","article-title":"Nanocontroller-mediated dissolving hydrogel that can sustainably release cold-mimetic menthol to induce adipocyte browning for treating obesity and its related metabolic disorders","volume":"297","author":"Ruan","year":"2023","journal-title":"Biomaterials"},{"key":"10.1016\/j.lfs.2026.124606_bb0065","doi-asserted-by":"crossref","first-page":"F803","DOI":"10.1152\/ajprenal.90269.2008","article-title":"AMTB, a TRPM8 channel blocker: evidence in rats for activity in overactive bladder and painful bladder syndrome","volume":"295","author":"Lashinger","year":"2008","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"10.1016\/j.lfs.2026.124606_bb0070","doi-asserted-by":"crossref","first-page":"6304","DOI":"10.1038\/s41467-022-33835-3","article-title":"TRPM8 contributes to sex dimorphism by promoting recovery of normal sensitivity in a mouse model of chronic migraine","volume":"13","author":"Alarc\u00f3n-Alarc\u00f3n","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.lfs.2026.124606_bb0075","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.3758\/BRM.41.4.1149","article-title":"Statistical power analyses using G*Power 3.1: tests for correlation and regression analyses","volume":"41","author":"Faul","year":"2009","journal-title":"Behav. Res. Methods"},{"key":"10.1016\/j.lfs.2026.124606_bb0080","doi-asserted-by":"crossref","DOI":"10.1016\/j.lfs.2023.121640","article-title":"Myocardial structure and functional alterations in a preclinical model of exertional heat stroke","volume":"323","author":"Chao","year":"2023","journal-title":"Life Sci."},{"key":"10.1016\/j.lfs.2026.124606_bb0085","first-page":"1","article-title":"A simple and practical method for rat epididymal sperm count (Rattus norvegicus)","volume":"4","author":"Luthfi","year":"2015","journal-title":"Biol. Med. Nat. Prod. Chem."},{"key":"10.1016\/j.lfs.2026.124606_bb0090","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/j.jpurol.2014.06.005","article-title":"Evaluation of cold ischemia for preservation of testicular function during partial orchiectomy in the rat model","volume":"10","author":"McNamara","year":"2014","journal-title":"J. Pediatr. Urol."},{"key":"10.1016\/j.lfs.2026.124606_bb0095","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1186\/s13287-021-02439-x","article-title":"Combined melatonin-adipose derived mesenchymal stem cells therapy effectively protected the testis from testicular torsion-induced ischemia-reperfusion injury","volume":"12","author":"Chen","year":"2021","journal-title":"Stem Cell Res. Ther."},{"key":"10.1016\/j.lfs.2026.124606_bb0100","doi-asserted-by":"crossref","first-page":"7","DOI":"10.47162\/RJME.63.1.01","article-title":"Short histological kaleidoscope - recent findings in histology. Part I","volume":"63","author":"Constantin","year":"2022","journal-title":"Romanian J. Morphol. Embryol."},{"key":"10.1016\/j.lfs.2026.124606_bb0105","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1083\/jcb.84.2.340","article-title":"Morphometric analysis of Leydig cells in the normal rat testis","volume":"84","author":"Mori","year":"1980","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.lfs.2026.124606_bb0110","series-title":"StatPearls, StatPearls Publishing Copyright \u00a9","article-title":"Histology, Leydig cells","author":"Aladamat","year":"2026"},{"key":"10.1016\/j.lfs.2026.124606_bb0115","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-019-52192-8","article-title":"Role of indoleamine 2,3-dioxygenase in testicular immune-privilege","volume":"9","author":"Gualdoni","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.lfs.2026.124606_bb0120","series-title":"Male Reproductive Anatomy","article-title":"Testicular histopathology and spermatogenesis in mice with scrotal heat stress","author":"Dang-Cong","year":"2021"},{"key":"10.1016\/j.lfs.2026.124606_bb0125","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1098\/rstb.2010.0025","article-title":"Claudin and occludin expression and function in the seminiferous epithelium","volume":"365","author":"Morrow","year":"2010","journal-title":"Philos. Trans. R. Soc. B Biol. Sci."},{"key":"10.1016\/j.lfs.2026.124606_bb0130","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.reprotox.2004.01.002","article-title":"Sertoli cell junctional proteins as early targets for different classes of reproductive toxicants","volume":"18","author":"Fiorini","year":"2004","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.lfs.2026.124606_bb0135","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1016\/S0022-5347(01)68162-X","article-title":"Intravesical capsaicin as a treatment for refractory detrusor hyperreflexia: a dual center study with long-term followup","volume":"158","author":"De Ridder","year":"1997","journal-title":"J. Urol."},{"key":"10.1016\/j.lfs.2026.124606_bb0140","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S1474-4422(02)00072-8","article-title":"After a decade of intravesical vanilloid therapy: still more questions than answers","volume":"1","author":"Szallasi","year":"2002","journal-title":"Lancet Neurol."},{"key":"10.1016\/j.lfs.2026.124606_bb0145","first-page":"3760","article-title":"Trp-p8, a novel prostate-specific gene, is up-regulated in prostate cancer and other malignancies and shares high homology with transient receptor potential calcium channel proteins","volume":"61","author":"Tsavaler","year":"2001","journal-title":"Cancer Res."},{"key":"10.1016\/j.lfs.2026.124606_bb0150","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1097\/01.ju.0000134880.55119.cf","article-title":"Cool (TRPM8) and hot (TRPV1) receptors in the bladder and male genital tract","volume":"172","author":"Stein","year":"2004","journal-title":"J. Urol."},{"key":"10.1016\/j.lfs.2026.124606_bb0155","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbadva.2022.100069","article-title":"Temperature-dependent contractility of rat tunica dartos muscle: contribution of cold, menthol-sensitive TRPM8","volume":"3","author":"Philyppov","year":"2023","journal-title":"BBA Adv."},{"key":"10.1016\/j.lfs.2026.124606_bb0160","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1111\/j.1464-410X.1978.tb03649.x","article-title":"Absent dartos","volume":"50","author":"Shafik","year":"1978","journal-title":"Br. J. Urol."},{"key":"10.1016\/j.lfs.2026.124606_bb0165","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/978-1-4684-5913-5_5","article-title":"Epidemiologic aspects of the relationship between temperature and male reproduction","volume":"286","author":"Spira","year":"1991","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.lfs.2026.124606_bb0170","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1111\/andr.12703","article-title":"Essential roles of interstitial cells in testicular development and function","volume":"8","author":"Heinrich","year":"2020","journal-title":"Andrology"},{"key":"10.1016\/j.lfs.2026.124606_bb0175","doi-asserted-by":"crossref","first-page":"2169","DOI":"10.1016\/j.pain.2013.06.043","article-title":"TRPM8 is the principal mediator of menthol-induced analgesia of acute and inflammatory pain","volume":"154","author":"Liu","year":"2013","journal-title":"Pain"},{"key":"10.1016\/j.lfs.2026.124606_bb0180","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1186\/s12974-022-02553-4","article-title":"Activation of peripheral TRPM8 mitigates ischemic stroke by topically applied menthol","volume":"19","author":"Huang","year":"2022","journal-title":"J. Neuroinflammation"}],"updated-by":[{"DOI":"10.1016\/j.lfs.2026.124707","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:00:00Z","timestamp":1790380800000}},{"DOI":"10.1016\/j.lfs.2026.124707","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2026,11,15]],"date-time":"2026-11-15T00:00:00Z","timestamp":1794700800000}}],"container-title":["Life Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0024320526004157?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0024320526004157?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:24:52Z","timestamp":1791919492000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0024320526004157"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":36,"alternative-id":["S0024320526004157"],"URL":"https:\/\/doi.org\/10.1016\/j.lfs.2026.124606","relation":{},"ISSN":["0024-3205"],"issn-type":[{"value":"0024-3205","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"TRPM8 activation by sustained-release menthol mitigates exertional heatstroke-induced testicular injury and sperm ultrastructural damage in rats","name":"articletitle","label":"Article Title"},{"value":"Life Sciences","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.lfs.2026.124606","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"124606"}}